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Doukyu Noriyuki  道久 則之

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道久 則之  ドウキユウ ノリユキ

DOUKYU Noriyuki  道久 則之

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Researcher Number 60302957
Other IDs
Affiliation (Current) 2025: 東洋大学, 生命科学部, 教授
Affiliation (based on the past Project Information) *help 2024: 東洋大学, 生命科学部, 教授
2017 – 2019: 東洋大学, 生命科学部, 教授
2010 – 2014: 東洋大学, 生命科学部, 教授
2009: Toyo University, 生命科学部, 准教授
2005 – 2006: 東洋大学, 生命科学部, 助教授
2000 – 2002: 東京工業大学, 大学院・生命理工学研究科, 助手
Review Section/Research Field
Principal Investigator
Applied microbiology / Basic Section 38020:Applied microbiology-related / 応用微生物学・応用生物化学
Keywords
Principal Investigator
大腸菌 / 有機溶媒耐性 / 有機溶媒 / Chromobacterium / 臨床検査 / コレステロールオキシダーゼ / コレステロール / 有機化合物添加 / 有用物質生産 / 応用微生物 … More / キノン / AcrAB-TolC efflux pump / organic solvent tolerant / organic solvent / Escherichia coli / soxS / rob / marA / sox-marレギュロン遺伝子 / sox-marレギュロン / AcrAB-TolC排出ポンプ / グラム陰性細菌 / 溶媒排出ポンプ / AcrAB / 有機溶媒耐性生体触媒 / 有機溶媒耐性大腸菌 / 有機溶媒耐性微生物 Less
  • Research Projects

    (6 results)
  • Research Products

    (42 results)
  • Co-Researchers

    (2 People)
  •  有機化合物添加による大腸菌の高度有機溶媒耐性化と有用物質生産への応用Principal Investigator

    • Principal Investigator
      道久 則之
    • Project Period (FY)
      2024 – 2026
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Review Section
      Basic Section 38020:Applied microbiology-related
    • Research Institution
      Toyo University
  •  Construction of organic solvent-tolerant Escherichia coli based on quinone biosynthesisPrincipal Investigator

    • Principal Investigator
      Doukyu Noriyuki
    • Project Period (FY)
      2017 – 2019
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      Applied microbiology
    • Research Institution
      Toyo University
  •  Analysis of catalytic mechanism and activation of a highly stable cholesterol oxidasePrincipal Investigator

    • Principal Investigator
      DOUKYU Noriyuki
    • Project Period (FY)
      2012 – 2014
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      Applied microbiology
    • Research Institution
      Toyo University
  •  Analysis of a highly stable cholesterol oxidase from a gram-negative bacteriumPrincipal Investigator

    • Principal Investigator
      DOUKYU Noriyuki
    • Project Period (FY)
      2009 – 2011
    • Research Category
      Grant-in-Aid for Young Scientists (B)
    • Research Field
      Applied microbiology
    • Research Institution
      Toyo University
  •  有機溶媒耐性大腸菌を用いた有機溶媒耐性生体触媒の開発Principal Investigator

    • Principal Investigator
      道久 則之
    • Project Period (FY)
      2005 – 2006
    • Research Category
      Grant-in-Aid for Young Scientists (B)
    • Research Field
      Applied microbiology
    • Research Institution
      Toyo University
  •  Improvement of organic solvent tolerance level by directed evolution of AcrAB-TolC efflux pump in Escherichia coliPrincipal Investigator

    • Principal Investigator
      DOUKYU Noriyuki, 青野 力三
    • Project Period (FY)
      2000 – 2002
    • Research Category
      Grant-in-Aid for Scientific Research (B)
    • Research Field
      応用微生物学・応用生物化学
    • Research Institution
      Tokyo Institute of Technology

All 2019 2018 2017 2015 2014 2013 2012 2011 2010 2009 Other

All Journal Article Presentation Book

  • [Book] 極限環境生命, - 生命の起源を考え,その多様性に学ぶ -2014

    • Author(s)
      伊藤 政博, 鳴海 一成, 佐藤 孝子, 中村 聡, 東端 啓貴, 國技 武和, 為我井 秀行, 道久 則之, 伊藤 隆
    • Total Pages
      232
    • Publisher
      コロナ社
    • Data Source
      KAKENHI-PROJECT-24580127
  • [Journal Article] Cholesterol oxidase with high catalytic activity from Pseudomonas aeruginosa: Screening, molecular genetic analysis, expression and characterization.2015

    • Author(s)
      Noriyuki Doukyu, Shyou Nihei
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 未定 Issue: 1 Pages: 24-30

    • DOI

      10.1016/j.jbiosc.2014.12.003

    • NAID

      110009973280

    • Peer Reviewed / Acknowledgement Compliant
    • Data Source
      KAKENHI-PROJECT-24580127
  • [Journal Article] Novel cholesterol oxidases which were found in the studies of applications of organic solvent-tolerant bacteria2012

    • Author(s)
      Noriyuki Doukyu
    • Journal Title

      J.Jpn.Soc.Extremophiles

      Volume: 10 Pages: 13-16

    • NAID

      40019582650

    • Data Source
      KAKENHI-PROJECT-21780082
  • [Journal Article] Novel cholesterol oxidases which were found in the studies of applications of organic solvent-tolerant bacteria2011

    • Author(s)
      N. Doukyu
    • Journal Title

      J. Jpn. Soc. Extremophiles

      Volume: 10 Pages: 13-16

    • NAID

      40019582650

    • Data Source
      KAKENHI-PROJECT-21780082
  • [Journal Article] Structural characterization of the organic solvent-stable cholesterol oxidase from Chromobacterium sp2010

    • Author(s)
      M. Sagermann, A. Ohtaki, K. Newton, N. Doukyu
    • Journal Title

      J. Struct. Biol

      Volume: 170 Pages: 32-40

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Journal Article] Organic solvent-tolerant enzymes.2010

    • Author(s)
      Doukyu, N., Ogino, H.
    • Journal Title

      Biochem.Eng.J.

      Volume: 48 Pages: 270-282

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Journal Article] Structural characterization of the organic solvent-stable cholesterol oxidase from Chromobacterium sp. DS-1.2010

    • Author(s)
      Sagermann, M., Ohtaki, A., Newton, K., Doukyu, N.
    • Journal Title

      J.Struct.Biol. 170

      Pages: 32-40

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Journal Article] Structural characterization of the organic solvent-stable cholesterol oxidase from Chromobacterium sn.DS-1.2010

    • Author(s)
      Sagermann, M., Ohtaki, A., Newton, K., Doukyu, N.
    • Journal Title

      J.Struct.Biol.

      Volume: 170 Pages: 32-40

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Journal Article] Organic solvent-tolerant enzymes.2010

    • Author(s)
      Doukyu, N., Ogino, H.
    • Journal Title

      Biochem.Eng.J. 48

      Pages: 270-282

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Journal Article] Organic solvent-tolerant enzymes2010

    • Author(s)
      N. Doukyu and H. Ogino
    • Journal Title

      Biochem. Eng. J

      Volume: 48 Pages: 270-282

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Journal Article] Charactedstics and biotechnological applications of microbial cholesterol oxidases.2009

    • Author(s)
      Doukyu, N.
    • Journal Title

      Appl.Microbiol.Biotechnol. 83

      Pages: 825-837

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Journal Article] Characteristics and biotechnological applications of microbial cholesterol oxidases2009

    • Author(s)
      N. Doukyu
    • Journal Title

      Appl. Microbiol. Biotechnol

      Volume: 83 Pages: 825-837

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Presentation] Involvement of nano efflux pump in vanillin-tolerance in Escherichia coli2019

    • Author(s)
      Noriyuki Doukyu
    • Organizer
      17th International Symposium on Bioscience and Nanotechnology
    • Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-17K07731
  • [Presentation] Organic solvent tolerance of Escherichia coli mutant that is tolerant to a mixture of p-xylene and toluene2018

    • Author(s)
      M. Miki and N. Doukyu
    • Organizer
      The 12th International Congress on Extremophiles
    • Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-17K07731
  • [Presentation] キノン合成中間体による大腸菌の有機溶媒耐性化機構2018

    • Author(s)
      藤澤 秀之、道久則之
    • Organizer
      極限環境生物学会
    • Data Source
      KAKENHI-PROJECT-17K07731
  • [Presentation] Involvement of a metabolite of menaquinone biosynthesis in organic solvent tolerance in Escherichia coli.2018

    • Author(s)
      H. Fujisawa and N. Doukyu
    • Organizer
      The 12th International Congress on Extremophiles
    • Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-17K07731
  • [Presentation] 高度な有機溶媒耐性を有する大腸菌変異株の解析2018

    • Author(s)
      三木 護、道久則之
    • Organizer
      極限環境生物学会
    • Data Source
      KAKENHI-PROJECT-17K07731
  • [Presentation] 高度に有機溶媒耐性化した大腸菌変異株の解析2017

    • Author(s)
      三木 護、道久則之
    • Organizer
      極限環境生物学会
    • Data Source
      KAKENHI-PROJECT-17K07731
  • [Presentation] メナキノン合成中間代謝物による大腸菌の有機溶媒耐性化2017

    • Author(s)
      藤澤 秀之、道久則之
    • Organizer
      極限環境生物学会
    • Data Source
      KAKENHI-PROJECT-17K07731
  • [Presentation] Rhodococcus erythropolis 由来コレステロールオキシダーゼの大腸菌による発現2013

    • Author(s)
      石川誠、道久則之
    • Organizer
      極限環境微生物学会
    • Place of Presentation
      明治大学 生田キャンパス(神奈川県)
    • Data Source
      KAKENHI-PROJECT-24580127
  • [Presentation] Pseudomonas aeruginosa 由来コレステロールオキシダーゼの解析2012

    • Author(s)
      道久則之、二瓶翔
    • Organizer
      極限環境微生物学会
    • Place of Presentation
      日本大学
    • Data Source
      KAKENHI-PROJECT-24580127
  • [Presentation] 組換え大腸菌によるPseudomonas aeruginosa由来コレステロールオキシダーゼの生産2011

    • Author(s)
      二瓶翔、道久則之
    • Organizer
      極限環境微生物学会
    • Place of Presentation
      長崎大学(長崎県)
    • Year and Date
      2011-11-27
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Presentation] Novel microbial cholesterol oxidases-useful enzymes for multiple applications2011

    • Author(s)
      N. Doukyu
    • Organizer
      9^<th> International Symposium on Bioscience and Nanotechnology
    • Place of Presentation
      Kawagoe, Japan
    • Year and Date
      2011-12-10
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Presentation] Chromobacterium. sp DS-1株由来コレステロールオキシダーゼの触媒部位の解析2011

    • Author(s)
      小野奈津美、道久則之
    • Organizer
      極限環境微生物学会2011年度年会
    • Place of Presentation
      長崎大学
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Presentation] Chromobacterium.sp DS-1株由来コレステロールオキシダーゼの触媒部位の解析2011

    • Author(s)
      小野奈津美、道久則之
    • Organizer
      極限環境微生物学会
    • Place of Presentation
      長崎大学(長崎県)
    • Year and Date
      2011-11-27
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Presentation] 組換え大腸菌によるPseudomonas aeruginosa由来コレステロールオキシダーゼの生産2011

    • Author(s)
      二瓶翔、道久則之
    • Organizer
      極限環境微生物学会2011年度年会
    • Place of Presentation
      長崎大学
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Presentation] Characterization of cholesterol oxidases from gram negative bacteria2011

    • Author(s)
      S. Nihei, N. Ono, N. Doukyu, M. Sagermann
    • Organizer
      9^<th> International Symposium on Bioscience and Nanotechnology
    • Place of Presentation
      Kawagoe, Japan
    • Year and Date
      2011-12-10
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Presentation] Novel cholesterol oxidases from bacteria-Useful enzymes for multiple applications2011

    • Author(s)
      N. Doukyu
    • Organizer
      BNERC. IIT Delhi Seminar, International Seminar for the 125^<th> Anniversary of Toyo University
    • Place of Presentation
      India, Delhi
    • Year and Date
      2011-08-02
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Presentation] Cholesterol oxidases from organic solvent-tolerant bacteria2010

    • Author(s)
      N. Doukyu
    • Organizer
      The 8th International Congress on Extremophiles
    • Place of Presentation
      Ponta Delgada. Portugal
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Presentation] 臨床検査に役立つ酵素~細菌のコレステロールオキシダーゼの研究~2010

    • Author(s)
      道久則之
    • Organizer
      東洋大学生命科学部シンポジウム
    • Place of Presentation
      東洋大学板倉校舎
    • Year and Date
      2010-11-26
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Presentation] Chromobacterium属細菌由来コレステロールオキシダーゼ遺伝子のクローニングと発現2010

    • Author(s)
      道久則之、柴田貫平
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京(東京大学)
    • Year and Date
      2010-03-29
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Presentation] コレステロール値を測定するための安定なコレステロールオキシダーゼ2010

    • Author(s)
      道久則之
    • Organizer
      大学連携新技術説明会
    • Place of Presentation
      JSTホール東京
    • Year and Date
      2010-10-29
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Presentation] 熱、有機溶媒、界面活性剤耐性のコレステロールオキシダーゼの解析2010

    • Author(s)
      道久則之
    • Organizer
      CPhI Japan, TLO/大学知的財産本部セミナー
    • Place of Presentation
      東京ビッグサイト
    • Year and Date
      2010-04-23
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Presentation] 有機溶媒耐性菌の応用研究から見出された新規なコレステロール酸化酵素に関する研究2010

    • Author(s)
      道久則之
    • Organizer
      極限環境微生物学会2010年度年会、極限環境生物学会・研究奨励賞講演
    • Place of Presentation
      京都大学宇治キャンパス
    • Year and Date
      2010-11-16
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Presentation] Chromobacterium属細菌由来コレステロールオキシダーゼ遺伝子のクローニングと発現2010

    • Author(s)
      道久則之、柴田貫平
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-29
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Presentation] Cholesterol oxidases from organic solvent-tolerant bacteria.2010

    • Author(s)
      道久則之
    • Organizer
      The 8th International Congress on Extremophiles
    • Place of Presentation
      Ponta Delgada, Portugal
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Presentation] 有機溶媒耐性菌の応用研究から見出された新規なコレステロール酸化酵素に関する研究2010

    • Author(s)
      道久則之
    • Organizer
      極限環境微生物学会 第11回年会
    • Place of Presentation
      京都(京都大学)
    • Year and Date
      2010-11-16
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Presentation] Analysis of highly stable cholesterol oxidases from gram negative bacteria2010

    • Author(s)
      N. Ono, S. Nihei, N. Doukyu, M. Sagermann
    • Organizer
      8^<th> International Symposium on Bioscience and Nanotechnology
    • Place of Presentation
      Tokyo
    • Year and Date
      2010-12-10
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Presentation] Chromobacterium属細菌由来コレステロールオキシダーゼ遺伝子のクローニング2009

    • Author(s)
      道久則之
    • Organizer
      極限環境微生物学会第10回年会
    • Place of Presentation
      明治大学
    • Year and Date
      2009-10-29
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Presentation] Cloning, sequence analysis and expression of a gene encoding Chromobacterium sp. DS-1 cholesterol oxidase2009

    • Author(s)
      N. Doukyu, K. Shibata, M. Sagermann
    • Organizer
      7^<th> International Symposium on Bioscience and Nanotechnology
    • Place of Presentation
      Toyo University
    • Year and Date
      2009-11-20
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Presentation] Chromobacterium属細菌由来コレステロールオキシダーゼ遺伝子のクローニング2009

    • Author(s)
      道久則之
    • Organizer
      極限環境微生物学会第10回年会
    • Place of Presentation
      東京(明治大学)
    • Year and Date
      2009-10-29
    • Data Source
      KAKENHI-PROJECT-21780082
  • [Presentation] Rhodococcus erythropolis 由来コレステロールオキシダーゼのコレステロール酸化物の解析

    • Author(s)
      石川誠、道久則之
    • Organizer
      第15回 極限環境微生物学会年会
    • Place of Presentation
      今帰仁村コミュニティーセンター(沖縄県国頭郡)
    • Year and Date
      2014-11-01 – 2014-11-03
    • Data Source
      KAKENHI-PROJECT-24580127
  • 1.  青野 力三 (30126643)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 2.  TSUKAGOSHI Norihiko
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results

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