Sakaguchi Yohei 坂口洋平

Researcher Number	10712507
Other IDs
Affiliation (Current)	2021: 福岡大学, 薬学部, 助教
Affiliation (based on the past Project Information) *help	2018: 福岡大学, 薬学部, 助教 2016 – 2017: 国立研究開発法人産業技術総合研究所, 物質計測標準研究部門, 研究員 2015: 国立研究開発法人産業技術総合研究所, 物質計測標準部門, 研究員
Review Section/Research Field	Principal Investigator Physical pharmacy / Analytical chemistry
Keywords	Principal Investigator LC-MS / タンパク質 / ペプチド / アミノ酸 / 誘導体化 / カルボキシル基

Development of sensitive and selective LC-MS method for protein and assessment method for deamidation of protein using carboxy group derivatizationPrincipal Investigator
- Principal Investigator
  
  Sakaguchi Yohei
- Project Period (FY)
  2015 – 2018
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Physical pharmacy
  Analytical chemistry
- Research Institution
  Fukuoka University
  National Institute of Advanced Industrial Science and Technology

All 2018 2017 2016 2015

All Journal Article Presentation

[Journal Article] Quantification of peptides usingN-terminal isotope coding andC-terminal derivatization for sensitive analysis by micro liquid chromatography-tandem mass spectrometry2017
- Author(s)
  Yohei SAKAGUCHI, Tomoya KINUMI, Akiko TAKATSU
- Journal Title
  
  Journal of Mass Spectrometry
  
  Volume: 51 Pages: 1111-1119
- DOI
  10.1002/jms.3845
- Peer Reviewed / Acknowledgement Compliant / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K21662
[Journal Article] Isotope-dilution liquid chromatography-tandem mass spectrometry for sensitive quantification of human insulin in serum using derivatization-technique2017
- Author(s)
  Sakaguchi Yohei、Kinumi Tomoya、Takatsu Akiko
- Journal Title
  
  Analytical Biochemistry
  
  Volume: 537 Pages: 26-32
- DOI
  10.1016/j.ab.2017.08.019
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K21662
[Journal Article] Quantification of glycated N-terminal peptide of hemoglobin using derivatization for multiple functional groups of amino acids followed by liquid chromatography/tandem mass spectrometry.2016
- Author(s)
  Sakaguchi Y, Kinumi T, Yamazaki T, Takatsu A.
- Journal Title
  
  Biomed Chromatogr
  
  Volume: 30 Pages: 280-284
- DOI
  10.1002/bmc.3524
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-15K21662
[Journal Article] Selective and sensitive liquid chromatographic determination method of 5-hydroxyindoles with fluorous and fluorogenic derivatization.2015
- Author(s)
  Yohei Sakaguchi, Jun Ikenaga, Hideyuki Yoshida, Tadashi Hayama, Miki Itoyama, Kenichiro Todoroki, Osamu Imakyure, Masatoshi Yamaguchi, Hitoshi Nohta
- Journal Title
  
  J Pharm Biomed Anal
  
  Volume: 114 Pages: 348-354
- DOI
  10.1016/j.jpba.2015.06.003
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-15K07911, KAKENHI-PROJECT-26410166, KAKENHI-PROJECT-26460182, KAKENHI-PROJECT-15K21662, KAKENHI-PROJECT-25810090, KAKENHI-PROJECT-24619009, KAKENHI-PROJECT-25870994
[Journal Article] 二官能基誘導体化とマイクロＬＣ－ＭＳ／ＭＳによる生理活性ペプチド類の高感度分析2015
- Author(s)
  坂口洋平、絹見朋也、高津章子
- Journal Title
  
  Chromatography
  
  Volume: 36 Pages: 52-52
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] 蛍光誘導体化HPLC法による二枚貝中オカダ酸の分析2018
- Author(s)
  吉田秀幸，河村梨那，中山絵梨奈，古賀鈴依子，矢野（清川）恵奈，坂口洋平，山口政俊，能田均
- Organizer
  日本分析化学会第67年会
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] ヒト血漿中クルクミンのフルオラス誘導体化LC-MS/MS分析2018
- Author(s)
  濱岡祐司，矢野恵奈，巴山忠，古賀鈴依子，坂口洋平，吉田秀幸，能田均
- Organizer
  第31回バイオメディカル分析科学シンポジウム
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] カルボキシル基誘導体化LC-MS法によるタンパク質の脱アミド化評価法の開発2018
- Author(s)
  川末慎葉，坂口洋平，古賀鈴依子，吉田秀幸，能田均
- Organizer
  日本薬学会第139年会
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] キヌレニン鏡像異性体を対象とした高選択的二次元HPLC分析法の開発2018
- Author(s)
  甲斐永美里，古賀鈴依子，坂口洋平，三田真史，井手友美，吉田秀幸，能田均，濵瀬健司
- Organizer
  第36回九州分析化学若手の会夏季セミナー
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] 同位体希釈質量分析法を用いたヒトインスリン認証標準物質の開発2018
- Author(s)
  坂口洋平
- Organizer
  第31回九州分析化学若手の会春の講演会
- Invited
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] フルオラス誘導体化LC-MS法による二枚貝中のオカダ酸分析2018
- Author(s)
  赤穂　健太、坂口　洋平、河村　梨那、吉田　秀幸、古賀　鈴依子、能田　均
- Organizer
  フルオラス科学研究会第11回シンポジウム
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] フルオラス誘導体化LC-MS法による貝毒成分のオカダ酸及びドウモイ酸の分析2018
- Author(s)
  赤穂健太，吉田秀幸，坂口洋平，古賀鈴依子，能田均
- Organizer
  第36回九州分析化学若手の会夏季セミナー
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] パーフルオロポリエーテルカルボン酸の金属塩を利用したリン脂質の選択的抽出及びLC-MS/MS分析2018
- Author(s)
  田坂友里恵，矢野恵奈，巴山忠，古賀鈴依子，坂口洋平，吉田秀幸，能田均
- Organizer
  第31回バイオメディカル分析科学シンポジウム
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] 多次元HPLCを用いる代謝関連キラルアミノ酸の高選択的分析法開発2018
- Author(s)
  古賀鈴依子，甲斐永美里，篠島朋子，三田真史，井手友美，坂口洋平，吉田秀幸，能田均，濵瀬健司
- Organizer
  第14回D-アミノ酸学会学術講演会
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] Development of a carboxyl group derivatization LC method for monitoring the deamidation of protein2017
- Author(s)
  Yohei Sakaguchi, Tomoya Kinumi, Akiko Takatsu
- Organizer
  HPLC2017
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] A dual functional-group derivatization liquid chromatography-tandem mass spectrometry method; application for quantification of human insulin using enzymatic digestion2016
- Author(s)
  Yohei SAKAGUCHI, Tomoya KINUMI, Akiko TAKATSU
- Organizer
  HPLC 2016, 44th International Symposium on High Performance Liquid Phase Separations and Related Techniques
- Place of Presentation
  San Francisco Marriott Marquis、サンフランシスコ、アメリカ
- Year and Date
  2016-06-19
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] カルボキシル基誘導体化LCを用いたタンパク質脱アミド化の評価法の開発2016
- Author(s)
  坂口洋平、絹見朋也、高津章子
- Organizer
  日本分析化学会第65年会
- Place of Presentation
  北海道大学、札幌市
- Year and Date
  2016-09-14
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] 二官能基誘導体化-ＬＣ－ＭＳ／ＭＳによる生理活性ペプチド類の高感度分析2015
- Author(s)
  坂口洋平、絹見朋也、高津章子
- Organizer
  第63回質量分析総合討論会
- Place of Presentation
  つくば市
- Year and Date
  2015-06-17
- Data Source
  KAKENHI-PROJECT-15K21662
[Presentation] 二官能基誘導体化とマイクロＬＣ－ＭＳ／ＭＳによる生理活性ペプチド類の高感度分析2015
- Author(s)
  坂口洋平、絹見朋也、高津章子
- Organizer
  第26回クロマトグラフィー科学会議
- Place of Presentation
  福岡市
- Year and Date
  2015-11-11
- Data Source
  KAKENHI-PROJECT-15K21662

1. Hayama Tadashi

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
2. NOHTA Hitoshi

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
3. ITOYAMA Miki

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
4. 山口政俊

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
5. 吉田秀幸

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
6. 轟木堅一郎

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results

Sakaguchi Yohei 坂口 洋平

Research Projects

Research Products

Co-Researchers

Development of sensitive and selective LC-MS method for protein and assessment method for deamidation of protein using carboxy group derivatizationPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

[Journal Article] Quantification of peptides usingN-terminal isotope coding andC-terminal derivatization for sensitive analysis by micro liquid chromatography-tandem mass spectrometry2017

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Isotope-dilution liquid chromatography-tandem mass spectrometry for sensitive quantification of human insulin in serum using derivatization-technique2017

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Quantification of glycated N-terminal peptide of hemoglobin using derivatization for multiple functional groups of amino acids followed by liquid chromatography/tandem mass spectrometry.2016

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Selective and sensitive liquid chromatographic determination method of 5-hydroxyindoles with fluorous and fluorogenic derivatization.2015

Author(s)

Journal Title

DOI

Data Source

[Journal Article] 二官能基誘導体化とマイクロＬＣ－ＭＳ／ＭＳによる生理活性ペプチド類の高感度分析2015

Author(s)

Journal Title

Data Source

[Presentation] 蛍光誘導体化HPLC法による二枚貝中オカダ酸の分析2018

Author(s)

Organizer

Data Source

[Presentation] ヒト血漿中クルクミンのフルオラス誘導体化LC-MS/MS分析2018

Author(s)

Organizer

Data Source

[Presentation] カルボキシル基誘導体化LC-MS法によるタンパク質の脱アミド化評価法の開発2018

Author(s)

Organizer

Data Source

[Presentation] キヌレニン鏡像異性体を対象とした高選択的二次元HPLC分析法の開発2018

Author(s)

Organizer

Data Source

[Presentation] 同位体希釈質量分析法を用いたヒトインスリン認証標準物質の開発2018

Author(s)

Organizer

Data Source

[Presentation] フルオラス誘導体化LC-MS法による二枚貝中のオカダ酸分析2018

Author(s)

Organizer

Data Source

[Presentation] フルオラス誘導体化LC-MS法による貝毒成分のオカダ酸及びドウモイ酸の分析2018

Author(s)

Organizer

Data Source

[Presentation] パーフルオロポリエーテルカルボン酸の金属塩を利用したリン脂質の選択的抽出及びLC-MS/MS分析2018

Author(s)

Organizer

Data Source

[Presentation] 多次元HPLCを用いる代謝関連キラルアミノ酸の高選択的分析法開発2018

Author(s)

Organizer

Data Source

[Presentation] Development of a carboxyl group derivatization LC method for monitoring the deamidation of protein2017

Author(s)

Organizer

Data Source

[Presentation] A dual functional-group derivatization liquid chromatography-tandem mass spectrometry method; application for quantification of human insulin using enzymatic digestion2016

Author(s)

Organizer

Place of Presentation

Year and Date

Data Source

Sakaguchi Yohei 坂口洋平