Sakaguchi Yohei 坂口洋平

Researcher Number	10712507
Other IDs
Affiliation (based on the past Project Information) *help	2021 – 2023: 福岡大学, 薬学部, 助教 2018: 福岡大学, 薬学部, 助教 2016 – 2017: 国立研究開発法人産業技術総合研究所, 物質計測標準研究部門, 研究員 2015: 国立研究開発法人産業技術総合研究所, 物質計測標準部門, 研究員
Review Section/Research Field	Principal Investigator Basic Section 34020:Analytical chemistry-related / Analytical chemistry Except Principal Investigator Basic Section 34020:Analytical chemistry-related
Keywords	Principal Investigator 誘導体化 / LC-MS / 生体試料分析 / キラル誘導体化 / LC / フルオラスキラル誘導体化 / カルボキシル基 / アミノ酸 / ペプチド / タンパク質 … More Except Principal Investigator … More 蛍光誘導体化LC分析 / 液体クロマトグラフィー / オンライン還元検出 / ジスルフィド試薬 / 消光性物質 / 蛍光誘導体化 Less

試料中の夾雑成分との分離に特化したキラル誘導体化LCの開発Principal Investigator
- Principal Investigator
  
  坂口洋平
- Project Period (FY)
  2022
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 34020:Analytical chemistry-related
- Research Institution
  Fukuoka University
オンライン還元反応を用いた測定対象物の構造に依存しない蛍光誘導体化LC定量法
- Principal Investigator
  
  能田均
- Project Period (FY)
  2021 – 2025
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 34020:Analytical chemistry-related
- Research Institution
  Fukuoka University
Development of sensitive and selective LC-MS method for protein and assessment method for deamidation of protein using carboxy group derivatizationPrincipal Investigator
- Principal Investigator
  
  Sakaguchi Yohei
- Project Period (FY)
  2015 – 2018
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Analytical chemistry
- Research Institution
  Fukuoka University
  National Institute of Advanced Industrial Science and Technology

All 2022 2021 2018 2017 2016 2015

All Journal Article Presentation

[Journal Article] A Pyridinium Derivatization Reagent for Highly Sensitive Detection of Poly(carboxylic acid)s Using Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry2022
- Author(s)
  Kawasue Shimba、Sakaguchi Yohei、Koga Reiko、Yoshida Hideyuki、Nohta Hitoshi
- Journal Title
  
  Journal of the American Society for Mass Spectrometry
  
  Volume: 33 Issue: 8 Pages: 1492-1498
- DOI
  10.1021/jasms.2c00086
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19K05920, KAKENHI-PROJECT-21K05135
[Journal Article] Quantification of Casein in Baked Food Products by Selective Analysis of Phosphorylated Peptides Using Fluorous Derivatization with Liquid Chromatography-Tandem Mass Spectrometry Method2022
- Author(s)
  Kawasue Shimba、Sakaguchi Yohei、Koga Reiko、Hayama Tadashi、Yoshida Hideyuki、Nohta Hitoshi
- Journal Title
  
  Chemical and Pharmaceutical Bulletin
  
  Volume: 70 Issue: 1 Pages: 19-24
- DOI
  10.1248/cpb.c21-00650
- NAID
  130008138707
- ISSN
  0009-2363, 1347-5223
- Year and Date
  2022-01-01
- Language
  English
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-19K05920, KAKENHI-PROJECT-21K05135
[Journal Article] Enantiomeric separation of chiral amines in biological samples by liquid chromatography with chiral fluorous derivatization2022
- Author(s)
  Sakaguchi Yohei、Yamada Hajime、Ishibashi Yukiko、Nakatake Itsuki、Kawasue Shimba、Koga Reiko、Yoshida Hideyuki、Nohta Hitoshi
- Journal Title
  
  SEPARATION SCIENCE PLUS
  
  Volume: 5 Issue: 10 Pages: 559-565
- DOI
  10.1002/sscp.202200069
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19K05920, KAKENHI-PROJECT-21K05135, KAKENHI-PROJECT-22K05161
[Journal Article] Selective analysis of the okadaic acid group in shellfish samples using fluorous derivatization coupled with liquid chromatography-tandem mass spectrometry2021
- Author(s)
  Sakaguchi Yohei、Kawamura Rina、Nakayama Erina、Ako Kenta、Kawasue Shimba、Koga Reiko、Yoshida Hideyuki、Nohta Hitoshi
- Journal Title
  
  Journal of Chromatography B
  
  Volume: 1173 Pages: 122681-122681
- DOI
  10.1016/j.jchromb.2021.122681
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19K05920, KAKENHI-PROJECT-21K05135
[Journal Article] Multiple phosphorylated protein selective analysis via fluorous derivatization and liquid chromatography-electrospray ionization-mass spectrometry analysis2021
- Author(s)
  Kawasue Shimba、Sakaguchi Yohei、Koga Reiko、Hayama Tadashi、Yoshida Hideyuki、Nohta Hitoshi
- Journal Title
  
  Analytical Biochemistry
  
  Volume: 628 Pages: 114247-114247
- DOI
  10.1016/j.ab.2021.114247
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19K05920, KAKENHI-PROJECT-21K05135, KAKENHI-PROJECT-21K06507
[Journal Article] Isotope-dilution liquid chromatography-tandem mass spectrometry for sensitive quantification of human insulin in serum using derivatization-technique2017
- Author(s)
  Sakaguchi Yohei、Kinumi Tomoya、Takatsu Akiko
- Journal Title
  
  Analytical Biochemistry
  
  Volume: 537 Pages: 26-32
- DOI
  10.1016/j.ab.2017.08.019
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K21662
[Journal Article] Quantification of peptides using N-terminal isotope coding and C-terminal derivatization for sensitive analysis by micro liquid chromatography-tandem mass spectrometry.2016
- Author(s)
  Yohei SAKAGUCHI, Tomoya KINUMI, Akiko TAKATSU
- Journal Title
  
  Journal of mass spectrometry
  
  Volume: 51 Issue: 12 Pages: 1111-1119
- DOI
  10.1002/jms.3845
- Peer Reviewed / Acknowledgement Compliant / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K21662
[Journal Article] Quantification of glycated N-terminal peptide of hemoglobin using derivatization for multiple functional groups of amino acids followed by liquid chromatography/tandem mass spectrometry.2016
- Author(s)
  Sakaguchi Y, Kinumi T, Yamazaki T, Takatsu A.
- Journal Title
  
  Biomed Chromatogr
  
  Volume: 30 Issue: 2 Pages: 280-284
- DOI
  10.1002/bmc.3524
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-15K21662
[Journal Article] Selective and sensitive liquid chromatographic determination method of 5-hydroxyindoles with fluorous and fluorogenic derivatization2015
- Author(s)
  Yohei Sakaguchi, Jun Ikenaga, Hideyuki Yoshida, Tadashi Hayama, Miki Itoyama, Kenichiro Todoroki, Osamu Imakyure, Masatoshi Yamaguchi, Hitoshi Nohta
- Journal Title
  
  Journal of Pharmaceutical and Biomedical Analysis
  
  Volume: 114 Pages: 348-354
- DOI
  10.1016/j.jpba.2015.06.003
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-15K07911, KAKENHI-PROJECT-26410166, KAKENHI-PROJECT-26460182, KAKENHI-PROJECT-15K21662, KAKENHI-PROJECT-25810090, KAKENHI-PROJECT-24619009, KAKENHI-PROJECT-25870994
[Journal Article] 二官能基誘導体化とマイクロＬＣ－ＭＳ／ＭＳによる生理活性ペプチド類の高感度分析2015
- Author(s)
  坂口洋平、絹見朋也、高津章子
- Journal Title
  
  Chromatography
  
  Volume: 36 Pages: 52-52
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] 二次元キラルHPLCを用いるカルノシンの高選択的分析法の開発2022
- Author(s)
  守田羽吟，川末慎葉，古賀鈴依子，坂口洋平，三田真史，浜瀬健司，吉田秀幸，能田均
- Organizer
  日本薬学会第142年会
- Data Source
  KAKENHI-PROJECT-21K05135
[Presentation] Arg-C様トリプシン消化による低アレルゲン性調製粉乳中のミルクアレルゲンLC-MS分析2022
- Author(s)
  江頭順子，川末慎葉，坂口洋平，古賀鈴依子，吉田秀幸，能田均
- Organizer
  日本薬学会第142年会
- Data Source
  KAKENHI-PROJECT-21K05135
[Presentation] 酸加水分解-誘導体化-LC-MS法によるタンパク質のアシル化度評価法2022
- Author(s)
  川末慎葉，坂口洋平，古賀鈴依子，吉田秀幸，能田均
- Organizer
  日本薬学会第142年会
- Data Source
  KAKENHI-PROJECT-21K05135
[Presentation] フルオラス誘導体化-LC-MS/MS法による食品および食用油中アクリルアミドの高選択的定量2022
- Author(s)
  重松孝尚，川末慎葉，坂口洋平，古賀鈴依子，吉田秀幸，能田均
- Organizer
  日本薬学会第142年会
- Data Source
  KAKENHI-PROJECT-21K05135
[Presentation] Highly selective 2D-HPLC analysis of ornithine and lysine enantiomers in human physiological fluids based on intramolecular excimer-forming derivatization2021
- Author(s)
  Reiko Koga, Nao Kimura, Yoshiki Sumi, Yohei Sakaguchi, Masashi Mita, Tomomi Ide, Kenji Hamase, Hideyuki Yoshida, Hitoshi Nohta
- Organizer
  The International Chemical Congress of Pacific Basin Societies 2021 (Pacifichem 2021)
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-21K05135
[Presentation] タンパク質加水分解アミノ酸を対象としたフルオラスキラル誘導体化LC-MS分析2021
- Author(s)
  中武樹, 坂口洋平，古賀鈴依子，吉田秀幸，能田均
- Organizer
  第32回クロマトグラフィー科学会議
- Data Source
  KAKENHI-PROJECT-21K05135
[Presentation] 三次元キラルHPLCを用いるリジンおよび代謝物の生体内含量解析2021
- Author(s)
  古賀鈴依子，荒牧雄也，三田真史，井手友美，坂口洋平，吉田秀幸，能田均，浜瀬健司
- Organizer
  第33回バイオメディカル分析科学シンポジウム
- Data Source
  KAKENHI-PROJECT-21K05135
[Presentation] フルオラス誘導体化LC-MS/MS法を用いた生体試料中シアル酸関連化合物の選択的分析2021
- Author(s)
  坂口洋平, 川末慎葉，古賀鈴依子，巴山忠，吉田秀幸，能田均
- Organizer
  第69回質量分析総合討論会
- Data Source
  KAKENHI-PROJECT-21K05135
[Presentation] フルオラス誘導体化-LC-MS 法を用いた食品中カゼインの選択的分析2021
- Author(s)
  川末慎葉，坂口洋平，古賀鈴依子，巴山忠，吉田秀幸，能田均
- Organizer
  第18回次世代を担う若手のためのフィジカル・ファーマフォーラム（PPF2020/2021）
- Data Source
  KAKENHI-PROJECT-21K05135
[Presentation] 蛍光誘導体化HPLC法による二枚貝中オカダ酸の分析2018
- Author(s)
  吉田秀幸，河村梨那，中山絵梨奈，古賀鈴依子，矢野（清川）恵奈，坂口洋平，山口政俊，能田均
- Organizer
  日本分析化学会第67年会
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] ヒト血漿中クルクミンのフルオラス誘導体化LC-MS/MS分析2018
- Author(s)
  濱岡祐司，矢野恵奈，巴山忠，古賀鈴依子，坂口洋平，吉田秀幸，能田均
- Organizer
  第31回バイオメディカル分析科学シンポジウム
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] カルボキシル基誘導体化LC-MS法によるタンパク質の脱アミド化評価法の開発2018
- Author(s)
  川末慎葉，坂口洋平，古賀鈴依子，吉田秀幸，能田均
- Organizer
  日本薬学会第139年会
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] キヌレニン鏡像異性体を対象とした高選択的二次元HPLC分析法の開発2018
- Author(s)
  甲斐永美里，古賀鈴依子，坂口洋平，三田真史，井手友美，吉田秀幸，能田均，濵瀬健司
- Organizer
  第36回九州分析化学若手の会夏季セミナー
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] 同位体希釈質量分析法を用いたヒトインスリン認証標準物質の開発2018
- Author(s)
  坂口洋平
- Organizer
  第31回九州分析化学若手の会春の講演会
- Invited
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] フルオラス誘導体化LC-MS法による二枚貝中のオカダ酸分析2018
- Author(s)
  赤穂　健太、坂口　洋平、河村　梨那、吉田　秀幸、古賀　鈴依子、能田　均
- Organizer
  フルオラス科学研究会第11回シンポジウム
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] フルオラス誘導体化LC-MS法による貝毒成分のオカダ酸及びドウモイ酸の分析2018
- Author(s)
  赤穂健太，吉田秀幸，坂口洋平，古賀鈴依子，能田均
- Organizer
  第36回九州分析化学若手の会夏季セミナー
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] パーフルオロポリエーテルカルボン酸の金属塩を利用したリン脂質の選択的抽出及びLC-MS/MS分析2018
- Author(s)
  田坂友里恵，矢野恵奈，巴山忠，古賀鈴依子，坂口洋平，吉田秀幸，能田均
- Organizer
  第31回バイオメディカル分析科学シンポジウム
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] 多次元HPLCを用いる代謝関連キラルアミノ酸の高選択的分析法開発2018
- Author(s)
  古賀鈴依子，甲斐永美里，篠島朋子，三田真史，井手友美，坂口洋平，吉田秀幸，能田均，濵瀬健司
- Organizer
  第14回D-アミノ酸学会学術講演会
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] Development of a carboxyl group derivatization LC method for monitoring the deamidation of protein2017
- Author(s)
  Yohei Sakaguchi, Tomoya Kinumi, Akiko Takatsu
- Organizer
  HPLC2017
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] A dual functional-group derivatization liquid chromatography-tandem mass spectrometry method; application for quantification of human insulin using enzymatic digestion2016
- Author(s)
  Yohei SAKAGUCHI, Tomoya KINUMI, Akiko TAKATSU
- Organizer
  HPLC 2016, 44th International Symposium on High Performance Liquid Phase Separations and Related Techniques
- Place of Presentation
  San Francisco Marriott Marquis、サンフランシスコ、アメリカ
- Year and Date
  2016-06-19
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] カルボキシル基誘導体化LCを用いたタンパク質脱アミド化の評価法の開発2016
- Author(s)
  坂口洋平、絹見朋也、高津章子
- Organizer
  日本分析化学会第65年会
- Place of Presentation
  北海道大学、札幌市
- Year and Date
  2016-09-14
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] 二官能基誘導体化-ＬＣ－ＭＳ／ＭＳによる生理活性ペプチド類の高感度分析2015
- Author(s)
  坂口洋平、絹見朋也、高津章子
- Organizer
  第63回質量分析総合討論会
- Place of Presentation
  つくば市
- Year and Date
  2015-06-17
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] 二官能基誘導体化とマイクロＬＣ－ＭＳ／ＭＳによる生理活性ペプチド類の高感度分析2015
- Author(s)
  坂口洋平、絹見朋也、高津章子
- Organizer
  第26回クロマトグラフィー科学会議
- Place of Presentation
  福岡市
- Year and Date
  2015-11-11
- Data Source
  KAKENHI-PROJECT-15K21662

1. 能田均 (20164668)

# of Collaborated Projects: 1 results

# of Collaborated Products: 9 results
2. Hayama Tadashi

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
3. 吉田秀幸

# of Collaborated Projects: 0 results

# of Collaborated Products: 4 results
4. 山口政俊

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results

Sakaguchi Yohei 坂口 洋平

Research Projects

Research Products

Co-Researchers

試料中の夾雑成分との分離に特化したキラル誘導体化LCの開発Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

オンライン還元反応を用いた測定対象物の構造に依存しない蛍光誘導体化LC定量法

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Development of sensitive and selective LC-MS method for protein and assessment method for deamidation of protein using carboxy group derivatizationPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

[Journal Article] A Pyridinium Derivatization Reagent for Highly Sensitive Detection of Poly(carboxylic acid)s Using Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry2022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Quantification of Casein in Baked Food Products by Selective Analysis of Phosphorylated Peptides Using Fluorous Derivatization with Liquid Chromatography-Tandem Mass Spectrometry Method2022

Author(s)

Journal Title

DOI

NAID

ISSN

Year and Date

Language

Data Source

[Journal Article] Enantiomeric separation of chiral amines in biological samples by liquid chromatography with chiral fluorous derivatization2022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Selective analysis of the okadaic acid group in shellfish samples using fluorous derivatization coupled with liquid chromatography-tandem mass spectrometry2021

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Multiple phosphorylated protein selective analysis via fluorous derivatization and liquid chromatography-electrospray ionization-mass spectrometry analysis2021

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Isotope-dilution liquid chromatography-tandem mass spectrometry for sensitive quantification of human insulin in serum using derivatization-technique2017

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Quantification of peptides using N-terminal isotope coding and C-terminal derivatization for sensitive analysis by micro liquid chromatography-tandem mass spectrometry.2016

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Quantification of glycated N-terminal peptide of hemoglobin using derivatization for multiple functional groups of amino acids followed by liquid chromatography/tandem mass spectrometry.2016

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Selective and sensitive liquid chromatographic determination method of 5-hydroxyindoles with fluorous and fluorogenic derivatization2015

Author(s)

Journal Title

DOI

Data Source

[Journal Article] 二官能基誘導体化とマイクロＬＣ－ＭＳ／ＭＳによる生理活性ペプチド類の高感度分析2015

Author(s)

Journal Title

Data Source

[Presentation] 二次元キラルHPLCを用いるカルノシンの高選択的分析法の開発2022

Author(s)

Organizer

Data Source

[Presentation] Arg-C様トリプシン消化による低アレルゲン性調製粉乳中のミルクアレルゲンLC-MS分析2022

Sakaguchi Yohei 坂口洋平