Yoshikawa Hiroyuki 吉川廣幸

Researcher Number	40733936
Other IDs	https://orcid.org/0000-0002-6673-7924
Affiliation (Current)	2025: リージョナルフィッシュ株式会社(研究開発部), 研究開発部, 主席研究員
Affiliation (based on the past Project Information) *help	2021 – 2024: 国立研究開発法人水産研究・教育機構, 水産大学校, 講師 2018 – 2020: 国立研究開発法人水産研究・教育機構, 水産大学校, 助教 2014 – 2015: 独立行政法人水産大学校, その他部局等, 助教
Review Section/Research Field	Principal Investigator Basic Section 40040:Aquatic life science-related / Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields / Aquatic bioproduction science Except Principal Investigator Basic Section 40040:Aquatic life science-related / Basic Section 40030:Aquatic bioproduction science-related
Keywords	Principal Investigator ゲノム編集 / 代理親魚技術 / トラフグ / クサフグ / 生殖細胞欠損 / 代理親魚 / 生殖幹細胞 / 連鎖解析 / 交雑 / 雑種 … More / 細胞移植 / トラフグ属 / 異種卵生産 / ｍRNA / 遺伝子レスキュー / mRNA / 不妊 / GnIH / メラトニン / 種苗生産 / CRISPR/Cas9 / 借り腹 / トランスフェクション / 発生工学 / 遺伝的多様性 / 生殖細胞 / 生殖細胞移植 / 栽培漁業 / 種苗放流 / 遺伝資源 / 凍結保存 … More Except Principal Investigator 代理親魚技術 / メダカ / ゲノム編集 / 不妊化技術 / 受精阻害 / 育種 / 種苗生産 / クロマグロ / 生殖細胞移植 / コシナガ / クロマグロ養殖 / 代理親魚技法 / マグロ属 / コシナガ人工授精 / ゲノム編集技術 / クサフグ / トラフグ / 全雌生産 / 生殖腺刺激ホルモン受容体 / 不妊化 / 代理親魚法 Less

小型のコシナガ代理親魚にクロマグロの卵と精子を生産させる。
- Principal Investigator
  
  矢澤良輔
- Project Period (FY)
  2024 – 2026
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 40030:Aquatic bioproduction science-related
- Research Institution
  Tokyo University of Marine Science and Technology
Development of a novel sterilization technique for cultured fish by targeting fertilization.
- Principal Investigator
  
  吉浦康寿
- Project Period (FY)
  2024 – 2026
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 40040:Aquatic life science-related
- Research Institution
  Fukui Prefectural University
Exploring surrogate broodstock fish genomes enabling xenogenic egg production through artificial hybridizationPrincipal Investigator
- Principal Investigator
  
  吉川廣幸
- Project Period (FY)
  2024 – 2026
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 40040:Aquatic life science-related
- Research Institution
  Fisheries Research and Education Agency
Development of non-transgenic gene rescue technology to reproduce next generation of genetically sterile fishPrincipal Investigator
- Principal Investigator
  
  吉川廣幸
- Project Period (FY)
  2023 – 2024
- Research Category
  
  Grant-in-Aid for Challenging Research (Exploratory)
- Review Section
  
  Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields
- Research Institution
  Fisheries Research and Education Agency
Development of a rapid gamete production technique using germ cell transplantation into a gonad of germ cell-less fish.Principal Investigator
- Principal Investigator
  
  Yoshikawa Hiroyuki
- Project Period (FY)
  2021 – 2023
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 40040:Aquatic life science-related
- Research Institution
  Fisheries Research and Education Agency
Does the knockout of melatonin- and gonadotropin-inhibitory-hormone-related genes induce early first maturity?Principal Investigator
- Principal Investigator
  
  Yoshikawa Hiroyuki
- Project Period (FY)
  2021 – 2022
- Research Category
  
  Grant-in-Aid for Challenging Research (Exploratory)
- Review Section
  
  Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields
- Research Institution
  Fisheries Research and Education Agency
Generation of "infertile fish" to prevent genetic pollution and development of its mass production technology
- Principal Investigator
  
  Yoshiura Yasutoshi
- Project Period (FY)
  2019 – 2021
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 40040:Aquatic life science-related
- Research Institution
  Fisheries Research and Education Agency
Development of the techniques for production of genome editing gametes with in vitro genome editing of germ line stem cells and surrogate broodstock technologyPrincipal Investigator
- Principal Investigator
  
  Yoshikawa Hiroyuki
- Project Period (FY)
  2018 – 2020
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 40040:Aquatic life science-related
- Research Institution
  Fisheries Research and Education Agency
Development of a new methodology to produce seedlings for stock enhancement: Production of seedlings derived from cryopreserved germ cells of wild tiger pufferPrincipal Investigator
- Principal Investigator
  
  Yoshikawa Hiroyuki
- Project Period (FY)
  2014 – 2015
- Research Category
  
  Grant-in-Aid for Research Activity Start-up
- Research Field
  
  Aquatic bioproduction science
- Research Institution
  National Fisheries University

All 2025 2024 2023 2022 2021 2020 2019 2018 2015

All Journal Article Presentation Book

[Book] フグ食の科学2021
- Author(s)
  酒井治己,池原強,石田武志,石丸恵理子,片町太輔,河邉真也,酒井一,椎木友朗,園山貴之,高橋洋,滝川裕子,辰野竜平,中村誠,濱田英嗣,古川澄明,古川幸弘,宮崎泰幸,山野上祐介,山本義久,吉浦康寿,吉川廣幸,鷲尾圭司
- Total Pages
  200
- Publisher
  生物研究社
- Data Source
  KAKENHI-PROJECT-18K05836
[Journal Article] Efficient production of donor-derived tiger puffer gametes from grass puffer recipient with germ cell deficiency by CRISPR-Cas9 mediated knockout of dead end 12025
- Author(s)
  Yoshikawa Hiroyuki、Ino Yasuko、Kishimoto Kenta、Kinoshita Masato、Yoshiura Yasutoshi
- Journal Title
  
  Aquaculture
  
  Volume: 595 Pages: 741626-741626
- DOI
  10.1016/j.aquaculture.2024.741626
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23K18057, KAKENHI-PROJECT-24K01856
[Journal Article] Enrichment of spermatogonia by density gradient centrifugation for use as a donor of surrogate production of tiger puffer2022
- Author(s)
  Yoshikawa Hiroyuki、Ino Yasuko、Shigematsu Atsushi、Yoshiura Yasutoshi
- Journal Title
  
  Aquaculture Research
  
  Volume: 53 Issue: 11 Pages: 4032-4044
- DOI
  10.1111/are.15905
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21H02289, KAKENHI-PROJECT-21K19156
[Journal Article] Induction of germ cell-deficiency in grass puffer by dead end 1 gene knockdown for use as a recipient in surrogate production of tiger puffer2020
- Author(s)
  Yoshikawa Hiroyuki、Ino Yasuko、Kishimoto Kenta、Koyakumaru Hayato、Saito Taiju、Kinoshita Masato、Yoshiura Yasutoshi
- Journal Title
  
  Aquaculture
  
  Volume: 526 Pages: 735385-735385
- DOI
  10.1016/j.aquaculture.2020.735385
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19H03056, KAKENHI-PROJECT-18K05836
[Presentation] Surrogate broodstock technology in tiger puffer and its application for breeding program2024
- Author(s)
  Hiroyuki Yoshikawa
- Organizer
  Fish genomics and aquaculture-oriented biotechnology. Wuhan, China
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-24K01856
[Presentation] Surrogate broodstock technology in tiger puffer and its application for breeding program2024
- Author(s)
  Hiroyuki Yoshikawa
- Organizer
  Fish genomics and aquaculture-oriented biotechnology. Wuhan, China
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K18057
[Presentation] クサフグ不妊アリル標識系統のトラフグ代理親魚技術への利用2024
- Author(s)
  吉川廣幸・井野靖子・岸本謙太・木下政人・吉浦康寿
- Organizer
  令和6年度日本水産学会秋季大会, 京都
- Data Source
  KAKENHI-PROJECT-24K01856
[Presentation] クサフグ不妊アリル標識系統のトラフグ代理親魚技術への利用2024
- Author(s)
  吉川廣幸・井野靖子・岸本謙太・木下政人・吉浦康寿
- Organizer
  令和6年度日本水産学会秋季大会, 京都
- Data Source
  KAKENHI-PROJECT-23K18057
[Presentation] 蛍光タンパク質遺伝子挿入によるクサフグの不妊形質の可視化2023
- Author(s)
  吉川廣幸, 井野靖子, 岸本謙太, 木下政人, 吉浦康寿
- Organizer
  令和5年度日本水産学会秋季大会
- Data Source
  KAKENHI-PROJECT-21H02289
[Presentation] 蛍光タンパク質遺伝子挿入によるクサフグの不妊形質の可視化2023
- Author(s)
  吉川廣幸, 井野靖子, 岸本謙太, 木下政人, 吉浦康寿
- Organizer
  令和5年度日本水産学会秋季大会
- Data Source
  KAKENHI-PROJECT-23K18057
[Presentation] ヒガンフグの生殖特性評価と人工排卵誘導技術の開発2023
- Author(s)
  吉川廣幸, 井野靖子, 三留明葵, 奥村友紀恵
- Organizer
  令和5年度日本水産学会中国・四国支部例会
- Data Source
  KAKENHI-PROJECT-23K18057
[Presentation] ヒガンフグの生殖特性評価と人工排卵誘導技術の開発2023
- Author(s)
  吉川廣幸, 井野靖子, 三留明葵, 奥村友紀恵
- Organizer
  令和5年度日本水産学会中国・四国支部例会
- Data Source
  KAKENHI-PROJECT-21H02289
[Presentation] Studies on surrogate broodstock technology in tiger puffer and its application for breeding program.2022
- Author(s)
  Yoshikawa H
- Organizer
  The 27th Joint International Symposium between Pukyong National University and National Fisheries University
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-21H02289
[Presentation] 代理親魚技術によるトラフグ生産と遺伝資源の保存管理技術の開発2022
- Author(s)
  吉川廣幸
- Organizer
  第22回マリンバイオテクノロジー学会シンポジウム「トラフグ養殖研究の最前線～次世代のトラフグ生産を科学する～」
- Invited
- Data Source
  KAKENHI-PROJECT-21H02289
[Presentation] 遺伝子破壊による生殖細胞欠損クサフグ宿主の作出2022
- Author(s)
  吉川廣幸, 井野靖子, 岸本謙太, 木下政人, 吉浦康寿
- Organizer
  令和4年度日本水産学会秋季大会
- Data Source
  KAKENHI-PROJECT-21H02289
[Presentation] クサフグasmt の単離と遺伝子破壊技術の開発2022
- Author(s)
  吉川廣幸, 井野靖子
- Organizer
  令和4 年度日本水産学会中国・四国支部例会
- Data Source
  KAKENHI-PROJECT-21K19156
[Presentation] 代理親魚技術によるトラフグ生産と遺伝資源の保存管理技術の開発2022
- Author(s)
  吉川廣幸
- Organizer
  第22回マリンバイオテクノロジー学会シンポジウム「トラフグ養殖研究の最前線～次世代のトラフグ生産を科学する～」
- Invited
- Data Source
  KAKENHI-PROJECT-21K19156
[Presentation] 遺伝子破壊による生殖細胞欠損クサフグ宿主の作出2022
- Author(s)
  吉川廣幸, 井野靖子, 岸本謙太, 木下政人, 吉浦康寿
- Organizer
  令和4年度日本水産学会秋季大会
- Data Source
  KAKENHI-PROJECT-21K19156
[Presentation] Studies on surrogate broodstock technology in tiger puffer and its application for breeding program.2022
- Author(s)
  Yoshikawa H
- Organizer
  The 27th Joint International Symposium between Pukyong National University and National Fisheries University
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-21K19156
[Presentation] 密度勾配遠心によるトラフグ精原細胞の濃縮と代理親技術への利用2021
- Author(s)
  吉川廣幸 , 井野靖子 , 吉浦康寿
- Organizer
  令和3年度日本水産学会秋季大会
- Data Source
  KAKENHI-PROJECT-21K19156
[Presentation] 密度勾配遠心によるトラフグ精原細胞の濃縮と代理親技術への利用2021
- Author(s)
  吉川廣幸, 井野靖子, 吉浦康寿
- Organizer
  令和3年度日本水産学会秋季大会
- Data Source
  KAKENHI-PROJECT-19H03056
[Presentation] 密度勾配遠心によるトラフグ精原細胞の濃縮と代理親技術への利用2021
- Author(s)
  吉川廣幸 , 井野靖子 , 吉浦康寿
- Organizer
  令和3年度日本水産学会秋季大会
- Data Source
  KAKENHI-PROJECT-21H02289
[Presentation] エレクトロポレーションによるトラフグ精巣細胞のゲノム編集技術の検討2019
- Author(s)
  吉川廣幸, 井野靖子, 岸本謙太, 木下政人, 吉浦康寿
- Organizer
  令和元年度日本水産学会秋季大会
- Data Source
  KAKENHI-PROJECT-18K05836
[Presentation] 代理親魚技術を用いた遺伝的不妊化魚の大量生産技術の開発2019
- Author(s)
  吉浦康寿、木下政人、吉川廣幸
- Organizer
  令和元年日本水産学会秋季大会ミニシンポジウム
- Invited
- Data Source
  KAKENHI-PROJECT-19H03056
[Presentation] クサフグdead endノックダウン宿主を利用したトラフグ配偶子生産2019
- Author(s)
  吉川廣幸, 井野靖子, 吉浦康寿
- Organizer
  令和元年度日本水産学会中国・四国支部例会
- Data Source
  KAKENHI-PROJECT-18K05836
[Presentation] 遺伝子ノックダウンによる生殖細胞欠損クサフグ宿主の作出2018
- Author(s)
  吉川廣幸, 井野靖子, 木下春哉, 小役丸隼人, 斎藤大樹, 岸本謙太, 木下政人, 吉浦康寿
- Organizer
  平成30年度日本水産学会秋季大会
- Data Source
  KAKENHI-PROJECT-18K05836
[Presentation] トラフグ属魚類精巣の凍結保存技術の開発2015
- Author(s)
  吉川廣幸, 井野靖子, 小松武史, 片山貴士, 吉浦康寿
- Organizer
  平成27年度日本水産学会秋季大会
- Place of Presentation
  仙台
- Year and Date
  2015-09-24
- Data Source
  KAKENHI-PROJECT-26892033

1. Yoshiura Yasutoshi (90372052)

# of Collaborated Projects: 3 results

# of Collaborated Products: 0 results
2. 木下政人 (60263125)

# of Collaborated Projects: 2 results

# of Collaborated Products: 4 results
3. 矢澤良輔 (70625863)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
4. 森田哲朗 (10833684)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
5. 吉崎悟朗 (70281003)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
6. 川村亘 (70973858)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
7. 亀井保博 (70372563)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
8. 高橋洋 (90399650)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
9. 吉浦康壽

# of Collaborated Projects: 0 results

# of Collaborated Products: 7 results

Yoshikawa Hiroyuki 吉川 廣幸

Research Projects

Research Products

Co-Researchers

小型のコシナガ代理親魚にクロマグロの卵と精子を生産させる。

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Development of a novel sterilization technique for cultured fish by targeting fertilization.

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Exploring surrogate broodstock fish genomes enabling xenogenic egg production through artificial hybridizationPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Development of non-transgenic gene rescue technology to reproduce next generation of genetically sterile fishPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Development of a rapid gamete production technique using germ cell transplantation into a gonad of germ cell-less fish.Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Does the knockout of melatonin- and gonadotropin-inhibitory-hormone-related genes induce early first maturity?Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Generation of "infertile fish" to prevent genetic pollution and development of its mass production technology

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Development of the techniques for production of genome editing gametes with in vitro genome editing of germ line stem cells and surrogate broodstock technologyPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Development of a new methodology to produce seedlings for stock enhancement: Production of seedlings derived from cryopreserved germ cells of wild tiger pufferPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

[Book] フグ食の科学2021

Author(s)

Total Pages

Publisher

Data Source

[Journal Article] Efficient production of donor-derived tiger puffer gametes from grass puffer recipient with germ cell deficiency by CRISPR-Cas9 mediated knockout of dead end 12025

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Enrichment of spermatogonia by density gradient centrifugation for use as a donor of surrogate production of tiger puffer2022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Induction of germ cell-deficiency in grass puffer by dead end 1 gene knockdown for use as a recipient in surrogate production of tiger puffer2020

Author(s)

Journal Title

DOI

Data Source

[Presentation] Surrogate broodstock technology in tiger puffer and its application for breeding program2024

Author(s)

Yoshikawa Hiroyuki 吉川廣幸