SAKANO Yoshiyuki 坂野好幸

Researcher Number	70014959
Other IDs
External Links
Affiliation (based on the past Project Information) *help	2005: Tokyo University of Agriculture & Technology, Institute of Symbiotic Science and Technology, Professor, 大学院・共生科学技術研究部, 教授 2004 – 2005: 東京農工大学, 大学院・共生科学技術研究部, 教授 2000 – 2003: 東京農工大学, 農学部, 教授 1992 – 1995: 東京農工大学, 農学部, 教授 1989 – 1991: 東京農工大学, 農学部, 助教授
Review Section/Research Field	Principal Investigator Applied molecular and cellular biology / 製造化学・食品 Except Principal Investigator Structural biochemistry / Structural biochemistry / 応用微生物学・応用生物化学 / 代謝生物化学
Keywords	Principal Investigator TVA / pullulanase / Bacillus acidopullulyticus / Protein crystallography / Isopullulanase / α-amylase / Cyclodextrin / Pullulan / 結構構造解析 / パノシルパノース … More / 結晶構造解析 / イソプルラナーゼ / α-アミラーゼ / サイクロデキストリン / プルラン / sugar alcohol / ceramics / inclusion / branched cyclodxtrin / immobilization / reactor / マルトトリイト-ル / ゲスト化合物 / セラミックス担体 / <Bacillus acidopullulyticus>___ー / イソエリ-ト / セラミックス / 包接作用 / ポリオ-ル / 分岐サイクロデキストリン / 固定化酵素リアクタ- … More Except Principal Investigator mutant enzyme / cyclodextrin / X-ray structure / 好熱性放線菌 / ミュータント酵素 / シクロデキストリン / X線結晶解析 / Thermoactinomyces vulgaris R47 / 放射光 / glucoside linkage / hydrolysis / グルコシド結合 / 加水分解 / α-アミラーゼ / synchrotron radiation / metabolism / oligosaccharide / transporter / 代謝系 / オリゴ糖 / 膜間輸送 / kinetic parameters / α-amylase / 反応動力学パラメーター / Thermoactinomvces vulgaris / substrate-recognition / α-amvlase / 基質認識 / Cyclodextrin synthase / Glucosidase / Amylase / Utilization of carbohydrase / Mechanism of substrate confirmation / Carbohydrate-Synthase / Carbohydrate-hydrolase / グリコシダーゼ / サイクロデキストリン合成酵素 / グルコシダーゼ / アミラーゼ / 糖質分解酵素の高度利用 / 糖認識機構 / 糖質合成酵素 / 糖質分解酵素 / beta-galactoside / Natural antibody / Tumour cell / Immunoglobuln / 抗βーガラクトシル基 / 抗ーαーガラクトシル基 / α-ガラクトシド / がん / β-ガラクトシド / 自然抗体 / がん細胞 / 免疫グロブリン Less

Structural Study of Thermoactinomyces vulgaris R-47 Cyclodextrin Transport and Metabolic Pathway
- Principal Investigator
  
  KAMITORI Shigehiro
- Project Period (FY)
  2004 – 2005
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  Structural biochemistry
- Research Institution
  Kagawa University
Study of the cyclodextrin hydrolyzing mechanism of Thermoactinomyces vulgaris R47 α-amylases based on X-ray structures
- Principal Investigator
  
  KAMITORI Shigehiro
- Project Period (FY)
  2002 – 2003
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Structural biochemistry
- Research Institution
  Tokyo University Agriculture Arid Technology
X-ray crystallography and protein engineering of branched oligosaccharides-producing enzymesPrincipal Investigator
- Principal Investigator
  
  SAKANO Yoshiyuki
- Project Period (FY)
  2000 – 2002
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  Applied molecular and cellular biology
- Research Institution
  Tokyo University of Agriculture and Technology
Study on Substrates-Recognition Mechanism of Thermoactinomyces vulgaris R-47 α-Amylase 2 (TVAII) based on X-ray Structures.
- Principal Investigator
  
  KAMITORI Shigehiro
- Project Period (FY)
  2000 – 2001
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Structural biochemistry
- Research Institution
  Tokyo University of Agriculture & Technology
Studies on Mechanism of Substrate Confirmation, Modification of Function, and Utilization for Carbohydrate-Hydrolase and Synthase
- Principal Investigator
  
  CHIBA Seiya
- Project Period (FY)
  1993 – 1995
- Research Category
  
  Grant-in-Aid for Co-operative Research (A)
- Research Field
  
  応用微生物学・応用生物化学
- Research Institution
  HOKKAIDO UNIVERSITY
Studies on the cell-binding immunoglobulin M in human plasma
- Principal Investigator
  
  FUJIMOTO Daisaburo
- Project Period (FY)
  1991 – 1993
- Research Category
  
  Grant-in-Aid for General Scientific Research (B)
- Research Field
  
  代謝生物化学
- Research Institution
  Tokyo University of Agriculture and Technology
Development and Application of Successive Production of Branched Cyclodextrins Using a ReactorPrincipal Investigator
- Principal Investigator
  
  SAKANO Yoshiyuki
- Project Period (FY)
  1989 – 1990
- Research Category
  
  Grant-in-Aid for Developmental Scientific Research (B).
- Research Field
  
  製造化学・食品
- Research Institution
  Tokyo University of Agriculture and Technology, Faculty of Agriculture

All 2006 2005 2004

All Journal Article

[Journal Article] Structure of a Complex of Thermoactinomyces vulgaris R-47 α-Amylase 2 with Maltohexaose Demonstrates the Important Role of Aromatic Residues at the Reducing End of the Substrate Binding Cleft.2006
- Author(s)
  Ohtaki, A., Mizuno, M., Yoshida, H., Tonozuka, T., Sakano, Y., Kamitori, S.
- Journal Title
  
  Carbohydrate Research (印刷中)
- Data Source
  KAKENHI-PROJECT-16370048
[Journal Article] Structure of a Complex of Thermoactinomyces vulgaris R-47 α-Amylase 2 with Maltohexaose Demonstrates the Important Role of Aromatic Residues at the Reducing End of the Substrate Binding Cleft.2006
- Author(s)
  Ohtaki, A., Mizuno, M., Yoshida, H., Tonozuka, T., Sakano, Y., Kamitori, S.
- Journal Title
  
  Carbohydr. Res. 341
  
  Pages: 1041-1046
- Description
  「研究成果報告書概要(和文)」より
- Data Source
  KAKENHI-PROJECT-16370048
[Journal Article] Structure of a Complex of Thermoactinomyces vulgaris R-47 α-Amylase 2 with Maltohexaose Demonstrates the Important Role of Aromatic Residues at the Reducing End of the Substrate Binding Cleft.2006
- Author(s)
  Ohtaki, A., Mizuno, M., Yoshida, H., Tonozuka, T., Sakano, Y., Kamitori, S.
- Journal Title
  
  Carbohydr.Res. 341
  
  Pages: 1041-1046
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-16370048
[Journal Article] Mutagenesis and structural analysis of Thermoactinomyces vulgaris R-47 α-amylase II (TVAII).2005
- Author(s)
  Mizuno, M., Ichikawa, K., Tonozuka, T, Ohtaki, A., Shimura, Y., Kamitori, S., Nishikawa, A., Sakano, Y.
- Journal Title
  
  J.Appl.Glycosci. 52
  
  Pages: 225-231
- NAID
  10015600404
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-16370048
[Journal Article] Mutagenesis and structural analysis of Thrmoactinomyces vulgaris R-47 α-amylase II (TVA II)2005
- Author(s)
  Mizuno, M., Ichikawa, K., Tonozuka, T., Ohtaki, A., Shimura, Y., Kamitori, S., Nishikawa, A., Sakano, Y.
- Journal Title
  
  J. Appl. Glycosci. 52
  
  Pages: 225-231
- Description
  「研究成果報告書概要(和文)」より
- Data Source
  KAKENHI-PROJECT-16370048
[Journal Article] Complexes of Thermoactinomyces vulgaris R-47 α-amylase 1 and pullulan model oligossacharides provide new insight into the mechanism for recognizing substrates with α-(1,6) glycosidic linkages.2005
- Author(s)
  Abe, A., Yoshida, H., Tonozuka, T., Sakano, Y., Kamitori, S.
- Journal Title
  
  FEBS J. 272
  
  Pages: 6145-6153
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-16370048
[Journal Article] Crystallization and preliminary X-ray analysis of Thermoactinomyces vulgaris R-47 maltooligosaccharide-metabolizing enzyme homologous to glucoamylase2005
- Author(s)
  Ichikawa, K., Tonozuka, T., Mizuno, M., Tanabe, Y., Kamitori, S., Nishikawa, A., Sakano, Y.
- Journal Title
  
  Acta Crystallogr.Sect F 61
  
  Pages: 302-304
- Data Source
  KAKENHI-PROJECT-16370048
[Journal Article] Complexes of Thermoactinomyces vulgaris R-47 α-amylase 1 and pullulan model oligossacharides provide new insight into the mechanism for recognizing substrates with α-(1, 6) glycosidic linkages.2005
- Author(s)
  Abe, A., Yoshida, H., Tonozuka, T., Sakano, Y., Kamitori, S.
- Journal Title
  
  FEBS J. 272
  
  Pages: 6145-6153
- Description
  「研究成果報告書概要(和文)」より
- Data Source
  KAKENHI-PROJECT-16370048
[Journal Article] Crystallization and preliminary X-ray analysis of Thermoactinomyces vulgaris R-47 maltooligosaccharide-metabolizing enzyme homologous to glucoamylase2005
- Author(s)
  Ichikawa, K., Tonozuka, T., Mizuno, M., Tanabe, Y., Kamitori, S., Nishikawa, A., Sakano, Y.
- Journal Title
  
  Acta Crystallogr. F61
  
  Pages: 302-304
- Description
  「研究成果報告書概要(和文)」より
- Data Source
  KAKENHI-PROJECT-16370048
[Journal Article] Complex structures of Thermoactinomyces vulgaris R-47 α-amylase 2 with acarbose and cyclodextrins demonstrate the multiple substrate recognition mechanism.2004
- Author(s)
  Ohtaki, A., Mizuno, M., Tonozuka, T., Sakano, Y., Kamitori, S.
- Journal Title
  
  J. Biol. Chem. 279
  
  Pages: 31033-31040
- Description
  「研究成果報告書概要(和文)」より
- Data Source
  KAKENHI-PROJECT-16370048
[Journal Article] Complex structures of Thermoactinomyces vulgaris R-47 α-amylase 2 with acarbose and cyclodextrins demonstrate the multiple substrate recognition mechanism.2004
- Author(s)
  Ohtaki, A., Mizuno, M., Tonozuka, T., Sakano, Y., Kamitori, S.
- Journal Title
  
  J Biol.Chem. 279
  
  Pages: 31033-31040
- Data Source
  KAKENHI-PROJECT-16370048
[Journal Article] Complex structures of Thermoactinomyces vulgaris R-47 α-amylase 2 with acarbose and cyclodextrins demonstrate the multiple substrate recognition mechanism.2004
- Author(s)
  Ohtaki, A., Mizuno, M., Tonozuka, T., Sakano, Y., Kamitori, S.
- Journal Title
  
  J.Biol.Chem. 279
  
  Pages: 31033-31040
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-16370048

1. KAMITORI Shigehiro (00262246)

# of Collaborated Projects: 4 results

# of Collaborated Products: 12 results
2. FUJIMOTO Daisaburo (40004288)

# of Collaborated Projects: 2 results

# of Collaborated Products: 0 results
3. CHIBA Seiya (30001449)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
4. HIZUKURI Susumu (90041590)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
5. MINAMIURA Noshi (20047129)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
6. SUZUKI Yuzuru (50046471)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
7. YAMANE Kunio (20013336)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
8. SAKAI Hiroshi (10092214)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
9. NISHIKAWA Atsushi (30218127)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
10. TONOZUKA Takashi (50285194)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
11. YOSHIDA Hiromi (10313305)

# of Collaborated Projects: 1 results

# of Collaborated Products: 5 results
12. KUSANO Shuーichi

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
13. SHIRAISHI Takanori

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
14. KOGURE Yasuo

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
15. 白石尊憲

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
16. 小暮靖夫

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results

SAKANO Yoshiyuki 坂野 好幸

Research Projects

Research Products

Co-Researchers

Structural Study of Thermoactinomyces vulgaris R-47 Cyclodextrin Transport and Metabolic Pathway

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Study of the cyclodextrin hydrolyzing mechanism of Thermoactinomyces vulgaris R47 α-amylases based on X-ray structures

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

X-ray crystallography and protein engineering of branched oligosaccharides-producing enzymesPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Study on Substrates-Recognition Mechanism of Thermoactinomyces vulgaris R-47 α-Amylase 2 (TVAII) based on X-ray Structures.

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Studies on Mechanism of Substrate Confirmation, Modification of Function, and Utilization for Carbohydrate-Hydrolase and Synthase

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Studies on the cell-binding immunoglobulin M in human plasma

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Development and Application of Successive Production of Branched Cyclodextrins Using a ReactorPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

[Journal Article] Structure of a Complex of Thermoactinomyces vulgaris R-47 α-Amylase 2 with Maltohexaose Demonstrates the Important Role of Aromatic Residues at the Reducing End of the Substrate Binding Cleft.2006

Author(s)

Journal Title

Data Source

[Journal Article] Structure of a Complex of Thermoactinomyces vulgaris R-47 α-Amylase 2 with Maltohexaose Demonstrates the Important Role of Aromatic Residues at the Reducing End of the Substrate Binding Cleft.2006

Author(s)

Journal Title

Description

Data Source

[Journal Article] Structure of a Complex of Thermoactinomyces vulgaris R-47 α-Amylase 2 with Maltohexaose Demonstrates the Important Role of Aromatic Residues at the Reducing End of the Substrate Binding Cleft.2006

Author(s)

Journal Title

Description

Data Source

[Journal Article] Mutagenesis and structural analysis of Thermoactinomyces vulgaris R-47 α-amylase II (TVAII).2005

Author(s)

Journal Title

NAID

Description

Data Source

[Journal Article] Mutagenesis and structural analysis of Thrmoactinomyces vulgaris R-47 α-amylase II (TVA II)2005

Author(s)

Journal Title

Description

Data Source

[Journal Article] Complexes of Thermoactinomyces vulgaris R-47 α-amylase 1 and pullulan model oligossacharides provide new insight into the mechanism for recognizing substrates with α-(1,6) glycosidic linkages.2005

Author(s)

Journal Title

Description

Data Source

[Journal Article] Crystallization and preliminary X-ray analysis of Thermoactinomyces vulgaris R-47 maltooligosaccharide-metabolizing enzyme homologous to glucoamylase2005

Author(s)

Journal Title

Data Source

SAKANO Yoshiyuki 坂野好幸