Tsukiyama Tomoyuki 築山智之

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TSUKIYAMA Tomoyuki 築山智之

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Researcher Number	60612132
Other IDs	https://orcid.org/0000-0002-2657-7719
Affiliation (Current)	2025: 滋賀医科大学, 動物生命科学研究センター, 特任准教授
Affiliation (based on the past Project Information) *help	2019 – 2025: 滋賀医科大学, 動物生命科学研究センター, 特任准教授 2014 – 2018: 滋賀医科大学, 動物生命科学研究センター, 助教 2013: 独立行政法人理化学研究所, 発生・再生科学総合研究センター, 研究員
Review Section/Research Field	Principal Investigator Medium-sized Section 42:Veterinary medical science, animal science, and related fields / Basic Section 42030:Animal life science-related / General medical chemistry / Transformative Research Areas, Section (III) / Integrative animal science Except Principal Investigator Basic Section 53040:Nephrology-related / Medium-sized Section 56:Surgery related to the biological and sensory functions and related fields / Basic Section 42030:Animal life science-related / Broad Section I / Broad Section G / Biological Sciences / Basic Section 42040:Laboratory animal science-related
Keywords	Principal Investigator カニクイザル / ゲノム編集 / 遺伝子改変 / ノックイン / iPS細胞 / CRISPR/Cas9 / ES細胞 / 多能性幹細胞 / 霊長類 / ナイーブ化 … More / トランスジェニック動物 / piggyBac / トランスポゾン / TS細胞 / ラットiPS細胞 / INTPS細胞 / 新型多能性幹細胞 / 異種間キメラ … More Except Principal Investigator 霊長類 / トロホブラスト / 細胞ヒエラルキー / 幹細胞 / 妊娠高血圧症 / 胎盤 / 幹細胞ヒエラルキー / 空間トランスクリプトーム / 動物モデル / 栄養外胚葉 / 羊膜外胚葉 / 胚体外組織 / ヒトES細胞 / fMRI / 意思決定 / ドパミン / 可塑性 / 機能回復 / 遺伝子発現解析 / 精神疾患 / 依存症 / 脊髄損傷 / 急性腎障害 / シングルセル / 線維芽細胞 / エリスロポエチン / 老化関連T細胞 / シングルセル解析 / 亜集団 / 三次リンパ組織 / 細胞老化 / 慢性腎臓病 / リプログラミング / 種差 / トポロジー / ロバストネス / 自己組織化 / 哺乳類初期発生 / 発生生物学 / マウス / 減数分裂 / 試験管内再構成 / 発生 / ヒト / 進化 / 発現制御 / ゲノム / 再生医学 / 発生・分化 / ヒト発生生物学 / 試験管内誘導 / 卵母細胞 / エピゲノムリプログラミング / iPS細胞 / 生殖細胞 / ゲノム編集 / 霊長類モデル / 早期老化症候群 Less

モザイク性の解消による早期表現型解析のための遺伝子改変技術基盤の構築Principal Investigator
- Principal Investigator
  
  築山智之
- Project Period (FY)
  2025 – 2027
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 42030:Animal life science-related
- Research Institution
  Shiga University of Medical Science
能動的組換えによる革新的遺伝子ノックインPrincipal Investigator
- Principal Investigator
  
  築山智之
- Project Period (FY)
  2024 – 2025
- Research Category
  
  Grant-in-Aid for Challenging Research (Exploratory)
- Review Section
  
  Medium-sized Section 42:Veterinary medical science, animal science, and related fields
- Research Institution
  Shiga University of Medical Science
Elucidation of the stem cell hierarchy that constitutes the placenta and functional improvement of trophoblast stem cells
- Principal Investigator
  
  高島康弘
- Project Period (FY)
  2024 – 2028
- Research Category
  
  Grant-in-Aid for Scientific Research (A)
- Review Section
  
  Medium-sized Section 56:Surgery related to the biological and sensory functions and related fields
- Research Institution
  Kyoto University
Partial reprogramming of trophoblast stem cells to improve placental function
- Principal Investigator
  
  高島康弘
- Project Period (FY)
  2023 – 2024
- Research Category
  
  Grant-in-Aid for Challenging Research (Exploratory)
- Review Section
  
  Medium-sized Section 56:Surgery related to the biological and sensory functions and related fields
- Research Institution
  Kyoto University
Primate Nephrology: analyzing primate-specific repair mechanisms of renal injury
- Principal Investigator
  
  柳田素子
- Project Period (FY)
  2023 – 2025
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 53040:Nephrology-related
- Research Institution
  Kyoto University
Development of innovative knock-in technology by active gene transferPrincipal Investigator
- Principal Investigator
  
  TSUKIYAMA Tomoyuki
- Project Period (FY)
  2022 – 2023
- Research Category
  
  Grant-in-Aid for Challenging Research (Exploratory)
- Review Section
  
  Medium-sized Section 42:Veterinary medical science, animal science, and related fields
- Research Institution
  Shiga University of Medical Science
Elucidating the pathogenesis of and development of therapeutic strategies against the neuropsychiatric disorders in the primate models by utilizing the gene introduction and manipulation technologies
- Principal Investigator
  
  伊佐正
- Project Period (FY)
  2022 – 2026
- Research Category
  
  Grant-in-Aid for Scientific Research (S)
- Review Section
  
  Broad Section I
- Research Institution
  Kyoto University
遺伝子改変動物作出におけるモザイク性の解消Principal Investigator
- Principal Investigator
  
  築山智之
- Project Period (FY)
  2022 – 2024
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 42030:Animal life science-related
- Research Institution
  Shiga University of Medical Science
幹細胞を用いた羊膜前駆細胞の発生機序と細胞特性解析
- Principal Investigator
  
  大串雅俊
- Project Period (FY)
  2022 – 2024
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 42030:Animal life science-related
- Research Institution
  Kyoto University
Spatio-temporal variabilities and robustness in pre-implantation mammalian development
- Principal Investigator
  
  柊卓志
- Project Period (FY)
  2021 – 2025
- Research Category
  
  Grant-in-Aid for Scientific Research (S)
- Review Section
  
  Broad Section G
- Research Institution
  Kyoto University
Development of innovative knock-in technology using endogenous homologous recombination mechanismPrincipal Investigator
- Principal Investigator
  
  TSUKIYAMA Tomoyuki
- Project Period (FY)
  2020 – 2021
- Research Category
  
  Grant-in-Aid for Challenging Research (Exploratory)
- Review Section
  
  Medium-sized Section 42:Veterinary medical science, animal science, and related fields
- Research Institution
  Shiga University of Medical Science
Establishment of a technology platform for genetic modification of fertilized eggs that enables analysis from the F0 generationPrincipal Investigator
- Principal Investigator
  
  Tsukiyama Tomoyuki
- Project Period (FY)
  2020 – 2022
- Research Category
  
  Grant-in-Aid for Transformative Research Areas (B)
- Review Section
  
  Transformative Research Areas, Section (III)
- Research Institution
  Shiga University of Medical Science
Cellular senescence of renal "subpopulations" influences renal aging and injury responsiveness
- Principal Investigator
  
  Yanagita Motoko
- Project Period (FY)
  2020 – 2022
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 53040:Nephrology-related
- Research Institution
  Kyoto University
Development of diagnosis and therapy for aging using a nonhuman primate model of premature aging
- Principal Investigator
  
  Itoh Yasushi
- Project Period (FY)
  2019 – 2021
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 42040:Laboratory animal science-related
- Research Institution
  Shiga University of Medical Science
Establishment of high-quality novel ES / iPS cells that precisely reflect the in vivo statePrincipal Investigator
- Principal Investigator
  
  TSUKIYAMA Tomoyuki
- Project Period (FY)
  2017 – 2019
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Integrative animal science
- Research Institution
  Shiga University of Medical Science
Mechanism and Reconstitution In Vitro of Human Germ Cell Development
- Principal Investigator
  
  Saitou Mitinori
- Project Period (FY)
  2017 – 2021
- Research Category
  
  Grant-in-Aid for Specially Promoted Research
- Review Section
  
  Biological Sciences
- Research Institution
  Kyoto University
Establishment of novel pluripotent stem cells using genetically modified cynomolgus ES and iPS cells.Principal Investigator
- Principal Investigator
  
  TSUKIYAMA Tomoyuki
- Project Period (FY)
  2015 – 2016
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  General medical chemistry
- Research Institution
  Shiga University of Medical Science
Establishment of novel pluripotent stem cells in mammals by iPS technology.Principal Investigator
- Principal Investigator
  
  TSUKIYAMA Tomoyuki
- Project Period (FY)
  2013 – 2014
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  General medical chemistry
- Research Institution
  Shiga University of Medical Science
  Institute of Physical and Chemical Research

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All Journal Article Presentation Book

[Book] 実験医学別冊マウス表現型解析スタンダード2016
- Author(s)
  築山智之、依馬正次
- Total Pages
  347
- Publisher
  羊土社
- Data Source
  KAKENHI-PROJECT-15K21080
[Journal Article] Null mutation of exocyst complex component 3-like does not affect vascular development in mice2024
- Author(s)
  Takashima Satsuki、Okamura Eiichi、Ichiyama Yusuke、Nishi Kiyoto、Shimizu Akio、Watanabe Chisato、Muto Masanaga、Matsumoto Shoma、Tsukiyama-Fujii Setsuko、Tsukiyama Tomoyuki、Ogita Hisakazu、Nishi Eiichiro、Ohji Masahito、Sugiyama Fumihiro、Takahashi Satoru、Mizuno Seiya、Mizutani Ken-ichi、Ema Masatsugu
- Journal Title
  
  Exp. Anim.
  
  Volume: 73 Issue: 1 Pages: 93-100
- DOI
  10.1538/expanim.23-0105
- ISSN
  0007-5124, 1341-1357, 1881-7122
- Language
  English
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-23KJ1146, KAKENHI-PROJECT-23K07966, KAKENHI-PROJECT-23K08732, KAKENHI-PROJECT-21K06854, KAKENHI-PROJECT-22K19246, KAKENHI-PROJECT-22K19428, KAKENHI-PROJECT-22H04922, KAKENHI-PROJECT-23K23794
[Journal Article] Temporal variability and cell mechanics control robustness in mammalian embryogenesis2023
- Author(s)
  Fabreges, D., Corominas Murtra, B., Moghe, P., Kickuth, A., Ichikawa, T., Iwatani, C., Tsukiyama, T., Daniel, N., Gering, J., Stokkermans, A., Wolny, A., Kreshuk, A., Duranthon, V., Uhlmann, V., Hannezo, E. and Hiiragi, T
- Journal Title
  
  bioRxiv
  
  Volume: NA
- DOI
  10.1101/2023.01.24.525420
- Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-21H05038
[Journal Article] Induction of fetal meiotic oocytes from embryonic stem cells in cynomolgus monkeys2023
- Author(s)
  Gyobu‐Motani Sayuri、Yabuta Yukihiro、Mizuta Ken、Katou Yoshitaka、Okamoto Ikuhiro、Kawasaki Masanori、Kitamura Ayaka、Tsukiyama Tomoyuki、Iwatani Chizuru、Tsuchiya Hideaki、Tsujimura Taro、Yamamoto Takuya、Nakamura Tomonori、Saitou Mitinori
- Journal Title
  
  The EMBO Journal
  
  Volume: - Issue: 9
- DOI
  10.15252/embj.2022112962
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-22K19246, KAKENHI-PROJECT-22K15380, KAKENHI-ORGANIZER-20H05760, KAKENHI-PLANNED-20H05763, KAKENHI-PROJECT-23K23794, KAKENHI-PROJECT-23K23804, KAKENHI-PROJECT-21K20740, KAKENHI-PROJECT-17H06098
[Journal Article] Generation of a familial hypercholesterolemia model in non-human primate2023
- Author(s)
  Sato Akira、Tsukiyama Tomoyuki、Komeno Masahiro、Iwatani Chizuru、Tsuchiya Hideaki、Kawamoto Ikuo、Murase Mitsuru、Nakagawa Takahiro、Itagaki Iori、Seita Yasunari、Matsumoto Shoma、Nakaya Masataka、Shimizu Akio、Yamada Atsushi、Ema Masatsugu、Ogita Hisakazu
- Journal Title
  
  Scientific Reports
  
  Volume: 13 Issue: 1 Pages: 15649-15649
- DOI
  10.1038/s41598-023-42763-1
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-23K08732, KAKENHI-PROJECT-21K06854, KAKENHI-PROJECT-21K09419, KAKENHI-PROJECT-22K19246, KAKENHI-PROJECT-22H04922, KAKENHI-PROJECT-23K23794
[Journal Article] <i>Ex vivo</i> reconstitution of fetal oocyte development in humans and cynomolgus monkeys2022
- Author(s)
  Mizuta Ken、Katou Yoshitaka、Nakakita Baku、Kishine Aoi、Nosaka Yoshiaki、Saito Saki、Iwatani Chizuru、Tsuchiya Hideaki、Kawamoto Ikuo、Nakaya Masataka、Tsukiyama Tomoyuki、Nagano Masahiro、Kojima Yoji、Nakamura Tomonori、Yabuta Yukihiro、Horie Akihito、Mandai Masaki、Ohta Hiroshi、Saitou Mitinori
- Journal Title
  
  The EMBO Journal
  
  Volume: 41 Issue: 18
- DOI
  10.15252/embj.2022110815
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-22K06066, KAKENHI-PROJECT-22K19246, KAKENHI-ORGANIZER-20H05760, KAKENHI-PLANNED-20H05763, KAKENHI-PROJECT-19H03618, KAKENHI-PROJECT-20K21500, KAKENHI-PROJECT-19K16023, KAKENHI-PROJECT-23K23794, KAKENHI-PROJECT-21K20740
[Journal Article] Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice2022
- Author(s)
  Watanabe C., Shibuya H., Ichiyama Y., Okamura E., Tsukiyama-Fujii S., Tsukiyama T., Matsushita J., Azami T., Kubota Y., Ohji M., Sugiyama F., Takahashi S., Mizuno S., Tamura M., Mizutani K. & Ema M.
- Journal Title
  
  Life
  
  Volume: 12 Issue: 11 Pages: 1730-1730
- DOI
  10.3390/life12111730
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-22K06802, KAKENHI-PROJECT-22K19246, KAKENHI-ORGANIZER-20H05760, KAKENHI-PLANNED-20H05763, KAKENHI-PROJECT-21J15321, KAKENHI-PROJECT-23K23794
[Journal Article] Conformational change of RNA-helicase DHX30 by ALS/FTD-linked FUS induces mitochondrial dysfunction and cytosolic aggregates2022
- Author(s)
  Hikiami R, Morimura T, Ayaki T, Tsukiyama T, Morimura N, Kusui M, Wada H, Minamiyama S, Shodai A, Asada-Utsugi M, Muramatsu SI, Ueki T, Takahashi R, Urushitani M.
- Journal Title
  
  Scxeintific Reports
  
  Volume: 12 Issue: 1 Pages: 16030-16046
- DOI
  10.1038/s41598-022-20405-2
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-20K06391, KAKENHI-PROJECT-22K06429, KAKENHI-PROJECT-22K19246, KAKENHI-ORGANIZER-20H05760, KAKENHI-PLANNED-20H05763, KAKENHI-PROJECT-19K16277, KAKENHI-PROJECT-23K23794, KAKENHI-PROJECT-23K24211
[Journal Article] Non-human primates as a model for human development2021
- Author(s)
  Nakamura Tomonori、Fujiwara Kohei、Saitou Mitinori、Tsukiyama Tomoyuki
- Journal Title
  
  Stem Cell Reports
  
  Volume: 16 Issue: 5 Pages: 1093-1103
- DOI
  10.1016/j.stemcr.2021.03.021
- NAID
  120007037861
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-20K21370, KAKENHI-PLANNED-20H05763
[Journal Article] Chromosomal-scale de novo genome assemblies of Cynomolgus Macaque and Common Marmoset2021
- Author(s)
  Jayakumar Vasanthan、Nishimura Osamu、Kadota Mitsutaka、Hirose Naoki、Sano Hiromi、Murakawa Yasuhiro、Yamamoto Yumiko、Nakaya Masataka、Tsukiyama Tomoyuki、Seita Yasunari、Nakamura Shinichiro、Kawai Jun、Sasaki Erika、Ema Masatsugu、Kuraku Shigehiro、Kawaji Hideya、Sakakibara Yasubumi
- Journal Title
  
  Scientific Data
  
  Volume: 8 Issue: 1 Pages: 59-59
- DOI
  10.1038/s41597-021-00935-6
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-20K21370, KAKENHI-PROJECT-20K21407, KAKENHI-PLANNED-17H06410, KAKENHI-PLANNED-20H05763, KAKENHI-PROJECT-18H04127, KAKENHI-PROJECT-19K16023, KAKENHI-PROJECT-20KK0186
[Journal Article] GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program2021
- Author(s)
  Kojima Yoji、Yamashiro Chika、Murase Yusuke、Yabuta Yukihiro、Okamoto Ikuhiro、Iwatani Chizuru、Tsuchiya Hideaki、Nakaya Masataka、Tsukiyama Tomoyuki、Nakamura Tomonori、Yamamoto Takuya、Saitou Mitinori
- Journal Title
  
  Life Science Alliance
  
  Volume: 4 Issue: 5 Pages: 202000974-202000974
- DOI
  10.26508/lsa.202000974
- NAID
  120006979739
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-20K21370, KAKENHI-PROJECT-19K16023, KAKENHI-ORGANIZER-20H05760, KAKENHI-PLANNED-20H05763, KAKENHI-PROJECT-18K06030, KAKENHI-PROJECT-17H06098, KAKENHI-PLANNED-20H05761, KAKENHI-PROJECT-18K14714, KAKENHI-PROJECT-20K06654
[Journal Article] Induction of the germ cell fate from pluripotent stem cells in cynomolgus monkeys.2020
- Author(s)
  Sakai Y, Nakamura T, Okamoto I, Gyobu-Motani S, Ohta H, Yabuta Y, Tsukiyama T, Iwatani C, Tsuchiya H, Ema M, Morizane A, Takahashi J, Yamamoto T, Saitou M.
- Journal Title
  
  Biol Reprod
  
  Volume: 102(3) Issue: 3 Pages: 620-638
- DOI
  10.1093/biolre/ioz205
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18K06030, KAKENHI-PROJECT-19K16013, KAKENHI-PROJECT-19H03618, KAKENHI-PROJECT-17K08135, KAKENHI-PROJECT-18K14714, KAKENHI-PROJECT-17H06098
[Journal Article] Generation of transgenic cynomolgus monkeys overexpressing the gene for amyloid-β precursor protein2020
- Author(s)
  Seita Y, Morimura T, Watanabe N, Iwatani C, Tsuchiya H, Nakamura S, Suzuki T, Yanagisawa D, Tsukiyama T, Nakaya M, Okamura E, Muto M, Ema M, Nishimura M, Tooyama I.
- Journal Title
  
  Journal of Alzheimer's Disease
  
  Volume: - Issue: 1 Pages: 45-60
- DOI
  10.3233/jad-191081
- NAID
  120006859791
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-19K21585, KAKENHI-PROJECT-19H03546, KAKENHI-PROJECT-17K01355, KAKENHI-PROJECT-17K08077, KAKENHI-PROJECT-17K08135, KAKENHI-PROJECT-17K17812, KAKENHI-PROJECT-20K21370, KAKENHI-PROJECT-19K16023, KAKENHI-PROJECT-20K16491, KAKENHI-PLANNED-20H05763, KAKENHI-PROJECT-17H03560, KAKENHI-PROJECT-18H02566
[Journal Article] Generation of an OCT3/4 reporter cynomolgus monkey ES cell line using CRISPR/Cas9.2019
- Author(s)
  Kobayashi K, Tsukiyama T, Nakaya M, Kageyama S, Tomita K, Murai R, Yoshida T, Narita M, Kawauchi A, Ema M.
- Journal Title
  
  Stem cell research
  
  Volume: Epub Pages: 101439-101439
- DOI
  10.1016/j.scr.2019.101439
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-16K20131, KAKENHI-PROJECT-17H06766, KAKENHI-PROJECT-18K09192, KAKENHI-PROJECT-17K08135
[Journal Article] Generating Vegfr3 reporter transgenic mouse expressing membrane-tagged Venus for visualization of VEGFR3 expression in vascular and lymphatic endothelial cells2019
- Author(s)
  Watanabe C., Matsushita J., Azami T., Tsukiyama-Fujii S., Tsukiyama T., Mizuno S., Takahashi S., and Ema M.
- Journal Title
  
  PLOS ONE
  
  Volume: 14 Issue: 1 Pages: e0210060-e0210060
- DOI
  10.1371/journal.pone.0210060
- NAID
  120007128325
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-17K08135, KAKENHI-PROJECT-18H02363
[Journal Article] Monkeys mutant for PKD1 recapitulate human autosomal dominant polycystic kidney disease2019
- Author(s)
  Tsukiyama T, Kobayashi K, Nakaya M, Iwatani C, Seita Y, Tsuchiya H, Matsushita J, Kitajima K, Kawamoto I, Nakagawa T, Fukuda K, Iwakiri T, Izumi H, Itagaki I, Kume S, Maegawa H, Nishinakamura R, Nishio, Nakamura S, Kawauchi A, Ema M
- Journal Title
  
  Nature Communications
  
  Volume: 10 (1) Issue: 1 Pages: 5517-5517
- DOI
  10.1038/s41467-019-13398-6
- NAID
  120006773159
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-19K22363, KAKENHI-PROJECT-19K16023, KAKENHI-PROJECT-17K08135, KAKENHI-PROJECT-17K09679, KAKENHI-PROJECT-16K20131
[Journal Article] Comprehensive evaluation of ubiquitous promoters suitable for the generation of transgenic cynomolgus monkeys†2019
- Author(s)
  Seita Yasunari、Tsukiyama Tomoyuki、Azami Takuya、Kobayashi Kenichi、Iwatani Chizuru、Tsuchiya Hideaki、Nakaya Masataka、Tanabe Hideyuki、Hitoshi Seiji、Miyoshi Hiroyuki、Nakamura Shinichiro、Kawauchi Akihiro、Ema Masatsugu
- Journal Title
  
  Biology of Reproduction
  
  Volume: 印刷中 Issue: 6 Pages: 1440-1452
- DOI
  10.1093/biolre/ioz040
- NAID
  120006594188
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17H06766, KAKENHI-PROJECT-16K14578, KAKENHI-PROJECT-17K14977, KAKENHI-PROJECT-17K08135, KAKENHI-PROJECT-16K20131, KAKENHI-PROJECT-16H04671
[Journal Article] Flexible adaptation of male germ cells from female iPSCs of endangered Tokudaia osimensis2017
- Author(s)
  Arata Honda, Narantsog Choijookhuu, Haruna Izu, Yoshihiro Kawano, Mizuho Inokuchi, Kimiko Honsho, Ah-Reum Lee, Hiroki Nabekura, Hiroshi Ohta, Tomoyuki Tsukiyama, Yasuhide Ohinata, Asato Kuroiwa, Yoshitaka Hishikawa, Mitinori Saitou, Takamichi Jogahara, Chihiro Koshimoto
- Journal Title
  
  Science Advances
  
  Volume: - Issue: 5
- DOI
  10.1126/sciadv.1602179
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-25712030, KAKENHI-PROJECT-16K08471, KAKENHI-PROJECT-17K08135, KAKENHI-PROJECT-26430092, KAKENHI-PROJECT-15H04286, KAKENHI-PROJECT-15K14887, KAKENHI-PROJECT-17H06098
[Journal Article] Flexible adaptation of male germ cells from female iPS cells of endangered Tokudaia osimensis2017
- Author(s)
  Honda A., Choijookhuu N., Izu H., Kawano Y., Inokuchi M., Honsho K., Lee A., Nabekura H., Ohta H., Tsukiyama T., Ohinata Y., Kuroiwa A., Hishikawa Y., Saitou M., Jogahara T., Koshimoto C.
- Journal Title
  
  Science Advances
  
  Volume: 印刷中
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-15K21080
[Journal Article] Derivation of Induced Trophoblast Cell Lines in Cattle by Doxycycline-Inducible piggyBac Vectors2016
- Author(s)
  Kawaguchi T, Cho D, Hayashi M, Tsukiyama T, Kimura K, Matsuyama S, Minami N, Yamada M and Imai H
- Journal Title
  
  PloS One
  
  Volume: 11 Issue: 12 Pages: 1-15
- DOI
  10.1371/journal.pone.0167550
- NAID
  120005980901
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-16H05042, KAKENHI-PROJECT-26292168, KAKENHI-PROJECT-15K21080
[Journal Article] Visualization of the Epiblast 1 and Visceral Endodermal Cells using Fgf5-P2A-Venus BAC Transgenic Mice and Epiblast Stem Cells2016
- Author(s)
  Khoa LTP, Azami T, Tsukiyama T, Matsushita J, Tsukiyama-Fujii S, Takahashi S, Ema M.
- Journal Title
  
  PLoS One.
  
  Volume: 16(1) Issue: 7 Pages: 310-310
- DOI
  10.1371/journal.pone.0159246
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-26221004, KAKENHI-PROJECT-15K21080
[Journal Article] Generation of transgenic cynomolgus monkeys that express green fluorescent protein throughout the whole body2016
- Author(s)
  Seita Y, Tsukiyama T, Iwatani C, Tsuchiya H, Matsushita J, Azami T, Okahara J, Nakamura S, Hayashi Y, Hitoshi S, Itoh Y, Imamura T, Nishimura M, Tooyama I, Miyoshi H, Saitou M, Ogasawara K, Sasaki E, Ema M
- Journal Title
  
  Scientific Reports
  
  Volume: 2 Issue: 1 Pages: 24868-24868
- DOI
  10.1038/srep24868
- NAID
  130007023590
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-26640053, KAKENHI-PROJECT-15K21080, KAKENHI-PROJECT-16H04671, KAKENHI-PROJECT-26430070, KAKENHI-PROJECT-26870282, KAKENHI-PROJECT-16K14578
[Journal Article] A hyperactive <i>piggyBac</i> transposon system is an easy-to-implement method for introducing foreign genes into mouse preimplantation embryos2015
- Author(s)
  Suzuki S, Tsukiyama T, Kaneko T, Imai S, Minami N.
- Journal Title
  
  J. Reprod. Dev.
  
  Volume: 61 Issue: 3 Pages: 241-244
- DOI
  10.1262/jrd.2014-157
- NAID
  130005077740
- ISSN
  0916-8818, 1348-4400
- Language
  English
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-23380164, KAKENHI-PROJECT-25871115
[Journal Article] Generation of naive bovine induced pluripotent stem cells using piggyBac transposition of doxycycline-inducible transcription factors2015
- Author(s)
  Kawaguchi T., Tsukiyama T., Kimura K., Matsuyama S., Minami N., Yamada M. and Imai H.
- Journal Title
  
  PLoS ONE
  
  Volume: 10 Issue: 8 Pages: e0135403-e0135403
- DOI
  10.1371/journal.pone.0135403
- Peer Reviewed / Acknowledgement Compliant / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K21080, KAKENHI-PROJECT-24380172
[Journal Article] A modified EpiSC culture condition containing a GSK3 inhibitor can support germline-competent pluripotency in mice2014
- Author(s)
  Tsukiyama T, Ohinata Y
- Journal Title
  
  PLOS ONE
  
  Volume: 9 Issue: 4 Pages: e95329-e95329
- DOI
  10.1371/journal.pone.0095329
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-25712030, KAKENHI-PROJECT-25871115
[Journal Article] Establishment of trophoblast stem cells under defined culture conditions in mice2014
- Author(s)
  Ohinata Y, Tsukiyama T
- Journal Title
  
  PLOS ONE
  
  Volume: 9 Issue: 9 Pages: e107308-e107308
- DOI
  10.1371/journal.pone.0107308
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-25712030, KAKENHI-PROJECT-25871115
[Journal Article] A comprehensive system for generation and evaluation of induced pluripotent stem cells using piggyBac transposition2014
- Author(s)
  Tsukiyama T, Kato-Itoh M, Nakauchi H, Ohinata Y
- Journal Title
  
  PLOS ONE
  
  Volume: 9 Issue: 3 Pages: e92973-e92973
- DOI
  10.1371/journal.pone.0092973
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-25712030, KAKENHI-PROJECT-25871115
[Presentation] 遺伝子改変カニクイザルの作出2024
- Author(s)
  築山智之
- Organizer
  革新脳分科会The Final
- Invited
- Data Source
  KAKENHI-PROJECT-22K19246
[Presentation] 遺伝子改変カニクイザルの作出2024
- Author(s)
  築山智之
- Organizer
  革新脳分科会The Final
- Invited
- Data Source
  KAKENHI-PROJECT-23K23794
[Presentation] Generating transgenic non-human primates via the piggyBac transposon system.2023
- Author(s)
  Nakaya M. and Tsukiyama T.
- Organizer
  日本分子生物学会
- Data Source
  KAKENHI-PROJECT-22K19246
[Presentation] NPHP1ノックアウトカニクイザルの作成による若年性ネフロン癆の病態再現2023
- Author(s)
  鳥生直哉,中家雅隆,山田　龍,好川貴久,築山智之,柳田素子,伊佐　正
- Organizer
  日本内科学会講演会
- Data Source
  KAKENHI-PROJECT-23K27616
[Presentation] Generating transgenic non-human primates via the piggyBac transposon system.2023
- Author(s)
  Nakaya M. and Tsukiyama T.
- Organizer
  日本分子生物学会
- Data Source
  KAKENHI-PROJECT-23K23794
[Presentation] 遺伝子改変カニクイザルの作出2023
- Author(s)
  築山智之
- Organizer
  日本受精着床学会
- Invited
- Data Source
  KAKENHI-PROJECT-23K23794
[Presentation] piggyBacトランスポゾンシステムを介した非ヒト霊長類トランスジェニック動物の作出2023
- Author(s)
  中家雅隆, 築山智之
- Organizer
  日本繁殖生物学会
- Data Source
  KAKENHI-PROJECT-23K23794
[Presentation] 遺伝子改変カニクイザルの作出2023
- Author(s)
  築山智之
- Organizer
  日本受精着床学会
- Invited
- Data Source
  KAKENHI-PROJECT-22K19246
[Presentation] piggyBacトランスポゾンシステムを介した非ヒト霊長類トランスジェニック動物の作出2023
- Author(s)
  中家雅隆, 築山智之
- Organizer
  日本繁殖生物学会
- Data Source
  KAKENHI-PROJECT-22K19246
[Presentation] NPHP1ノックアウトカニクイザルの作成による若年性ネフロン癆の病態再現2022
- Author(s)
  鳥生直哉、中家雅隆、山田龍、好川貴久、岩谷千鶴、土屋英明、築山(藤井)勢津子、中川孝博、築山智之、柳田素子
- Organizer
  第65回日本腎臓学会学術総会
- Data Source
  KAKENHI-PROJECT-20H03697
[Presentation] piggyBacトランスポゾンシステムを利用したトランスジェニックカニクイザルの作出2022
- Author(s)
  中家雅隆, 築山智之
- Organizer
  日本繁殖生物学会
- Data Source
  KAKENHI-PROJECT-23K23794
[Presentation] piggyBacトランスポゾンシステムを利用したトランスジェニックカニクイザルの作出2022
- Author(s)
  中家雅隆, 築山智之
- Organizer
  日本繁殖生物学会
- Data Source
  KAKENHI-PROJECT-22K19246
[Presentation] piggyBacトランスポゾンシステムを利用したトランスジェニックカニクイザルの作出2022
- Author(s)
  中家雅隆, 築山智之
- Organizer
  日本繁殖生物学会
- Data Source
  KAKENHI-PLANNED-20H05763
[Presentation] Generation of transgenic cynomolgus monkeys using piggyBac transposition.2021
- Author(s)
  Tsukiyama T.
- Organizer
  日本分子生物学会
- Invited
- Data Source
  KAKENHI-PLANNED-20H05763
[Presentation] Generation of transgenic cynomolgus monkeys using piggyBac transposition.2021
- Author(s)
  Tsukiyama T.
- Organizer
  日本分子生物学会
- Invited
- Data Source
  KAKENHI-PROJECT-20K21370
[Presentation] CRISPR/Cas9を用いたカニクイザルにおける疾患モデリング2020
- Author(s)
  築山智之
- Organizer
  日本マーモセット研究会大会
- Invited
- Data Source
  KAKENHI-PROJECT-17K08135
[Presentation] Disease modeling in cynomolgus monkeys using CRISPR/Cas9.2020
- Author(s)
  Tsukiyama T.
- Organizer
  日本発生生物学会
- Invited
- Data Source
  KAKENHI-PROJECT-20K21370
[Presentation] Disease modeling in cynomolgus monkeys using CRISPR/Cas9.2020
- Author(s)
  Tsukiyama T.
- Organizer
  日本発生生物学会
- Invited
- Data Source
  KAKENHI-PROJECT-17K08135
[Presentation] Disease modeling in cynomolgus monkeys using CRISPR/Cas9.2020
- Author(s)
  Tsukiyama T.
- Organizer
  日本発生生物学会
- Invited
- Data Source
  KAKENHI-PLANNED-20H05763
[Presentation] CRISPR/Cas9を用いた遺伝性腎疾患モデルカニクイザルの作出2018
- Author(s)
  築山智之, 小林憲市, 中家雅隆, 岩谷千鶴, 土屋英明, 清田弥寿成, 松下淳, 北嶋郁, 河本育士, 中川孝博, 福田浩司, 岩切哲平, 和泉博之, 板垣伊織, 中村紳一朗, 河内明宏, 依馬正次
- Organizer
  日本分子生物学会
- Invited
- Data Source
  KAKENHI-PROJECT-17K08135
[Presentation] CRISPR/Cas9を用いたADPKDモデルカニクイザルの作出と解析2018
- Author(s)
  依馬正次, 築山智之, 小林憲市, 和泉博之, 河内明宏
- Organizer
  日本腎臓学会東部学術大会
- Invited
- Data Source
  KAKENHI-PROJECT-17K08135
[Presentation] カニクイザルを用いた遺伝性腎疾患動物モデルの作製2018
- Author(s)
  小林憲市, 築山智之, 影山進, 岩谷千鶴, 福田浩司, 岩切哲平, 和泉博之,中村紳一朗, 依馬正次, 河内明宏
- Organizer
  日本泌尿器科学会
- Data Source
  KAKENHI-PROJECT-17K08135
[Presentation] CRISPR/Cas9を用いた疾患モデルカニクイザルの作出2017
- Author(s)
  築山智之, 小林憲市, 岩谷千鶴, 土屋英明, 清田弥寿成, 松下淳, 北嶋郁, 河本育士, 中川孝博, 福田浩司, 岩切哲平, 和泉博之, 板垣伊織, 中村紳一朗, 河内明宏, 依馬正次
- Organizer
  生命科学系学会合同年次大会
- Data Source
  KAKENHI-PROJECT-17K08135
[Presentation] CRISPR/Cas9を用いた疾患モデルカニクイザルの作出2017
- Author(s)
  築山智之
- Organizer
  関西血管生物研究会
- Invited
- Data Source
  KAKENHI-PROJECT-17K08135
[Presentation] Disease modeling in cynomolgus monkeys using CRISPR/Cas9 injection into ICSI embryos2017
- Author(s)
  Tsukiyama T., Kobayashi K., Iwatani C., Tsuchiya H., Seita Y., Matsushita J., Kitajima K., Kawamoto I., Nakagawa T., Fukuda K., Iwakiri T., Izumi H., Itagaki I., Nakamura S., Kawauchi A. and Ema M.
- Organizer
  4th World Congress of Reproductive Biology
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K08135
[Presentation] Generation of GFP transgenic cynomolgus monkeys2016
- Author(s)
  Seita Y., Tsukiyama T., Iwatani C., Tsuchiya H., Matsushita J., Azami T., Ogasawara K. and Ema M.
- Organizer
  SRF Annual Conference 2016
- Place of Presentation
  University of Winchester
- Year and Date
  2016-07-11
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K21080
[Presentation] 全身でGFPを発現するトランスジェニックカニクイザルの作製2016
- Author(s)
  清田弥寿成、築山智之、岩谷千鶴、松下淳、土屋英明、佐々木えりか、依馬正次
- Organizer
  日本繁殖生物学会
- Place of Presentation
  麻布大学
- Year and Date
  2016-09-12
- Data Source
  KAKENHI-PROJECT-15K21080
[Presentation] 全身でGFPを発現するトランスジェニックカニクイザルの作製2016
- Author(s)
  清田弥寿成、築山智之、岩谷千鶴、松下淳、土屋英明、浅見拓哉、佐々木えりか、依馬正次
- Organizer
  日本分子生物学会
- Place of Presentation
  パシフィコ横浜
- Year and Date
  2016-11-30
- Data Source
  KAKENHI-PROJECT-15K21080
[Presentation] マウスおよびカニクイザル多能性幹細胞におけるCRISPR/Cas9を用いた発現増幅型多能性幹細胞レポーターのノックイン2015
- Author(s)
  築山智之、丹羽　仁史、岩谷千鶴、土屋英明、清田弥寿成、松下淳、依馬正次
- Organizer
  日本繁殖生物学会
- Place of Presentation
  宮崎大学
- Year and Date
  2015-09-19
- Data Source
  KAKENHI-PROJECT-15K21080
[Presentation] ヒトおよびカニクイザル多能性幹細胞の培養におけるWnt阻害剤の影響2014
- Author(s)
  築山智之、岩谷千鶴、土屋英明、清田弥寿成、松下淳、依馬正次
- Organizer
  日本繁殖生物学会
- Place of Presentation
  帯広畜産大学
- Year and Date
  2014-08-23
- Data Source
  KAKENHI-PROJECT-25871115
[Presentation] Germline contribution of rat iPS cells in mouse-rat interspecific chimeric animals.2014
- Author(s)
  Ohinata Y. and Tsukiyama T.
- Organizer
  日本繁殖生物学会
- Place of Presentation
  帯広畜産大学
- Year and Date
  2014-08-21
- Data Source
  KAKENHI-PROJECT-25871115
[Presentation] piggyBacトランスポゾンを用いたリプログラミング因子導入によるウシ羊膜細胞からの栄養膜細胞株樹立の試み2014
- Author(s)
  川口高正、築山智之、松山秀一、木村康二、南直治郎、山田雅保、今井裕
- Organizer
  日本繁殖生物学会
- Place of Presentation
  帯広畜産大学
- Year and Date
  2014-08-21
- Data Source
  KAKENHI-PROJECT-25871115
[Presentation] piggyBacトランスポゾンを用いたナイーブ型ウシiPS細胞の樹立の試み2013
- Author(s)
  川口高正、築山智之、南直治郎、山田雅保、松山秀一、木村康二、今井裕
- Organizer
  日本繁殖生物学会
- Place of Presentation
  東京農工大学
- Data Source
  KAKENHI-PROJECT-25871115
[Presentation] 蛍光レポーターによるPGC形成過程、精子形成過程、卵形成過程の可視化2013
- Author(s)
  大日向康秀、築山智之
- Organizer
  日本繁殖生物学会
- Place of Presentation
  東京農工大学
- Data Source
  KAKENHI-PROJECT-25871115

1. Yanagita Motoko (70378769)

# of Collaborated Projects: 2 results

# of Collaborated Products: 2 results
2. 高島康弘 (70469930)

# of Collaborated Projects: 2 results

# of Collaborated Products: 0 results
3. Itoh Yasushi (90324566)

# of Collaborated Projects: 1 results

# of Collaborated Products: 1 results
4. Saitou Mitinori (80373306)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
5. 依馬正次 (60359578)

# of Collaborated Projects: 1 results

# of Collaborated Products: 3 results
6. 石垣宏仁 (90432301)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
7. 中村紳一朗 (50307980)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
8. 柊卓志 (00512477)

# of Collaborated Projects: 1 results

# of Collaborated Products: 1 results
9. 伊佐正 (20212805)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
10. 太田淳 (80304161)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
11. 大串雅俊 (00462664)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
12. TSUCHIYA Hideaki

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
13. MIZUTANI Kenichi

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
14. 小林憲市

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
15. 岡本郁弘

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
16. 中家雅隆

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
17. 南直治郎

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
18. 大日向康秀

# of Collaborated Projects: 0 results

# of Collaborated Products: 4 results
19. 高橋智

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
20. 扇田久和

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
21. 西村正樹

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
22. 漆谷真

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
23. 守村敏史

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
24. 小笠原一誠

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
25. 佐々木えりか

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results

Tsukiyama Tomoyuki 築山 智之

TSUKIYAMA Tomoyuki 築山 智之

Research Projects

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モザイク性の解消による早期表現型解析のための遺伝子改変技術基盤の構築Principal Investigator

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能動的組換えによる革新的遺伝子ノックインPrincipal Investigator

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Development of innovative knock-in technology by active gene transferPrincipal Investigator

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遺伝子改変動物作出におけるモザイク性の解消Principal Investigator

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Development of innovative knock-in technology using endogenous homologous recombination mechanismPrincipal Investigator

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Establishment of a technology platform for genetic modification of fertilized eggs that enables analysis from the F0 generationPrincipal Investigator

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Tsukiyama Tomoyuki 築山智之

TSUKIYAMA Tomoyuki 築山智之

[Book] 実験医学別冊マウス表現型解析スタンダード2016