Original articleGeneral thoracicMethylated DLX4 Predicts Response to Pathologic Stage I Non-Small Cell Lung Cancer Resection
Section snippets
Study Population
Written informed consent was obtained from all enrolled patients, and the protocol used for this study was approved by the Institutional Review Boards. In total, 109 patients who underwent complete resection for pathologic stage I NSCLC between June 2005 and November 2011 were enrolled. The end of the follow-up period was June 2014. The mean follow-up time of all enrolled patients was 1,514 ± 660 days. Patients treated with neoadjuvant therapy were not included. Patients with large cell tumors
Association of DLX4 Methylation Status With Clinicopathologic Features
The DLX4 methylation status in cancerous specimens and adjacent normal lung tissue is shown in Figure 1A. Methylated DLX4 was also detected in LK-2 and SK-LU-1 cells (American Type Culture Collection, Rockville, MD), clearly indicating that this epigenetic modification occurs in lung cancer cell lines (Fig 1A). The overall frequency of DLX4 promoter methylation was 49.5% (54 of 109). Demographics and clinical characteristics relating to DLX4 methylation status are shown in Table 1. No
Comment
Aberrant DNA methylation of gene promoters can suppress gene expression and inactivate tumor suppressor genes, contributing to cancer initiation and progression [24]. The methylation patterns of several genes are now used as molecular markers to facilitate diagnosis, prognosis, and therapy [10]. In stage I lung cancer, putative associations between gene methylation and early recurrence have been demonstrated 7, 8, 9. Rauch and colleagues [25] demonstrated that DLX4 was methylated in more than
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Cited by (11)
Methylation status of homeobox genes in common human cancers
2016, GenomicsCitation Excerpt :The distal-less homeobox 4 (DLX4) gene is involved in cell motility and in vivo lung cancer metastasis inhibition [64]. Harada and co-workers identified DLX4 methylation as a promising biomarker for stage I NSCLC patients that is associated with poor prognosis and high risk of recurrence even after curative resection [65]. HOXA5 gene expression was identified by Zhang et al. (2015) [8] to be regulated by DNA methylation in NSCLC cells.
Epigenetic inactivation of DLX4 is associated with disease progression in chronic myeloid leukemia
2015, Biochemical and Biophysical Research CommunicationsCitation Excerpt :DNA methylation and its influence on gene expression are key factors in understanding cancer pathogenesis. Recently, hypermethylation of DLX4 has been increasingly found in several cancers including breast cancer, lung cancer, cervical cancer, and chronic lymphocytic leukemia [17–21]. However, the potential role of DLX4 methylation in regulating DLX4 expression in these cancers remains poorly studied.
Invited commentary
2015, Annals of Thoracic SurgeryDNA methylation-mediated differential expression of DLX4 isoforms has opposing roles in leukemogenesis
2022, Cellular and Molecular Biology Letters