Yuki Ohmuro 大室有紀

… Alternative Names

松山有紀

MATSUYAMA Yuki 松山有紀

OHMURO Yuki

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Researcher Number	30571088
Other IDs
Affiliation (based on the past Project Information) *help	2021: 東京工業大学, 資源化学研究所, JSPS特別研究員 2019: 東京工業大学, 化学生命科学研究所, 助教 2019: 東京工業大学, 資源化学研究所, JSPS特別研究員 2016 – 2019: 東京工業大学, 科学技術創成研究院, 助教 2017: 東京工業大学, 科学技術創成研究院化学生命科学研究所, 助教 … More 2014: 学術振興会RPD/神戸大学, 大学院工学研究科, 特命助教 2014: 神戸大学, 大学院工学研究科, 特命助教 2012: 東京大学, 工学(系)研究科(研究院), 研究員 2012: 東京大学, 特任研究員 2010 – 2011: 東京大学, 大学院・工学系研究科, 特任研究員 Less
Review Section/Research Field	Principal Investigator Biofunction/Bioprocess / Biofunction/Bioprocess Except Principal Investigator Biofunction/Bioprocess / Biofunction/Bioprocess / Medium-sized Section 27:Chemical engineering and related fields / Biological Sciences
Keywords	Principal Investigator タンパク質間相互作用検出系 / 安定性 / 明るさ / 立体障害 / フォールディング / 数理モデル / 膜タンパク質 / タンパク質間相互作用検出 / タンパク質相互作用検出系 / 発光酵素 / タンパク質間相互作用 … More Except Principal Investigator … More 抗体 / バイオセンサー / 免疫測定 / 蛋白質 / 抗体工学 / バイオテクノロジー / 蛍光 / タンパク質 / プロテオーム / 免疫学 / ケミカルバイオロジー / クエンチ / 抗原 / バイオイメージング / immunoassay / antibody / 可視化 / 生物・生体工学 / ペプチド / タンパク質工学 / 蛍光クエンチ / 免疫測定法 / 抗原抗体反応 / ペプチド工学 / マイクロアレイ / 結合タンパク質 / 部位特異的修飾 / 蛍光プローブ / 解離速度 / 結合速度 / リンカー工学 / アビディティー / 抗体結合タンパク質 / リンカーペプチド / 酵素 / 結合タンパク / 融合タンパク質 / avidity / affinity / purification / 臨床診断 / バイオチップ / 蛍光消光 / 蛍光エネルギー移動 / 細胞分化 / イメージング / bioimaging / chemical biology / protein engineering / biochip / cell-free translation / fluorescence quenching / タンパク質間相互作用 / 表面プラズモン共鳴法 / 分析科学 / 細胞信号伝達 / 翻訳後修飾 / 分析化学 Less

New development in fluorescent immunosensor based on the quench-release principle by protein and peptide engineering
- Principal Investigator
  
  Ueda Hiroshi
- Project Period (FY)
  2018 – 2021
- Research Category
  
  Grant-in-Aid for Scientific Research (A)
- Review Section
  
  Medium-sized Section 27:Chemical engineering and related fields
- Research Institution
  Tokyo Institute of Technology
Development of sensitive multi-colour detection using the protein-protein interaction assay, FlimPIAPrincipal Investigator
- Principal Investigator
  
  Yuki Ohmuro
- Project Period (FY)
  2017 – 2019
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Biofunction/Bioprocess
- Research Institution
  Tokyo Institute of Technology
Development of innovative immunoassay through the application of quench-release principle to natural antibodies
- Principal Investigator
  
  UEDA Hiroshi
- Project Period (FY)
  2015 – 2017
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  Biofunction/Bioprocess
- Research Institution
  Tokyo Institute of Technology
Creation of Ultra Super Fusion Protein for Antibody Binding and Purification
- Principal Investigator
  
  UEDA Hiroshi
- Project Period (FY)
  2013 – 2014
- Research Category
  
  Grant-in-Aid for Challenging Exploratory Research
- Research Field
  
  Biofunction/Bioprocess
- Research Institution
  Tokyo Institute of Technology
Novel Expansion of Bioanalysis Based on the Fluorescence Quench-Release Principle
- Principal Investigator
  
  UEDA Hiroshi
- Project Period (FY)
  2012 – 2014
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  Biofunction/Bioprocess
- Research Institution
  Tokyo Institute of Technology
迅速・高感度な多色細胞内マルチ蛋白質間相互作用検出系の構築Principal Investigator
- Principal Investigator
  
  松山有紀 (大室有紀)
- Project Period (FY)
  2012
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Biofunction/Bioprocess
- Research Institution
  The University of Tokyo
Construction of versatile fluoroimmunoassay probe based on quench-release principle
- Principal Investigator
  
  UEDA Hiroshi
- Project Period (FY)
  2011 – 2012
- Research Category
  
  Grant-in-Aid for Challenging Exploratory Research
- Research Field
  
  Biofunction/Bioprocess
- Research Institution
  Tokyo Institute of Technology
  The University of Tokyo
抗リン酸化ペプチド抗体を用いた細胞周期制御機構の可視化
- Principal Investigator
  
  UEDA Hiroshi
- Project Period (FY)
  2010 – 2011
- Research Category
  
  Grant-in-Aid for Scientific Research on Priority Areas
- Review Section
  
  Biological Sciences
- Research Institution
  The University of Tokyo

[Book] Protein-Protein Interaction Assays Using Split-NanoLuc; in Bioluminescence - Analytical Applications and Basic Biology2019
- Author(s)
  Ohmuro-Matsuyama Yuki、Ueda Hiroshi
- Total Pages
  16
- Publisher
  Intech Open
- ISBN
  9781789847840
- Data Source
  KAKENHI-PROJECT-18H03851
[Book] 発光イメージング実験ガイド2019
- Author(s)
  大室　有紀, 上田　宏
- Total Pages
  6
- Publisher
  羊土社
- ISBN
  9784758122405
- Data Source
  KAKENHI-PROJECT-17K06920
[Book] 分割NanoLucを用いた相互作用検出系による低分子の免疫測定; in 実験医学別冊　発光イメージング実験ガイド (Eds. 永井健治・小澤岳昌）, 194-1972019
- Author(s)
  大室有紀，上田宏
- Total Pages
  4
- Publisher
  羊土社
- ISBN
  9784758122405
- Data Source
  KAKENHI-PROJECT-18H03851
[Book] Bioluminescence2019
- Author(s)
  Ohmuro-Matsuyama Y, Ueda H
- Total Pages
  16
- Publisher
  IntechOpen
- Data Source
  KAKENHI-PROJECT-17K06920
[Book] Protein-Protein Interaction Assays2018
- Author(s)
  Yuki Omuro, Hiroshi Ueda
- Total Pages
  17
- Publisher
  IntechOpen
- ISBN
  9781789233919
- Data Source
  KAKENHI-PROJECT-17K06920
[Book] Method in Molecular Biology2017
- Author(s)
  大室有紀・上田宏
- Total Pages
  12
- Publisher
  SpringerLink
- ISBN
  9781493969388
- Data Source
  KAKENHI-PROJECT-17K06920
[Book] "Detection of protein phosphorylation by open-sandwich immunoassay" in Integrative Proteomics (H.-C.Leung) ISBN : 978-953-51-0070-62012
- Author(s)
  Y.Ohmuro-Matsuyama, M.Inagaki, H.Ueda
- Total Pages
  18
- Publisher
  InTech, Rijeka, Croatia
- Data Source
  KAKENHI-PUBLICLY-22019007
[Book] 抗リン酸化抗体を用いた生細胞での蛋白質リン酸化検出(佐方功幸,稲垣昌樹,岸本健雄編「細胞周期フロンティア」p.11-17)2010
- Author(s)
  大室(松山)有紀, 北岡優一, 上田宏
- Total Pages
  7
- Publisher
  共立出版
- Data Source
  KAKENHI-PUBLICLY-22019007
[Journal Article] Miniaturization of Bright Light-Emitting Luciferase ALuc: picALuc2022
- Author(s)
  Ohmuro-Matsuyama Yuki、Furuta Tadaomi、Matsui Hayato、Kanai Masaki、Ueda Hiroshi
- Journal Title
  
  ACS Chemical Biology
  
  Volume: 17 Issue: 4 Pages: 864-872
- DOI
  10.1021/acschembio.1c00897
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-18H03851
[Journal Article] BRET Q-Body: A Ratiometric Quench-based Bioluminescent Immunosensor Made of Luciferase-Dye-Antibody Fusion with Enhanced Response2021
- Author(s)
  Takahashi Riho、Yasuda Takanobu、Ohmuro-Matsuyama Yuki、Ueda Hiroshi
- Journal Title
  
  Analytical Chemistry
  
  Volume: 93 Issue: 21 Pages: 7571-7578
- DOI
  10.1021/acs.analchem.0c05217
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-18H03851
[Journal Article] Improving the Stability of Protein-Protein Interaction Assay FlimPIA Using a Thermostabilized Firefly Luciferase2021
- Author(s)
  Ohmuro-Matsuyama Yuki、Gomi Keiko、Shimoda Takuya、Yamaji Hideki、Ueda Hiroshi
- Journal Title
  
  Frontiers in Bioengineering and Biotechnology
  
  Volume: 9 Pages: 778120-778120
- DOI
  10.3389/fbioe.2021.778120
- NAID
  120007172604
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18H03851
[Journal Article] Simple Fluorogenic Cellular Assay for Histone Deacetylase Inhibitors Based on Split-Yellow Fluorescent Protein and Intrabodies2021
- Author(s)
  Ohmuro-Matsuyama Yuki、Kitaguchi Tetsuya、Kimura Hiroshi、Ueda Hiroshi
- Journal Title
  
  ACS Omega
  
  Volume: 6 Issue: 15 Pages: 10039-10046
- DOI
  10.1021/acsomega.0c06281
- NAID
  120007034011
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PLANNED-18H05527, KAKENHI-PROJECT-18H03851
[Journal Article] Preparation of an antigen-responsive fluorogenic immunosensor by tyrosine chemical modification of the antibody complementarity determining region2021
- Author(s)
  Sato Shinichi、Matsumura Masaki、Ueda Hiroshi、Nakamura Hiroyuki
- Journal Title
  
  Chemical Communications
  
  Volume: 57 Issue: 76 Pages: 9760-9763
- DOI
  10.1039/d1cc03231c
- Peer Reviewed
- Data Source
  KAKENHI-PUBLICLY-20H04699, KAKENHI-PROJECT-18H03851, KAKENHI-PROJECT-19H02848
[Journal Article] An Open Sandwich Immunochromatography for Non-competitive Detection of Small Antigens2021
- Author(s)
  HARADA Yoshitaka、OHMURO-MATSUYAMA Yuki、TSUNA Mika、UEDA Hiroshi
- Journal Title
  
  Anal. Sci.
  
  Volume: 37 Issue: 3 Pages: 455-459
- DOI
  10.2116/analsci.20SCP06
- NAID
  130007996238
- ISSN
  0910-6340, 1348-2246
- Year and Date
  2021-03-10
- Language
  English
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-18H03851
[Journal Article] Site-Selective Protein Chemical Modification of Exposed Tyrosine Residues Using Tyrosine Click Reaction2020
- Author(s)
  Sato Shinichi、Matsumura Masaki、Kadonosono Tetsuya、Abe Satoshi、Ueno Takafumi、Ueda Hiroshi、Nakamura Hiroyuki
- Journal Title
  
  Bioconjugate Chemistry
  
  Volume: in press Issue: 5 Pages: 1417-1424
- DOI
  10.1021/acs.bioconjchem.0c00120
- Peer Reviewed
- Data Source
  KAKENHI-PLANNED-18H05421, KAKENHI-PROJECT-18K19143, KAKENHI-PROJECT-19H02830, KAKENHI-PROJECT-18H03851, KAKENHI-PROJECT-19H02848, KAKENHI-PUBLICLY-20H04699
[Journal Article] Creation of a Nanobody-Based Fluorescent Immunosensor Mini Q-body for Rapid Signal-On Detection of Small Hapten Methotrexate2020
- Author(s)
  Inoue Akihito、Ohmuro-Matsuyama Yuki、Kitaguchi Tetsuya、Ueda Hiroshi
- Journal Title
  
  ACS Sensors
  
  Volume: 5 Issue: 11 Pages: 3457-3464
- DOI
  10.1021/acssensors.0c01404
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-18H03851
[Journal Article] Detection and Destruction of HER2-positive Cancer Cells by Ultra Quenchbody-siRNA Complex2020
- Author(s)
  Dong J, Oka Y, Jeong HJ, Ohmuro-Matsuyama Y, Ueda H
- Journal Title
  
  Biotechnol. Bioeng.
  
  Volume: 117 Issue: 5 Pages: 1259-1269
- DOI
  10.1002/bit.27302
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K06920, KAKENHI-PROJECT-18H03851
[Journal Article] PM Q-probe: A fluorescent binding protein that converts many antibodies to a fluorescent biosensor2020
- Author(s)
  Dong Jinhua、Miyake Chihiro、Yasuda Takanobu、Oyama Hiroyuki、Morita Izumi、Tsukahara Tomoya、Takahashi Masaki、Jeong Hee-Jin、Kitaguchi Tetsuya、Kobayashi Norihiro、Ueda Hiroshi
- Journal Title
  
  Biosensors and Bioelectronics
  
  Volume: 165 Pages: 112425-112425
- DOI
  10.1016/j.bios.2020.112425
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K07021, KAKENHI-PROJECT-19K07033, KAKENHI-PROJECT-19K10697, KAKENHI-PROJECT-18H03851
[Journal Article] Green fluorescent protein-based lactate and pyruvate indicators suitable for biochemical assays and live cell imaging.2020
- Author(s)
  Harada K*, Chihara T*, Hayasaka Y*, Mita M, Takizawa M, Ishida K, Arai M, Tsuno S, Matsumoto M, Ishihara T, Ueda H, Kitaguchi T, Tsuboi T. *Equal contribution
- Journal Title
  
  Scientific Reports
  
  Volume: 10 Issue: 1 Pages: 1-11
- DOI
  10.1038/s41598-020-76440-4
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-19K23820, KAKENHI-PUBLICLY-20H04765, KAKENHI-PUBLICLY-20H04836, KAKENHI-PROJECT-18H03851, KAKENHI-PROJECT-20H04121, KAKENHI-PROJECT-20J14411, KAKENHI-PROJECT-17K08529, KAKENHI-PROJECT-19H03326, KAKENHI-PROJECT-17H06113
[Journal Article] Graphene Field Effect Transistor-Based Immunosensor for Ultrasensitive Noncompetitive Detection of Small Antigens2020
- Author(s)
  Kanai Y, Ohmuro-Matsuyama Y, Tanioku M, Ushiba S, Ono T, Inoue K, Kitaguchi T, Kimura M, Ueda H, Matsumoto K
- Journal Title
  
  ACS Sens.
  
  Volume: 5 Issue: 1 Pages: 24-28
- DOI
  10.1021/acssensors.9b02137
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K06920, KAKENHI-PROJECT-18H03851, KAKENHI-PROJECT-18K14107
[Journal Article] Development of a fluvoxamine detection system using a Quenchbody, a novel fluorescent biosensor.2019
- Author(s)
  Sasao, A., Takaki, M., Jeong, H. J., Yonemitsu, K., Ohtsu, Y., Tsutsumi, H., Furukawa, S., Morioka, H., Ueda, H. and Nishitani, Y.
- Journal Title
  
  Drug Test Anal.
  
  Volume: 11 Issue: 4 Pages: 601-609
- DOI
  10.1002/dta.2520
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17H04105, KAKENHI-PROJECT-16K09211, KAKENHI-PROJECT-18H03851
[Journal Article] Intrabody-based FRET probe to visualize endogenous histone acetylation2019
- Author(s)
  Chung Chan-I、Sato Yuko、Ohmuro-Matsuyama Yuki、Machida Shinichi、Kurumizaka Hitoshi、Kimura Hiroshi、Ueda Hiroshi
- Journal Title
  
  Scientific Reports
  
  Volume: 9 Issue: 1 Pages: 10188-10188
- DOI
  10.1038/s41598-019-46573-2
- NAID
  120006936925
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PLANNED-18H05527, KAKENHI-PLANNED-18H05534, KAKENHI-PROJECT-17K06920, KAKENHI-PROJECT-17H01417, KAKENHI-PROJECT-18H03851
[Journal Article] Transmembrane Signaling on a Protocell: Creation of Receptor-Enzyme Chimeras for Immunodetection of Specific Antibodies and Antigens2019
- Author(s)
  Su J, Kitaguchi T, Ohmuro-Matsuyama Y, Seah T, Ghadessy FJ, Hoon S, Ueda H
- Journal Title
  
  Sci Rep.
  
  Volume: 9 Issue: 1 Pages: 18189-18189
- DOI
  10.1038/s41598-019-54539-7
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K06920, KAKENHI-PROJECT-18H03851
[Journal Article] Non-Steady State Analysis of Enzyme Kinetics in Real Time Elucidates Substrate Association and Dissociation Rates: Demonstration With Analysis of Firefly Luciferase Mutants2019
- Author(s)
  Dale R, Ohmuro-Matsuyama Y, Ueda H, Kato N
- Journal Title
  
  Biochmistry
  
  Volume: 58 Issue: 23 Pages: 2695-2702
- DOI
  10.1021/acs.biochem.9b00272
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K06920, KAKENHI-PROJECT-18H03851
[Journal Article] Creation of Stable and Strictly Regulated Enzyme Switch for Signal-On Immunodetection of Various Small Antigens2019
- Author(s)
  Su J, Beh C, Ohmuro-Matsuyama Y, Kitaguchi T, Hoon S, Ueda H
- Journal Title
  
  J Biosci Bioeng.
  
  Volume: 128 Issue: 6 Pages: 677-682
- DOI
  10.1016/j.jbiosc.2019.05.015
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K06920, KAKENHI-PROJECT-18H03851
[Journal Article] Homogeneous Noncompetitive Luminescent Immunodetection of Small Molecules by Ternary Protein Fragment Complementation2018
- Author(s)
  大室有紀・上田宏
- Journal Title
  
  Analytical Chemistry
  
  Volume: 90 Issue: 5 Pages: 3001-3004
- DOI
  10.1021/acs.analchem.7b05140
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K06920, KAKENHI-PROJECT-15H04191
[Journal Article] Noncompetitive homogeneous immunodetection of small molecules based on beta-glucuronidase complementation2018
- Author(s)
  Su Jiulong、Dong Jinhua、Kitaguchi Tetsuya、Ohmuro-Matsuyama Yuki、Ueda Hiroshi
- Journal Title
  
  The Analyst
  
  Volume: 143 Issue: 9 Pages: 2096-2101
- DOI
  10.1039/c8an00074c
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18H03851
[Journal Article] Evaluation of protein-ligand interactions using the luminescent interaction assay FlimPIA with streptavidin-biotin linkage.2018
- Author(s)
  Ohmuro-Matsuyama Y, Yamashita T, Gomi K, Yamaji H, Ueda H.
- Journal Title
  
  Anal Biochem.
  
  Volume: 563 Pages: 61-66
- DOI
  10.1016/j.ab.2018.10.010
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K06920, KAKENHI-PROJECT-18H03851
[Journal Article] A Novel Protein-Protein Interaction Assay Based on the Functional Complementation of Mutant Firefly Luciferases: Split Structure Versus Divided Reaction2018
- Author(s)
  Ohmuro-Matsuyama Yuki、Ueda Hiroshi
- Journal Title
  
  Protein-Protein Interaction Assays
  
  Volume: 1 Pages: 11-27
- DOI
  10.5772/intechopen.75644
- NAID
  120006582691
- ISBN
  9781789233902, 9781789233919
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18H03851
[Journal Article] Ultrasensitive Firefly Luminescent Intermediate-Based Protein-Protein Interaction Assay (FlimPIA) Based on the Functional Complementation of Mutant Firefly Luciferases2017
- Author(s)
  Ohmuro-Matsuyama Yuki、Ueda Hiroshi
- Journal Title
  
  Methods in Molecular Biology
  
  Volume: 1596 Pages: 119-130
- DOI
  10.1007/978-1-4939-6940-1_8
- ISBN
  9781493969388, 9781493969401
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-15H04191
[Journal Article] Development of a Quenchbody for the Detection and Imaging of the Cancer-Related Tight-Junction-Associated Membrane Protein Claudin2017
- Author(s)
  Jeong Hee-Jin、Kawamura Takuya、Iida Manami、Kawahigashi Yumi、Takigawa Mutsumi、Ohmuro-Matsuyama Yuki、Chung Chan-I、Dong Jinhua、Kondoh Masuo、Ueda Hiroshi
- Journal Title
  
  Analytical Chemistry
  
  Volume: 89 Issue: 20 Pages: 10783-10789
- DOI
  10.1021/acs.analchem.7b02047
- NAID
  120006825608
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15H04191
[Journal Article] Development of a fluorescent protein-antibody Forster resonance energy transfer probe for the detection and imaging of osteocalcin2017
- Author(s)
  Chung Chan-I、Makino Ryoji、Ohmuro-Matsuyama Yuki、Ueda Hiroshi
- Journal Title
  
  Journal of Bioscience and Bioengineering
  
  Volume: 123 Issue: 2 Pages: 272-276
- DOI
  10.1016/j.jbiosc.2016.09.003
- NAID
  40021112823
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-15H04191
[Journal Article] Improved sensitivity of firefly luminescent intermediate-based protein interaction assay using Ser 440 mutant with lower adenylation activity.2017
- Author(s)
  大室有紀・山下貴宏・LIn Huan・山地秀樹・上田宏
- Journal Title
  
  Luminescence
  
  Volume: 33 Issue: 1 Pages: 125-130
- DOI
  10.1002/bio.3381
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K06920, KAKENHI-PROJECT-15H04191
[Journal Article] One-pot construction of Quenchbodies using antibody-binding proteins2016
- Author(s)
  Jeong Hee-Jin、Kojima Tomoki、Dong Jinhua、Ohashi Hiroyuki、Ueda Hiroshi
- Journal Title
  
  Analytical Methods
  
  Volume: 8 Issue: 43 Pages: 7774-7779
- DOI
  10.1039/c6ay02108e
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15H04191
[Journal Article] Mathematical model of the firefly luciferase complementation assay reveals a non-linear relationship between the detected luminescence and the affinity of the protein pair being analyzed2016
- Author(s)
  R. Dale, Y. Ohmuro-Matsuyama, H. Ueda and N. Kato
- Journal Title
  
  PLoS ONE
  
  Volume: 11 Issue: 2 Pages: e0148256-e0148256
- DOI
  10.1371/journal.pone.0148256
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15H04191
[Journal Article] Ultra sensitive firefly luciferase-based protein-protein interaction assay (FlimPIA) attained by hinge region engineering and optimized reaction conditions2016
- Author(s)
  M. Kurihara, Y. Ohmuro-Matsuyama, K. Ayabe, T. Yamashita, H. Yamaji and H. Ueda
- Journal Title
  
  Biotechnology Journal
  
  Volume: 11 Issue: 1 Pages: 91-99
- DOI
  10.1002/biot.201500189
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-15H04191
[Journal Article] A Protein-Protein Interaction Assay FlimPIA Based on the Functional Complementation of Mutant Firefly Luciferases2016
- Author(s)
  Ohmuro-Matsuyama Yuki、Ueda Hiroshi
- Journal Title
  
  Methods in Molecular Biology
  
  Volume: 1461 Pages: 131-142
- DOI
  10.1007/978-1-4939-3813-1_10
- ISBN
  9781493938117, 9781493938131
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-15H04191
[Journal Article] Insight into the Working Mechanism of Quenchbody: Transition of the Dye around Antibody Variable Region That Fluoresces upon Antigen Binding2016
- Author(s)
  Hiroyuki Ohashi, Toshio Matsumoto, Hee-JinJeong, Jinhua Dong,Ryoji Abe, Hiroshi Ueda
- Journal Title
  
  Bioconjugate Chemistry
  
  Volume: 27(10) Issue: 10 Pages: 2248-2253
- DOI
  10.1021/acs.bioconjchem.6b00217
- NAID
  120006795377
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-26420793, KAKENHI-PROJECT-15H04191
[Journal Article] Electrostatic engineering of the interface between heavy and light chains promotes antibody Fab fragment production2016
- Author(s)
  Ohmuro-Matsuyama Yuki、Mori Keita、Hamada Hirotsugu、Ueda Hiroshi、Yamaji Hideki
- Journal Title
  
  Cytotechnology
  
  Volume: 69 Issue: 3 Pages: 469-475
- DOI
  10.1007/s10616-016-9955-4
- NAID
  120006323719
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-15H04191, KAKENHI-PROJECT-15H04195
[Journal Article] Improved protein-protein interaction assay FlimPIA by the entrapment of luciferase conformation2014
- Author(s)
  Yuki Ohmuro-Matsuyama, Yuko Hara, and Hiroshi Ueda
- Journal Title
  
  Analytical Chemistry
  
  Volume: (印刷中) Issue: 4 Pages: 2013-2018
- DOI
  10.1021/ac403065v
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-12J40072, KAKENHI-PROJECT-24360336, KAKENHI-PROJECT-25630370
[Journal Article] Detection of vimentin serine phosphorylation by multicolor Quenchbodies2013
- Author(s)
  H.-J. Jeong, Y. Ohmuro-Matsuyama, H. Ohashi, F. Ohsawa, Y. Tatsu, M. Inagaki, and H. Ueda
- Journal Title
  
  Biosens. Bioelectron
  
  Volume: 40 Pages: 17-23
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23656517
[Journal Article] Detection of vimentin serine phosphorylation by multicolor Quenchbodies2013
- Author(s)
  Jeong, H.J.
- Journal Title
  
  Biosensors and Bioelectronics
  
  Volume: 40 Issue: 1 Pages: 17-23
- DOI
  10.1016/j.bios.2012.06.030
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23656517, KAKENHI-PROJECT-24360336
[Journal Article] Demonstration of protein-fragment complementation assay using purified firefly luciferase fragments2013
- Author(s)
  Y. Ohmuro-Matsuyama, C.I. Chung, and H. Ueda
- Journal Title
  
  BMC Biotechnol
  
  Volume: 13 Pages: 31-31
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23656517
[Journal Article] A protein-protein interaction assay based on the functional complementa tion of mutant firefly luciferase.2013
- Author(s)
  Yuki Ohmuro-Matsuyama, Kota Nakano, Aoi Kimura, Keiichi Ayabe and Hiroshi Ueda
- Journal Title
  
  Analytieal Chemistry
  
  Volume: 85 Issue: 16 Pages: 7935-7940
- DOI
  10.1021/ac4016825
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-12J40072, KAKENHI-PLANNED-21115008, KAKENHI-PROJECT-24360336
[Journal Article] Demonstration of protein-fragment complementation assay using purified firefly luciferase fragments2013
- Author(s)
  Ohmuro-Matsuyama, Y.
- Journal Title
  
  BMC Biotechnology
  
  Volume: 13 Issue: 1 Pages: 31-31
- DOI
  10.1186/1472-6750-13-31
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23656517, KAKENHI-PROJECT-24360336
[Patent] 複数の蛍光色素を結合させたペプチドを用いるホモジニアス免疫測定法2020
- Inventor(s)
  上田宏，北口哲也，大室有紀，安田貴信，井上暁人
- Industrial Property Rights Holder
  上田宏，北口哲也，大室有紀，安田貴信，井上暁人
- Industrial Property Rights Type
  特許
- Filing Date
  2020
- Overseas
- Data Source
  KAKENHI-PROJECT-18H03851
[Patent] レポータータンパク質断片を付加した組換え抗体を用いるホモジニアス免疫測定法2020
- Inventor(s)
  上田宏，上野慶行，北口哲也，大室有紀
- Industrial Property Rights Holder
  上田宏，上野慶行，北口哲也，大室有紀
- Industrial Property Rights Type
  特許
- Filing Date
  2020
- Overseas
- Data Source
  KAKENHI-PROJECT-18H03851
[Patent] ホモジニアス免疫測定法に適した酵素変異体2020
- Inventor(s)
  上田宏，蘇九龍，北口哲也，大室有紀
- Industrial Property Rights Holder
  上田宏，蘇九龍，北口哲也，大室有紀
- Industrial Property Rights Type
  特許
- Filing Date
  2020
- Overseas
- Data Source
  KAKENHI-PROJECT-18H03851
[Patent] バイオセンサー2019
- Inventor(s)
  上田宏,蘇九龍,北口哲也,大室有紀, S.Hoon, C.Beh
- Industrial Property Rights Holder
  上田宏,蘇九龍,北口哲也,大室有紀, S.Hoon, C.Beh
- Industrial Property Rights Type
  特許
- Filing Date
  2019
- Overseas
- Data Source
  KAKENHI-PROJECT-18H03851
[Patent] ホモジニアス免疫測定法に適した酵素変異体2019
- Inventor(s)
  上田宏，蘇九龍，北口哲也，大室有紀
- Industrial Property Rights Holder
  上田宏，蘇九龍，北口哲也，大室有紀
- Industrial Property Rights Type
  特許
- Filing Date
  2019
- Data Source
  KAKENHI-PROJECT-18H03851
[Patent] 複数の蛍光色素を結合させたペプチドを用いるホモジニアス免疫測定法2019
- Inventor(s)
  上田宏，北口哲也，大室有紀，安田貴信，井上暁人
- Industrial Property Rights Holder
  上田宏，北口哲也，大室有紀，安田貴信，井上暁人
- Industrial Property Rights Type
  特許
- Filing Date
  2019
- Data Source
  KAKENHI-PROJECT-18H03851
[Patent] 抗原検出又は測定用キット2019
- Inventor(s)
  上田宏，大室有紀，三宅千絢，塚原知也
- Industrial Property Rights Holder
  上田宏，大室有紀，三宅千絢，塚原知也
- Industrial Property Rights Type
  特許
- Filing Date
  2019
- Overseas
- Data Source
  KAKENHI-PROJECT-18H03851
[Patent] バイオセンサー2019
- Inventor(s)
  Su, Kitaguchi, Ohmuro et al.
- Industrial Property Rights Holder
  Su, Kitaguchi, Ohmuro et al.
- Industrial Property Rights Type
  特許
- Filing Date
  2019
- Overseas
- Data Source
  KAKENHI-PROJECT-17K06920
[Patent] レポータータンパク質断片を付加した組換え抗体を用いるホモジニアス免疫測定法2019
- Inventor(s)
  上田宏，上野慶行，北口哲也，大室有紀
- Industrial Property Rights Holder
  上田宏，上野慶行，北口哲也，大室有紀
- Industrial Property Rights Type
  特許
- Filing Date
  2019
- Data Source
  KAKENHI-PROJECT-18H03851
[Patent] バイオセンサー2018
- Inventor(s)
  上田宏, 蘇九龍, 北口哲也, 大室有紀, 他
- Industrial Property Rights Holder
  東京工業大学，A*STAR
- Industrial Property Rights Type
  特許
- Filing Date
  2018
- Data Source
  KAKENHI-PROJECT-18H03851
[Patent] 抗原検出又は測定用キット2018
- Inventor(s)
  上田宏, 大室有紀, 三宅千絢他1名
- Industrial Property Rights Holder
  東京工業大学
- Industrial Property Rights Type
  特許
- Filing Date
  2018
- Overseas
- Data Source
  KAKENHI-PROJECT-15H04191
[Patent] 抗原検出又は測定用キット2017
- Inventor(s)
  上田宏，大室有紀，三宅千絢
- Industrial Property Rights Holder
  東京工業大学
- Industrial Property Rights Type
  特許
- Filing Date
  2017
- Data Source
  KAKENHI-PROJECT-15H04191
[Presentation] Development of patrol yeasts for the detection of toxic substances in foods2020
- Author(s)
  Jiulong Su, Yasuyuki Nakamura, Tetsuya Kitaguchi, Jun Ishii, Akihito Kondo, Norihiro Kobayashi, Shiro Miyake, Hiroshi Ueda
- Organizer
  第93回日本生化学会大会（オンライン）
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] YFP断片融合抗体プローブを用いたヒストン翻訳後修飾検出系の構築2019
- Author(s)
  大室（松山）有紀, 北口哲也, 木村宏, 上田宏
- Organizer
  第71会生物工学会退会
- Data Source
  KAKENHI-PROJECT-17K06920
[Presentation] コイルドコイル形成ペプチドを利用した蛍光ラベル化法による抗体断片の迅速FRETバイオセンサー化2019
- Author(s)
  安田貴信、井上暁人、大室有紀、北口哲也、上田宏
- Organizer
  第19回日本蛋白質科学会年会第71回日本細胞生物学会大会合同年次大会, 神戸国際会議場
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] 抗原結合依存的に発光活性を増大させる免疫センサータンパク複合体の開発2019
- Author(s)
  上野慶行・安田貴信・大室有紀・北口哲也・上田宏
- Organizer
  第92回日本生化学会大会
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] 抗原依存的に発光活性が増大する免疫センサータンパク質Hi-Bodyの開発2019
- Author(s)
  上野慶行・安田貴信・大室有紀・北口哲也・上田宏
- Organizer
  第19回日本蛋白質科学会年会第71回日本細胞生物学会大会合同年次大会, 神戸国際会議場
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] 発光酵素を利用した蛋白質間相互作用検出系・免疫測定法2019
- Author(s)
  大室有紀・上田宏
- Organizer
  第４回化学発光イメージングワークショップ
- Invited
- Data Source
  KAKENHI-PROJECT-17K06920
[Presentation] グラフェン電界効果トランジスタを利用したオープンサンドイッチ免疫測定法2019
- Author(s)
  大室（松山）有紀、金井　康
- Organizer
  アライアンス若手交流会
- Invited
- Data Source
  KAKENHI-PROJECT-17K06920
[Presentation] Improved stability of 3-split Nanoluc system by introducing hydrophilic mutations in LcBiT probe2019
- Author(s)
  Junetae PARK, Yuki OHMURO-MATSUYAMA, Masayoshi TANAKA, Mina OKOCHI, Hiroshi UEDA
- Organizer
  酵素工学研究会第82回講演会
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] 小さく、高輝度・高発現なALucの作製2019
- Author(s)
  大室（松山）有紀、金　誠培、上田　宏
- Organizer
  生物発光化学発光研究会第35会学術講演会
- Data Source
  KAKENHI-PROJECT-17K06920
[Presentation] Creation of a novel sensor enzyme and its integration into protocell for digital detection of various anti-epitope tag antibodies2019
- Author(s)
  Jiulong SU, Cyrus BEH, Tetsuya KITAGUCHI, Yuki OHMURO, Theresa SEAH, Shawn HOON, Hiroshi UEDA
- Organizer
  18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019)
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] グラフェンFETにおけるヒト血清中でのオープンサンドイッチ免疫測定法による低分子ペプチドの検出2019
- Author(s)
  金井康, 大室有紀, 谷奥正巳, 牛場翔太, 小野尭生, 井上恒一, 木村雅彦, 上田宏, 松本和彦
- Organizer
  第66回応用物理学会学術講演会
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] Creation of Novel Enzyme Immunosensors and Its Integration to Protocell for Digital Detection of Various anti-Peptide Antibodies2019
- Author(s)
  Jiulong SU, Cyrus BEH, Tetsuya KITAGUCHI, Yuki OHMURO, Theresa SEAH, Shawn HOON, Hiroshi UEDA
- Organizer
  The 14th Asian Congress on Biotechnology
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] YFP断片融合抗体プローブを用いたヒストン翻訳後修飾検出系の構築2019
- Author(s)
  大室　有紀, 北口哲也, 木村　宏, 上田　宏
- Organizer
  2019年度日本生物工学会大会
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] 分割YFP融合Intrabodyを用いたせい細胞内ヒストンアセチル化検出プローブの構築2019
- Author(s)
  大室（松山）有紀, 北口哲也, 木村宏, 上田宏
- Organizer
  第71回日本細胞生物学会大会合同年次大会
- Data Source
  KAKENHI-PROJECT-17K06920
[Presentation] コイルドコイル形成ペプチドを利用した蛍光ラベル化法による抗体断片のバイオセンサー化2019
- Author(s)
  安田貴信、井上暁人、大室有紀、北口哲也、上田宏
- Organizer
  2019年度日本生化学会関東支部例会
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] Beyond Q-body: 色素修飾が不要な新規免疫測定素子の構築2019
- Author(s)
  上田宏, 安田貴信, 上野慶行, 井上暁人,髙橋昌樹, 大室有紀, 北口哲也, 董金華
- Organizer
  第71回日本生物工学会大会シンポジウム「産業化を目指した生体分子工学の新たな潮流」, 岡山大学津島キャンパス
- Invited
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] 小さく、高発現・高輝度なALucの作製2019
- Author(s)
  大室有紀・金誠培・上田宏
- Organizer
  生物発光化学発光研究会第35回学術講演会
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] Development of ternary protein-protein interaction assay using peptide tags made from NanoLuc.2018
- Author(s)
  大室　有紀
- Organizer
  第18回日本蛋白質科学会年会
- Invited
- Data Source
  KAKENHI-PROJECT-17K06920
[Presentation] 蛍光ラベルを要しないクエンチ抗体構築法の開発2018
- Author(s)
  泉谷篤史, 安田貴信, 大室有紀, 北口哲也, 上田宏
- Organizer
  第91回日本生化学会大会
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] Creation of simple artificial cell-based biosensor that detects tag-specific antibody2018
- Author(s)
  Jiulong SU, Cyrus BEH, Tetsuya KITAGUCHI, Yuki OHMURO, Theresa SEAH, Shawn HOON, Hiroshi UEDA
- Organizer
  酵素工学研究会第80回講演会
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] Thermostabilization of mutant beta-glucuronidase resulted in increased stability and lower background signal of homogeneous immunoassay2018
- Author(s)
  Jiulong Su, Yuki Omuro, Hiroshi Ueda
- Organizer
  第18回日本蛋白質科学会年会
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] Mutant firefly luciferase with enhanced oxidative luminescent activity improved the response of protein-protein interaction assay FlimPIA2018
- Author(s)
  Hiroshi Ueda, Yuki Ohmuro
- Organizer
  International Symposium on Bioluminescence and Chemiluminescence
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K06920
[Presentation] 均相系測定を可能にする抗原応答性標識抗体の創製2018
- Author(s)
  上田宏, 大室有紀, 北口哲也
- Organizer
  日本分析化学会第67年会
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] 発光酵素を用いたタンパク質相互作用検出系2018
- Author(s)
  大室有紀
- Organizer
  LiHUB第5回創薬塾「創薬を目指した分子デザイン」
- Invited
- Data Source
  KAKENHI-PROJECT-17K06920
[Presentation] Homogeneous noncompetitive immunological detection of small molecules by ternary luciferase fragment complementation (OS-BLIA)2018
- Author(s)
  Hiroshi Ueda, Yuki Ohmuro
- Organizer
  20th International Symposium on Bioluminescence and Chemiluminsecence
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] Construction of luminescent immunosensors using luciferase-fused Quenchbody2018
- Author(s)
  Riho Takahashi, Yuki Ohmuro, Hiroshi Ueda
- Organizer
  20th International Symposium on Bioluminescence and Chemiluminsecence
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] 発光酵素を用いたタンパク質相互作用検出系FlimPIAとストレプトアビジンビーズを用いた相互作用検出系の構築2018
- Author(s)
  大室有紀・五味恵子・山地秀樹・上田宏
- Organizer
  生物発光化学発光研究会第34回学術講演会
- Data Source
  KAKENHI-PROJECT-17K06920
[Presentation] Novel Protein-Protein Interaction Assay Based on the Functional Complementation of Mutant Firefly Luciferases: Split Structure Versus Divided Reaction2018
- Author(s)
  Yuki Ohmuro, Hiroshi Ueda
- Organizer
  第18回日本蛋白質科学会年会
- Invited
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] Homogeneous noncompetitive immunological detection of small molecules by ternary luciferase fragment complementation (OS-BLIA2018
- Author(s)
  Hiroshi Ueda, Yuki Ohmuro
- Organizer
  International Symposium on Bioluminescence and Chemiluminescence
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K06920
[Presentation] Creation of simple artificial cell-based biosensor that detects tag-specific antibody2018
- Author(s)
  Jiulong SU, Cyrus BEH, Tetsuya KITAGUCHI, Yuki OHMURO, Theresa SEAH, Shawn HOON, Hiroshi UEDA
- Organizer
  第91回日本生化学会大会
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] Mutant firefly luciferase with enhanced oxidative luminescent activity improved the response of protein-protein interaction assay FlimPIA2018
- Author(s)
  Yuki Ohmuro, Hiroshi Ueda
- Organizer
  20th International Symposium on Bioluminescence and Chemiluminsecence
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] Thermostabilization of mutant beta-glucuronidase resulted in increased stability and lower background signal of homogeneous immunoassay2018
- Author(s)
  Jiulong Su, Yuki Omuro, Hiroshi Ueda
- Organizer
  15th Japan-China-Korea Joint Symposium on Enzyme Engineering
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] ラクダ科動物由来重鎖抗体を用いた蛍光免疫センサーmini Q-bodyの構築2018
- Author(s)
  上田宏, 山田映未, 井上暁人, 大室有紀, 北口哲也
- Organizer
  2018年度日本生物工学会大会
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] 自ら光るクエンチ抗体BRET Q-bodyの発光特性2018
- Author(s)
  高橋里帆, 大室有紀, 上田宏
- Organizer
  第12回バイオ関連化学シンポジウム
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] 高性能な蛍光バイオセンサー構築のための抗体結合プローブの開発2018
- Author(s)
  上田宏, 山田映未, 井上暁人, 大室有紀, 北口哲也
- Organizer
  2018年度日本生物工学会大会
- Data Source
  KAKENHI-PROJECT-18H03851
[Presentation] A Novel Protein-Protein Interaction Assay Based on the Functional Complementation of Mutant Firefly Luciferases: Split Structure Versus Divided Reaction2018
- Author(s)
  Yuki Omuro, Hiroshi Ueda
- Organizer
  第18回日本蛋白質科学会年会
- Invited
- Data Source
  KAKENHI-PROJECT-17K06920
[Presentation] Mutant firefly luciferase with enhanced oxidative luminescent activity improved the response of protein-protein interaction assay FlimPIA2018
- Author(s)
  大室　有紀・上田　宏
- Organizer
  ISBC2018
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K06920
[Presentation] ストレプトアビジンビーズを用いたタンパク質間相互作用検出系FlimPIAの応用2018
- Author(s)
  大室有紀・五味恵子・山地秀樹・上田宏
- Organizer
  酵素工学研究会第80回講演会
- Data Source
  KAKENHI-PROJECT-17K06920
[Presentation] 生物発光共鳴エネルギー移動を利用した蛋白質認識抗体プローブの開発2017
- Author(s)
  田中絵美理, 鍾蝉伊, 大室有紀, 董金華, 上田宏
- Organizer
  化学工学会第82年会
- Data Source
  KAKENHI-PROJECT-15H04191
[Presentation] 蛋白質間相互作用検出系FlimPIAにおける発光値の向上2017
- Author(s)
  大室有紀・上田宏
- Organizer
  酵素工学研究会第78回講演会
- Data Source
  KAKENHI-PROJECT-17K06920
[Presentation] DEVELOPMENT OF RAPID IMMUNOASSSAY USING NANOLUC-DERIVED PEPTIDE TAGS2017
- Author(s)
  大室　有紀・上田　宏
- Organizer
  生物発光化学発光研究会第33回学術講演会
- Data Source
  KAKENHI-PROJECT-17K06920
[Presentation] 発光酵素融合クエンチ抗体を用いたBRET抗原検出系の構築2017
- Author(s)
  高橋里帆, 大室有紀, 上田宏
- Organizer
  第17回日本蛋白質科学会年会
- Data Source
  KAKENHI-PROJECT-15H04191
[Presentation] DEVELOPMENT OF RAPID IMMUNOASSSAY USING NANOLUC-DERIVED PEPTIDE TAGS2017
- Author(s)
  大室有紀・上田宏
- Organizer
  Enzyme Engineering XXIV
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K06920
[Presentation] リチウム添加による昆虫細胞からの抗体分泌生産の向上2017
- Author(s)
  大室有紀, 上田宏, 山地秀樹
- Organizer
  化学工学会第82年会
- Data Source
  KAKENHI-PROJECT-15H04191
[Presentation] NanoLuc由来ペプチドタグを用いた簡便迅速な免疫測定法の開発2017
- Author(s)
  大室有紀・蘇九龍・高橋里帆・上田宏
- Organizer
  第69回日本生物工学会大会
- Data Source
  KAKENHI-PROJECT-17K06920
[Presentation] 発光酵素融合クエンチ抗体を用いた発光免疫センサーの構築2017
- Author(s)
  高橋里帆, 大室有紀, 上田宏
- Organizer
  日本化学会第11回バイオ関連シンポジウム
- Data Source
  KAKENHI-PROJECT-15H04191
[Presentation] 天然抗体をQuenchbody化するための抗体結合蛍光プローブの開発2017
- Author(s)
  三宅千絢, 鄭煕陳, 大室有紀, 董金華, 上田宏
- Organizer
  化学工学会第82年会
- Data Source
  KAKENHI-PROJECT-15H04191
[Presentation] 発光酵素融合クエンチ抗体を用いた生物発光抗原検出系の構築2017
- Author(s)
  高橋里帆, 大室有紀, 上田宏
- Organizer
  日本生物工学会大会
- Data Source
  KAKENHI-PROJECT-15H04191
[Presentation] ３分子間PCA原理に基づく非競合生物発光免疫測定系の構築2017
- Author(s)
  大室有紀, 上田　宏
- Organizer
  ConBio2017
- Data Source
  KAKENHI-PROJECT-15H04191
[Presentation] ラクダ科動物由来重鎖抗体を用いたQ-bodyの構築2017
- Author(s)
  山田映未，岡裕也，大室有紀，董金華，上田宏
- Organizer
  第17回日本蛋白質科学会年会
- Data Source
  KAKENHI-PROJECT-15H04191
[Presentation] Development of rapid bioluminescent immunoassay using Nanoluc-derived peptide tags2017
- Author(s)
  大室有紀・蘇九龍・上田宏
- Organizer
  生物発光化学発光研究会第33回学術講演会
- Data Source
  KAKENHI-PROJECT-17K06920
[Presentation] 血清アルブミン検出のための生物発光抗体プローブの開発2016
- Author(s)
  田中絵美理, 鐘蝉伊, 大室有紀, 上田宏
- Organizer
  第89回日本生化学会大会
- Data Source
  KAKENHI-PROJECT-15H04191
[Presentation] ホタル発光酵素を用いたタンパク質間相互作用検出系の数学モデル2016
- Author(s)
  上田宏 , Dale Renee , 大室有紀 , 加藤直洋
- Organizer
  化学工学会第４８回秋季大会
- Data Source
  KAKENHI-PROJECT-15H04191
[Presentation] Hinge region mutagenesis of beetle luciferases for the improved protein-protein interaction assay FlimPIA2016
- Author(s)
  Hiroshi Ueda, Hideki Yamaji, Yuki Ohmuro-Matsuyama
- Organizer
  International Conference on Bioluminescence and Chemiluminescence (ISBC)2018
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15H04191
[Presentation] Hinge region engineering of firefly luciferases for robust protein-protein interaction assay FlimPIA2015
- Author(s)
  Hiroshi Ueda, Hideki Yamaji, Yuki Ohmuro-Matsuyama
- Organizer
  Pacifichem 2015
- Place of Presentation
  Sheraton Waikiki, Honolulu
- Year and Date
  2015-12-16
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15H04191
[Presentation] Detection of vimentin serine phosphorylation by Quenchbodies2011
- Author(s)
  Hee-Jin Jeong, Hiroyuki Ohashi, Yuki Ohmuro-Matsuyama, Masaki Inagaki and Hiroshi Ueda
- Organizer
  AsiaSense 2011 (http://www.asiasense2011.org/)
- Place of Presentation
  済州, 韓国
- Data Source
  KAKENHI-PROJECT-23656517
[Presentation] オープンサンドイッチ原理と新規相互作用検出系による蛋白質リン酸化修飾の検出2011
- Author(s)
  大室(松山)有紀, 北岡優一, ジョンヒジン, 達吉郎, 稲垣昌樹, 上田宏
- Organizer
  第11回蛋白質科学会年会2WB-3
- Place of Presentation
  ホテル阪急エキスポパーク(招待講演)
- Year and Date
  2011-06-07
- Data Source
  KAKENHI-PUBLICLY-22019007
[Presentation] オープンサンドイッチ原理と新規相互作用検出系による蛋白質リン酸化修飾の検出2011
- Author(s)
  大室（松山）有紀，北岡優一，ジョンヒジン，達吉郎，稲垣昌樹，上田　宏
- Organizer
  第11回蛋白質科学会年会 2WB-3(招待講演)
- Place of Presentation
  ホテル阪急エキスポパーク
- Data Source
  KAKENHI-PROJECT-23656517
[Presentation] Detection of vimentin serine phosphorylation by Quenchbodies2011
- Author(s)
  Hee-Jin Jeong, Hiroyuki Ohashi, Yuki Ohmuro-Matsuyama, Masaki Inagaki, Hiroshi Ueda
- Organizer
  AsiaSense 2011 (http://www.asiasense2011.org/)
- Place of Presentation
  済州,韓国(Best Poster Award受賞)
- Year and Date
  2011-10-26
- Data Source
  KAKENHI-PUBLICLY-22019007
[Presentation] オープンサンドイッチ原理と新規相互作用検出系による蛋白質リン酸化修飾の検出2011
- Author(s)
  大室(松山)有紀,北岡優一,ジョンヒジン,達吉郎,稲垣昌樹,上田宏
- Organizer
  第11回蛋白質科学会年会
- Place of Presentation
  大阪
- Year and Date
  2011-06-08
- Data Source
  KAKENHI-PROJECT-23656517
[Presentation] Detection of vimentin serine phosphorylation by Quenchbodies2011
- Author(s)
  Hee-Jin Jeong, Hiroyuki Ohashi, Yuki Ohmuro- Matsuyama Masaki Inagaki and Hiroshi Ueda
- Organizer
  AsiaSense 2011
- URL
  http://www.asiasense2011.org/
- Place of Presentation
  済州,韓国
- Year and Date
  2011-10-26
- Data Source
  KAKENHI-PROJECT-23656517
[Presentation] Functional Complementation of Luciferase Half-Reactions: Application to a Robust Protein-Protein Interaction Assay FlimPIA
- Author(s)
  Hiroshi Ueda, Takahiro Yamashita, and Yuki Ohmuro-Matsuyama
- Organizer
  Active Enzyme Molecules 2014
- Place of Presentation
  富山国際会議場
- Year and Date
  2014-12-17 – 2014-12-19
- Data Source
  KAKENHI-PROJECT-24360336

1. UEDA Hiroshi (60232758)

# of Collaborated Projects: 6 results

# of Collaborated Products: 65 results
2. DONG Jinhua (80527838)

# of Collaborated Projects: 2 results

# of Collaborated Products: 6 results
3. OHASHI Hiroyuki (40589454)

# of Collaborated Projects: 1 results

# of Collaborated Products: 3 results
4. KOBAYASHI Norihiro (90205477)

# of Collaborated Projects: 1 results

# of Collaborated Products: 1 results
5. 鄭煕陳 (70737981)

# of Collaborated Projects: 1 results

# of Collaborated Products: 3 results
6. ABE Ryoji

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
7. ITO YUMA

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
8. OYAMA Hiroyuki

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
9. 上野隆史

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
10. 森岡弘志

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
11. 木村宏

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
12. 森田いずみ

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
13. 中村浩之

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
14. 原田一貴

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results

Yuki Ohmuro 大室 有紀

松山有紀

MATSUYAMA Yuki 松山 有紀

OHMURO Yuki

Research Projects

Research Products

Co-Researchers

New development in fluorescent immunosensor based on the quench-release principle by protein and peptide engineering

Principal Investigator

Project Period (FY)

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Research Institution

Development of sensitive multi-colour detection using the protein-protein interaction assay, FlimPIAPrincipal Investigator

Principal Investigator

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Research Institution

Development of innovative immunoassay through the application of quench-release principle to natural antibodies

Principal Investigator

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Research Institution

Creation of Ultra Super Fusion Protein for Antibody Binding and Purification

Principal Investigator

Project Period (FY)

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Novel Expansion of Bioanalysis Based on the Fluorescence Quench-Release Principle

Principal Investigator

Project Period (FY)

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Research Institution

迅速・高感度な多色細胞内マルチ蛋白質間相互作用検出系の構築Principal Investigator

Principal Investigator

Project Period (FY)

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Research Institution

Construction of versatile fluoroimmunoassay probe based on quench-release principle

Principal Investigator

Project Period (FY)

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Research Institution

抗リン酸化ペプチド抗体を用いた細胞周期制御機構の可視化

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

[Book] Protein-Protein Interaction Assays Using Split-NanoLuc; in Bioluminescence - Analytical Applications and Basic Biology2019

Author(s)

Total Pages

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ISBN

Data Source

[Book] 発光イメージング実験ガイド2019

Author(s)

Total Pages

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ISBN

Data Source

[Book] 分割NanoLucを用いた相互作用検出系による低分子の免疫測定; in 実験医学別冊 発光イメージング実験ガイド (Eds. 永井健治・小澤岳昌）, 194-1972019

Author(s)

Total Pages

Publisher

ISBN

Data Source

[Book] Bioluminescence2019

Author(s)

Total Pages

Publisher

Data Source

[Book] Protein-Protein Interaction Assays2018

Author(s)

Yuki Ohmuro 大室有紀

MATSUYAMA Yuki 松山有紀

[Book] 分割NanoLucを用いた相互作用検出系による低分子の免疫測定; in 実験医学別冊　発光イメージング実験ガイド (Eds. 永井健治・小澤岳昌）, 194-1972019