CHIHARA Yoshitomo 千原良友

… Alternative Names

Researcher Number	40405395
Affiliation (based on the past Project Information) *help	2017 – 2019: 奈良県立医科大学, 医学部附属病院, 研究員 2014 – 2016: 奈良県立医科大学, 医学部, 研究員 2010 – 2015: 奈良県立医科大学, 医学部, 講師 2007: 奈良県立医大, 医学部附属病院, 研究員 2006: 奈良県立医科大学, 医学部, 博士研究員
Review Section/Research Field	Principal Investigator Urology Except Principal Investigator Human pathology / Urology
Keywords	Principal Investigator DNAメチル化 / 尿中剥離細胞 / エピジェネティクス / 表在性膀胱癌 / マッピング解析 / 染色体欠失 / ホモ欠失 / DNAチップ / 腫瘍学 / 膀胱がん … More Except Principal Investigator がん幹細胞 / DNAメチル化 / ミトコンドリア / がん微小環境 / 骨髄間葉系幹細胞 / 大腸癌 / 肝転移 / 脂肪酸 / 癌幹細胞 / カロリー制限 / TGFβ / HMGB1 / リノール酸 / エライジン酸 / 糖尿病 / リジン・カルボキシメチル化 / ヒストン・アセチル化 / 糖化 / 糖化最終産物 / ヒストン / 遺伝子発現 / リジンカルボキシメチル化 / H3ヒストンK27 / トリメチル化 / ヒストン・リジン修飾 / カルボキシメチル化 / エピジェネティクス / p16 / H4ヒストン / リジン残基 / アセチル化ヒストン / 多発性嚢胞腎 / ＰＣＫラット / 細胞マトリックス / ベンゾイソフラノン誘導体 / ラットＰＣＫ / 細胞外マトリクス / ＤＮＡメチル化 / ベンゾイソフラノン化合物 / 幹細胞分化 / キネティクス / がん細胞分化・脱分化 / 多光子励起蛍光顕微鏡 / 冬眠性がん幹細胞 / 増殖性がん幹細胞 / 分化がん細胞 / 幹細胞可塑性 / 膀胱癌 / 上皮内癌 / 膀胱内注入療法 / CLDN / CXCL1 / Collagen / CD56 / 制御性T細胞 / 膀胱がん / 免疫細胞 / アミノレブリン酸 / BCG / コラーゲン / 鉄 / 腫瘍微小環境 / 膀胱上皮内癌 / 上皮間葉転換 / 血管新生因子 / 免疫応答 / クローディン / タイトジャンクション / 抗癌剤 / 分子標的薬 / 細菌 / 上皮間葉移行 / 幹細胞性 / クローディン４ / YAP / Hippo / CPE / clostridium perfringens / 抗がん剤併用療法 / 抗がん剤耐性 / 抗がん剤副作用 / タンネリング / ナノチューブ / 薬剤耐性 / 抗Claudin抗体 / 化学療法増感 / Claudinシグナル / 副作用 / 間葉系幹細胞 / 細胞間相互作用 / 癌転移 / ヘテロスフェア / claudin / 抗体療法 / tight junction / 抗がん剤増感 / 抗クローディン4抗体 / 胃癌 / ピロリ菌感染 Less

Application of anti-claudin-4 antibody to anti-cancer therapy
- Principal Investigator
  
  Kuniyasu Hiroki
- Project Period (FY)
  2016 – 2019
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  Human pathology
- Research Institution
  Nara Medical University
Molecular biological profile of carcinoma in situ of urinary bladder as predictive and prognostic markers
- Principal Investigator
  
  FUJIMOTO KIYOHIDE
- Project Period (FY)
  2015 – 2017
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Urology
- Research Institution
  Nara Medical University
Aberrant DNA methylation of cancer stem cell genes correlate with bladder carcinogenesisPrincipal Investigator
- Principal Investigator
  
  Chihara Yoshitomo
- Project Period (FY)
  2014 – 2017
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Urology
- Research Institution
  Nara Medical University
In vivo observation of stemness acquisition in differentiated cancer cells
- Principal Investigator
  
  KUNIYASU Hiroki
- Project Period (FY)
  2013 – 2014
- Research Category
  
  Grant-in-Aid for Challenging Exploratory Research
- Research Field
  
  Human pathology
- Research Institution
  Nara Medical University
Novel epigenetic change by histone lysine carboxymethylation
- Principal Investigator
  
  Fujii Kiyomu
- Project Period (FY)
  2012 – 2015
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Human pathology
- Research Institution
  Nara Medical University
Basic studies of therapeutic modality for polycystic kidney disease explored by epigenetic modification of cellular matrix-adhesion proteins
- Principal Investigator
  
  ISHIBASHI MIchio
- Project Period (FY)
  2012 – 2014
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Urology
- Research Institution
  Nara Medical University
Targeting of mesenchymal stem cell associated with colorectal cancer liver metastasis
- Principal Investigator
  
  OHMORI Hitoshi
- Project Period (FY)
  2011 – 2013
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Human pathology
- Research Institution
  Nara Medical University
Diagnostic markers of urothelial cancer based on DNA methylation analysisPrincipal Investigator
- Principal Investigator
  
  CHIHARA Yoshitomo
- Project Period (FY)
  2010 – 2011
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Urology
- Research Institution
  Nara Medical University
表在性膀胱癌における全Genome領域のAllelotypingとホモ欠失の解析Principal Investigator
- Principal Investigator
  
  千原良友
- Project Period (FY)
  2006 – 2007
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Urology
- Research Institution
  Nara Medical University

All 2017 2016 2015 2014 2013 2012 2011 2010 Other

All Journal Article Presentation

[Journal Article] Diagnostic and prognostic role of urinary collagens in primary human bladder cancer2017
- Author(s)
  Miyake M, Morizawa Y, Hori S, Tatsumi Y, Onishi S, Owari T, Iida K, Onishi K, Gotoh D, Nakai Y, Anai S, Chihara Y, Torimoto K, Aoki K, Tanaka N, Shimada K, Konishi N, Fujimoto K.
- Journal Title
  
  Cancer Sci
  
  Volume: 108 Pages: 2221-2228
- DOI
  10.1111/cas.13384
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-15K10605, KAKENHI-PROJECT-16K20159
[Journal Article] Expression of ferrochelatase has a strong correlation in protoporphyrin IX accumulation with photodynamic detection of bladder cancer.2016
- Author(s)
  Yasushi Nakai, Yoshihiro Tatsumi, Makito Miyake, Satoshi Anai, Masaomi Kuwada, Sayuri Onishi, Yoshitomo Chihara, Nobumichi Tanaka, Yoshihiko Hirao, Kiyohide Fujimoto
- Journal Title
  
  Photodiagnosis Photodyn Ther
  
  Volume: 13 Pages: 225-232
- DOI
  10.1016/j.pdpdt.2015.07.174
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-15K10605
[Journal Article] Pro-chemotherapeutic effects of antibody against extracellular domain of claudin-4 in bladder cancer2015
- Author(s)
  Masaomi Kuwada, Yoshitomo Chihara, Yi Luo, Xiangru Li, Yukiko Nishiguchi, Rina Fujiwara, Takamistu Sasaki, Kiyomu Fujii, Hitoshi Ohmori, Kiyohide Fujimoto, Masuo Kondoh, Hiroki Kuniyasu
- Journal Title
  
  Cancer Lett
  
  Volume: 369 Pages: 212-221
- DOI
  10.1016/j.canlet.2015.08.019
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-15K10605, KAKENHI-PROJECT-26460424, KAKENHI-PROJECT-24590450
[Journal Article] Expression of MAS1 in breast cancer.2015
- Author(s)
  Luo Y, Tanabe E, Kitayoshi M, Nishiguchi Y, Fujiwara R, Matsushima S, Sasaki T, Sasahira T, Chihara Y, Fujii K, Ohmori H, Kuniyasu H
- Journal Title
  
  Cancer Sci.
  
  Volume: 106 Pages: 1240-1248
- DOI
  10.1111/cas.12719
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-26460424, KAKENHI-PROJECT-24590450
[Journal Article] Cancer usurps skeletal muscle as an energy repository.2014
- Author(s)
  Luo Y, Ohmori H, et al
- Journal Title
  
  Cancer Research
  
  Volume: 74 Pages: 330-340
- DOI
  10.1158/0008-5472.can-13-1052
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-23590430, KAKENHI-PROJECT-25670177, KAKENHI-PROJECT-26460424
[Journal Article] Novel missense mutation in the FH gene in familial renal cell cancer patients lacking cutaneous leiomyomas.2014
- Author(s)
  Kuwada M, Kuniyasu H, et al
- Journal Title
  
  BMC Research Notes
  
  Volume: 7 Pages: 203-203
- DOI
  10.1186/1756-0500-7-203
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-25670177
[Journal Article] High mobility group box 1 released from necrotic cells enhances regrowth and metastasis of cancer cells that have survived chemotherapy2013
- Author(s)
  Luo Y, Chihara Y, Fujimoto K, Sasahira T, Kuwada M, Fujiwara R, Fujii K, Ohmori H, Kuniyasu H
- Journal Title
  
  Eur J Cancer
  
  Volume: 49 Pages: 741-751
- DOI
  10.1016/j.ejca.2012.09.016
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23590430, KAKENHI-PROJECT-23659188, KAKENHI-PROJECT-24590450, KAKENHI-PROJECT-25670177
[Journal Article] Diagnostic markers of urothelial cancer based on DNA methylation analysis.2013
- Author(s)
  Chihara Y, Kuniyasu H, et al.
- Journal Title
  
  BMC Cancer
  
  Volume: 13 Pages: 275-275
- DOI
  10.1186/1471-2407-13-275
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-25670177
[Journal Article] High matrix metalloproteinase-to-E-cadherin ratio measured by bicolor fluorescent in situ hybridization is associated with lymphangiogenesis and lymph node metastasis in prostate cancer2012
- Author(s)
  Luo Y, Ohmori H, Fujii K, Chihara Y, Maruyama S, Kuniyasu H
- Journal Title
  
  Urol Oncol-Semin Ori
  
  Volume: 30 Pages: 306-313
- DOI
  10.1016/j.urolonc.2010.05.001
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23590430, KAKENHI-PROJECT-23659188, KAKENHI-PROJECT-24590450
[Journal Article] Advanced glycation end products (AGE) induce the receptor for AGE in the colonic mucosa of azoxymethane-injected Fischer 344 rats fed with a high-linoleic acid and high-glucose diet.2012
- Author(s)
  Shimomoto T, Luo Y, Ohmori H, Chihara Y, Sasahira T, Fujii K, Kuniyasu H
- Journal Title
  
  J Gastroenterol
  
  Volume: 47 Pages: 1073-83
- DOI
  10.1007/s00535-012-0572-5
- NAID
  10031122095
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23590430, KAKENHI-PROJECT-23659188, KAKENHI-PROJECT-24590450
[Journal Article] Diabetes-associated angiotensin activation enhances liver metastasis of colon cancer.2012
- Author(s)
  Shimomoto T, Ohmori H, Luo Y, Chihara Y, Denda A, Sasahira T, Tatsumoto N, Fujii K, Kuniyasu H
- Journal Title
  
  Clin Exp Metasta
  
  Volume: 29 Pages: 915-925
- DOI
  10.1007/s10585-012-9480-6
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23590430, KAKENHI-PROJECT-23659188, KAKENHI-PROJECT-24590450
[Presentation] 抗claudin-4抗体の併用による抗体分子治療薬の効果促進2016
- Author(s)
  國安弘基、羅　奕、桑田真臣、佐々木隆光、千原良友、藤井　澄、大森　斉
- Organizer
  第105回日本病理学会総会
- Place of Presentation
  仙台市
- Year and Date
  2016-05-12
- Data Source
  KAKENHI-PROJECT-16H05164
[Presentation] Enhancement of antitumoral effect of molecular-targeting antibody by anti-claudin-4 antibody.2016
- Author(s)
  Kuniyasu H, Luo Y, Sasaki T, Chihara Y, Fujiwara R, Nishiguchi Y, Hashimoto Y, Kondo M
- Organizer
  76th Annual Meeting of Japanese Association for Cancer
- Place of Presentation
  横浜市
- Year and Date
  2016-10-06
- Data Source
  KAKENHI-PROJECT-16H05164
[Presentation] Pro-chemotherapeutic effects of antibody against extracellular domain of claudin-4 in bladder cancer.2016
- Author(s)
  Kuwada M, Chihara Y, Luo Y, Sasaki T, Fujimoto K, Kondoh M, Kuniyasu H
- Organizer
  Annual Meeting of American Association for Cancer Research 2016
- Place of Presentation
  New Orleans, USA
- Year and Date
  2016-04-16
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16H05164
[Presentation] Cancer stem-like cells and epithelial mesenchymal transition is responsible for failure in bacillus Calmette-Guérin therapy for primary carcinoma in situ.2015
- Author(s)
  Kuwada M, Chihara Y, Miyake M, Anai S, Tanaka N, Kuniyasu H, Fujimoto K
- Organizer
  Annual Meeting of American Urology Association 2015
- Place of Presentation
  New Orleans
- Year and Date
  2015-05-15
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-24590450
[Presentation] Claudine-4 細胞外ドメインを認識するモノクロナル抗体による膀胱癌の分子標的療法の検討2015
- Author(s)
  桑田真臣、羅　奕、李　相儒、西口由希子、藤原里奈、佐々木隆光、千原良友、藤本清秀、近藤昌夫、國安弘基
- Organizer
  第24回日本がん転移学会学術集会・総会
- Place of Presentation
  シティプラザ大阪
- Year and Date
  2015-07-23
- Data Source
  KAKENHI-PROJECT-15K10605
[Presentation] アンギオテンシン系および血糖調節を標的とする大腸癌肝転移の抑制2012
- Author(s)
  大森　斉、羅　奕、川田元清、千原良友、國安弘基
- Organizer
  第101回日本病理学会総会
- Place of Presentation
  東京
- Data Source
  KAKENHI-PROJECT-23590430
[Presentation] トランス脂肪酸によるEGFR活性化2012
- Author(s)
  羅　奕、藤井　澄、森若優希子、千原良友、國安弘基
- Organizer
  第101回日本病理学会総会
- Place of Presentation
  大津
- Data Source
  KAKENHI-PROJECT-24590450
[Presentation] 大腸がんにおける宿主代謝への影響2012
- Author(s)
  國安弘基、羅　奕、栗原　都、笹平智則、千原良友、藤井　澄、大森　斉
- Organizer
  第27 回発癌病理研究会、シンポジウム5
- Place of Presentation
  修善寺
- Data Source
  KAKENHI-PROJECT-24590450
[Presentation] Angiotensin in colorectal cancer metastasis2012
- Author(s)
  Luo Y, Chihara Y, Ohmori H, Fujii K, Yoshikawa M, Kuwata M, Fujimoto K, Hirao Y, Kitadai Y, Kuniyasu H
- Organizer
  103rd Annual Meeting of American Association for Cancer Research
- Place of Presentation
  Chicago
- Data Source
  KAKENHI-PROJECT-24590450
[Presentation] Role of myxomatous stroma to mesenchymal stem cells in colorectal cancer2012
- Author(s)
  Kuniyasu H, Fujimoto R, Luo Y, Fujii K, Ohmori H, Maruyama H, Sasahira T, Chihara Y
- Organizer
  71st Annual Meeting of Japanese Cancer Association
- Place of Presentation
  札幌
- Data Source
  KAKENHI-PROJECT-23590430
[Presentation] 大腸がんにおける宿主代謝への影響2012
- Author(s)
  國安弘基、羅　奕、栗原　都、笹平智則、千原良友、藤井　澄、大森　斉
- Organizer
  第27 回発癌病理研究会、シンポジウム5
- Place of Presentation
  修善寺
- Data Source
  KAKENHI-PROJECT-23590430
[Presentation] トランス脂肪酸によるEGFRのトランス活性化2012
- Author(s)
  藤井　澄、羅　奕、桑田真臣、森若優希子、千原良友、藤本清秀、國安弘基
- Organizer
  第21回日本がん転移学会
- Place of Presentation
  広島
- Data Source
  KAKENHI-PROJECT-24590450
[Presentation] Effect of fatty acids oral intake on tumor stem cells2012
- Author(s)
  Ohmori H, Katada N, Shima K, Luo Y, Kurihara M, Chihara Y, Kuniyasu H
- Organizer
  71st Annual Meeting of Japanese Cancer Association
- Place of Presentation
  札幌
- Data Source
  KAKENHI-PROJECT-23590430
[Presentation] 尿中DNAメチル化異常を指標とした尿路上皮がん診断2011
- Author(s)
  千原良友、金井弥栄、藤本博行
- Organizer
  日本泌尿器科学会総会
- Place of Presentation
  名古屋
- Year and Date
  2011-04-21
- Data Source
  KAKENHI-PROJECT-22791508
[Presentation] Diagnostic indicator of urothelial cancer based on DNA methylation analysis2011
- Author(s)
  Chihara Y, Kuniyasu H, Liang G, et al.
- Organizer
  American Urological Association Annual meeting
- Place of Presentation
  ワシントンDC
- Year and Date
  2011-05-15
- Data Source
  KAKENHI-PROJECT-22791508
[Presentation] Diagnostic indicator of urothelial cancer based on DNA methylation analysis2011
- Author(s)
  Chihara Yoshitomo
- Organizer
  American Urological Assosiation Annual Meeting
- Place of Presentation
  Washington DC
- Year and Date
  2011-05-15
- Data Source
  KAKENHI-PROJECT-22791508
[Presentation] 高血糖の大腸癌肝転移に及ぼす影響2011
- Author(s)
  大森　斉、羅　奕、藤井　澄、下元貴澄、千原良友、國安弘基
- Organizer
  第100回日本病理学会
- Place of Presentation
  横浜市
- Data Source
  KAKENHI-PROJECT-23590430
[Presentation] Two molecular pathways to bladder cancer involve opposing roles of DNA methylation2010
- Author(s)
  千原良友
- Organizer
  日本癌学会学術集会
- Place of Presentation
  大阪
- Year and Date
  2010-09-23
- Data Source
  KAKENHI-PROJECT-22791508
[Presentation] Two molecular pathways to bladder cancer involve opposing roles of DNA methylation2010
- Author(s)
  千原良友
- Organizer
  日本癌学会学術総会
- Place of Presentation
  大阪国際会議場
- Year and Date
  2010-09-24
- Data Source
  KAKENHI-PROJECT-22791508
[Presentation] Claudin-4を標的とする治療の可能性
- Author(s)
  國安弘基、羅　奕、千原良友、桑田真臣、北吉美沙穂、田邊絵里子、佐々木隆光、大森　斉、近藤昌夫
- Organizer
  第29回発癌病理研究会
- Place of Presentation
  いわき市
- Year and Date
  2014-09-01 – 2014-09-03
- Data Source
  KAKENHI-PROJECT-25670177

1. KUNIYASU Hiroki (00253055)

# of Collaborated Projects: 4 results

# of Collaborated Products: 0 results
2. KITADAI Yasuhiko (10304437)

# of Collaborated Projects: 3 results

# of Collaborated Products: 0 results
3. OHMORI Hitoshi (80213875)

# of Collaborated Projects: 2 results

# of Collaborated Products: 13 results
4. FUJII Kiyomu (60284189)

# of Collaborated Projects: 2 results

# of Collaborated Products: 13 results
5. SASSAKI Takamitsu (00382284)

# of Collaborated Projects: 2 results

# of Collaborated Products: 3 results
6. FUJIMOTO KIYOHIDE (50264867)

# of Collaborated Projects: 2 results

# of Collaborated Products: 4 results
7. SASAHIRA Tomonori (90405374)

# of Collaborated Projects: 1 results

# of Collaborated Products: 5 results
8. ISHIBASHI MIchio (40107032)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
9. HIGASHIHARA Eiji (00092312)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
10. NAGAO Shizuko (20183527)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
11. KOJIMA Naoto (90420413)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
12. FUJIMOTO Yoshio (90192731)

# of Collaborated Projects: 1 results

# of Collaborated Products: 1 results
13. TAKAKI Miyako (00033358)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
14. 三宅牧人 (80601400)

# of Collaborated Projects: 1 results

# of Collaborated Products: 2 results
15. 国安弘基

# of Collaborated Projects: 0 results

# of Collaborated Products: 23 results

CHIHARA Yoshitomo 千原 良友

千原 良友 チハラ ヨシトモ

Research Projects

Research Products

Co-Researchers

Application of anti-claudin-4 antibody to anti-cancer therapy

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Molecular biological profile of carcinoma in situ of urinary bladder as predictive and prognostic markers

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Aberrant DNA methylation of cancer stem cell genes correlate with bladder carcinogenesisPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

In vivo observation of stemness acquisition in differentiated cancer cells

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Novel epigenetic change by histone lysine carboxymethylation

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Basic studies of therapeutic modality for polycystic kidney disease explored by epigenetic modification of cellular matrix-adhesion proteins

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Targeting of mesenchymal stem cell associated with colorectal cancer liver metastasis

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Diagnostic markers of urothelial cancer based on DNA methylation analysisPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

表在性膀胱癌における全Genome領域のAllelotypingとホモ欠失の解析Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

[Journal Article] Diagnostic and prognostic role of urinary collagens in primary human bladder cancer2017

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Expression of ferrochelatase has a strong correlation in protoporphyrin IX accumulation with photodynamic detection of bladder cancer.2016

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Pro-chemotherapeutic effects of antibody against extracellular domain of claudin-4 in bladder cancer2015

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Expression of MAS1 in breast cancer.2015

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Cancer usurps skeletal muscle as an energy repository.2014

CHIHARA Yoshitomo 千原良友

千原良友チハラヨシトモ

[Presentation] Cancer stem-like cells and epithelial mesenchymal transition is responsible for failure in bacillus Calmette-Guérin therapy for primary carcinoma in situ.2015