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YOSHIHARA Eisaku  良原 栄策

ORCIDConnect your ORCID iD *help
… Alternative Names

石井 純子  イシイ ジュンコ

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Researcher Number 70167063
Other IDs
Affiliation (based on the past Project Information) *help 2011: 東海大学, 医学部, 准教授
2007 – 2008: Tokai University, 医学部, 准教授
1993 – 2006: 東海大学, 医学部, 助教授
2005: Tokai University, Molecular Life Science, Associate Professor, 総合医学研究所, 助教授
1993: 東海大学, 医学部, 研究員 … More
1990 – 1992: 東海大学, 医学部, 講師
1988: 東海大学, 医学部・細胞情報科学, 助手
1986 – 1988: 東海大学, 医学部, 助手 Less
Review Section/Research Field
Principal Investigator
Bacteriology (including Mycology) / 細菌学 / Bacteriology (including Mycology)
Except Principal Investigator
Bacteriology (including Mycology) / 細菌学 / Biological Sciences / Structural biochemistry / Applied genomics
Keywords
Principal Investigator
緑膿菌 / 外膜 / Pseudomonas aeruginosa / protein D2 / 多剤耐性 / MexAB-OprM / ポーリン / 薬剤排出ポンプ / protease / porin … More / プロテアーゼ / モノクローナル抗体 / 阻害剤 / 多剤排出ポンプ / チャンネル蛋白 / inhibitors / 院内感染 / FRET / multidrug efflux pump / multidrug resistance / OprM / OprE3 / OprD / 触媒基 / 部位特異的変異 / チャネル / 細胞外ループ / ポ-リン / monoclonal antibodies / extracellular loops / oligomeric structure / outer membrane channel / 薬剤感受性 / 阻害物質 / 外服チャネル / オリゴマー構造 / 外膜チャネル / protein-protein interaction / subunit / MexAB-OprM pump / drug efflux pump / HlyBD-TolC / アフィニティカラム / ヒスチジンタグ / タンパク間相互作用 / サブユニット / MexAB-OprMポンプ / maltoporin / membrane topology / α-helix / channel / tolC / トポロジー決定法 / マルトポーリン / 膜トポロジー / α-ヘリックス / チャンネル / protein crystal / nucleotide sequence / catalytic residues / 部位特異的変異導入 / 塩基配列 / タンパク質結晶 / 全塩基配列 / mutifunctional protein / catalytic residue / site-directed mutagenesis / outer membrane protein / 塩基性アミノ酸 / ptoteinD2 / 多機能タンパク質 / proteinD2 / 外膜タンパク質 / imipenem / gating / channel protein / outer membrane / 調節因子 / Ca^<2+>イオン / OprD2 / イミペネム / ゲート開閉 / 薬剤耐性 / ポーリン蛋白 / 物質透過性 / ポンプ阻害剤 / ペプチド抗原 / 多剤耐性化 / 感染症 / ペプチド / 抗体 / 開閉調節 / カルシウム / ゲート / 孔開閉 / ドメイン構造 / チャンネル蛋白質 / ドメイン / 分子集合 … More
Except Principal Investigator
薬剤耐性 / 緑膿菌 / 抗生物質 / 膜蛋白 / 外膜 / 排出 / Membrane protein / 細菌 / 耐性 / 異物 / ポンプ / 人工膜 / 透過性 / 透過孔 / bacteria / antibiotic / transporter / RND / MexB / 膜貫通領域 / 排出ポンプ / トランスポーター / 薬物 / 院内感染 / Efflux / Pump / 異物排出 / 細胞膜 / 輸送 / トポロジー / Antibiotic resistance / Pseudomonas / 膜蛋白質 / ポーリン / Reconstitution / Drug resistance / Outer membrane / ポ-リン / 日和見感染 / 排除限界 / pore / barrier / permeability / opportunistic infection / gram-negative pathogen / outer membrane / 孔 / 細菌膜 / cell membrane / membrane protein / Psudomonas / xenobiotic antibiotic / efflux pump / drug resistance / 変異体 / エネルギー / X線解析 / 結晶構造 / 抗生物質・異物 / membrane proteins / drug / resistance / pump / efflux / 多剤耐性 / RND-ファミリー / MexAB-OprM / 基質認識 / Infection / X-ray diffraction / Crystal / Antibiotics / X線回析 / X-線結晶回析 / X-線結晶解析 / 結晶 / Transport / Membrane / Membrane topology / 生体防御 / Channel / Porin / Lipid bilayr / Pseudomonas aeruginosa / 脂質平面膜 / イミペネム / OprD2 / チャンネル / Cloning / Planar membrane / Liposome / Permeability / リポソ-ム / 再構成 / クロ-ニング / 平面膜 / エンドサイトーシス / 神経伝達物質 / シナプス小胞 / ゲノムワイド相関解析(GWAS) / 酵母ツーハイブリッド法 / シンタキシン / シナプトガミン / NETO / シナプトタグミン / コンタクチン / 疾患モデルマウス / 感受性遺伝子 / GWAS / ネットワーク解析 / 相関解析 / マイクロサテライト / 摂食障害 Less
  • Research Projects

    (20 results)
  • Research Products

    (22 results)
  • Co-Researchers

    (15 People)
  •  Genome-wide identification of genes for anorexia and their cascade analysis on disease development by functional study

    • Principal Investigator
      INOKO Hidetoshi
    • Project Period (FY)
      2007 – 2011
    • Research Category
      Grant-in-Aid for Scientific Research (S)
    • Research Field
      Applied genomics
    • Research Institution
      Tokai University
  •  Development of antibodies and small molecules as inhibitors ofPseudomonas aeruginosa multidrug efflux pumpsPrincipal Investigator

    • Principal Investigator
      YOSHIHARA Eisaku
    • Project Period (FY)
      2007 – 2008
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      Bacteriology (including Mycology)
    • Research Institution
      Tokai University
  •  Discovery of the extracellular loops being essential for the proper function of the multidrug efflux pump suggests that the loops may be an excellent target for the pump inhibitorPrincipal Investigator

    • Principal Investigator
      YOSHIHARA Eisaku
    • Project Period (FY)
      2005 – 2006
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      Bacteriology (including Mycology)
    • Research Institution
      Tokai University
  •  The elucidation of the molecular assembly mechanism of the multidrug efflux pump and the development of the screening system for pump inhibitorsPrincipal Investigator

    • Principal Investigator
      YOSHIHARA Eisaku
    • Project Period (FY)
      2002 – 2003
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      Bacteriology (including Mycology)
    • Research Institution
      Tokai University
  •  Structure and function of the xenobiotic-antibiotic extrusion transporters

    • Principal Investigator
      NAKAE Taiji
    • Project Period (FY)
      2001 – 2005
    • Research Category
      Grant-in-Aid for Scientific Research on Priority Areas
    • Review Section
      Biological Sciences
    • Research Institution
      Tokai University
  •  Structure and function of the xenobiotic-multidrug efflux pump

    • Principal Investigator
      NAKAE Taiji
    • Project Period (FY)
      2000 – 2002
    • Research Category
      Grant-in-Aid for Scientific Research (B)
    • Research Field
      Bacteriology (including Mycology)
    • Research Institution
      Tokai University
  •  The outer membrane components of xenobiotic efflux pumps are discovered to be members of a novel channel family with the unique structurePrincipal Investigator

    • Principal Investigator
      YOSHIHARA Eisaku
    • Project Period (FY)
      1999 – 2000
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      Bacteriology (including Mycology)
    • Research Institution
      Tokai University
  •  Structure and Function of the Antibiotic-Specific Channel

    • Principal Investigator
      NAKAE Taiji
    • Project Period (FY)
      1998 – 1999
    • Research Category
      Grant-in-Aid for Scientific Research (B).
    • Research Field
      Structural biochemistry
    • Research Institution
      Tokai University
  •  The xenobiotic efflux pumps and multiantibiotic resistance in Pseudomonas aeruginosa

    • Principal Investigator
      NAKAE Taiji
    • Project Period (FY)
      1997 – 1999
    • Research Category
      Grant-in-Aid for Scientific Research (B)
    • Research Field
      Bacteriology (including Mycology)
    • Research Institution
      Tokai University
  •  Elucidation of the molecular structure of OprD porin bearing the protease activity and the discovery of theporin homologous with OprDPrincipal Investigator

    • Principal Investigator
      YOSHIHARA Eisaku
    • Project Period (FY)
      1997 – 1998
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      Bacteriology (including Mycology)
    • Research Institution
      Tokai University
  •  Porin bearing the protease activity : its structure and functionPrincipal Investigator

    • Principal Investigator
      YOSHIHARA Eisaku
    • Project Period (FY)
      1995 – 1996
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      Bacteriology (including Mycology)
    • Research Institution
      Tokai University
  •  The permeability specificity of the imipenem-specific channel of OprD2 in Pseudomonas aeruginosa

    • Principal Investigator
      NAKAE Taiji
    • Project Period (FY)
      1993 – 1994
    • Research Category
      Grant-in-Aid for General Scientific Research (B)
    • Research Field
      Bacteriology (including Mycology)
    • Research Institution
      Tokai University School of Medicine
  •  Molecular and structural mechanism of the gating of the porin channelPrincipal Investigator

    • Principal Investigator
      YOSHIHARA Eisaku
    • Project Period (FY)
      1993 – 1994
    • Research Category
      Grant-in-Aid for General Scientific Research (C)
    • Research Field
      Bacteriology (including Mycology)
    • Research Institution
      Tokai University
  •  ポーリンチャンネル開閉の分子機構Principal Investigator

    • Principal Investigator
      良原 栄策
    • Project Period (FY)
      1992
    • Research Category
      Grant-in-Aid for General Scientific Research (C)
    • Research Field
      細菌学
    • Research Institution
      Tokai University
  •  緑膿菌ポ-リン蛋白質のドメイン構造とその機能Principal Investigator

    • Principal Investigator
      良原 栄策
    • Project Period (FY)
      1991
    • Research Category
      Grant-in-Aid for General Scientific Research (C)
    • Research Institution
      Tokai University
  •  緑膿菌ポ-リン蛋白質の分子集合と機能発現Principal Investigator

    • Principal Investigator
      良原 栄策
    • Project Period (FY)
      1990
    • Research Category
      Grant-in-Aid for General Scientific Research (C)
    • Research Field
      細菌学
    • Research Institution
      Tokai University
  •  Role of the outer membrane permeability in the antibiotic resistance in Pseudomonas aerugionsa

    • Principal Investigator
      NAKAE Taiji
    • Project Period (FY)
      1990 – 1991
    • Research Category
      Grant-in-Aid for General Scientific Research (B)
    • Research Field
      細菌学
    • Research Institution
      Tokai University
  •  新しく同定した緑膿菌外膜のポーリン群の構造、物質透過機序及びその生物学的意義Principal Investigator

    • Principal Investigator
      良原 栄策
    • Project Period (FY)
      1988
    • Research Category
      Grant-in-Aid for General Scientific Research (C)
    • Research Field
      細菌学
    • Research Institution
      Tokai University
  •  Role of the outer membrane of gram-negativebacteria in the intrinsic drug resistance

    • Principal Investigator
      NAKAE Taiji
    • Project Period (FY)
      1987 – 1988
    • Research Category
      Grant-in-Aid for General Scientific Research (C)
    • Research Field
      細菌学
    • Research Institution
      Tokai University School of Medicine
  •  緑膿菌外膜の透過孔と抗生物質透過性の関連

    • Principal Investigator
      NAKAE Taiji
    • Project Period (FY)
      1986
    • Research Category
      Grant-in-Aid for General Scientific Research (C)
    • Research Field
      細菌学
    • Research Institution
      Tokai University

All 2009 2008 2007 2006 2005 2004 2003 2002

All Journal Article Presentation Patent

  • [Journal Article] Modification of a human monoclonal antibody Fab fragment specific for Plasmodium falciparum 19-kDa C-terminal merozoite surface protein 1 by site-directed mutagenesis2008

    • Author(s)
      Tao,Y.-L., Chen, X.-J., Fu, Y.-F., Tsukamoto, H., Yoshihara, E.Tachibana, H.
    • Journal Title

      Parasitol. Res. 103

      Pages: 429-433

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19590458
  • [Journal Article] Diversity in the oligomeric channel structure of the multidrug efflux pumps in Pseudomonas aeruginosa2007

    • Author(s)
      Yoshihara, E.
    • Journal Title

      Microbiol. Immunol. 51・1

      Pages: 47-52

    • NAID

      10020281830

    • Description
      「研究成果報告書概要(和文)」より
    • Data Source
      KAKENHI-PROJECT-17590402
  • [Journal Article] Diversity in the oligomeric channel structure of the multidrug efflux pumps in Pseudomonas aeruginosa2007

    • Author(s)
      Yoshihara, E., Eda, S.
    • Journal Title

      Microbiol.Immunol. 51(1)

      Pages: 47-52

    • NAID

      10020281830

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-17590402
  • [Journal Article] Diversity in the oligomeric channel structure of the multidrug efflux pumps in Pseudomonas aeruginosa2007

    • Author(s)
      Yoshihara, E., Eda, S.
    • Journal Title

      Microbiol. Immunol. 51

      Pages: 47-52

    • NAID

      10020281830

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19590458
  • [Journal Article] Diversity in the oligomeric channel structure of the multidrug efflux pumps in Pseudomonas aeruginosa2007

    • Author(s)
      Yoshihara, E.
    • Journal Title

      Microbiol.Immunol. 51.1

      Pages: 47-52

    • NAID

      10020281830

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19590458
  • [Journal Article] Assignment of the outer-membrane-subunit-selective domain of the membrane fusion protein in the tripartite xenobiotic efflux pump of Pseudomonas aeruginosa2006

    • Author(s)
      Eda, S., Maseda, H., Yoshihara, E., Nakae T.
    • Journal Title

      FEMS Microbiol. Letts. 254

      Pages: 101-107

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-13142209
  • [Journal Article] Assignment of the outer-membrane-subunit- selective domain of the membrane fusion protein in the tripartite xenobiotic efflux pump of Pseudomonas aeruginosa2006

    • Author(s)
      Eda, S., Maseda, H., Yoshihara, E., Nalae, T.
    • Journal Title

      FEMS Microbiol.Lett. 254

      Pages: 101-107

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-17590402
  • [Journal Article] Multidrug transporter MexB of Pseudomonas aeruginosa : overexpression, purification, and initial structural characterization 20062005

    • Author(s)
      Mokhonov, v., Mokhonova, E., Yoshihara,....Akama, H., Nakae, T.
    • Journal Title

      Protein Expression and Purification 40

      Pages: 91-100

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-13142209
  • [Journal Article] Multidrug transporter MexB of Pseudomonas aeruginosa : overexpression, purification, and initial structural characterization2005

    • Author(s)
      Mokohonov, V., Mokohonova, E, Yoshihara, E., Masui, R., Sakai, M., Akama, H., Nakae, T.
    • Journal Title

      Protein Expr.Purif. 40

      Pages: 91-100

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-17590402
  • [Journal Article] MexZ-mediated regulation of mexXY multidrug efflux pumpexpression in Pseudomonas aeruginosa by binding on the mexZ-MexX intergenic DNA2004

    • Author(s)
      Matsuo, Y., Eda, S., Gotoh, N., Yoshihara, E., Nakae, T.
    • Journal Title

      FEMS Microbiol. Lett. 238

      Pages: 23-28

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-13142209
  • [Journal Article] Linkage of the efflux-pump expression level with substrate extrusion rate in the MexAB-OprM efflux pump of Pseudomonas aeruginosa2003

    • Author(s)
      Narita, S., Eda, S., Yoshihara, E., Nakae, T.
    • Journal Title

      Biochem. Biophys. Res. Commun. 308

      Pages: 922-926

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-13142209
  • [Journal Article] Mutations affecting DNA-binding activity of the MexR repressor of mexR-mexA-mexB-oprM operon expression 20042003

    • Author(s)
      Saito, K., Akama, H., Yoshihara, E., Nakae, T.
    • Journal Title

      J. Bacterio1. 183

      Pages: 6195-6198

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-13142209
  • [Journal Article] The outer membrane component of the multiddrug efflux poump from Pseudomonas aeruginosa may be a gated channel. 20032002

    • Author(s)
      Yoshihara, E., Maseda, H., Saito, K.
    • Journal Title

      Eur. J. Biochem. 269

      Pages: 4738-4745

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-13142209
  • [Journal Article] The outer membrane component of the multiddrug efflux poump from Pseudomonas aeruginosa may be a gated channel.2002

    • Author(s)
      Yoshihara, E.
    • Journal Title

      Eur.J.Biochem. 269

      Pages: 4738-4745

    • Description
      「研究成果報告書概要(和文)」より
    • Data Source
      KAKENHI-PROJECT-13142209
  • [Journal Article] A novel assembly process of the multicomponent xenobiotic efflux pump in Pseudomonas aeruginosa2002

    • Author(s)
      Maseda, H., Kitao, M., Eda, S., Yoshihara, E, Nakae, T.
    • Journal Title

      Molec. Microbiol. 46

      Pages: 677-686

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-13142209
  • [Patent]2009

    • Inventor(s)
      猪子英俊、良原栄策、光永滋樹
    • Industrial Property Rights Holder
      東海大学
    • Filing Date
      2009-06-16
    • Data Source
      KAKENHI-PROJECT-19101008
  • [Patent] 緑膿菌の薬剤排出ポンプの機能を阻害する方法及び薬剤2006

    • Inventor(s)
      良原 栄策, 猪子 英俊
    • Industrial Property Rights Holder
      学校法人東海大学
    • Industrial Property Number
      2006-033522
    • Filing Date
      2006-02-10
    • Data Source
      KAKENHI-PROJECT-17590402
  • [Patent] 緑膿菌の薬剤排出ポンプの機能を阻害する方法及び薬剤2006

    • Inventor(s)
      良原栄策, 猪子英俊
    • Industrial Property Rights Holder
      学校法人東海大学
    • Industrial Property Number
      2006-033522
    • Filing Date
      2006-02-10
    • Description
      「研究成果報告書概要(和文)」より
    • Data Source
      KAKENHI-PROJECT-17590402
  • [Presentation] Novel peptidyl inhibitor of MexAB-OprM, a multidrug efflux pump in Pseudomonas aeruginosa2009

    • Author(s)
      良原 栄策
    • Organizer
      第82回日本細菌学会総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2009-03-12
    • Data Source
      KAKENHI-PROJECT-19590458
  • [Presentation] Novel peptidyl inhibitor of MexAB-OprM, a multidrug efflux pump in Pseudomonas aeruginosa2009

    • Author(s)
      良原 栄策
    • Organizer
      日本細菌学会総会
    • Place of Presentation
      名古屋国際会議場
    • Data Source
      KAKENHI-PROJECT-19590458
  • [Presentation] 緑膿菌多剤排出ポンプMexAB-OprMの外膜チャネルであるOprMが関与する基質認識2008

    • Author(s)
      良原 栄策
    • Organizer
      第80回日本細菌学会総会
    • Place of Presentation
      大阪アジア太平洋トレードセンター
    • Year and Date
      2008-03-27
    • Data Source
      KAKENHI-PROJECT-19590458
  • [Presentation] 緑膿菌多剤排出ポンプMexAB-OprMの外膜チャネルであるOprMが関与する基質認識2007

    • Author(s)
      良原 栄策
    • Organizer
      日本細菌学会総会
    • Place of Presentation
      アジア太平洋トレードセンタ
    • Data Source
      KAKENHI-PROJECT-19590458
  • 1.  NAKAE Taiji (50102851)
    # of Collaborated Projects: 8 results
    # of Collaborated Products: 6 results
  • 2.  YONEYAMA Hiroshi (10220774)
    # of Collaborated Projects: 6 results
    # of Collaborated Products: 0 results
  • 3.  ISHII Junko (20212813)
    # of Collaborated Projects: 2 results
    # of Collaborated Products: 0 results
  • 4.  NARITA Shin-ichiro (30338751)
    # of Collaborated Projects: 2 results
    # of Collaborated Products: 1 results
  • 5.  KOBAYASHI Yoriko
    # of Collaborated Projects: 2 results
    # of Collaborated Products: 0 results
  • 6.  INOKO Hidetoshi (10101932)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 7.  OKA Akira (80384866)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 8.  ENDOH Takaho (40384862)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 9.  OHTSUKA Masato (90372945)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 10.  HIRAYAMA Noriaki (70238393)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 11.  TANI Ayako (70284867)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 12.  SATAKE Sachiko
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 13.  GABRIEL Rumm
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 14.  TILMAN Schir
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 15.  JURG P Rosen
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results

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