Miyamoto Kazuhide 宮本和英

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MIYAMOTO Kazuhide 宮本和英

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Researcher Number	10415317
Affiliation (Current)	2025: 山陽小野田市立山口東京理科大学, 薬学部, 教授
Affiliation (based on the past Project Information) *help	2020 – 2023: 山陽小野田市立山口東京理科大学, 薬学部, 教授 2011 – 2019: 姫路獨協大学, 薬学部, 准教授
Review Section/Research Field	Principal Investigator Basic Section 50020:Tumor diagnostics and therapeutics-related / Bio-related chemistry / Tumor diagnostics / Medical systems Except Principal Investigator Laboratory medicine
Keywords	Principal Investigator ユビキチン化 / ユビキチン / バイオマーカ / がん診断 / 癌診断 / バイオマーカー / 診断 / がん / ARF / E2活性 / ユビキチンリガーゼ / 診断システムユビキチン化 / 検査 … More Except Principal Investigator … More ユビキチンープロテアソーム系 / プロテアソーム阻害剤 / ユビキチン-プロテアソーム系 / ユビキチン化酵素 / M蛋白 / 多発性骨髄腫 / ユビキチン-プロテアソーム / ERストレス / PML/RARA Less

Development of a detecting system of ubiquitination and verification in mouse tumor-bearing modelsPrincipal Investigator
- Principal Investigator
  
  Miyamoto Kazuhide
- Project Period (FY)
  2021 – 2023
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 50020:Tumor diagnostics and therapeutics-related
- Research Institution
  Tokyo University of Science, Yamaguchi
Rapid and accurate detection of ubiquitination using artificial ligasePrincipal Investigator
- Principal Investigator
  
  Miyamoto Kazuhide
- Project Period (FY)
  2017 – 2020
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Bio-related chemistry
- Research Institution
  Tokyo University of Science, Yamaguchi
  Himeji Dokkyo University
Evaluation of M-protein related ubiquitin enzyme as a new biomarker for Multiple Myeloma
- Principal Investigator
  
  Mariko Takenokuchi
- Project Period (FY)
  2016 – 2018
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Laboratory medicine
- Research Institution
  Himeji Dokkyo University
Highly sensitive detection of ubiquitination using tumor-bearing mouse modelPrincipal Investigator
- Principal Investigator
  
  Miyamoto Kazuhide
- Project Period (FY)
  2014 – 2016
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Tumor diagnostics
- Research Institution
  Himeji Dokkyo University
Evaluation of serum ubiquitin enzyme as a new biomarker for PML-RARA leukemia
- Principal Investigator
  
  Takenokuchi Mariko
- Project Period (FY)
  2013 – 2015
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Laboratory medicine
- Research Institution
  Himeji Dokkyo University
High sensitive detection of ubiquitination reaction in urinePrincipal Investigator
- Principal Investigator
  
  MIYAMOTO Kazuhide
- Project Period (FY)
  2011 – 2012
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Medical systems
- Research Institution
  Himeji Dokkyo University

[Book] Zinc Finger Proteins (Methods and Protocols)2018
- Author(s)
  Kazuhide Miyamoto* et al. (Editor Jia Liu)
- Total Pages
  276
- Publisher
  Springer Nature
- Data Source
  KAKENHI-PROJECT-17K05942
[Journal Article] Detection of E2 Activity on Lateral Flow Immunochromatography with Artificial E3s2024
- Author(s)
  Ayumi Fukuda, Takashi Tadokoro, and Kazuhide Miyamoto*
- Journal Title
  
  Peptide Science
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21K07197
[Journal Article] Unique E2-binding specificity of artificial RING fingers in cancer cells2024
- Author(s)
  Miyamoto Kazuhide*、Tadokoro Takashi、Matsumoto Atsushi
- Journal Title
  
  Scientific Reports
  
  Volume: 14 Issue: 1 Pages: 2545-2545
- DOI
  10.1038/s41598-024-52793-y
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-21K07197
[Journal Article] Artificial <scp>RING</scp> finger reveals unique auto‐ubiquitination with <scp>E2</scp> specificity2023
- Author(s)
  Miyamoto Kazuhide*、Matsumoto Atsushi
- Journal Title
  
  Protein Science
  
  Volume: 32 Issue: 10
- DOI
  10.1002/pro.4766
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21K07197
[Journal Article] Analysis of E2-Binding Capabilities of Artificial E3s by Fluorescence Spectroscopies2022
- Author(s)
  Kazuhide Miyamoto*, Hinako Ishii, and Takashi Tadokoro
- Journal Title
  
  Peptide Science
  
  Pages: 61-62
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21K07197
[Journal Article] Creation of Artificial E3s with Specific E2-binding Capabilities by Amino Acid Replacements2021
- Author(s)
  Ayumi Fukuda, Kazuhide Miyamoto
- Journal Title
  
  Peptide Science
  
  Pages: 85-86
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21K07197
[Journal Article] Solution structure of the zinc finger domain of human RNF144A ubiquitin ligase2020
- Author(s)
  Kazuhide Miyamoto*, Kaori Migita, and Kazuki Saito
- Journal Title
  
  Protein Science
  
  Volume: 29 Issue: 8 Pages: 1836-1842
- DOI
  10.1002/pro.3903
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K05942
[Journal Article] Detection of Ubiquitination Activities using ARF on Micro Bioactive Analyzer2020
- Author(s)
  Kazuhide Miyamoto
- Journal Title
  
  Peptide Science
  
  Volume: -
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K05942
[Journal Article] Preparation of the silkmoth prothoracicotropic hormone receptor, Torso, a receptor tyrosine kinase with a novel dimer structure.2019
- Author(s)
  Airi Uechi, Shoko Nakamichi, Yuma Yamabana, Mayumi Sunagawa, Yuri Ishigaki, Kazuhide Miyamoto, Kazuki Saito
- Journal Title
  
  Pept. Sci.
  
  Pages: 32-32
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K05942
[Journal Article] Exploring amino acid residues for regulating E2 specificities of artificial RING fingers.2019
- Author(s)
  Ayumi Yamashita, Kazuki Saito, Kazuhide Miyamoto*
- Journal Title
  
  Pept. Sci.
  
  Pages: 63-63
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K05942
[Journal Article] Unique RING finger structure from the human HRD1 protein2019
- Author(s)
  Miyamoto Kazuhide、Taguchi Yukari、Saito Kazuki
- Journal Title
  
  Protein Science
  
  Volume: 28 Issue: 2 Pages: 448-453
- DOI
  10.1002/pro.3532
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K05942
[Journal Article] Zinc finger domain of the human DTX protein adopts a unique RING fold2019
- Author(s)
  Miyamoto Kazuhide、Fujiwara Yuma、Saito Kazuki
- Journal Title
  
  Protein Science
  
  Volume: 28 Issue: 6 Pages: 1151-1156
- DOI
  10.1002/pro.3610
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K05942
[Journal Article] Detection of ubiquitination activities of artificial RING fingers in human breast cancer cells.2019
- Author(s)
  Kazuhide Miyamoto*, Arisa Nakatani, Mayumi Sunagawa, Kazuki Saito
- Journal Title
  
  Pept. Sci.
  
  Pages: 62-62
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K05942
[Journal Article] Preparation of the silkmoth prothoracicotropic hormone receptor, Torso, which is a receptor tyrosine kinase with novel dimer structure.2018
- Author(s)
  Airi Uechi, Shoko Nakamichi, Yuma Yamabana, Mayumi Sunagawa, Yuri Ishigaki, Kazuhide Miyamoto, Kazuki Saito*
- Journal Title
  
  Peptide Science
  
  Pages: 32-32
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K05942
[Journal Article] Detection of E2 activities in cancer cells using an artificial E3 ligase2018
- Author(s)
  Ayumi Yamashita, Kazuki Saito, Kazuhide Miyamoto
- Journal Title
  
  Pept. Sci.
  
  Pages: 152-153
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K05942
[Journal Article] Design of a system for monitoring ubiquitination activities of E2 enzymes using engineered RING finger proteins2018
- Author(s)
  Kazuhide Miyamoto*, Kazuki Saito
- Journal Title
  
  Methods in Molecular Biology
  
  Volume: 1867 Pages: 75-87
- DOI
  10.1007/978-1-4939-8799-3_6
- ISBN
  9781493987986, 9781493987993
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K05942
[Journal Article] Solution structure of the PHD finger from the human KIAA1045 protein2018
- Author(s)
  Kazuhide Miyamoto, Ayumi Yamashita, Kazuki Saito
- Journal Title
  
  Protein Science
  
  Volume: 27 Issue: 5 Pages: 903-1012
- DOI
  10.1002/pro.3389
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K05942
[Journal Article] Cellular effects of artificial ubiquitin ligases on cancer cells.2018
- Author(s)
  Mayumi Sunagawa, Ayumi Yamashita, Kazuki Saito, Kazuhide Miyamoto
- Journal Title
  
  Mayumi Sunagawa, Ayumi Yamashita, Kazuki Saito, Kazuhide Miyamoto*:
  
  Pages: 124-125
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K05942
[Journal Article] Unique auto-ubiquitination activities of artificial RING fingers in cancer cells2018
- Author(s)
  4.Kazuhide Miyamoto*, Arisa Nakatani, Mayumi Sunagawa, and Kazuki Saito
- Journal Title
  
  Protein Science
  
  Volume: 27(9) Issue: 9 Pages: 1704-1709
- DOI
  10.1002/pro.3452
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K05942
[Journal Article] Concise machinery for monitoring ubiquitination activities using novel artificial RING fingers2018
- Author(s)
  Miyamoto Kazuhide、Saito Kazuki
- Journal Title
  
  Protein Science (Invited Review)
  
  Volume: 27 Issue: 8 Pages: 1354-1363
- DOI
  10.1002/pro.3427
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K05942
[Journal Article] Exploring amino acid residues for regulating E2 specificity of artificial RING fingers2018
- Author(s)
  Ayumi Yamashita, Kazuki Saito, and Kazuhide Miyamoto*
- Journal Title
  
  Peptide Science
  
  Pages: 63-63
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K05942
[Journal Article] Detection of ubiquitination activities of artificial RING fingers in human breast cancer cells.2018
- Author(s)
  Kazuhide Miyamoto*, Arisa Nakatani, Mayumi Sunagawa, Kazuki Saito
- Journal Title
  
  Peptide Science
  
  Pages: 62-62
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K05942
[Journal Article] The zinc finger domain of RING finger protein 141 reveals a unique RING fold2017
- Author(s)
  Kazuhide Miyamoto, Airi Uechi, Kazuki Saito
- Journal Title
  
  Protein Science
  
  Volume: 26 Issue: 8 Pages: 1681-1686
- DOI
  10.1002/pro.3201
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K05942
[Journal Article] Development of Molecular Design Strategy of Artificial Ubiquitin-ligases — Toward Cancer Diagnosis Based on Ubiquitination Activities —2017
- Author(s)
  宮本和英, 砂川真弓, 齋藤一樹
- Journal Title
  
  BUNSEKI KAGAKU
  
  Volume: 66 Issue: 6 Pages: 393-402
- DOI
  10.2116/bunsekikagaku.66.393
- NAID
  130005746056
- ISSN
  0525-1931
- Language
  Japanese
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-17K05942
[Journal Article] 人工ユビキチンリガーゼの分子設計法の開発とその応用2017
- Author(s)
  宮本和英，砂川真弓,　齋藤一樹
- Journal Title
  
  分析化学（総合論文）
  
  Volume: -
- Peer Reviewed / Acknowledgement Compliant / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-26430147
[Journal Article] The unique N‐terminal zinc finger of synaptotagmin‐like protein 4 reveals FYVE structure2017
- Author(s)
  Kazuhide Miyamoto, Arisa Nakatani, Kazuki Saito
- Journal Title
  
  Protein Science
  
  Volume: 26 Issue: 12 Pages: 2451-2457
- DOI
  10.1002/pro.3301
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K05942
[Journal Article] Highly sensitive detection of E2 activity in ubiquitination using an artificial RING finger2017
- Author(s)
  Miyamoto K, Sumida M, Yuasa-Sunagawa M, Saito K
- Journal Title
  
  J Pept Sci.
  
  Volume: 23 Issue: 3 Pages: 222-227
- DOI
  10.1002/psc.2972
- Peer Reviewed / Acknowledgement Compliant / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-26430147
[Journal Article] Improved isolation procedure for shikonin from the root of the Chinese medicinal plant Lithospermum erythrorhizon and its solubilization with cyclodextrins2016
- Author(s)
  Hideki Azuma, Jiawei Li, Ryota Youda, Toshio Suzuki, Kazuhide Miyamoto, Taizo Taniguchi, Takeshi Nagasaki
- Journal Title
  
  J. Appl. Res. Med. Arom Plants
  
  Volume: 3 Issue: 2 Pages: 58-63
- DOI
  10.1016/j.jarmap.2016.01.002
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-26350528, KAKENHI-PROJECT-26430147
[Journal Article] Rapid and accurate monitoring of E2 activities using an artificial E3 ligase on a signal accumulation ISFET biosensor2016
- Author(s)
  Miyamoto K, Sumida M, Sunagawa M
- Journal Title
  
  Peptide Science
  
  Volume: - Pages: 171-172
- Peer Reviewed / Acknowledgement Compliant / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-26430147
[Journal Article] The Artifical Ubiquitin Ligase E3 Inhibits Cellular Proliferation in Cancer Cells2016
- Author(s)
  Sunagawa M, Nakatani A, Miyamoto K
- Journal Title
  
  Peptide Science
  
  Volume: - Pages: 125-126
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-26430147
[Journal Article] Bortezomib Causes ER Stress-related Death of Acute Promyelocytic Leukemia Cells Through Excessive Accumulation of PML-RARA2015
- Author(s)
  Mariko Takenokuchi, Kazuhide Miyamoto, Katsuyasu Saigo, Taizo Taniguchi
- Journal Title
  
  ANTICANCER RESEARCH
  
  Volume: 35(6) Pages: 3307-3316
- Peer Reviewed / Acknowledgement Compliant
- Data Source
  KAKENHI-PROJECT-25460711
[Journal Article] Structural model of ubiquitin transfer onto an artificial RING finger as an E3 ligase2014
- Author(s)
  Kazuhide Miyamoto
- Journal Title
  
  Scientific Reports
  
  Volume: 4 Issue: 1 Pages: 6574-6574
- DOI
  10.1038/srep06574
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-26430147
[Journal Article] アレルギー性皮膚炎の治療薬－シコニンの効果―2013
- Author(s)
  Watanabe A, Hichiri H, Takenokuchi M, Miyamoto K, Kadoyama K, Taniguchi T
- Journal Title
  
  Small Animal Dermatology
  
  Volume: 9 Pages: 60-65
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23700588
[Journal Article] T アレルギー性皮膚炎の治療薬-シコニンの効果2013
- Author(s)
  Watanabe A, Hichiri H, Takenokuchi M, Miyamoto K, Kadoyama K, Taniguchi
- Journal Title
  
  Small Animal Dermatology
  
  Volume: 9 Pages: 60-65
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23700588
[Journal Article] Oligopeptides derived from autophosphorylation sites of EGF receptor suppress EGF-stimulated responses in human lung carcinoma A549 cells.2013
- Author(s)
  Kuroda Y, Kato-Kogoe N, Tasaki E, Murata M, Ueda K, Abe M, Miyamoto K, Nakase I, Futaki S, Tohyama Y, Hirose M
- Journal Title
  
  Eur J Pharmacol
  
  Volume: 698 Issue: 1-3 Pages: 87-94
- DOI
  10.1016/j.ejphar.2012.10.007
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22390297, KAKENHI-PROJECT-22590076, KAKENHI-PROJECT-23590374, KAKENHI-PROJECT-23593077, KAKENHI-PROJECT-23700588, KAKENHI-PROJECT-23790129
[Journal Article] Ubiquitination of an artificial RING finger without a substrate and a tag2012
- Author(s)
  K. Miyamoto
- Journal Title
  
  Journal of Peptide Science
  
  Volume: 18 Issue: 2 Pages: 135-139
- DOI
  10.1002/psc.1426
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23700588
[Journal Article] Esophageal cancer exhibits resistance to a novel IGF-1R inhibitor NVP-AEW541 with maintained RAS-MAPK activity.2012
- Author(s)
  Bao, X.H
- Journal Title
  
  Anticancer Res
  
  Volume: 32 Issue: 3 Pages: 2827-2834
- DOI
  10.1159/000218708
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23390323, KAKENHI-PROJECT-25460711
[Journal Article] TogiyaSolution structure of LC4 transmembrane segment of CCR52011
- Author(s)
  K. Miyamoto and K.
- Journal Title
  
  PLoS One
  
  Volume: 6 Issue: 5 Pages: e20452-e20452
- DOI
  10.1371/journal.pone.0020452
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23700588
[Patent] 人工合成疑似Ｅ３を有効成分とする、癌診断薬、抗癌剤選択支援剤、および天然Ｅ３に拮抗する阻害剤2015
- Inventor(s)
  宮本和英，(株)行医研
- Industrial Property Rights Holder
  宮本和英，(株)行医研
- Industrial Property Rights Type
  特許
- Filing Date
  2015-08-05
- Acquisition Date
  2016-08-19
- Data Source
  KAKENHI-PROJECT-26430147
[Patent] キメラタンパク質（E2との選択的結合活性を有し標的タンパク質の必要なしにタンパク質のユビキチン化活性を示すタンパク質の提供）2015
- Inventor(s)
  宮本和英，(株)行医研
- Industrial Property Rights Holder
  宮本和英，(株)行医研
- Industrial Property Rights Type
  特許
- Filing Date
  2015-03-02
- Acquisition Date
  2016-07-22
- Data Source
  KAKENHI-PROJECT-26430147
[Presentation] ラテラルフローイムノクロマトグラフィーを用いたユビキチン結合酵素の活性検出2023
- Author(s)
  福田歩未，田所高志、○宮本和英
- Organizer
  第35回バイオメディカル分析科学シンポジウム
- Data Source
  KAKENHI-PROJECT-21K07197
[Presentation] DETECTION OF E2 ACTIVITY ON LATERAL FLOW IMMNOCHROMATOGRAPHY WITH ARTIFICIAL E3S2023
- Author(s)
  福田歩未，田所高志、○宮本和英
- Organizer
  第60回ペプチド討論会
- Data Source
  KAKENHI-PROJECT-21K07197
[Presentation] イムノクロマトグラフィーを用いたユビキチン結合酵素の活性の検出2023
- Author(s)
  ○福田歩未，村上侑美、田所高志、宮本和英
- Organizer
  第29回中国四国支部分析化学若手セミナー
- Data Source
  KAKENHI-PROJECT-21K07197
[Presentation] 蛍光標識した人工的なRING Finger のE2結合能の解析2022
- Author(s)
  石井陽菜子、田所高志、宮本和英
- Organizer
  34回バイオメディカル分析科学シンポジウム
- Data Source
  KAKENHI-PROJECT-21K07197
[Presentation] ANALYSIS OF E2-BINDING CAPABILITIES OF ARTIFICIAL E3S BY FLUORESCENCE SPECTROSCOPIES2022
- Author(s)
  宮本和英、石井陽菜子、田所高志
- Organizer
  第59回ペプチド討論会
- Data Source
  KAKENHI-PROJECT-21K07197
[Presentation] CREATION OF ARTIFICIAL E3S WITH SPECIFIC E2-BINDING CAPABILITIES BY AMINO ACID REPLACEMENTS2021
- Author(s)
  福田歩未、宮本和英
- Organizer
  第58回ペプチド討論会
- Data Source
  KAKENHI-PROJECT-21K07197
[Presentation] Detection of ubiquitination activities in cancer cells using artificial RING fingers2021
- Author(s)
  Kazuhide Miyamoto
- Organizer
  65TH Annual Meeting of Biophysical Society
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] Detection of ubiquitination activities using ARF on micro Bioactive analyzer2020
- Author(s)
  宮本和英
- Organizer
  第57回ペプチド討論会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 人工分子ARFを活用するユビキチン化活性の検出法2020
- Author(s)
  宮本和英, 中谷有沙, 黒涼菜, 齋藤一樹
- Organizer
  分析イノベーション交流会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 乳がん細胞膜中のユビキチン結合酵素活性の検出2019
- Author(s)
  宮本和英
- Organizer
  第78回日本癌学会学術総会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 複雑なジスルフィド構造を有するカイコガ前胸腺刺激ホルモンの大量調製法の確立　～分泌型発現系を備えたブレビバチルス菌の利用～2019
- Author(s)
  下田佳苗, 石垣悠里, 此上祥史, 片岡宏誌, 宮本和英, 齋藤一樹
- Organizer
  第66回日本生化学会近畿支部例会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 人工分子ARFを用いるユビキチン結合酵素活性の検出2019
- Author(s)
  宮本和英，中谷有沙、山下歩美、齋藤一樹
- Organizer
  日本薬学会139年会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 人工分子ARFを活用したユビキチン結合酵素活性の特異的検出2019
- Author(s)
  黒涼菜, 田口由加里, 齋藤一樹, 宮本和英
- Organizer
  第32回バイオメディカル分析シンポジウム
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 人工分子ARFを活用した乳がん細胞のユビキチン化活性検出2019
- Author(s)
  宮本和英, 黒涼菜, 田口由加里, 中谷有沙, 齋藤一樹
- Organizer
  第32回バイオメディカル分析シンポジウム
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] Auto-ubiquitination of artificial ring fingers in human breast cancer cells.2019
- Author(s)
  宮本和英, 中谷有沙, 田口由加里, 齋藤一樹
- Organizer
  第56回ペプチド討論会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 人工ユビキチンリガーゼを活用した乳がん細胞のE2活性検出2019
- Author(s)
  宮本和英, 中谷有沙, 齋藤一樹
- Organizer
  第79回分析化学討論会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 人工分子ARFが有するユビキチン結合酵素の特異的認識能の改変2019
- Author(s)
  田口由加里、齋藤一樹、宮本和英
- Organizer
  日本薬学会139年会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] ブレビバチルス菌を用いたカイコガ前胸腺刺激ホルモンの分泌発現とその構造確認2019
- Author(s)
  下田佳苗, 石垣悠里, 此上祥史, 宮本和英, 片岡宏誌, 齋藤一樹
- Organizer
  第92回日本生化学会大会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] あらかじめジスルフィド架橋で二量化したチロシンキナーゼ型レセプター・Torsoの大量調製2019
- Author(s)
  中道祥子，上地愛理，山端裕真，石垣悠里，宮本和英，齋藤一樹
- Organizer
  日本薬学会139年会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 自己ユビキチン化能を有する人工RING fingerの作製法2019
- Author(s)
  宮本和英, 中谷有沙, 齋藤一樹
- Organizer
  第71回日本生物工学会大会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] これまでにはなかった二量化様式のチロシンキナーゼ型レセプター・Torsoの大量調製法の確立2018
- Author(s)
  上地愛理, 中道祥子, 砂川真弓, 宮本和英, 齋藤一樹
- Organizer
  第68回日本薬学会近畿支部総会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] Molecular design of artificial RING fingers for detecting ubiquitination activities.2018
- Author(s)
  Kazuhide Miyamoto, Ayumi Yamashita, Kazuki Saito
- Organizer
  62nd Annual Meeting of the Biophysical Society
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 膜貫通領域のジスルフィド架橋で二量化しているチロシンキナーゼ型レセプターTorso の大量調製2018
- Author(s)
  上地愛理，砂川真弓，宮本和英，齋藤一樹
- Organizer
  日本生化学会大会近畿支部
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] Unique ubiquitination reaction of artificial E3 ligases in cancer cells2018
- Author(s)
  宮本和英、山下美歩、齋藤一樹
- Organizer
  第77回日本癌学会学術総会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 人工ユビキチンリガーゼのE2特異性を担うアミノ酸残基の探索2018
- Author(s)
  山下歩美，齋藤一樹，宮本和英
- Organizer
  第31回バイオメディカル分析科学シンポジウム
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] Preparation of the silkmoth prothoracicotropic hormone receptor, Torso, which is a receptor tyrosine kinase with novel dimer structure.2018
- Author(s)
  Airi Uechi, Shoko Nakamichi, Yuma Yamabana, Mayumi Sunagawa, Yuri Ishigaki, Kazuhide Miyamoto, Kazuki Saito
- Organizer
  10th International Peptide Symposium
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] Detection of ubiquitination activities of artificial RING fingers in human breast cancer cells.2018
- Author(s)
  Kazuhide Miyamoto, Arisa Nakatani, Mayumi Sunagawa, Kazuki Saito
- Organizer
  10th International Peptide Symposium
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] ブレビバチルス菌を用いたカイコガ前胸腺刺激ホルモンの大量調製法の確立～複雑なジスルフィド構造を有するリコンビナントタンパク質の発現・調製～2018
- Author(s)
  石垣悠里, 下田佳苗, 此上祥史, 片岡宏誌, 宮本和英, 齋藤一樹
- Organizer
  第68回日本薬学会近畿支部総会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 人工ユビキチンリガーゼを用いる特異的なE2活性検出の検討2018
- Author(s)
  宮本和英、山下歩美、齋藤一樹
- Organizer
  第78回分析化学討論会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] Exploring amino acid residues for regulating E2 specificity of artificial RING fingers2018
- Author(s)
  Ayumi Yamashita, Kazuki Saito, and Kazuhide Miyamoto
- Organizer
  10th International Peptide Symposium
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 細胞膜貫通領域のジスルフィド架橋を介して二量化しているチロシンキナーゼ型レセプター・Torsoの大量調製2018
- Author(s)
  上地愛理，中道祥子，山端裕真，砂川真弓，石垣悠里，宮本和英，齋藤一樹
- Organizer
  第35回関西ペプチドセミナー
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 乳がん細胞のユビキチン化に関与するE2活性の検出2018
- Author(s)
  宮本和英，中谷有沙、齋藤一樹
- Organizer
  第8回新アミノ酸分析研究会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 人工RINGフィンガーが有するE2特異的認識能の検討2018
- Author(s)
  山下歩美, 田口由加里, 齋藤一樹, 宮本和英
- Organizer
  第68回日本薬学会近畿支部総会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 人工ユビキチンリガーゼを活用するがん診断への挑戦2018
- Author(s)
  宮本和英
- Organizer
  日本薬学会第138年会一般シンポジウム「がん診断・治療に貢献する次世代分析法開発への挑戦」【シンポジウム主催】
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 複雑なジスルフィド構造を有するカイコガ前胸腺刺激ホルモンPTTHの、ブレビバチルス菌を用いた大量調製2018
- Author(s)
  石垣悠里、下田佳苗、此上祥史、片岡宏誌、宮本和英、齋藤一樹
- Organizer
  第91回日本生化学会大会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 人工ユビキチンリガーゼを用いたがん細胞のユビキチン化活性の測定2017
- Author(s)
  砂川真弓, 中谷有沙, 齋藤一樹, 宮本和英
- Organizer
  第67回日本薬学会近畿支部総会・大会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 人工ユビキチンリガーゼを活用したユビキチン化活性の検出2017
- Author(s)
  山下歩美, 齋藤一樹, 宮本和英
- Organizer
  新アミノ酸分析研究会第7回学術講演会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 人工ユビキチンリガーゼのがん細胞における効果2017
- Author(s)
  砂川真弓, 山下歩美, 齋藤一樹, 宮本和英
- Organizer
  第54回ペプチド討論会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 人工RINGフィンガーを用いた簡便なE2活性の検出法2017
- Author(s)
  山下歩美, 齋藤一樹, 宮本和英
- Organizer
  第34回関西地区ペプチドセミナー
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 人工E3リガーゼのユビキチン化反応に基づくがんのスクリーニング2017
- Author(s)
  宮本和英
- Organizer
  第76回日本癌学会学術総会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] Measuring E2 activities by artificial RING fingers in cancer cells.2017
- Author(s)
  Mayumi Sunagawa, Arisa Nakatani, Kazuki Saito, Kazuhide Miyamoto
- Organizer
  Consortium of Biological Sciences 2017 (ConBio2017)
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] 人工E3リガーゼを用いるがん細胞のE2活性の検出2017
- Author(s)
  山下歩美, 齋藤一樹, 宮本和英
- Organizer
  第54回ペプチド討論会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] ユビキチン化反応におけるE2活性のリアルタイム検出法2017
- Author(s)
  宮本
- Organizer
  超異分野学会大阪フォーラム2017
- Place of Presentation
  立命館大学
- Year and Date
  2017-03-11
- Invited
- Data Source
  KAKENHI-PROJECT-26430147
[Presentation] ユビキチン化活性の検出に用いる人工ユビキチンリガーゼの設計法2017
- Author(s)
  宮本和英, 砂川真弓, 齋藤一樹
- Organizer
  第77回分析化学討論会
- Data Source
  KAKENHI-PROJECT-17K05942
[Presentation] Creation of an artificial E3 ligase and its anticancer effects2016
- Author(s)
  宮本、砂川、中谷
- Organizer
  第39回日本分子生物学会
- Place of Presentation
  パシフィコ横浜
- Year and Date
  2016-11-30
- Data Source
  KAKENHI-PROJECT-26430147
[Presentation] 人工ユビキチンリガーゼの分子設計法の構築2016
- Author(s)
  宮本和英
- Organizer
  第33回関西ペプチドセミナー
- Place of Presentation
  近畿大学
- Year and Date
  2016-11-26
- Invited
- Data Source
  KAKENHI-PROJECT-26430147
[Presentation] ユビキチン結合酵素（E2）活性の検出に基づく腫瘍サイズの決定2016
- Author(s)
  宮本、砂川、齋藤
- Organizer
  第75回日本癌学会学術総会
- Place of Presentation
  パシフィコ横浜
- Year and Date
  2016-10-06
- Data Source
  KAKENHI-PROJECT-26430147
[Presentation] 人工ユビキチンリガーゼを用いた血清中のユビキチン化反応の検出法2016
- Author(s)
  宮本、砂川、齋藤
- Organizer
  第29回バイオメディカル分析科学シンポジウム
- Place of Presentation
  京都大学
- Year and Date
  2016-09-02
- Data Source
  KAKENHI-PROJECT-26430147
[Presentation] 人工ユビキチンリガーゼによる急性前骨髄性白血病の抑制効果2016
- Author(s)
  宮本、中谷、砂川、齋藤、
- Organizer
  第33回関西ペプチドセミナー
- Place of Presentation
  近畿大学
- Year and Date
  2016-11-26
- Data Source
  KAKENHI-PROJECT-26430147
[Presentation] Real-time detection of E2 activities in the ubiquitination reaction using an artificial E3 ligase2016
- Author(s)
  宮本、澄田、砂川
- Organizer
  第39回日本分子生物学会
- Place of Presentation
  パシフィコ横浜
- Year and Date
  2016-11-30
- Data Source
  KAKENHI-PROJECT-26430147
[Presentation] 癌などの重篤疾患の治療前診断法2016
- Author(s)
  宮本
- Organizer
  企業・大学・学生マッチング
- Place of Presentation
  兵庫県立大
- Year and Date
  2016-05-28
- Data Source
  KAKENHI-PROJECT-26430147
[Presentation] The artificial ubiquitin ligase E3 inhibits cellular proliferation in cancer cells2016
- Author(s)
  宮本、砂川、中谷
- Organizer
  第53回ペプチド討論会
- Place of Presentation
  京都テルサ
- Year and Date
  2016-10-26
- Data Source
  KAKENHI-PROJECT-26430147
[Presentation] 新規抗がん剤としての人工ユビキチンリガーゼの設計とその作用機序2016
- Author(s)
  宮本、砂川、齋藤
- Organizer
  第66回日本薬学会近畿支部
- Place of Presentation
  大阪薬科大学
- Year and Date
  2016-10-15
- Data Source
  KAKENHI-PROJECT-26430147
[Presentation] Rapid and accurate monitoring of E2 activities using an artificial E3 ligase on a signal accumulation ISFET biosensor2016
- Author(s)
  宮本、澄田、砂川
- Organizer
  第53回ペプチド討論会
- Place of Presentation
  京都テルサ
- Year and Date
  2016-10-26
- Data Source
  KAKENHI-PROJECT-26430147
[Presentation] 人工ユビキチンリガーゼのがん細胞増殖抑制効果2016
- Author(s)
  宮本、中谷、砂川、齋藤
- Organizer
  第29回バイオメディカル分析科学シンポジウム
- Place of Presentation
  京都大学
- Year and Date
  2016-09-02
- Data Source
  KAKENHI-PROJECT-26430147
[Presentation] 人工ユビキチンリガーゼを用いるユビキチン結合酵素（E2）活性の検出法2016
- Author(s)
  宮本、砂川、齋藤
- Organizer
  第65回日本分析化学会
- Place of Presentation
  北海道大学
- Year and Date
  2016-09-14
- Data Source
  KAKENHI-PROJECT-26430147
[Presentation] Structural analysis of ubiquitin transfer onto an artificial E3 ligase2015
- Author(s)
  宮本和英
- Organizer
  第38回日本分子生物学会
- Place of Presentation
  神戸国際会議場（神戸市）
- Year and Date
  2015-12-02
- Data Source
  KAKENHI-PROJECT-26430147
[Presentation] 癌などの重篤疾患の治療前診断法2015
- Author(s)
  宮本和英
- Organizer
  国際フロンティア産業メッセ
- Place of Presentation
  神戸国際会議場（神戸市）
- Year and Date
  2015-09-04
- Data Source
  KAKENHI-PROJECT-26430147
[Presentation] 癌などの重篤疾患の治療前診断法2015
- Author(s)
  宮本和英
- Organizer
  企業・大学・学生マッチング
- Place of Presentation
  兵庫県立大（姫路市）
- Year and Date
  2015-05-28
- Data Source
  KAKENHI-PROJECT-26430147
[Presentation] ユビキチンプロテアソームと疾患診断法2015
- Author(s)
  宮本和英
- Organizer
  メディカルジャパン2015
- Place of Presentation
  インテックス大阪（大阪）
- Year and Date
  2015-02-06
- Invited
- Data Source
  KAKENHI-PROJECT-26430147
[Presentation] ユビキチン化を用いた新しい診断法2014
- Author(s)
  宮本和英
- Organizer
  国際フロンティア産業メッセ
- Place of Presentation
  神戸国際展示場（神戸）
- Year and Date
  2014-09-14
- Data Source
  KAKENHI-PROJECT-26430147
[Presentation] ユビキチン化を用いた新しい診断法2014
- Author(s)
  宮本和英
- Organizer
  企業・大学・学生マッチング
- Place of Presentation
  兵庫県立大（姫路）
- Year and Date
  2014-05-29
- Data Source
  KAKENHI-PROJECT-26430147
[Presentation] Ubiquitination on an artificial RING finger as an E3 ligase2014
- Author(s)
  宮本和英
- Organizer
  第51回ペプチド討論会
- Place of Presentation
  徳島大（徳島）
- Year and Date
  2014-10-22
- Data Source
  KAKENHI-PROJECT-26430147
[Presentation] An artificial ubiquitin-ligating (E3) enzyme by the method of alpha helical region substitution2012
- Author(s)
  Miyamoto K
- Organizer
  第35回日本分子生物学会
- Place of Presentation
  福岡国際会議場(九州）
- Data Source
  KAKENHI-PROJECT-23700588
[Presentation] An artificial ubiquitin-ligating (E3) enzyme by the method of alpha helical region substitution2012
- Author(s)
  Miyamoto K
- Organizer
  第35回日本分子生物学会
- Place of Presentation
  福岡国際会議場(九州)
- Year and Date
  2012-12-11
- Data Source
  KAKENHI-PROJECT-23700588
[Presentation] Creation of an artificial RING finger without a substrate and a tag2011
- Author(s)
  宮本和英
- Organizer
  第48回ペプチド討論会
- Place of Presentation
  札幌コンベンションセンター（北海道）
- Data Source
  KAKENHI-PROJECT-23700588
[Presentation] Creation of an artificial RING finger without a substrate and a tag2011
- Author(s)
  Miyamoto K
- Organizer
  第48回ペプチド討論会
- Place of Presentation
  札幌コンベンションセンター(北海道)
- Year and Date
  2011-09-28
- Data Source
  KAKENHI-PROJECT-23700588

1. Takenokuchi Mariko (10379430)

# of Collaborated Projects: 3 results

# of Collaborated Products: 1 results
2. TANIGUCHI Taizo (70346253)

# of Collaborated Projects: 3 results

# of Collaborated Products: 1 results
3. SAIGO Katsuyasu (20304107)

# of Collaborated Projects: 2 results

# of Collaborated Products: 1 results
4. 砂川真弓 (40444509)

# of Collaborated Projects: 1 results

# of Collaborated Products: 6 results
5. AZUMA Hideki

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
6. 長崎健

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
7. 廣瀬宗孝

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results

Miyamoto Kazuhide 宮本 和英

MIYAMOTO Kazuhide 宮本 和英

Research Projects

Research Products

Co-Researchers

Development of a detecting system of ubiquitination and verification in mouse tumor-bearing modelsPrincipal Investigator

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Rapid and accurate detection of ubiquitination using artificial ligasePrincipal Investigator

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Evaluation of M-protein related ubiquitin enzyme as a new biomarker for Multiple Myeloma

Principal Investigator

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Highly sensitive detection of ubiquitination using tumor-bearing mouse modelPrincipal Investigator

Principal Investigator

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Evaluation of serum ubiquitin enzyme as a new biomarker for PML-RARA leukemia

Principal Investigator

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High sensitive detection of ubiquitination reaction in urinePrincipal Investigator

Principal Investigator

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[Book] Zinc Finger Proteins (Methods and Protocols)2018

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Total Pages

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[Journal Article] Detection of E2 Activity on Lateral Flow Immunochromatography with Artificial E3s2024

Author(s)

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[Journal Article] Unique E2-binding specificity of artificial RING fingers in cancer cells2024

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Artificial <scp>RING</scp> finger reveals unique auto‐ubiquitination with <scp>E2</scp> specificity2023

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Journal Title

DOI

Data Source

[Journal Article] Analysis of E2-Binding Capabilities of Artificial E3s by Fluorescence Spectroscopies2022

Author(s)

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Data Source

[Journal Article] Creation of Artificial E3s with Specific E2-binding Capabilities by Amino Acid Replacements2021

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[Journal Article] Solution structure of the zinc finger domain of human RNF144A ubiquitin ligase2020

Author(s)

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Data Source

[Journal Article] Detection of Ubiquitination Activities using ARF on Micro Bioactive Analyzer2020

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[Journal Article] Preparation of the silkmoth prothoracicotropic hormone receptor, Torso, a receptor tyrosine kinase with a novel dimer structure.2019

Author(s)

Journal Title

Miyamoto Kazuhide 宮本和英

MIYAMOTO Kazuhide 宮本和英

[Journal Article] Development of Molecular Design Strategy of Artificial Ubiquitin-ligases — Toward Cancer Diagnosis Based on Ubiquitination Activities —2017