Kuno Akihiro 久野朗広

Researcher Number	60830122
Other IDs
Affiliation (Current)	2025: 筑波大学, 医学医療系, 研究員
Affiliation (based on the past Project Information) *help	2024: 筑波大学, 医学医療系, 研究員 2019 – 2024: 筑波大学, 医学医療系, 助教
Review Section/Research Field	Principal Investigator Basic Section 42040:Laboratory animal science-related / Basic Section 43060:System genome science-related Except Principal Investigator Medium-sized Section 42:Veterinary medical science, animal science, and related fields / Basic Section 42040:Laboratory animal science-related / Basic Section 43010:Molecular biology-related / Basic Section 43060:System genome science-related / Basic Section 43050:Genome biology-related
Keywords	Principal Investigator 遺伝型解析 / 深層生成モデル / 次世代シーケンサー / CRISPR-Cas / 実験動物学 / ゲノム編集 / バイオインフォマティクス Except Principal Investigator ゲノム編集 / 遺伝子改変マウス / 量依存性 … More / 転写因子 / エピジェネティクス / Klf4 / iPS細胞 / 必須遺伝子 / モデルマウス開発 / エピゲノム編集 / 精密ゲノム編集 / CRISPR-Cas3 / ランダムミュータジェネシス / マウスES細胞 / ランダム変異 / Nanog / X染色体再活性化 / KLF4 / 転写 / エピゲノム / 多能性 / 転写制御 / スプライシング関連タンパク / RNA結合タンパク / マウス / 原腸胚 / 胚盤胞補完法 / 選択的スプライシング / 原腸胚形成 / 胚発生 / マウス遺伝学 / Cre-LoxP / モデル動物作製 / Long Read Sequence / Creリソース / 遺伝型解析 / Creマウス / ロングリードシークエンス Less

Development of precise genome engineering methods using modiied CRISPR-Cas3
- Principal Investigator
  
  吉見一人
- Project Period (FY)
  2024 – 2027
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 43060:System genome science-related
- Research Institution
  The University of Tokyo
Development of the nCATS-DAJIN method for ultra-high precision genotyping in genome-edited animalsPrincipal Investigator
- Principal Investigator
  
  久野朗広
- Project Period (FY)
  2024 – 2025
- Research Category
  
  Grant-in-Aid for Early-Career Scientists
- Review Section
  
  Basic Section 42040:Laboratory animal science-related
- Research Institution
  University of Tsukuba
Cell fate decision by dose of transcription factors
- Principal Investigator
  
  西村健
- Project Period (FY)
  2023 – 2025
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 43010:Molecular biology-related
- Research Institution
  University of Tsukuba
新規direct-cKOシステムでの必須遺伝子探索とその網羅的解析基盤の構築
- Principal Investigator
  
  水野聖哉
- Project Period (FY)
  2022 – 2024
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 42040:Laboratory animal science-related
- Research Institution
  University of Tsukuba
Mutagenesis of non-coding regions via excisional genome editing
- Principal Investigator
  
  Mizuno Seiya
- Project Period (FY)
  2021 – 2022
- Research Category
  
  Grant-in-Aid for Challenging Research (Exploratory)
- Review Section
  
  Medium-sized Section 42:Veterinary medical science, animal science, and related fields
- Research Institution
  University of Tsukuba
Development of a universal genome-editing genotyping method using a deep generation modelPrincipal Investigator
- Principal Investigator
  
  Kuno Akihiro
- Project Period (FY)
  2020 – 2021
- Research Category
  
  Grant-in-Aid for Early-Career Scientists
- Review Section
  
  Basic Section 43060:System genome science-related
- Research Institution
  University of Tsukuba
Biological functions of alternative splicing on mouse gastrulation
- Principal Investigator
  
  Sugiyama Fumihiro
- Project Period (FY)
  2020 – 2023
- Research Category
  
  Grant-in-Aid for Scientific Research (A)
- Review Section
  
  Medium-sized Section 42:Veterinary medical science, animal science, and related fields
- Research Institution
  University of Tsukuba
Establishment of new gene modified moue production system
- Principal Investigator
  
  Mizuno Seiya
- Project Period (FY)
  2019 – 2021
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 42040:Laboratory animal science-related
- Research Institution
  University of Tsukuba
Molecular dynamics of epigenomic transcriptional regulation by Klf4 in iPSC generation
- Principal Investigator
  
  Nishimura Ken
- Project Period (FY)
  2019 – 2021
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 43050:Genome biology-related
- Research Institution
  University of Tsukuba

All 2023 2022 2021 2020 2019

All Journal Article Presentation

[Journal Article] Early reactivation of clustered genes on the inactive X chromosome during somatic cell reprogramming2022
- Author(s)
  Aizawa Shiho、Nishimura Ken、Mondejar Gonzalo Seminario、Kumar Arun、Bui Phuong Linh、Tran Yen Thi Hai、Kuno Akihiro、Muratani Masafumi、Kobayashi Shin、Nabekura Tsukasa、Shibuya Akira、Sugihara Eiji、Sato Taka-Aki、Fukuda Aya、Hayashi Yohei、Hisatake Koji
- Journal Title
  
  Stem Cell Reports
  
  Volume: 17 Issue: 1 Pages: 53-67
- DOI
  10.1016/j.stemcr.2021.11.008
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-21H02678, KAKENHI-PROJECT-19H03203
[Journal Article] Downregulation of Odd-Skipped Related 2, a Novel Regulator of Epithelial-Mesenchymal Transition, Enables Efficient Somatic Cell Reprogramming2022
- Author(s)
  Anh Le Phuong Hoang、Nishimura Ken、Kuno Akihiro、Linh Nguyen Thuy、Kato Tetsuo、Ohtaka Manami、Nakanishi Mahito、Sugihara Eiji、Sato Taka-Aki、Hayashi Yohei、Fukuda Aya、Hisatake Koji
- Journal Title
  
  Stem Cells
  
  Volume: 40 Issue: 4 Pages: 397-410
- DOI
  10.1093/stmcls/sxac012
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21H02678, KAKENHI-PROJECT-19H03203
[Journal Article] DAJIN enables multiplex genotyping to simultaneously validate intended and unintended target genome editing outcomes2022
- Author(s)
  Kuno Akihiro, Ikeda Yoshihisa, Ayabe Shinya, et. al.
- Journal Title
  
  PLOS Biology
  
  Volume: 20 Issue: 1 Pages: e3001507-e3001507
- DOI
  10.1371/journal.pbio.3001507
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-20K15775, KAKENHI-PROJECT-21H04838, KAKENHI-PROJECT-19H03142
[Journal Article] Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency2022
- Author(s)
  Borisova Evgeniia、Nishimura Ken、An Yuri、Takami Miho、Li Jingyue、Song Dan、Matsuo-Takasaki Mami、Luijkx Dorian、Aizawa Shiho、Kuno Akihiro、Sugihara Eiji、Sato Taka-aki、Yumoto Fumiaki、Terada Tohru、Hisatake Koji、Hayashi Yohei
- Journal Title
  
  iScience
  
  Volume: 25 Issue: 1 Pages: 103525-103525
- DOI
  10.1016/j.isci.2021.103525
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-21H02678, KAKENHI-PROJECT-19H03203
[Journal Article] DAJIN-assisted multiplex genotyping to validate the outcomes of CRISPR-Cas-based genome editing2020
- Author(s)
  Kuno, A., Ikeda, Y., Ayabe, S., Kato, K., Sakamoto, K., Suzuki, S., ... & Mizuno, S.
- Journal Title
  
  bioRxiv
  
  Volume: -
- DOI
  10.1101/2020.12.14.422641
- Open Access
- Data Source
  KAKENHI-PROJECT-20K15775
[Presentation] 生殖隔離を制御する染色体領域の機能解析に資するセミデザイン型ランダム変異マウス胚性幹細胞ライブラリーの構築2023
- Author(s)
  水野聖哉、森本健斗、久野朗広、杉山文博
- Organizer
  先端モデル動物支援プラットフォーム令和4年度成果発表会
- Data Source
  KAKENHI-PROJECT-21K19178
[Presentation] リプログラム因子のパイオニア因子としての機能と量依存性2022
- Author(s)
  西村健、久野朗広、久武幸司
- Organizer
  第21回日本再生医療学会総会
- Invited
- Data Source
  KAKENHI-PROJECT-19H03203
[Presentation] DAJIN validates the multiallelic genome-editing outcomes using machine learning-assisted long-read sequencing2021
- Author(s)
  久野朗広
- Organizer
  第10回生命医薬情報学連合大会（IIBMP2021）
- Data Source
  KAKENHI-PROJECT-20K15775
[Presentation] 『より良い』ノックアウトマウスを作出するためのゲノム編集デザイン2019
- Author(s)
  水野　聖哉、久野朗広、高橋智、杉山文博
- Organizer
  第66回日本実験動物学会総会
- Invited
- Data Source
  KAKENHI-PROJECT-19H03142

1. Mizuno Seiya (10633141)

# of Collaborated Projects: 4 results

# of Collaborated Products: 2 results
2. Nishimura Ken (80500610)

# of Collaborated Projects: 2 results

# of Collaborated Products: 4 results
3. 福田綾 (50436276)

# of Collaborated Projects: 2 results

# of Collaborated Products: 2 results
4. 林洋平 (90780130)

# of Collaborated Projects: 2 results

# of Collaborated Products: 0 results
5. 綾部信哉 (10633563)

# of Collaborated Projects: 2 results

# of Collaborated Products: 0 results
6. Sugiyama Fumihiro (90226481)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
7. 藤山知之 (00635089)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
8. 村田知弥 (60713485)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
9. 吉見一人 (50709813)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
10. 竹下浩平 (80346808)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
11. 久武幸司

# of Collaborated Projects: 0 results

# of Collaborated Products: 3 results

Kuno Akihiro 久野 朗広

Research Projects

Research Products

Co-Researchers

Development of precise genome engineering methods using modiied CRISPR-Cas3

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Development of the nCATS-DAJIN method for ultra-high precision genotyping in genome-edited animalsPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Cell fate decision by dose of transcription factors

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

新規direct-cKOシステムでの必須遺伝子探索とその網羅的解析基盤の構築

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Mutagenesis of non-coding regions via excisional genome editing

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Development of a universal genome-editing genotyping method using a deep generation modelPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Biological functions of alternative splicing on mouse gastrulation

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Establishment of new gene modified moue production system

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Molecular dynamics of epigenomic transcriptional regulation by Klf4 in iPSC generation

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

[Journal Article] Early reactivation of clustered genes on the inactive X chromosome during somatic cell reprogramming2022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Downregulation of Odd-Skipped Related 2, a Novel Regulator of Epithelial-Mesenchymal Transition, Enables Efficient Somatic Cell Reprogramming2022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] DAJIN enables multiplex genotyping to simultaneously validate intended and unintended target genome editing outcomes2022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency2022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] DAJIN-assisted multiplex genotyping to validate the outcomes of CRISPR-Cas-based genome editing2020

Author(s)

Kuno Akihiro 久野朗広