KUROSAWA Hiroshi 黒澤尋

… Alternative Names

黒澤尋クロサワヒロシ

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Researcher Number	10225295
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Affiliation (Current)	2025: 山梨大学, その他部局等, 理事
Affiliation (based on the past Project Information) *help	2019 – 2022: 山梨大学, 大学院総合研究部, 教授 2014 – 2016: 山梨大学, 総合研究部, 教授 2011 – 2013: 山梨大学, 医学工学総合研究部, 教授 2011: 山梨大学, 大学院・医学工総合研究部, 教授 2009 – 2011: University of Yamanashi, 大学院・医学工学総合研究部, 教授 … More 2007: University of Yamanashi, Department of Research Interdisciplinary Graduate School of Medicine and Engineering, Associate Professor 2006: 山梨大学, 大学院医学工学総合研究部, 助教授 2003 – 2004: 山梨大学, 大学院・医学工学総合研究部, 助教授 1995 – 2003: 山梨大学, 工学部, 助教授 Less
Review Section/Research Field	Principal Investigator Biofunction/Bioprocess / 生物・生体工学 / Basic Section 27040:Biofunction and bioprocess engineering-related / Biofunction/Bioprocess Except Principal Investigator 生物・生体工学 / Basic Section 27040:Biofunction and bioprocess engineering-related / Pediatrics / Biomedical engineering/Biological material science
Keywords	Principal Investigator 胚様体 / 未分化維持 / ES細胞 / ES cell / embryoid body / 低酸素 / 未分化性維持 / ヒトiPS細胞 / アゴニスト活性 / bFGF受容体 … More / 二量体化 / 環状ペプチド / 熱安定性 / bFGF代替物 / standardization / gene expression / deifferentiation / EB formation method / quantitative PCR / 発現遺伝子 / RT-PCR / 規格化 / 遺伝子発現 / 細胞分化 / 胚様体形成法 / 定量PCR / cardiomyocyte / differentiation / high-oxygen / low-oxygen / alkaline phosphatase / undifferentiated / 拍動 / 培養法 / ハンギングドロップ / 細胞外マトリックス / MPC / アルカリフォスターゼ / 心筋 / 分化誘導 / 高酸素 / アルカリフォスファターゼ / 同調培養 / 細胞周期 / 細胞均一化 / 多能性関連遺伝子 / 心筋分化 / ヒトｉＰＳ細胞 / 細胞周期同調 / ノコダゾール / リン脂質ポリマー / 表面・界面物性 / 生体材料 / 発生・分化 / 細胞・組織 / 再生医学 / 再生医療 / バイオ生産プロセス / 高密度培養 / 細胞支持体 / 肝細胞 / 人工肝臓 / 乳酸菌 / Thiobacillus / ATPase / 耐酸性 … More Except Principal Investigator 組織工学 / 細胞培養工学 / アメリカ合衆国 / 再生医療 / Thiobacillus thiooxidans / 硫黄酸化細菌 / 日米比較 / 産業化 / 非凍結保管 / ヒトiPS細胞 / 細胞分化 / シグナル伝達 / 分子ネットワーク / 分子経路 / Membrane-bound cytochrome c / Cytochrome c oxidase / Axid Tolerance / シトクロム c オキシダーゼ / シトクロムcオキシダーゼ / シトクロムC / シトクロムCオキシダーゼ / 耐酸性 / Sulfate / Acid phosphatase / 硫酸 / 酸性ホスファターゼ / トランスレーショナルリサーチ / 発現制御 / 脳神経疾患 / ゲノム / 治療 / 遺伝子 / iPS / 神経疾患 / 自閉症 / バルプロ酸ナトリウム / 化合物 / 修復治療 / 遺伝子発現 / MeCP2 / レット症候群 / エピゲノム / エピジェネティクス / 足場 / マテリアル / デバイス / バイオミメティックス / 生物工学 / リアクター / ベンチャービジネス / アンケート調査 Less

ヒトiPS細胞に有効で熱安定性の高い環状ペプチド型bFGF代替物の創製Principal Investigator
- Principal Investigator
  
  黒澤尋
- Project Period (FY)
  2021 – 2022
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 27040:Biofunction and bioprocess engineering-related
- Research Institution
  University of Yamanashi
ヒトiPS細胞の革新的な非凍結密閉系保存技術の開発
- Principal Investigator
  
  大貫喜嗣
- Project Period (FY)
  2021 – 2023
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 27040:Biofunction and bioprocess engineering-related
- Research Institution
  University of Yamanashi
細胞非自律的に作用する遺伝子の網羅的同定法開発
- Principal Investigator
  
  升井伸治
- Project Period (FY)
  2019 – 2021
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 27040:Biofunction and bioprocess engineering-related
- Research Institution
  University of Yamanashi
Development of a novel culture method for obtaining human iPS cells with high pluripotencyPrincipal Investigator
- Principal Investigator
  
  KUROSAWA Hiroshi
- Project Period (FY)
  2014 – 2016
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Biofunction/Bioprocess
- Research Institution
  University of Yamanashi
Development epigenomic restoration therapy for autistic disorders
- Principal Investigator
  
  KUBOTA Takeo
- Project Period (FY)
  2011 – 2013
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  Pediatrics
- Research Institution
  University of Yamanashi
Control of the differentiation pluripotent stem cells(ES・iPS cells) by culture surface coated with functional compounds.Principal Investigator
- Principal Investigator
  
  KUROSAWA Hiroshi
- Project Period (FY)
  2009 – 2011
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Biofunction/Bioprocess
- Research Institution
  University of Yamanashi
Standardization of embryoid body formation from embryonic stem cellsand quality estimation of embryoid bodiesPrincipal Investigator
- Principal Investigator
  
  KUROSAWA Hiroshi
- Project Period (FY)
  2006 – 2007
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Biofunction/Bioprocess
- Research Institution
  University of Yamanashi
組織工学的アプローチによる新規バイオミメティック組織デバイス開発の現状と課題
- Principal Investigator
  
  高木睦
- Project Period (FY)
  2003
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Biomedical engineering/Biological material science
- Research Institution
  Osaka University
The elucidation of the physiochemical factor of the mouse ES cell on the ambryoid body formation.Principal Investigator
- Principal Investigator
  
  KUROSAWA Hiroshi
- Project Period (FY)
  2003 – 2004
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Biofunction/Bioprocess
- Research Institution
  University of Yamanashi
ティッシュエンジニアリングによる組織再生の医療応用における工学的課題調査
- Principal Investigator
  
  高木睦
- Project Period (FY)
  2002
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  生物・生体工学
- Research Institution
  Osaka University
化学・生物工学的観点からの組織工学のポテンシャル評価-再生医療技術に関する分野横断的研究動向の調査-
- Principal Investigator
  
  高木睦
- Project Period (FY)
  2001
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  生物・生体工学
- Research Institution
  Osaka University
肝細胞の簡便迅速な高密度充填が可能な新規細胞支持基材を用いた人工肝臓の開発Principal Investigator
- Principal Investigator
  
  黒澤尋
- Project Period (FY)
  1999 – 2000
- Research Category
  
  Grant-in-Aid for Encouragement of Young Scientists (A)
- Research Field
  
  生物・生体工学
- Research Institution
  University of Yamanashi
Elucidation of acid tolerant function of Thiobacillus thiooxidans grown under scid and extreme environment
- Principal Investigator
  
  AMANO Yoshifumi
- Project Period (FY)
  1997 – 1998
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  生物・生体工学
- Research Institution
  Yamanashi University
細菌由来の耐酸性因子を再構成して得られる酸防御機構を備えた生体模擬システムPrincipal Investigator
- Principal Investigator
  
  黒澤尋
- Project Period (FY)
  1996
- Research Category
  
  Grant-in-Aid for Encouragement of Young Scientists (A)
- Research Field
  
  生物・生体工学
- Research Institution
  University of Yamanashi
Elucidation of sulfate-dependent acid phosphatase from Thiobacillus thiooxidans and determination of sulfate in waters using whole cells
- Principal Investigator
  
  AMANO Yoshifumi
- Project Period (FY)
  1995 – 1996
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  生物・生体工学
- Research Institution
  Yamanashi University

All 2022 2021 2020 2019 2016 2014 2013 2011 2010 2009 2007 2005 2004

All Journal Article Presentation

[Journal Article] Optimized conditions for the supplementation of human-induced pluripotent stem cell cultures with a GSK-3 inhibitor during embryoid body formation with the aim of inducing differentiation into mesodermal and cardiac lineage2020
- Author(s)
  Yoda Kiyomi、Ohnuki Yoshitsugu、Masui Shinji、Kurosawa Hiroshi
- Journal Title
  
  Journal of Bioscience and Bioengineering
  
  Volume: 129 Issue: 3 Pages: 371-378
- DOI
  10.1016/j.jbiosc.2019.09.015
- NAID
  40022194562
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19K05169
[Journal Article] Comparison of genomic and epigenomic expression in monozygotic twins discordant for Rett syndrome2013
- Author(s)
  Miyake K, Yang C, Minakuchi Y, Ohori K, Soutome M, Hirasawa T, Kazuki Y, Adachi N, Suzuki S, Itoh M, Goto Y, Andoh T, Kurosawa H, Akamatsu W, Oyama M, Okano H, Oshimura M, Sasaki M, Toyoda A, Kubota T
- Journal Title
  
  PLoS ONE
  
  Volume: 8 Issue: 6 Pages: e66729-e66729
- DOI
  10.1371/journal.pone.0066729
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23240062, KAKENHI-PROJECT-23390272, KAKENHI-PROJECT-23591491, KAKENHI-PROJECT-25670473, KAKENHI-PROJECT-25860852
[Journal Article] Comparison of genomic and epigenomic expression in monozygotic twins discordant for Rett syndrome2013
- Author(s)
  Miyake K, Yang C, Minakuchi Y, OhoriK, Soutome M, HirasawaT, KazukiY, Adachi N, SuzukiS, ItohM, GotoY, AndohT, KurosawaH, Akamatsu W, Oyama M, Okano H, Oshimura M, Sasaki M, Toyoda A, Kubota T
- Journal Title
  
  PLoS ONE
  
  Volume: 8
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23390272
[Journal Article] Effect of glucose concentration during embryoid body(EB) formation from mouse embryonic stem cells on EB growth and cell differentiation2011
- Author(s)
  Mochizuki, H., Ohnuki, Y., Kurosawa, H.
- Journal Title
  
  J. Biosc. Bioeng.
  
  Volume: 111 Pages: 92-97
- NAID
  110008440356
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21560809
[Journal Article] Characterization of embryoid bodies of mouse embryonic stem cells formed under various culture conditions and estimation of differentiation status of such bodies2007
- Author(s)
  Mikiko, Koike, Shujiro, Sakaki, Yoshifumi, Amano, Hiroshi Kurosawa
- Journal Title
  
  Journal of Bioscience and Bioengineering Vol.104, No.4
  
  Pages: 294-299
- NAID
  110006437010
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-18560751
[Journal Article] Methods for inducing embryoid body formation: In vitro differentiation system of embryonic stem cells.2007
- Author(s)
  Hiroshi Kurosawa
- Journal Title
  
  Journal of Bioscience and Bioengineering 103
  
  Pages: 389-398
- NAID
  110006278720
- Description
  「研究成果報告書概要(和文)」より
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-18560751
[Journal Article] Methods for inducing embryoid body formation : In vitro differentiation system of embryonic stem cells2007
- Author(s)
  Hiroshi, Kurosawa
- Journal Title
  
  Journal of Bioscience and Bioengineering Vol.103, No.5
  
  Pages: 389-398
- NAID
  110006278720
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-18560751
[Journal Article] A round-bottom 96-well polystyrene plate coated with 2-methacryloyloxyethyl phosphoryicholine as an effective tool for embryoid body formation.2005
- Author(s)
  Mikiko Koike, Hiroshi Kurosawa, Yoshifumi Amano
- Journal Title
  
  Cytotechnology (in press)
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-15560675
[Journal Article] A round-bottom 96-well polystyrene plate coated with 2-methacryloyloxyethyl phosphorylcholine as an effective tool for embryoid body formation2005
- Author(s)
  Mikiko Koike, Hiroshi Kurosawa, Yoshifumi Amano
- Journal Title
  
  Cytotechnology (印刷中)
- Description
  「研究成果報告書概要(和文)」より
- Data Source
  KAKENHI-PROJECT-15560675
[Journal Article] A novel culture method for the formation of embryoid body of mouse embryonic stem cell.2004
- Author(s)
  Hiroshi Kurosawa, Mikiko Koike, Yoshifumi Amano
- Journal Title
  
  Reports of the Faculty of Engineering (University of Yamanashi) No.52
  
  Pages: 23-29
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-15560675
[Journal Article] マウスES細胞の胚様体形成のための新規培養技術2004
- Author(s)
  黒澤尋, 小池美紀子, 天野義文
- Journal Title
  
  山梨大学工学部研究報告 52
  
  Pages: 23-29
- NAID
  110000952233
- Description
  「研究成果報告書概要(和文)」より
- Data Source
  KAKENHI-PROJECT-15560675
[Presentation] 保存培地のpH がヒトiPS 細胞の新規非凍結保存安定性に及ぼす影響2022
- Author(s)
  佐藤佑菜, 徐卓然, 大貫喜嗣, 田中佑治, 黒澤尋
- Organizer
  日本生物工学会
- Data Source
  KAKENHI-PROJECT-21K04786
[Presentation] Novel cyclic N-alkyl peptides as bFGF Mimetics to Maintain the Pluripotency of Human Induced Pluripotent Stem Cells2021
- Author(s)
  Masashi Sato, Hiromi Tanaka, Yoshitsugu Ohnuki, Shinji Masui, Takashi Kawakami and Hiroshi Kurosawa
- Organizer
  The 93rd Annual Meeting, Japanese Tissue Culture Association
- Data Source
  KAKENHI-PROJECT-21K04785
[Presentation] Screening of molecules that facilitate transition from mouse embryonic stem cells to early-embryonic-like cells2020
- Author(s)
  Aoi Murakami, Yoshitsugu Ohnuki, Shinji Masui, Hiroshi Kurosawa
- Organizer
  JAACT2020 Fuchu
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K05169
[Presentation] Primitive endoderm cells promote proliferation and differentiation of pluripotent stem cells2020
- Author(s)
  Takumi Iwama, Yoshitsugu Ohnuki, Shinji Masui, Hiroshi Kurosawa
- Organizer
  JAACT2020 Fuchu
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K05169
[Presentation] Novel Cyclic N-alkyl Peptides as BFGF Mimetics to Maintain the Pluripotency of Human Induced Pluripotent Stem Cells2020
- Author(s)
  Masashi Sato, Hiromi Tanaka, Yoshitsugu Ohnuki, Shinji Masui, Takashi Kawakami, Hiroshi Kurosawa
- Organizer
  JAACT2020 Fuchu
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K05169
[Presentation] ヒトiPS細胞の未分化増殖を可能にする新規ペプチド化合物の分子進化工学的スクリーニング法による探索及び人工 FGFRアゴニストとしての機能解析2019
- Author(s)
  佐藤将、大貫喜嗣、升井伸治、川上隆史、黒澤尋
- Organizer
  第71回日本生物工学会大会
- Data Source
  KAKENHI-PROJECT-19K05169
[Presentation] ヒトiPS細胞において中胚葉分化が促進された胚様体を形成するためのGSK-3β阻害剤処理条件の検討2019
- Author(s)
  依田聖未、大貫喜嗣、升井伸治、黒澤尋
- Organizer
  日本組織培養学会第92回大会
- Data Source
  KAKENHI-PROJECT-19K05169
[Presentation] ノコダゾール処理によるヒトｉＰＳ細胞の心筋分化誘導2016
- Author(s)
  山口千尋、大貫喜嗣、黒澤尋
- Organizer
  第68回日本生物工学会大会
- Place of Presentation
  富山国際会議場（富山県富山市）
- Data Source
  KAKENHI-PROJECT-26420794
[Presentation] Nocodazole処理によるヒトiPS細胞の細胞周期同調2014
- Author(s)
  山口千尋、大貫喜嗣、黒澤　尋
- Organizer
  第66回日本生物工学会大会
- Place of Presentation
  札幌コンベンションセンター（札幌市）
- Year and Date
  2014-09-10
- Data Source
  KAKENHI-PROJECT-26420794
[Presentation] Astrocytes are increased in neural cells derived from Rett syndrome iPS cells2013
- Author(s)
  Ando T, Akamatsu W, Matusmoto, Mikake K, Yamaguchi R, Okada Y, Imaizumi Y, Ohyama M, Kurosawa H, Amagai M, Kubota T, Okano H
- Organizer
  第35回日本神経科学大会
- Place of Presentation
  名古屋
- Year and Date
  2013-07-21
- Data Source
  KAKENHI-PROJECT-23390272
[Presentation] 小児自閉症疾患患者に由来するiPS細胞の樹立とその遺伝学的・神経学的解析2011
- Author(s)
  久保田健夫, 安藤友子, 黒澤尋, 岡野栄之
- Organizer
  平成23年度やまなし産学官連携研究交流事業
- Place of Presentation
  甲府
- Year and Date
  2011-09-09
- Data Source
  KAKENHI-PROJECT-23390272
[Presentation] 培養器材がマウスES細胞の胚様体分化に及ぼす影響2010
- Author(s)
  大貫喜嗣、黒澤尋
- Organizer
  第9回日本再生医療学会総会
- Place of Presentation
  広島国際会議場
- Year and Date
  2010-03-18
- Data Source
  KAKENHI-PROJECT-21560809
[Presentation] ハンギングドロップ法による胚様体形成における初期細胞数がマウスES細胞分化に及ぼす影響2010
- Author(s)
  大貫喜嗣、黒澤尋
- Organizer
  第62回日本生物工学会大会
- Place of Presentation
  宮崎シーガイア
- Year and Date
  2010-10-28
- Data Source
  KAKENHI-PROJECT-21560809
[Presentation] マウスES細胞の胚様体分化に及ぼす培養面コート剤の影響2009
- Author(s)
  大貫喜嗣、黒澤尋
- Organizer
  第61回日本生物工学会大会
- Place of Presentation
  名古屋大学
- Year and Date
  2009-09-24
- Data Source
  KAKENHI-PROJECT-21560809
[Presentation] Graphical presentation of gene expression pattern in various types of embryoid bodies from mouse ES cells2007
- Author(s)
  Mikiko, Koike, Shujiro, Sakaki, Yoshifumi, Amano, Hiroshi, Kurosawa
- Organizer
  Annual Meeting 2007 The Society for Biotechnology (Japan)
- Place of Presentation
  Hiroshima University, Hiroshima
- Year and Date
  2007-09-25
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-18560751
[Presentation] Optimization of cystic embryoid body (EB) formation from mouse ES cells and expression of marker genes in differentiating EBs2007
- Author(s)
  Emiko, Yasuda, Yuji, Seki, Shuiiro, Sakaki. Hiroshi, Kurosawa
- Organizer
  Annual Meeting 2007 The Society for Biotechnology(Japan)
- Place of Presentation
  Hiroshima University. Hiroshima
- Year and Date
  2007-09-25
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-18560751
[Presentation] Graphical presentation of gene expression pattern in various types of embryoid bodies from mouse ES cells2007
- Author(s)
  Mikiko, Koike, Shujiro, Sakaki, Yoshifumi, Amano, Hiroshi, Kurosawa
- Organizer
  TERMIS AP 2007
- Place of Presentation
  Tokyo
- Year and Date
  2007-12-04
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-18560751

1. 高木睦 (20263212)

# of Collaborated Projects: 3 results

# of Collaborated Products: 0 results
2. 寺田聡 (60311685)

# of Collaborated Projects: 3 results

# of Collaborated Products: 0 results
3. 上平正道 (40202022)

# of Collaborated Projects: 3 results

# of Collaborated Products: 0 results
4. 酒井康行 (00235128)

# of Collaborated Projects: 3 results

# of Collaborated Products: 0 results
5. 山地秀樹 (40283874)

# of Collaborated Projects: 3 results

# of Collaborated Products: 0 results
6. AMANO Yoshifumi (70020401)

# of Collaborated Projects: 2 results

# of Collaborated Products: 0 results
7. KUBOTA Takeo (70293511)

# of Collaborated Projects: 1 results

# of Collaborated Products: 4 results
8. OKANO Hideyuki (60160694)

# of Collaborated Projects: 1 results

# of Collaborated Products: 3 results
9. NAGASE Hiroki (90322073)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
10. NAKAMURA Kazuo (80111780)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
11. 望月和樹 (80423838)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
12. 升井伸治 (20342850)

# of Collaborated Projects: 1 results

# of Collaborated Products: 8 results
13. 斉藤美佳子 (20291346)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
14. 若山照彦 (40360672)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
15. 川上隆史 (60638881)

# of Collaborated Projects: 1 results

# of Collaborated Products: 1 results
16. 大貫喜嗣 (70759315)

# of Collaborated Projects: 1 results

# of Collaborated Products: 1 results

KUROSAWA Hiroshi 黒澤 尋

黒澤 尋 クロサワ ヒロシ

Research Projects

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ヒトiPS細胞に有効で熱安定性の高い環状ペプチド型bFGF代替物の創製Principal Investigator

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Development of a novel culture method for obtaining human iPS cells with high pluripotencyPrincipal Investigator

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Control of the differentiation pluripotent stem cells(ES・iPS cells) by culture surface coated with functional compounds.Principal Investigator

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Standardization of embryoid body formation from embryonic stem cellsand quality estimation of embryoid bodiesPrincipal Investigator

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The elucidation of the physiochemical factor of the mouse ES cell on the ambryoid body formation.Principal Investigator

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肝細胞の簡便迅速な高密度充填が可能な新規細胞支持基材を用いた人工肝臓の開発Principal Investigator

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細菌由来の耐酸性因子を再構成して得られる酸防御機構を備えた生体模擬システムPrincipal Investigator

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KUROSAWA Hiroshi 黒澤尋

黒澤尋クロサワヒロシ