UENO HIROSHI 上野博史

… Alternative Names

Ueno Hiroshi 上野博史

UENO Hiroshi 上野博史

上野博史ウエノヒロシ

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Researcher Number	10546592
Other IDs
Affiliation (Current)	2025: 東京大学, 大学院工学系研究科(工学部), 講師
Affiliation (based on the past Project Information) *help	2021 – 2024: 東京大学, 大学院工学系研究科(工学部), 講師 2016 – 2020: 東京大学, 大学院工学系研究科(工学部), 助教 2013 – 2014: 中央大学, 理工学部, 助教 2011: 中央大学, 理工学部, 助教
Review Section/Research Field	Principal Investigator Complex systems / Biophysics / Medium-sized Section 43:Biology at molecular to cellular levels, and related fields / Basic Section 43040:Biophysics-related Except Principal Investigator Medium-sized Section 43:Biology at molecular to cellular levels, and related fields / Mathematical physics/Fundamental condensed matter physics
Keywords	Principal Investigator 1分子計測 / 進化分子工学 / ATPase / マイクロデバイス / 分子機械 / 進化 / 分子モーター / 構造解析 / 祖先配列推定 / F1-ATPase … More / H+/ATP比 / 構造 / エネルギー変換 / ATP合成酵素 / 回転分子モーター / ゆらぎ / 酵素 / 揺らぎ / １分子計測 / イオン輸送 / 1分子計測・操作 … More Except Principal Investigator エネルギー変換 / 分子モーター / イオン駆動力 / イオンチャネル / F1-ATPase / 統計力学 Less

Creation of ATP synthase with multiple Fo torque generating unitsPrincipal Investigator
- Principal Investigator
  
  上野博史
- Project Period (FY)
  2024 – 2026
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 43040:Biophysics-related
- Research Institution
  The University of Tokyo
Elucidation of the evolution of the rotary catalysis mechanism of F1-ATPase by reconstruction of the ancestral enzymePrincipal Investigator
- Principal Investigator
  
  上野博史
- Project Period (FY)
  2023 – 2024
- Research Category
  
  Grant-in-Aid for Challenging Research (Exploratory)
- Review Section
  
  Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
- Research Institution
  The University of Tokyo
固定子イオンチャネル型モーターの作動機構の解明
- Principal Investigator
  
  今田勝巳
- Project Period (FY)
  2023 – 2024
- Research Category
  
  Grant-in-Aid for Challenging Research (Exploratory)
- Review Section
  
  Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
- Research Institution
  Osaka University
新規on-chipスクリーニング技術による分子機械の機能改変Principal Investigator
- Principal Investigator
  
  上野博史
- Project Period (FY)
  2021 – 2022
- Research Category
  
  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
- Review Section
  
  Complex systems
- Research Institution
  The University of Tokyo
生体分子の改造・創成を実現する新規進化分子工学的スクリーニング技術の開発Principal Investigator
- Principal Investigator
  
  上野博史
- Project Period (FY)
  2019 – 2020
- Research Category
  
  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
- Review Section
  
  Complex systems
- Research Institution
  The University of Tokyo
酵素１分子の活性揺らぎと進化能の関係Principal Investigator
- Principal Investigator
  
  上野博史
- Project Period (FY)
  2018 – 2019
- Research Category
  
  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
- Research Institution
  The University of Tokyo
Elucidation of rotary ion transport mechanism of V-ATPase by visualization and control of substratePrincipal Investigator
- Principal Investigator
  
  Ueno Hiroshi
- Project Period (FY)
  2016 – 2018
- Research Category
  
  Grant-in-Aid for Challenging Exploratory Research
- Research Field
  
  Biophysics
- Research Institution
  The University of Tokyo
Elucidation of operation mechanism of rotary molecular motors by comparison with the mechanisms of F1 and V1-ATPases.Principal Investigator
- Principal Investigator
  
  UENO Hiroshi
- Project Period (FY)
  2013 – 2014
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Biophysics
- Research Institution
  Chuo University
分子モーターの1分子トラジェクトリからポテンシャルを描く
- Principal Investigator
  
  鳥谷部祥一
- Project Period (FY)
  2011
- Research Category
  
  Grant-in-Aid for Challenging Exploratory Research
- Research Field
  
  Mathematical physics/Fundamental condensed matter physics
- Research Institution
  Chuo University

[Book] FoF1モーター　「DOJIN BIOSCIENCE SERIES 17　1分子生物学」2014
- Author(s)
  上野博史, 鳥谷部祥一, 宗行英朗
- Total Pages
  13
- Publisher
  化学同人
- Data Source
  KAKENHI-PROJECT-25840053
[Journal Article] The series of conformational states adopted by rotorless F1-ATPase during its hydrolysis cycle2024
- Author(s)
  Sobti Meghna、Ueno Hiroshi、Brown Simon H.J.、Noji Hiroyuki、Stewart Alastair G.
- Journal Title
  
  Structure
  
  Volume: 32 Issue: 4 Pages: 393-399.e3
- DOI
  10.1016/j.str.2023.12.014
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K18092
[Journal Article] Engineering of IF<sub>1</sub>‐susceptive bacterial F<sub>1</sub>‐ATPase2024
- Author(s)
  Hatasaki Yuichiro C.、Kobayashi Ryohei、Watanabe Ryo R.、Hara Mayu、Ueno Hiroshi、Noji Hiroyuki
- Journal Title
  
  Protein Science
  
  Volume: 33 Issue: 4 Pages: 1-12
- DOI
  10.1002/pro.4942
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-23K18092, KAKENHI-PROJECT-22KJ3188
[Journal Article] Genetic Perturbation Alters Functional Substates in Alkaline Phosphatase2023
- Author(s)
  Sakuma Morito、Honda Shingo、Ueno Hiroshi、Tabata Kazuhito V.、Miyazaki Kentaro、Tokuriki Nobuhiko、Noji Hiroyuki
- Journal Title
  
  Journal of the American Chemical Society
  
  Volume: 145 Issue: 5 Pages: 2806-2814
- DOI
  10.1021/jacs.2c06693
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-19H00936, KAKENHI-PUBLICLY-21H00388
[Journal Article] Direct identification of the rotary angle of ATP cleavage in F1-ATPase from Bacillus PS32023
- Author(s)
  Hasimoto Yuh、Sugawa Mitsuhiro、Nishiguchi Yoshihiro、Aeba Fumihiro、Tagawa Ayari、Suga Kenta、Tanaka Nobukiyo、Ueno Hiroshi、Yamashita Hiroki、Yokota Ryuichi、Masaike Tomoko、Nishizaka Takayuki
- Journal Title
  
  Biophysical Journal
  
  Volume: 122 Issue: 3 Pages: 554-564
- DOI
  10.1016/j.bpj.2022.12.027
- Peer Reviewed
- Data Source
  KAKENHI-PUBLICLY-21H00388, KAKENHI-PROJECT-23K23853
[Journal Article] Molecular mechanism on forcible ejection of ATPase inhibitory factor 1 from mitochondrial ATP synthase2023
- Author(s)
  Kobayashi Ryohei、Ueno Hiroshi、Okazaki Kei-ichi、Noji Hiroyuki
- Journal Title
  
  Nature Communications
  
  Volume: 14 Issue: 1 Pages: 1-12
- DOI
  10.1038/s41467-023-37182-9
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-22KJ3188, KAKENHI-PUBLICLY-21H00388
[Journal Article] Rotary properties of hybrid F1-ATPases consisting of subunits from different species2023
- Author(s)
  Ryo R. Watanabe, Busra Tas Kiper, Mariel Zarco-Zavala, Mayu Hara, Ryohei Kobayashi, Hiroshi Ueno, Jose J. Garcia-Trejo, Chun-Biu Li, Hiroyuki Noji
- Journal Title
  
  iScience
  
  Volume: 26 Issue: 5 Pages: 106626-106626
- DOI
  10.1016/j.isci.2023.106626
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-21J13358, KAKENHI-PUBLICLY-21H00388, KAKENHI-PROJECT-23K18092
[Journal Article] Digital Cascade Assays for ADP- or ATP-Producing Enzymes Using a Femtoliter Reactor Array Device2023
- Author(s)
  Ueno Hiroshi、Sano Mio、Hara Mayu、Noji Hiroyuki
- Journal Title
  
  ACS Sensors
  
  Volume: 8 Issue: 9 Pages: 3400-3407
- DOI
  10.1021/acssensors.3c00587
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-23K18092
[Journal Article] Six states of Enterococcus hirae V-type ATPase reveals non-uniform rotor rotation during turnover2023
- Author(s)
  Burton-Smith Raymond N.、Song Chihong、Ueno Hiroshi、Murata Takeshi、Iino Ryota、Murata Kazuyoshi
- Journal Title
  
  Communications Biology
  
  Volume: 6 Issue: 1 Pages: 755-755
- DOI
  10.1038/s42003-023-05110-8
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-23K18092, KAKENHI-PROJECT-22H04926, KAKENHI-PROJECT-21H02409
[Journal Article] How Does F1-ATPase Generate Torque?: Analysis From Cryo-Electron Microscopy and Rotational Catalysis of Thermophilic F12022
- Author(s)
  Noji Hiroyuki、Ueno Hiroshi
- Journal Title
  
  Frontiers in Microbiology
  
  Volume: 13 Pages: 904084-904084
- DOI
  10.3389/fmicb.2022.904084
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Journal Article] Direct observation of stepping rotation of V-ATPase reveals rigid component in coupling between Vo and V1 motors2022
- Author(s)
  Otomo Akihiro、Iida Tatsuya、Okuni Yasuko、Ueno Hiroshi、Murata Takeshi、Iino Ryota
- Journal Title
  
  Proceedings of the National Academy of Sciences
  
  Volume: 119 Issue: 42
- DOI
  10.1073/pnas.2210204119
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-21K15060, KAKENHI-PLANNED-18H05424, KAKENHI-PUBLICLY-21H00388
[Journal Article] Enzyme-based digital bioassay technology ? key strategies and future perspectives2022
- Author(s)
  Noji Hiroyuki、Minagawa Yoshihiro、Ueno Hiroshi
- Journal Title
  
  Lab on a Chip
  
  Volume: 22 Issue: 17 Pages: 3092-3109
- DOI
  10.1039/d2lc00223j
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Journal Article] Structural Elements Involved in ATP Hydrolysis Inhibition and ATP Synthesis of Tuberculosis and Nontuberculous Mycobacterial F-ATP Synthase Decipher New Targets for Inhibitors2022
- Author(s)
  Wong Chui Fann、Saw Wuan-Geok、Basak Sandip、Sano Mio、Ueno Hiroshi、Kerk Hwee Wen、Litty Dennis、Ragunathan Priya、Dick Thomas、M?ller Volker、Noji Hiroyuki、Gr?ber Gerhard
- Journal Title
  
  Antimicrobial Agents and Chemotherapy
  
  Volume: 66 Issue: 12
- DOI
  10.1128/aac.01056-22
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Journal Article] The six steps of the complete F1-ATPase rotary catalytic cycle2021
- Author(s)
  Sobti Meghna、Ueno Hiroshi、Noji Hiroyuki、Stewart Alastair G.
- Journal Title
  
  Nature Communications
  
  Volume: 12 Issue: 1 Pages: 4690-4690
- DOI
  10.1038/s41467-021-25029-0
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Journal Article] Elucidation and control of low and high active populations of alkaline phosphatase molecules for quantitative digital bioassay2021
- Author(s)
  Ueno Hiroshi、Kato Makoto、Minagawa Yoshihiro、Hirose Yushi、Noji Hiroyuki
- Journal Title
  
  Protein Science
  
  Volume: 30 Issue: 8 Pages: 1628-1639
- DOI
  10.1002/pro.4102
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PUBLICLY-21H00388, KAKENHI-PROJECT-19H05624
[Journal Article] Amplification of Over 100 kbp DNA from Single Template Molecules in Femtoliter Droplets2021
- Author(s)
  Ueno,H., Sawada,H., Soga,N., Sano,M., Nara,S., Tabata,K.V., Su’etsugu, M., Noji,H.
- Journal Title
  
  ACS Synthetic Biology
  
  Volume: 10 Issue: 9 Pages: 2179-2186
- DOI
  10.1021/acssynbio.0c00584
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PLANNED-17H06355, KAKENHI-PUBLICLY-21H00388, KAKENHI-PROJECT-20H03238, KAKENHI-PROJECT-19J20097, KAKENHI-PROJECT-19H05624
[Journal Article] Kinetic analysis of the inhibition mechanism of bovine mitochondrial F1-ATPase inhibitory protein using biochemical assay2021
- Author(s)
  Kobayashi Ryohei、Mori Sougo、Ueno Hiroshi、Noji Hiroyuki
- Journal Title
  
  The Journal of Biochemistry
  
  Volume: － Issue: 1 Pages: 79-87
- DOI
  10.1093/jb/mvab022
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PUBLICLY-19H05380, KAKENHI-PROJECT-19H05624
[Journal Article] Tracking the 3D Rotational Dynamics in Nanoscopic Biological Systems.2020
- Author(s)
  Ryuji Igarashi, Takuma Sugi, Shingo Sotoma, Takuya Genjo, Yuta Kumiya, Erik Walinda, Hiroshi Ueno, Kazuhiro Ikeda, Hitoshi Sumiya, Hidehito Tochio, Yohsuke Yoshinari, Yoshie Harada, Masahiro Shirakawa
- Journal Title
  
  Journal of the American Chemical Society
  
  Volume: 142 Issue: 16 Pages: 7542-7554
- DOI
  10.1021/jacs.0c01191
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18H01838, KAKENHI-PROJECT-20H02587, KAKENHI-PROJECT-18K14665, KAKENHI-PUBLICLY-19H05380
[Journal Article] Tight Chemomechanical Coupling of the F1 Motor Relies on Structural Stability2020
- Author(s)
  Tanaka Mana、Kawakami Tomohiro、Okaniwa Tomoaki、Nakayama Yohei、Toyabe Shoichi、Ueno Hiroshi、Muneyuki Eiro
- Journal Title
  
  Biophysical Journal
  
  Volume: 119 Issue: 1 Pages: 48-54
- DOI
  10.1016/j.bpj.2020.04.039
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19H01864, KAKENHI-PROJECT-18K06164, KAKENHI-PUBLICLY-19H05380
[Journal Article] Rotary catalysis of bovine mitochondrial F1-ATPase studied by single-molecule experiments2020
- Author(s)
  Kobayashi Ryohei、Ueno Hiroshi、Li Chun-Biu、Noji Hiroyuki
- Journal Title
  
  Proceedings of the National Academy of Sciences
  
  Volume: 117 Issue: 3 Pages: 1447-1456
- DOI
  10.1073/pnas.1909407117
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PUBLICLY-19H05380
[Journal Article] The 3 × 120° rotary mechanism ofParacoccus denitrificansF1-ATPase is different from that of the bacterial and mitochondrial F1-ATPases2020
- Author(s)
  Zarco-Zavala Mariel、Watanabe Ryo、McMillan Duncan G. G.、Suzuki Toshiharu、Ueno Hiroshi、Mendoza-Hoffmann Francisco、Garc?a-Trejo Jos? J.、Noji Hiroyuki
- Journal Title
  
  Proceedings of the National Academy of Sciences
  
  Volume: 117 Issue: 47 Pages: 29647-29657
- DOI
  10.1073/pnas.2003163117
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PUBLICLY-19H05380
[Journal Article] Correlation between the numbers of rotation steps in the ATPase and proton-conducting domains of F- and V-ATPases2020
- Author(s)
  Noji Hiroyuki、Ueno Hiroshi、Kobayashi Ryohei
- Journal Title
  
  Biophysical Reviews
  
  Volume: 12 Issue: 2 Pages: 303-307
- DOI
  10.1007/s12551-020-00668-7
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PUBLICLY-19H05380
[Journal Article] Monodisperse Liposomes with Femtoliter Volume Enable Quantitative Digital Bioassays of Membrane Transporters and Cell-Free Gene Expression2020
- Author(s)
  Soga, N., Ota, A., Nakajima, K., Watanabe, R., Ueno, H., & Noji, H.
- Journal Title
  
  ACS Nano
  
  Volume: 14 Issue: 9 Pages: 11700-11711
- DOI
  10.1021/acsnano.0c04354
- Peer Reviewed
- Data Source
  KAKENHI-PLANNED-20H05931, KAKENHI-PUBLICLY-19H05380, KAKENHI-PROJECT-19H05624
[Journal Article] Mobile imaging platform for digital influenza virus counting2019
- Author(s)
  Minagawa Yoshihiro、Ueno Hiroshi、Tabata Kazuhito V.、Noji Hiroyuki
- Journal Title
  
  Lab on a Chip
  
  Volume: 19 Issue: 16 Pages: 2678-2687
- DOI
  10.1039/c9lc00370c
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PUBLICLY-18H04817, KAKENHI-PUBLICLY-19H05380, KAKENHI-PROJECT-19H05624, KAKENHI-PROJECT-19H00849
[Journal Article] Accurate high-throughput screening based on digital protein synthesis in a massively parallel femtoliter droplet array2019
- Author(s)
  Yi Zhang, Yoshihiro Minagawa, Hiroto Kizoe, Kentaro Miyazaki, Ryota Iino, Hiroshi Ueno, Kazuhito V Tabata, Yasuhiro Shimane, Hiroyuki Noji
- Journal Title
  
  Science Advances
  
  Volume: 5 Issue: 8 Pages: 1-11
- DOI
  10.1126/sciadv.aav8185
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18K14260, KAKENHI-PLANNED-17H06355, KAKENHI-PUBLICLY-18H04817, KAKENHI-PUBLICLY-19H05380, KAKENHI-PROJECT-19H05624, KAKENHI-PROJECT-19H00849
[Journal Article] Wash- and Amplification-Free Digital Immunoassay Based on Single-Particle Motion Analysis2019
- Author(s)
  Akama Kenji、Iwanaga Niina、Yamawaki Koya、Okuda Masaki、Jain Krupali、Ueno Hiroshi、Soga Naoki、Minagawa Yoshihiro、Noji Hiroyuki
- Journal Title
  
  ACS Nano
  
  Volume: 13 Issue: 11 Pages: 13116-13126
- DOI
  10.1021/acsnano.9b05917
- Peer Reviewed
- Data Source
  KAKENHI-PUBLICLY-18H04817, KAKENHI-PUBLICLY-19H05380, KAKENHI-PROJECT-19H05624, KAKENHI-PROJECT-19H00849
[Journal Article] Single-molecule analysis reveals rotational substeps and chemo-mechanical coupling scheme of Enterococcus hirae V1-ATPase2019
- Author(s)
  Iida Tatsuya、Minagawa Yoshihiro、Ueno Hiroshi、Kawai Fumihiro、Murata Takeshi、Iino Ryota
- Journal Title
  
  Journal of Biological Chemistry
  
  Volume: 294 Issue: 45 Pages: 17017-17030
- DOI
  10.1074/jbc.ra119.008947
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PLANNED-18H05425, KAKENHI-PUBLICLY-19H05380, KAKENHI-PROJECT-17H03638, KAKENHI-PROJECT-18H02418, KAKENHI-PROJECT-18J23220
[Journal Article] Structure and dynamics of rotary V1 motor2018
- Author(s)
  Ueno Hiroshi、Suzuki Kano、Murata Takeshi
- Journal Title
  
  Cellular and Molecular Life Sciences
  
  Volume: 75 Issue: 10 Pages: 1789-1802
- DOI
  10.1007/s00018-018-2758-3
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-16K14706, KAKENHI-PROJECT-26000011
[Journal Article] Off-axis rotor in Enterococcus hirae V-ATPase visualized by Zernike phase plate single-particle cryo-electron microscopy2018
- Author(s)
  Tsunoda Jun、Song Chihong、Imai Fabiana Lica、Takagi Junichi、Ueno Hiroshi、Murata Takeshi、Iino Ryota、Murata Kazuyoshi
- Journal Title
  
  Scientific Reports
  
  Volume: 8 Issue: 1 Pages: 15632-15632
- DOI
  10.1038/s41598-018-33977-9
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18K19298, KAKENHI-PLANNED-18H05424, KAKENHI-PROJECT-17H01420, KAKENHI-PROJECT-17H03638, KAKENHI-PROJECT-16K14706, KAKENHI-PROJECT-16H06280, KAKENHI-PLANNED-18H05425, KAKENHI-PROJECT-26000011
[Journal Article] Catalytic robustness and torque generation of the F1-ATPase2017
- Author(s)
  Hiroyuki Noji, Hiroshi Ueno and Duncan G. G. McMillan
- Journal Title
  
  Biophysical reviews
  
  Volume: 9 Issue: 2 Pages: 103-103
- DOI
  10.1007/s12551-017-0262-x
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-14F04383, KAKENHI-PROJECT-16K14706, KAKENHI-PROJECT-26000011
[Journal Article] Direct real-time detection of single proteins using silicon nanowire-based electrical circuits2016
- Author(s)
  Jie Li, Gen He, Hiroshi Ueno, Chuancheng Jia, Hiroyuki Noji, Chuanmin Qi, Xuefeng Guo
- Journal Title
  
  Nanoscale
  
  Volume: 8 Issue: 36 Pages: 16172-16176
- DOI
  10.1039/c6nr04103e
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16K14706, KAKENHI-PROJECT-26000011
[Journal Article] Rotation of artificial rotor axles in rotary molecular motors2016
- Author(s)
  Baba M, Iwamoto K, Iino R, Ueno H, Hara M, Nakanishia A, Kishikawa J, Noji H, Yokoyama K
- Journal Title
  
  Proc Natl Acad Sci USA
  
  Volume: 113 Issue: 40 Pages: 11214-11219
- DOI
  10.1073/pnas.1605640113
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PUBLICLY-16H00858, KAKENHI-PROJECT-16K14706, KAKENHI-PROJECT-26000011
[Journal Article] Biophysical Characterization of a Thermoalkaliphilic Molecular Motor with a High Stepping Torque Gives Insight into Evolutionary ATP Synthase Adaptation2016
- Author(s)
  Duncan G. G. McMillan, Rikiya Watanabe, Hiroshi Ueno, Gregory M. Cook, and Hiroyuki Noji
- Journal Title
  
  Journal of Biological Chemistry
  
  Volume: 291 Issue: 46 Pages: 23965-23977
- DOI
  10.1074/jbc.m116.743633
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-14F04383, KAKENHI-PROJECT-16K14706, KAKENHI-PROJECT-26000011
[Journal Article] Rotational mechanism of Enterococcus hirae V1-ATPase by crystal-structure and single-molecule analysis2015
- Author(s)
  Iino R, Minagawa Y, Ueno H, Suzuki K, and Murata T
- Journal Title
  
  Current Opinion in Structural Biology
  
  Volume: 31 Pages: 49-56
- DOI
  10.1016/j.sbi.2015.02.013
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-14J10948, KAKENHI-PROJECT-25840053, KAKENHI-PROJECT-26291009, KAKENHI-PROJECT-15H04366
[Journal Article] Molecular structure and rotary dynamics of Enterococcus hirae V1-ATPase2014
- Author(s)
  Iino R, Minagawa Y, Ueno H, Hara M, and Murata T
- Journal Title
  
  IUBMB Life
  
  Volume: 66 Issue: 9 Pages: 624-630
- DOI
  10.1002/iub.1311
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-14J10948, KAKENHI-PROJECT-25840053, KAKENHI-PROJECT-26291009
[Journal Article] Torque generation of Enterococcus hirae V-ATPase2014
- Author(s)
  Ueno H, Minagawa Y, Hara M, Rahman S, Yamato I, Muneyuki E, Murata T, and Iino R
- Journal Title
  
  The Journal of Biological Chemistry
  
  Volume: 289 Issue: 45 Pages: 31212-31223
- DOI
  10.1074/jbc.m114.598177
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-14J10948, KAKENHI-PLANNED-23115002, KAKENHI-PROJECT-24370062, KAKENHI-PROJECT-24570187, KAKENHI-PROJECT-25251016, KAKENHI-PROJECT-25840053, KAKENHI-PUBLICLY-26103528, KAKENHI-PROJECT-26291009
[Journal Article] Thermodynamic analyses of nucleotide binding to an isolated monomeric β subunit and the α3β3γ subcomplex of F1-ATPase.2013
- Author(s)
  Kikuchi, Y., Naka, Y., Osakabe, H., Okamoto, T., Masaike, T., Ueno, H., Toyabe, S., Muneyuki, E.
- Journal Title
  
  Biophysical Journal
  
  Volume: 105 Issue: 11 Pages: 2541-2548
- DOI
  10.1016/j.bpj.2013.10.018
- Peer Reviewed
- Data Source
  KAKENHI-PUBLICLY-24115517, KAKENHI-PROJECT-24570187, KAKENHI-PUBLICLY-25113524, KAKENHI-PROJECT-25840053
[Journal Article] Catalysis-enhancement via rotary fluctuation of F1-ATPase2013
- Author(s)
  Watanabe R, Hayashi K, Ueno H and Noji H
- Journal Title
  
  Biophys. J
  
  Volume: 105 Issue: 10 Pages: 2385-2391
- DOI
  10.1016/j.bpj.2013.09.050
- Peer Reviewed
- Data Source
  KAKENHI-PLANNED-23115002, KAKENHI-PROJECT-24770143, KAKENHI-PUBLICLY-25117505, KAKENHI-PROJECT-25251016, KAKENHI-PROJECT-25840053
[Journal Article] Common evolutionary origin for the rotor domain of rotary ATPases and Flagellar protein export apparatus.2013
- Author(s)
  Kishikawa, J., et al
- Journal Title
  
  PLoS One
  
  Volume: 8 Issue: 5 Pages: 64692013-64692013
- DOI
  10.1371/journal.pone.0064695
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21227006, KAKENHI-PLANNED-23115008, KAKENHI-PROJECT-23570159, KAKENHI-PROJECT-24370059, KAKENHI-PROJECT-24570131, KAKENHI-PROJECT-25000013, KAKENHI-PUBLICLY-25112507, KAKENHI-PUBLICLY-25121718, KAKENHI-PROJECT-25840053
[Journal Article] Basic properties of rotary dynamics of the molecular motor Enterococcus hirae V1-ATPase2013
- Author(s)
  Yoshihiro Minagawa, Hiroshi Ueno, Mayu Hara, Yoshiko Ishizuka-Katsura, Noboru Ohsawa,Takaho Terada, Mikako Shirouzu, Shigeyuki Yokoyama, Ichiro Yamato, Eiro Muneyuki, Hiroyuki Noji, Takeshi Murata, and Ryota Iino
- Journal Title
  
  J.Biol.Chem
  
  Volume: 288 Issue: 45 Pages: 32700-32707
- DOI
  10.1074/jbc.m113.506329
- Peer Reviewed
- Data Source
  KAKENHI-PLANNED-23115002, KAKENHI-PROJECT-23370047, KAKENHI-PROJECT-24570187, KAKENHI-PROJECT-24651167, KAKENHI-PROJECT-25251016, KAKENHI-PROJECT-25840053
[Patent] 蛍光観察装置2016
- Inventor(s)
  野地博行・上野博史・皆川慶嘉
- Industrial Property Rights Holder
  野地博行・上野博史・皆川慶嘉
- Industrial Property Rights Type
  特許
- Industrial Property Number
  2016-235614
- Filing Date
  2016-12-05
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Single-molecule analysis of the rotation of the common ancestral F1-ATPase of mitochondria or α-proteobacteria2023
- Author(s)
  Hiroki Homma, Hiroshi Ueno, Ryutaro Furukawa, Satoshi Akanuma, Hiroyuki Noji
- Organizer
  第61回日本生物物理学会年会
- Data Source
  KAKENHI-PROJECT-23K18092
[Presentation] Redesigning of F1-ATPase from thermophilic Bacillus PS3 with mitochondrial-specific IF1inhibition sensitivity2023
- Author(s)
  Yuichiro Hatasaki, Ryohei Kobayashi, Ryo Watanabe, Hiroshi Ueno, Hiroyuki Noji
- Organizer
  第61回日本生物物理学会年会
- Data Source
  KAKENHI-PROJECT-23K18092
[Presentation] Resurrection of the Ancestral ATPase2023
- Author(s)
  Aya Suzuki, Ryutaro Furukawa, Hiroshi Ueno, Satoshi Akanuma, Hiroyuki Noji
- Organizer
  Gordon Research Conference (Bioenergetics)
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K18092
[Presentation] Visualization of rotation of F1-ATPase based on scattering imaging of 10 nm nanoparticle2023
- Author(s)
  Keigo Shinoda, Hiroshi Ueno, Hiroyuki Noji
- Organizer
  第61回日本生物物理学会年会
- Data Source
  KAKENHI-PROJECT-23K18092
[Presentation] Development of new DNA recovery technology from fL droplet array using optical tweezers2022
- Author(s)
  Tetsuya Ohashi, Hiroshi Ueno, Yoshihiro Minagawa, Hiroyuki Noji
- Organizer
  第60回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Presentation] In vitro screening of F1 -ATPase based on single molecule rotation assay coupled to cell-free protein synthesis2022
- Author(s)
  Mai Taguchi, Hiroshi Ueno, Hiroyuki Noji
- Organizer
  第60回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Presentation] 全反射型暗視野顕微鏡による生体分子モーターの1分子イメージング2022
- Author(s)
  上野博史
- Organizer
  ニコンWebセミナー
- Invited
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Presentation] ATP driven rotation of mutant FoF1 where H+ translocation is rate-limiting2022
- Author(s)
  Kiyoto Yasuda, Daichi Ando, Ryohei Kobayashi, Hiroshi Ueno, Hiroyuki Noji
- Organizer
  第60回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Presentation] Rotation dynamics and structure of F1-ATPase with all α-subunit-type P-loops2022
- Author(s)
  Hiroshi Ueno, Meghna Sobti, Rie Koga, Tomoko Masaike, Alastair Stewart, Nobuyasu Koga, Hiroyuki Noji
- Organizer
  第60回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Presentation] Resurrection of the Ancestral ATPase2022
- Author(s)
  Aya Suzuki, Ryutaro Furukawa, Hiroshi Ueno, Satoshi Akanuma, Hiroyuki Noji
- Organizer
  第60回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Presentation] Engineering approaches to understanding the design principles of F1-ATPase2022
- Author(s)
  Hiroshi Ueno
- Organizer
  Sendai2022 Workshop
- Invited
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Presentation] Engineering approaches to understanding the unidirectional regulatory function of F1-ATPase2022
- Author(s)
  Hiroshi Ueno
- Organizer
  2022 East Asian Single-Molecule Biophysics Symposium
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Presentation] Torque Generation Mechanism of F1 -ATPase2022
- Author(s)
  Hiroyuki Noji, Hiroshi Ueno
- Organizer
  第60回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Presentation] ) Unidirectional regulation of ATPase factor 1 in mitochondrial ATP synthase studied by single-molecule manipulation experiments2022
- Author(s)
  Ryohei Kobayashi, Hiroshi Ueno, Kei-ichi Okazaki, Hiroyuki Noji
- Organizer
  第60回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Presentation] エンジニアリング的アプローチによる回転分子モーターF1-ATPaseの設計原理の理解2022
- Author(s)
  上野博史
- Organizer
  第11回分子モーター討論会
- Invited
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Presentation] Fluorometric digital ATPase assay with single-enzyme detection sensitivity2021
- Author(s)
  Hiroshi Ueno, Mio Sano, Mayu Hara, Hiroyuki Noji
- Organizer
  第59回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Presentation] Rotary properties of hybrid F1-ATPases consisting of subunits from different species2021
- Author(s)
  Ryo Watanabe, Mariel Zarco-Zavala, Busra Tas, Mayu Hara, Ryohei Kobayashi, Hiroshi Ueno, Jose J. Garcia-Trejo, Chun-Biu Li, Hiroyuki Noji
- Organizer
  第21回日本蛋白質科学会年会
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Presentation] Correlation between the fluctuation of single enzyme activity and evolvability2021
- Author(s)
  Takahito Iida, Hiroshi Ueno, Hiroyuki Noji
- Organizer
  第59回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Presentation] Functional analysis of ATPase complex in Type Three Secretion System of Enteropathogenic Escherichia coli2021
- Author(s)
  Aya Suzuki, Yuki Tajimi, Ryo Kurosaki, Hiroshi Ueno, Takayuki Uchihashi, Hiroyuki Noji
- Organizer
  第59回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Presentation] Cell-freeスクリーニングに向けたF1-ATPaseのin vitro合成及び1分子回転観察系の構築2021
- Author(s)
  田口真衣、上野博史、野地博行
- Organizer
  日本生体エネルギー研究会第47回討論会
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Presentation] ミトコンドリアATP合成酵素における制御因子IF1の一方向反応制御システムを司る分子基盤の探索2021
- Author(s)
  小林稜平、上野博史、野地博行
- Organizer
  日本生体エネルギー研究会第47回討論会
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Presentation] 1分子回転観察と操作によって解明されたミトコンドリア由来ATP合成酵素における阻害因子IF1の制御機構2021
- Author(s)
  小林稜平、上野博史、野地博行
- Organizer
  第21回日本蛋白質科学会年会
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Presentation] Dissociation mechanism of IF1 from mitochondrial ATP synthase revealed by singlemolecule analysis and manipulation2021
- Author(s)
  Ryohei Kobayashi, Hiroshi Ueno, Hiroyuki Noji
- Organizer
  第59回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Presentation] Functional analysis of ATPase complex in Type Three Secretion System of Enteropathogenic Escherichia coli2021
- Author(s)
  Aya Suzuki, Yuki Tajimi, Ryo Kurosaki, Hiroshi Ueno, Takayuki Uchihashi, Hiroyuki Noji
- Organizer
  第21回日本蛋白質科学会年会
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Presentation] Elucidation of molecular recognition mechanism of IF1 , a regulator of F1 -ATPase, studied by an engineering approach2021
- Author(s)
  Yuichiro Hatasaki, Ryo Watanabe, Ryohei Kobayashi, Hiroshi Ueno, Hiroyuki Noji
- Organizer
  第59回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Presentation] In vitro synthesis of F1 -ATPase and construction of single molecule rotation assay system for cell-free screening2021
- Author(s)
  Mai Taguchi, Hiroshi Ueno, Hiroyuki Noji
- Organizer
  第59回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-21H00388
[Presentation] Development of a split enzyme for wash-free digital bioassay2020
- Author(s)
  Yanbo Ma, Hiroshi Ueno, Hiroyuki Noji
- Organizer
  第58回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-19H05380
[Presentation] Elucidation of inhibition mechanism by IF1, a natural inhibitor protein for bovine mitochondrial ATP synthase2020
- Author(s)
  Ryohei Kobayashi, Sougo Mori, Hiroshi Ueno, Hiroyuki Noji
- Organizer
  第58回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-19H05380
[Presentation] Characterization of the enzymatic property and structural dynamics of the T3SS ATPase from Enteropathogenic Escherichia coli2020
- Author(s)
  Aya Suzuki, Hiroshi Ueno, Ryo Kurosaki, Takayuki Uchihashi, Hiroyuki Noji
- Organizer
  第58回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-19H05380
[Presentation] Development of an enzyme-coupled fluorometric digital bioassay for ATPase2020
- Author(s)
  Hiroshi Ueno, Mayu Hara, Mio Sano, Hiroyuki Noji
- Organizer
  第58回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-19H05380
[Presentation] Single-molecule analysis of rotation of Enterococcus hirae V-ATPase depending on sodium ion concentration2020
- Author(s)
  Akihiro Otomo, Tatsuya Iida, Hiroshi Ueno, Takeshi Murata, Ryota Iino
- Organizer
  第58回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-19H05380
[Presentation] Gene expression from a single large DNA encapsulated in artificial cell device2019
- Author(s)
  Yuto Ochiai, Hiroshi Ueno, Masayuki Su'etsugu, Hiroyuki Noji
- Organizer
  第57回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-18H04817
[Presentation] Chemo-mechanical coupling scheme of rotary molecular motor Enterococcus hirae V1-ATPase revealed by single-molecule analysis2019
- Author(s)
  Tatsuya Iida, Yoshihiro Minagawa, Hiroshi Ueno, Fumihiro Kawai, Takeshi Murata, Ryota Iino
- Organizer
  第57回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-19H05380
[Presentation] Digital bioassays and digital gene expression for screening2019
- Author(s)
  Hiroshi Ueno
- Organizer
  The 2nd Conference on Biomotors, Virus Assembly, and Nanobiotechnology Applications
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PUBLICLY-18H04817
[Presentation] Digital bioassays and digital gene expression for screening2019
- Author(s)
  Hiroshi Ueno
- Organizer
  The 2nd Conference on Biomotors, Virus Assembly, and Nanobiotechnology Applications
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PUBLICLY-19H05380
[Presentation] Relationship between fluctuation of single-enzyme activity and evolvability2019
- Author(s)
  Hiroshi Ueno, Morito Sakuma, Yoshihiro Minagawa, Kazuhito Tabata, Kentaro Miyazaki, Hiroyuki Noji
- Organizer
  第57回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-19H05380
[Presentation] Single-molecule analysis of bovine mitochondrial F1-ATPase2019
- Author(s)
  Ryohei Kobayashi, Hiroshi Ueno, Hiroyuki Noji
- Organizer
  第57回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-19H05380
[Presentation] Analysis of the inhibited form of F1-ATPase2019
- Author(s)
  Sougo Mori, Hiroshi Ueno, Hiroyuki Noji
- Organizer
  第57回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-19H05380
[Presentation] Reconstruction and Characterization of Ancestral F1-ATPase2019
- Author(s)
  Nanako Nakama, Hiroshi Ueno, Ryutaro Furukawa, Ryohei Kobayashi, Ryo Watanabe, Satoshi Akanuma, Hiroyuki Noji
- Organizer
  第57回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-19H05380
[Presentation] Gene expression from a single large DNA encapsulated in artificial cell device2019
- Author(s)
  Yuto Ochiai, Hiroshi Ueno, Masayuki Su'etsugu, Hiroyuki Noji
- Organizer
  第57回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-19H05380
[Presentation] Relationship between fluctuation of single-enzyme activity and evolvability2019
- Author(s)
  Hiroshi Ueno, Morito Sakuma, Yoshihiro Minagawa, Kazuhito Tabata, Kentaro Miyazaki, Hiroyuki Noji
- Organizer
  第57回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-18H04817
[Presentation] Rotation observation of hybrid F1-ATPases between bacterial and mammalian ones2019
- Author(s)
  Ryo Watanabe, Hiroshi Ueno, Toshiharu Suzuki, Ryohei Kobayashi, Hiroyuki Noji
- Organizer
  第57回日本生物物理学会年会
- Data Source
  KAKENHI-PUBLICLY-19H05380
[Presentation] Rotational torque and kinetics of F1-ATPase containing the catalytic subunit with a non-catalytic hinge2018
- Author(s)
  Tomoyasu Sato, Hiroshi Ueno, Kumiko Hayashi, Rie Koga, Nobuyasu Koga, Hiroyuki Noji, Tomoko Masaike
- Organizer
  第56回日本生物物理学会年会
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Single Particle Analysis of EhV-ATPase by Phase-Plate electron cryo-microscopy2018
- Author(s)
  Jun Tsunoda, Chihong Song, Yakushiji Fabiana Lica, Takeshi Murata, Hiroshi Ueno, Naoyuki Miyazaki, Kenji Iwasaki, Junichi Takagi, Ryota Iino, Kazuyoshi Murata
- Organizer
  第56回日本生物物理学会年会
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Rotation of the engineered F1-ATPase with non-catalytic α-type P-loops2018
- Author(s)
  Hiroshi Ueno, Rie Koga, Tomoko Masaike, Nobuyasu Koga, Hiroyuki Noji
- Organizer
  20th European Bioenergetics Conference
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Evaluation of correlation between fluctuation of enzyme activity and evolvability by single enzyme activity measurement2018
- Author(s)
  佐久間守仁, 上野博史, 宮崎健太郎, 田端和仁, 野地博行
- Organizer
  日本生物物理学会第56回年会
- Data Source
  KAKENHI-PUBLICLY-18H04817
[Presentation] On the timing of phosphate release in the ATPase reaction by TF12018
- Author(s)
  Eiro Muneyuki, Yohei Nakayama, Shoichi Toyabe, Hiroshi Ueno
- Organizer
  第56回日本生物物理学会年会
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Rotation of Fusobacterium nucleatum F1-ATPase2018
- Author(s)
  Hiroshi Ueno
- Organizer
  Bilateral joint research project meeting in Otago
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Rotation of hybrid F1-ATPases between bacterial and mammalian ones2018
- Author(s)
  Ryo Watanabe, Hiroshi Ueno, Toshiharu Suzuki, Ryohei Kobayashi, Hiroyuki Noji
- Organizer
  第56回日本生物物理学会年会
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Chemomechanical Coupling of the Paracoccus denitrificans F1-ATPase2018
- Author(s)
  Mariel Zarco - Zavala, Duncan G.G McMillan, Suzuki Toshiharu, Hiroshi Ueno, Rikiya Watanabe, Francisco Mendoza - Hoffmann, Jose J. Garcia-Trejo, Hiroyuki Noji
- Organizer
  第56回日本生物物理学会年会
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Rotation of the engineered F1-ATPase with non-catalytic α-type P-loops2018
- Author(s)
  Hiroshi Ueno, Rie Koga, Tomoko Masaike, Nobuyasu Koga, Hiroyuki Noji
- Organizer
  第56回日本生物物理学会年会
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Rotation mechanisms of rotary ATPases2018
- Author(s)
  Hiroshi Ueno
- Organizer
  Complex systems and Biological physics seminar
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Single-molecule analysis of bovine mitochondrial F1-ATPase for direct assignment of crystal structures and rotational pausing states2018
- Author(s)
  Ryohei Kobayashi, Hiroshi Ueno, Toshiharu Suzuki, Mayu Hara, Hiroyuki Noji
- Organizer
  第56回日本生物物理学会年会
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Impact of the sequence difference of P-loop on the conformational changes of F1-ATPase2018
- Author(s)
  Rie Koga, Hiroshi Ueno, Tomoko Masaike, Hiroyuki Noji, Nobuyasu Koga
- Organizer
  第56回日本生物物理学会年会
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Screening and Measurement of Mutated Alkaline Phosphatase by a Super-Arrayed Reactor2018
- Author(s)
  Morito Sakuma, Hiroshi Ueno, Yoshiki Minagawa, Kentaro Miyazaki, Kazuhito Tabata, Hiroyuki Noji
- Organizer
  Protein Engineering Canada Conference 2018
- Int'l Joint Research
- Data Source
  KAKENHI-PUBLICLY-18H04817
[Presentation] Single-molecule imaging of rotation of bovine mitochondrial F1-ATPase2017
- Author(s)
  Hiroshi Ueno
- Organizer
  Gordon Research Conference, Bioenergetics
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Rotation of the engineered F1-ATPase with alpha-type P-loop on catalytic beta subunit2017
- Author(s)
  Hiroshi Ueno
- Organizer
  第55回日本生物物理学会年会
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Single-molecule simultaneous observation of rotation and nucleotide binding/release of Enterococcus hirae V1-ATPase2016
- Author(s)
  Yoshihiro Minagawa, Hiroshi Ueno, Hiroyuki Noji, Takeshi Murata, Ryota Iino
- Organizer
  第54回日本生物物理学会年会
- Place of Presentation
  つくば国際会議場（茨城県つくば市）
- Year and Date
  2016-11-26
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Probing the biophysical properties of a Thermoalkaliphilic F1 ATPase gives insight into adaptation and regulation2016
- Author(s)
  Duncan G. G. McMillan, Rikiya Watanabe, Hiroshi Ueno, Gregory M. Cook, Hiroyuki Noji
- Organizer
  第54回日本生物物理学会年会
- Place of Presentation
  つくば国際会議場（茨城県つくば市）
- Year and Date
  2016-11-27
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Single Particle Analysis of EhV-ATPase by Phase-contrast cryo-Electron Microscopy2016
- Author(s)
  Jun Tsunoda, Chihong Song, Fabiana Lica Yakushiji, Takeshi Murata, Hiroshi Ueno, Junichi Takagi, Ryota Iino, Kazuyoshi Murata
- Organizer
  第54回日本生物物理学会年会
- Place of Presentation
  つくば国際会議場（茨城県つくば市）
- Year and Date
  2016-11-27
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] The kinetics of Pi release in F1-ATPase investigated with P-loop mutations2016
- Author(s)
  Hiroka Narita, Hitoshi Hoshina, Hikaru Yoshida, Yohei Nakayama, Shoichi Toyabe, Hiroshi Ueno, Eiro Muneyuki
- Organizer
  第54回日本生物物理学会年会
- Place of Presentation
  つくば国際会議場（茨城県つくば市）
- Year and Date
  2016-11-25
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Measuring metabolic activities in single bacterial cells by Raman microspectroscopy2016
- Author(s)
  Yota Kato, Hiroshi Ueno, Hiroyuki Noji
- Organizer
  International Conference on Single Cell Research 2016
- Place of Presentation
  Ito International Research Center, The University of Tokyo（東京都文京区）
- Year and Date
  2016-11-17
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Detection of enzymatic activity in femtliter droplets using micro-Raman spectroscopy2016
- Author(s)
  Hironobu Yamashita, Kazuhito V Tabata, Hiroshi Ueno, Hiroyuki Noji
- Organizer
  第54回日本生物物理学会年会
- Place of Presentation
  つくば国際会議場（茨城県つくば市）
- Year and Date
  2016-11-26
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Unveiling the chemomechanical coupling of F1 ATPase of Paracoccus denitrificans2016
- Author(s)
  Mariel Zarco Zavala, Duncan G G McMillan, Toshiharu Suzuki, Hiroshi Ueno, Francisco Mendoza-Hoffmann, Jose J. Garcia-Trejo, Hiroyuki Noji
- Organizer
  第54回日本生物物理学会年会
- Place of Presentation
  つくば国際会議場（茨城県つくば市）
- Year and Date
  2016-11-27
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Measuring metabolic activities in single bacterial cells by Raman microspectroscopy2016
- Author(s)
  Yota Kato, Hiroshi Ueno, Hiroyuki Noji
- Organizer
  第54回日本生物物理学会年会
- Place of Presentation
  つくば国際会議場（茨城県つくば市）
- Year and Date
  2016-11-26
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Fluorescence detection of the flagellar type III protein export using the inverted membrane vesicles2016
- Author(s)
  Tsuyoshi Tono, Hiroyuki Terashima, Kazuhito Tabata, Hiroshi Ueno, Tomoki Matsuda, Takeharu Nagai, Hiroyuki Noji, Katsumi Imada
- Organizer
  第54回日本生物物理学会年会
- Place of Presentation
  つくば国際会議場（茨城県つくば市）
- Year and Date
  2016-11-27
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Identification of accelerators on Na+-depending ATPase activity of Enterococcus hirae V-ATPase2016
- Author(s)
  Senka Gi, Lica Fabiana Yakushiji, Hiroshi Ueno, Hiroyuki Noji, Takayoshi Arai, Katsuhiko Moriyama, Hideo Togo, Takeshi Murata
- Organizer
  第54回日本生物物理学会年会
- Place of Presentation
  つくば国際会議場（茨城県つくば市）
- Year and Date
  2016-11-25
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] 金ナノ粒子を用いた無負荷でのV1-ATPaseの回転とヌクレオチド結合解離の同時計測2016
- Author(s)
  上野博史
- Organizer
  蛋白研セミナー・第６回分子モーター討論会
- Place of Presentation
  大阪大学蛋白質研究所（大阪府吹田市）
- Year and Date
  2016-07-25
- Invited
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Arginine finger mutant of Enterococcus hirae V1-ATPase shows unusual rotational behaviors2016
- Author(s)
  Tatsuya Iida, Yoshihiro Minagawa, Hiroshi Ueno, Takeshi Murata, Ryota Iino
- Organizer
  第54回日本生物物理学会年会
- Place of Presentation
  つくば国際会議場（茨城県つくば市）
- Year and Date
  2016-11-27
- Data Source
  KAKENHI-PROJECT-16K14706
[Presentation] Comparison of the nucleotide binding to the isolated β subunit and the F1-ATPase using the Sopped-Flow method2013
- Author(s)
  長埜陸, 小原清志, 政池知子, 上野博史, 宗行英朗
- Organizer
  第51回日本生物物理学会年会
- Place of Presentation
  国立京都国際会館
- Data Source
  KAKENHI-PROJECT-25840053
[Presentation] The relationship between F1-ATPase P-loop mutants and Pi release2013
- Author(s)
  吉田光, 伊藤亜由美, 伊藤丈太郎, ,政池知子, 西坂崇之, 鳥谷部祥一, 上野博史, 宗行英朗
- Organizer
  第51回日本生物物理学会年会
- Place of Presentation
  国立京都国際会館
- Data Source
  KAKENHI-PROJECT-25840053
[Presentation] Basic properties of rotary dynamics of Enterococcus hirae V1-ATPAse motor protein2013
- Author(s)
  皆川慶嘉, 上野博史, 石塚芳子, 大沢登, 寺田貴帆, 白水美香子, 横山茂之, 野地博行, 村田武士, 飯野亮太
- Organizer
  第51回日本生物物理学会年会
- Place of Presentation
  国立京都国際会館
- Data Source
  KAKENHI-PROJECT-25840053
[Presentation] Direct observation of the rotation of V1-ATPase from Enterococcus hirae and its torque2013
- Author(s)
  上野博史, 皆川慶嘉, 山登一郎, 村田武志, 飯野亮太, 宗行英朗
- Organizer
  第51回日本生物物理学会年会
- Place of Presentation
  国立京都国際会館
- Data Source
  KAKENHI-PROJECT-25840053
[Presentation] Single-molecule nonequilibrium thermodynamics of molecular motor2011
- Author(s)
  S.Toyabe, H.Ueno, E.Muneyuki
- Organizer
  第49回日本生物物理学会年会
- Place of Presentation
  兵庫県立大学姫路書写キャンパス(招待講演)
- Year and Date
  2011-09-16
- Data Source
  KAKENHI-PROJECT-23654137
[Presentation] The role of amino acid residues located at the catalytic site in the rotation of Enterococcus hirae V1-ATPase
- Author(s)
  皆川慶嘉、上野博史、原舞雪、野地博行、村田武士、飯野亮太
- Organizer
  第52回日本生物物理学会
- Place of Presentation
  札幌コンベンションセンター
- Year and Date
  2014-09-25 – 2014-09-27
- Data Source
  KAKENHI-PROJECT-25840053
[Presentation] 腸内連鎖球菌由来のV-ATPaseの1分子回転計測
- Author(s)
  上野博史、皆川慶嘉、宗行英朗、野地博行、村田武士、飯野亮太
- Organizer
  分子研研究会
- Place of Presentation
  岡崎コンファレンスセンター
- Year and Date
  2015-04-20 – 2015-04-21
- Data Source
  KAKENHI-PROJECT-25840053
[Presentation] Rotor-Stator Interactions in V1 and Vo from Enterococcus hirae V-ATPase
- Author(s)
  上野博史、皆川慶嘉、原舞雪、山登一郎、野地博行、村田武士、飯野亮太、宗行英朗
- Organizer
  第52回日本生物物理学会
- Place of Presentation
  札幌コンベンションセンター
- Year and Date
  2014-09-25 – 2014-09-27
- Data Source
  KAKENHI-PROJECT-25840053
[Presentation] Interactions between Rotor and Stator in Enterococcus hirae V-ATPase
- Author(s)
  Ueno H, Minagawa Y, Hara M, Noji H, Murata T, Iino R, Muneyuki E
- Organizer
  Tokyo ATPase Workshop
- Place of Presentation
  東京大学弥生キャンパス武田ホール
- Year and Date
  2014-06-02 – 2014-06-03
- Data Source
  KAKENHI-PROJECT-25840053
[Presentation] Basic properties of rotary dynamics of the molecular motor Enterococcus hirae V1-ATPase
- Author(s)
  Minagawa Y, Ueno H, Hara M, Noji H, Murata T, Iino R
- Organizer
  Tokyo ATPase Workshop
- Place of Presentation
  東京大学弥生キャンパス武田ホール
- Year and Date
  2014-06-02 – 2014-06-03
- Data Source
  KAKENHI-PROJECT-25840053
[Presentation] ナトリウムを輸送するV-ATPaseの1分子回転観察
- Author(s)
  上野博史
- Organizer
  第6回膜輸送体研究会
- Place of Presentation
  ニュー富良野ホテル
- Year and Date
  2014-12-26 – 2014-12-28
- Data Source
  KAKENHI-PROJECT-25840053

1. 鳥谷部祥一 (40453675)

# of Collaborated Projects: 1 results

# of Collaborated Products: 1 results
2. 今田勝巳 (40346143)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
3. MURATA takeshi

# of Collaborated Projects: 1 results

# of Collaborated Products: 7 results
4. NOJI hiroyuki

# of Collaborated Projects: 1 results

# of Collaborated Products: 20 results
5. MIYAZAKI Kentaro

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
6. MASAIKE Tomoko

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
7. TABATA Kazuhito

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
8. IINO Ryota

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
9. 原田慶恵

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
10. 中山洋平

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
11. 張翼

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
12. 高木淳一

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
13. 大友章裕

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
14. 渡邉朋信

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
15. 渡邉力也

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
16. MCMILLAN Duncan

# of Collaborated Projects: 0 results

# of Collaborated Products: 6 results

UENO HIROSHI 上野 博史

Ueno Hiroshi 上野 博史

UENO Hiroshi 上野 博史

上野 博史 ウエノ ヒロシ

Research Projects

Research Products

Co-Researchers

Creation of ATP synthase with multiple Fo torque generating unitsPrincipal Investigator

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Elucidation of the evolution of the rotary catalysis mechanism of F1-ATPase by reconstruction of the ancestral enzymePrincipal Investigator

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固定子イオンチャネル型モーターの作動機構の解明

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新規on-chipスクリーニング技術による分子機械の機能改変Principal Investigator

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生体分子の改造・創成を実現する新規進化分子工学的スクリーニング技術の開発Principal Investigator

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酵素１分子の活性揺らぎと進化能の関係Principal Investigator

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Elucidation of rotary ion transport mechanism of V-ATPase by visualization and control of substratePrincipal Investigator

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Elucidation of operation mechanism of rotary molecular motors by comparison with the mechanisms of F1 and V1-ATPases.Principal Investigator

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分子モーターの1分子トラジェクトリからポテンシャルを描く

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[Book] FoF1モーター 「DOJIN BIOSCIENCE SERIES 17 1分子生物学」2014

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[Journal Article] The series of conformational states adopted by rotorless F1-ATPase during its hydrolysis cycle2024

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[Journal Article] Engineering of IF<sub>1</sub>‐susceptive bacterial F<sub>1</sub>‐ATPase2024

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[Journal Article] Genetic Perturbation Alters Functional Substates in Alkaline Phosphatase2023

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UENO HIROSHI 上野博史

Ueno Hiroshi 上野博史

UENO Hiroshi 上野博史

上野博史ウエノヒロシ

[Book] FoF1モーター　「DOJIN BIOSCIENCE SERIES 17　1分子生物学」2014