Sato Toshitsugu 佐藤利次

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SATO Toshitsugu 佐藤利次

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Researcher Number	00390881
Affiliation (based on the past Project Information) *help	2021 – 2024: 北見工業大学, 工学部, 教授 2008 – 2019: Kitami Institute of Technology, 工学部, 准教授
Review Section/Research Field	Principal Investigator Basic Section 40020:Wood science-related / Environmental agriculture(including landscape science) / Boundary agriculture / Applied genomics Except Principal Investigator Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields
Keywords	Principal Investigator シイタケ / ラッカーゼ / 遺伝子発現抑制 / 食用担子菌 / 子実体保存性関連遺伝子 / １核菌糸 / 保存性 / lcc1 / Cas9 / ドナーベクター … More / sgRNA / 胞子 / RNP / CRISPR/Cas9 / ゲノム編集 / 遺伝子発現メカニズム / RT-PCR / ウエスタン解析 / リファレンス遺伝子 / 形質転換 / RNAi / リアルタイムPCR / デュアルプロモータ型RNAiベクター / 漆 / 遺伝子発現ベクター / 遺伝子発現系 / ウルシ / 遺伝子発現 / 発現ベクター / プロトプラスト / プロモータ / 子実体 / 発現制御 / 遺伝子 / 子実体関連遺伝子 / RNAiベクター … More Except Principal Investigator 木材腐朽菌 / キノコ / 高温耐性 / ポストハーベスト / シイタケ / ゲノム編集 / 遺伝子汚染 / 胞子 / 食用担子菌 / きのこ Less

Isolation of a safe and eco-friendly cultivar in Lentinula edodes by developing new genome editing technologies
- Principal Investigator
  
  本田与一
- Project Period (FY)
  2022 – 2025
- Research Category
  
  Grant-in-Aid for Scientific Research (A)
- Review Section
  
  Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields
- Research Institution
  Kyoto University
Establishment of Lentinula edodes genome editing technology and analysis of genes related to preservation of fruiting bodyPrincipal Investigator
- Principal Investigator
  
  Sato Toshitsugu
- Project Period (FY)
  2021 – 2023
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 40020:Wood science-related
- Research Institution
  Kitami Institute of Technology
Study on the physiological function and gene expression mechanism of Lentinula edodes laccase genes by RNAi methodPrincipal Investigator
- Principal Investigator
  
  Sato Toshitsugu
- Project Period (FY)
  2015 – 2019
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Environmental agriculture(including landscape science)
- Research Institution
  Kitami Institute of Technology
Development of an effective laccase gene expression system by the edible basidiomycete Lentinula eodesPrincipal Investigator
- Principal Investigator
  
  SATO Toshitsugu
- Project Period (FY)
  2011 – 2014
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Boundary agriculture
- Research Institution
  Kitami Institute of Technology
Construction of novel RNAi vectors for analysis of fruiting body-related genes in edible basidiomycete fungi Lentinula edodes by reverse genetic method.Principal Investigator
- Principal Investigator
  
  SATO Toshitsugu
- Project Period (FY)
  2008 – 2010
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Applied genomics
- Research Institution
  Kitami Institute of Technology

All 2024 2023 2022 2021 2019 2018 2017 2016 2014 2011 2010 2009 Other

All Journal Article Presentation Patent

[Journal Article] Development of a gene-targeting system using CRISPR/Cas9 and utilization of pyrG as a novel selectable marker in Lentinula edodes.2023
- Author(s)
  Kamiya A, Ueshima H, Nishida S, Honda Y, Kamitsuji H, Sato T, Miyamoto H, Sumita T, Izumitsu K & Irie T
- Journal Title
  
  FEMS Microbiol Lett
  
  Volume: 370 Pages: 1-6
- DOI
  10.1093/femsle/fnad042
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22K05937, KAKENHI-PROJECT-21K05700, KAKENHI-PROJECT-22H00380
[Journal Article] シイタケの遺伝子導入研究2021
- Author(s)
  佐藤利次
- Journal Title
  
  きのこ研だより
  
  Volume: 44
- Data Source
  KAKENHI-PROJECT-21K05700
[Journal Article] Overexpression and repression of the tyrosinase gene in Lentinula edodes using?the pChG vector2019
- Author(s)
  Sato Toshitsugu、Takahashi Machiko、Hasegawa Junji、Watanabe Hisayuki
- Journal Title
  
  Journal of Bioscience and Bioengineering
  
  Volume: 印刷中 Issue: 1 Pages: 1-7
- DOI
  10.1016/j.jbiosc.2018.12.013
- NAID
  40021953855
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-15K07807
[Journal Article] Overexpression of the laccase gene, lcc1, in Lentinula edodes using the pChG vector2019
- Author(s)
  Sato Toshitsugu、Suzuki Yuumi、Naito Masayoshi、Minami Aika、Suzuki Naoyuki、Yaegashi Kaori、Hirano Tatsuya
- Journal Title
  
  Mycoscience
  
  Volume: 印刷中 Issue: 4 Pages: 246-249
- DOI
  10.1016/j.myc.2019.02.004
- NAID
  40021955279
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-15K07807
[Journal Article] Heterologous expression of the Pleurotus ostreatus MnP3 gene by the laccase gene promoter in Lentinula edodes2017
- Author(s)
  Sato Toshitsugu, Irie Toshikazu, Yoshino Fumihiko
- Journal Title
  
  Bioscience, Biotechnology, and Biochemistry
  
  Volume: 81 Issue: 8 Pages: 1553-1556
- DOI
  10.1080/09168451.2017.1332977
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-15K07807, KAKENHI-PROJECT-15K07517
[Journal Article] Heterologous expression of the Pleurotus ostreatus MnP3 gene by the laccase gene promoter in Lentinula edodes2017
- Author(s)
  Toshitsugu Sato, Toshikazu Irie, Fumihiko Yoshino
- Journal Title
  
  Bioscience, Biotechnology, and Biochemistry
  
  Volume: 印刷中
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-15K07807
[Journal Article] Production of plant cell wall-degrading enzymes in Lentinula edodes and the important role of laccase in early stages of solid-state cultivation2014
- Author(s)
  Masaru NAGAI and Toshitsugu SATO
- Journal Title
  
  Mushroom Science and Biotechnology
  
  Volume: 22 Pages: 114-120
- NAID
  110009872486
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23580454
[Journal Article] Gene silencing of Lentinula edodes lcc1 gene by expression of homologous inverted repeat sequence.2011
- Author(s)
  Keiko Nakade, Yuichi Sakamoto, Hisayuki Watanabe, Toshitsugu Sato
- Journal Title
  
  Microbiol Res (in press)
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20510193
[Journal Article] Construction of novel vectors for transformation of Lentinula edodes using chitin synthase gene promoter.2011
- Author(s)
  Toshitsugu Sato, Kumiko Okawa, Tatsuya Hirano
- Journal Title
  
  J Biosci Bioeng 111
  
  Pages: 117-120
- NAID
  110008460321
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20510193
[Journal Article] Construction of novel vectors for transformation of Lentinula edodes using a chitin svnthase gene promoter.2011
- Author(s)
  Toshitsugut Sato
- Journal Title
  
  Journal Bioscience Bioengineering
  
  Volume: 111 Pages: 117-120
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20510193
[Journal Article] Cloning and characterization of Lentinula edodes class II chitin synthase gene2010
- Author(s)
  Toshitsugu Sato, Kumiko Okawa, Tatsuya Hirano
- Journal Title
  
  LeChs2. Biosci Biotechnol Biochem 74(8)
  
  Pages: 1707-1709
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20510193
[Journal Article] Cloning and characterization of Lentinula edodes class II chitin synthase gene, LeCbs2.2010
- Author(s)
  Toshitsugu Sato
- Journal Title
  
  Bioscience Biotechnology Biochemistry
  
  Volume: 74 Pages: 1707-1709
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20510193
[Journal Article] シイタケの形質転換と子実体収穫後に発現する遺伝子2009
- Author(s)
  佐藤利次
- Journal Title
  
  バイオサイエンスとインダストリー 67
  
  Pages: 316-319
- NAID
  10026194776
- Data Source
  KAKENHI-PROJECT-20510193
[Journal Article] シイタケの形質転換と子実体収穫後に発現する遺伝子2009
- Author(s)
  佐藤利次
- Journal Title
  
  バイオサイエンスとインダストリー 67巻
  
  Pages: 316-319
- NAID
  10026194776
- Data Source
  KAKENHI-PROJECT-20510193
[Journal Article] PCR subtraction, Characterization of the post-harvest changes in gene transcription in the gill of the Lentinula edodes fruiting body.2009
- Author(s)
  Yuichi Sakamoto, Keiko Nakade, Toshitsugu Sato
- Journal Title
  
  Curr Genet 55
  
  Pages: 409-423
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20510193
[Patent] プロモーター遺伝子及び発現ベクター2010
- Inventor(s)
  佐藤利次
- Industrial Property Rights Holder
  岩手県
- Acquisition Date
  2010-10-22
- Data Source
  KAKENHI-PROJECT-20510193
[Patent] プロモーター遺伝子及び発現ベクター2010
- Inventor(s)
  佐藤利次、齋藤久美子、平野達也、江井仁
- Industrial Property Rights Holder
  岩手県
- Acquisition Date
  2010-10-22
- Data Source
  KAKENHI-PROJECT-20510193
[Presentation] シイタケにおけるnon-GM型遺伝子ターゲティング系の開発2024
- Author(s)
  鳴瀧拓馬、辻本悠馬、神谷彩音、西田翔太、本田与一、佐藤利次、上辻久敏、泉津弘佑、住田卓也、入江俊一
- Organizer
  第74回日本木材学会大会
- Data Source
  KAKENHI-PROJECT-22H00380
[Presentation] シイタケにおけるダブルニッキング法を用いたゲノム編集2024
- Author(s)
  冨士元みゆ、吉澤懐、神谷彩音、稲垣翔太、本田与一、佐藤利次、上辻久敏、泉津弘佑、住田卓也、入江俊一
- Organizer
  第74回日本木材学会大会
- Data Source
  KAKENHI-PROJECT-22H00380
[Presentation] CRISPR/Cas9を用いた相同組換えによるシイタケpyrGの破壊と選択マーカー遺伝子としての利用2023
- Author(s)
  神谷彩音，上島拓輝，本田与一，佐藤利次，上辻久敏，西田翔太，稲垣翔太，泉津弘佑，住田卓也，入江俊一
- Organizer
  第73回日本木材学会大会
- Data Source
  KAKENHI-PROJECT-22H00380
[Presentation] シイタケにおけるnon-GM型遺伝子ターゲティング系の開発2023
- Author(s)
  鳴瀧拓馬、辻本悠馬、神谷彩音、西田翔太、本田与一、佐藤利次、上辻久敏、泉津弘佑、住田卓也、入江俊一
- Organizer
  第74回日本木材学会大会
- Data Source
  KAKENHI-PROJECT-21K05700
[Presentation] シイタケにおけるダブルニッキング法を用いたゲノム編集2023
- Author(s)
  冨士元みゆ、吉澤懐、神谷彩音、稲垣翔太、本田与一、佐藤利次、上辻久敏、泉津弘佑、住田卓也、入江俊一
- Organizer
  第74回日本木材学会大会
- Data Source
  KAKENHI-PROJECT-21K05700
[Presentation] CRISPR/Cas9を用いた相同組換えによるシイタケpyrGの破壊と選択マーカー遺伝子としての利用2023
- Author(s)
  神谷彩音、上島拓輝、本田与一、佐藤利次、上辻久敏、西田翔太、稲垣翔太、泉津弘佑、住田卓也、入江俊一
- Organizer
  23年度木材学会
- Data Source
  KAKENHI-PROJECT-21K05700
[Presentation] CRISPR/Cas9発現カセットの一時的導入によるシイタケPyrGの相同組換え2022
- Author(s)
  神谷彩音，上島拓輝，本田与一，佐藤利次，上辻久敏，西田翔太，稲垣翔太，泉津弘佑，住田卓也，入江俊一
- Organizer
  日本菌学会第66回大会
- Data Source
  KAKENHI-PROJECT-22H00380
[Presentation] CRISPR/Cas9発現カセットの一時的導入によるシイタケPyrGの相同組換え2022
- Author(s)
  神谷彩音、上島拓輝1、本田与一、佐藤利次、上辻久敏、西田翔太、稲垣翔太、泉津弘佑、住田卓也、入江俊一
- Organizer
  22年度日本菌学会
- Data Source
  KAKENHI-PROJECT-21K05700
[Presentation] ラッカーゼ発現に変異が生じたシイタケ組換え体のラッカーゼ遺伝子の解析2022
- Author(s)
  鈴木未由太、野地岡侑輝、佐藤利次
- Organizer
  日本農芸化学会
- Data Source
  KAKENHI-PROJECT-21K05700
[Presentation] シイタケ子実体関連遺伝子exp1の解析2022
- Author(s)
  坂本郁生、大畑早弥香、橋本侑香、吉田一生、佐藤利次
- Organizer
  日本農芸化学会
- Data Source
  KAKENHI-PROJECT-21K05700
[Presentation] シイタケ・ラッカーゼをめぐる研究 -栽培廃液から遺伝子まで－2019
- Author(s)
  佐藤　利次
- Organizer
  第69回日本木材学会大会（函館大会）・研究会（バイオマス変換研究会、きのこ研究会、及び抽出成分利用研究会の合同講演会）
- Invited
- Data Source
  KAKENHI-PROJECT-15K07807
[Presentation] 食用担子菌発酵ダイズのポリフェノール成分の解析2019
- Author(s)
  福士涼介、佐藤利次、富井さや香、高橋佑生、楊立風、山岸喬、新井博文
- Organizer
  第１２回オホーツク医学大会
- Data Source
  KAKENHI-PROJECT-15K07807
[Presentation] シイタケにおけるデュアルプロモーター型RNAiベクターの解析とexp1遺伝子発現抑制株の造成2019
- Author(s)
  大畑早弥香、佐藤利次、吉田一生、但野健太、森篤生、橋本侑香、沢目洋史
- Organizer
  第１２回オホーツク医学大会
- Data Source
  KAKENHI-PROJECT-15K07807
[Presentation] Molecular breeding of anticancer polysaccharide lentinan high content Lentinula edodes (Shiitake mushroom)2018
- Author(s)
  Toshitsugu Sato
- Organizer
  2018年韓日共同シンポジウム
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K07807
[Presentation] Bioremediation by cultivation waste water of Lentinula edodes (Shiitake mushroom) and molecular breeding of L. edodes2018
- Author(s)
  Toshitsugu Sato
- Organizer
  2018 China (Jinan) Mushroom Processing Technology International Convention
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K07807
[Presentation] pLC-hph導入シイタケ組換え株のラッカーゼ発現の解析2016
- Author(s)
  伊藤優希、山形明史、佐藤利次
- Organizer
  日本農芸化学会2016年度大会
- Place of Presentation
  札幌コンベンションセンター
- Year and Date
  2016-03-27
- Data Source
  KAKENHI-PROJECT-15K07807
[Presentation] 新規RNAiベクターを導入したシイタケ形質転換体のラッカーゼの発現2016
- Author(s)
  吉田一生、但野健太、佐藤利次
- Organizer
  日本農芸化学会2016年度大会
- Place of Presentation
  札幌コンベンションセンター
- Year and Date
  2016-03-27
- Data Source
  KAKENHI-PROJECT-15K07807
[Presentation] 食用担子菌ヒラタケのセルロース分解助長因子エクスパンシン様遺伝子Poexp5の単離2016
- Author(s)
  本間靖隆、佐々木優太、菅原大希、猪股希、佐藤利次
- Organizer
  日本農芸化学会2016年度大会
- Place of Presentation
  札幌コンベンションセンター
- Year and Date
  2016-03-27
- Data Source
  KAKENHI-PROJECT-15K07807
[Presentation] ラッカーゼ発現に変異の生じたシイタケ組換え株の解析
- Author(s)
  山形明史、伊藤優希、坂上真奈、沢目洋史、鈴木直之、佐藤利次
- Organizer
  第66回日本生物工学会大会
- Place of Presentation
  札幌コンベンションセンター
- Year and Date
  2014-09-09 – 2014-09-11
- Data Source
  KAKENHI-PROJECT-23580454
[Presentation] 2）新規RNAiベクターによるシイタケ(Lentinula edodes) lcc1遺伝子の発現抑制
- Author(s)
  但野健太、大住夏美、森篤生、沢目洋史、鈴木直之、佐藤利次
- Organizer
  第66回日本生物工学会大会
- Place of Presentation
  札幌コンベンションセンター
- Year and Date
  2014-09-09 – 2014-09-11
- Data Source
  KAKENHI-PROJECT-23580454

1. 本田与一 (70252517)

# of Collaborated Projects: 1 results

# of Collaborated Products: 4 results
2. 入江俊一 (30336721)

# of Collaborated Projects: 1 results

# of Collaborated Products: 5 results
3. 河内護之 (70771294)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
4. 中沢威人 (80608141)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
5. 上辻久敏 (90455527)

# of Collaborated Projects: 1 results

# of Collaborated Products: 4 results

Sato Toshitsugu 佐藤 利次

SATO Toshitsugu 佐藤 利次

Research Projects

Research Products

Co-Researchers

Isolation of a safe and eco-friendly cultivar in Lentinula edodes by developing new genome editing technologies

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Establishment of Lentinula edodes genome editing technology and analysis of genes related to preservation of fruiting bodyPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Study on the physiological function and gene expression mechanism of Lentinula edodes laccase genes by RNAi methodPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Development of an effective laccase gene expression system by the edible basidiomycete Lentinula eodesPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Construction of novel RNAi vectors for analysis of fruiting body-related genes in edible basidiomycete fungi Lentinula edodes by reverse genetic method.Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

[Journal Article] Development of a gene-targeting system using CRISPR/Cas9 and utilization of pyrG as a novel selectable marker in Lentinula edodes.2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] シイタケの遺伝子導入研究2021

Author(s)

Journal Title

Data Source

[Journal Article] Overexpression and repression of the tyrosinase gene in Lentinula edodes using?the pChG vector2019

Author(s)

Journal Title

DOI

NAID

Data Source

[Journal Article] Overexpression of the laccase gene, lcc1, in Lentinula edodes using the pChG vector2019

Author(s)

Journal Title

DOI

NAID

Data Source

[Journal Article] Heterologous expression of the Pleurotus ostreatus MnP3 gene by the laccase gene promoter in Lentinula edodes2017

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Heterologous expression of the Pleurotus ostreatus MnP3 gene by the laccase gene promoter in Lentinula edodes2017

Author(s)

Journal Title

Data Source

[Journal Article] Production of plant cell wall-degrading enzymes in Lentinula edodes and the important role of laccase in early stages of solid-state cultivation2014

Author(s)

Journal Title

NAID

Data Source

[Journal Article] Gene silencing of Lentinula edodes lcc1 gene by expression of homologous inverted repeat sequence.2011

Author(s)

Journal Title

Data Source

[Journal Article] Construction of novel vectors for transformation of Lentinula edodes using chitin synthase gene promoter.2011

Author(s)

Journal Title

NAID

Data Source

[Journal Article] Construction of novel vectors for transformation of Lentinula edodes using a chitin svnthase gene promoter.2011

Sato Toshitsugu 佐藤利次

SATO Toshitsugu 佐藤利次