KOBAYASHI Masayuki 小林正幸

Researcher Number	70271864
Other IDs
Affiliation (Current)	2025: 有明工業高等専門学校, 創造工学科, 教授
Affiliation (based on the past Project Information) *help	2007 – 2009: 有明工業高等専門学校, 物質工学科, 准教授 2006: 有明工業高等専門学校, 物質工学科, 助教授 2005: 有明高等専門学校, 物質工学科, 助教授 1997 – 2004: TOHOKU UNIVERSITY,GRADUATE SCHOOL OF ENGINEERING,DEPARTMENT OF BIOCHEMISTRY AND, 大学院・工学研究科, 助手 1998: 東北大学, 大学院工学研究科, 助手 1995 – 1996: TOHOKU UNIVERSITY,FACULTY OF ENGINEERING,RESEARCHASSOCIATE, 工学部, 助手
Review Section/Research Field	Principal Investigator 生物・生体工学 / Biophysics Except Principal Investigator 工業物理化学 / 生物・生体工学 / Chemistry related to living body / Biological Sciences / Structural biochemistry
Keywords	Principal Investigator アルギニン残基 / 磁気円偏光二色性 / 光合成反応中心 / 変異株 / 熱安定化 / 常温菌 / アルギニン / 膜界面 / 静電的相互作用 / 戦略的変異導入 … More / X線結晶造解析 / 膜タンパク質 / 耐熱菌 / 配位状態 / LH1αサブユニット / バクテリオクロロフィル / 光合成膜内アンテナ / Zn-BChl / 電子的相互作用 / 電子構造 / 配位構造 / 色素会合体 / D解析 / B / 膜内アンテナ / PCR法 / 1次構造 / 水素結合 / Resonance Raman / 二量体 / スペシャルペア … More Except Principal Investigator クロロゾームアンテナ / 固体高分解能NMR / MCD / 光合成 / バクテリオクロロフィル / B820 / BCh1 c / BCh1 a / 膜タンパク質 / Solid state high resolution NMR / RFDR / 光合成膜 / 色素 / 核磁気共鳴 / 光捕集複合体 / 光合成色素 / ^<31>PNMR / クロロゾーム / 光合成細菌 / Chlorosome antenna / LH1(13870) / Photosynthetic antenna complex / Solution NMR / 多次元NMR / 配位構造 / 光捕集系錯体1 / アンテナタンパク質 / 構造解析 / BChlc / BChla / LH1 / LH1(870) / 光合成アンテナタンパク質 / 溶液NMR / bacteriochlorophyll / nuclear magnetic resonance / light-harvesting complex / membrane protein / photosynthetic pigment / Isotope label / Photosynthetic antenna / Dimer / Bacteriochlorophyll c / Chlorosome / タイマー / 安定同位体標識 / 光合成アンテナ / ダイマー / バクテリオクロロフィルc / 固体高分解NMR / ENERGY CONVERSION / BACTERIUM / PHOTOSYNTHETIC / AGGREGATE / LIGHT-HARVESTING COMPLEX / PIGMENT / SMALL-ANGLE SCATTERING / PHOTOSYNTHESIS / 光補集器官 / エネルギー変換 / 会合体 / 光捕集器官 / 小角散乱 / P-31 NMR / ATP SYNTHESIS / X-RAY DIFFRACTION SPECTROSCOPY / SOLID STATE NMR / CHLOROSOME ANTENNA / MEMBRANE ANTENNA / REACTION CENTER COMPLEX / PHOTOSYNTHETIC MEMBRANE / Rubisco / ^<13>CNMR / 光合成膜顆粒 / 光ATP合成活性 / エネルギー産生生体膜 / 光合成反応 / ^<31>P NMR / ATP合成反応 / X線分光法 / 膜内アンテナ / 半生体人工光合成 / 励起エネルギー移動 / ATP合成酵素 / 反応中心錯体 / アンテナ錯体 / CYTOCHROME / BACTERIOCHLOROPHYLL / MULTIDIMENSIONAL SPECTRA / THIN OPTICAL CELL / MAGNETIC CIRCULAR DICHROISM / CIRCULAR DICHROISM / LINEAR DICHROISM / SUPERMOLECULAR STRUCTURE / アンテナ色素 / 生体機能構造 / 光合成の反応中心 / 二次元スペクトル / CD / 薄膜光学セル / シトクロム / 多次元解析 / 薄層光学セル / 多次元スペクトル / 直線偏光二色性 / 磁気円偏光二色性 / 円偏光二色性 / 超分子構造 / LH2 / 沈殿剤 / デタージェント / 超分子膜タンパク質 / PufX / LH1 Qy 遷移 / LHI Qy遷移 / LH1 Qy遷移 / X線構造解析 / Thermochromatium tepidum / LH1-RC複合体 / 結晶化 / 膜タンパク質超分子複合体 / 光電変換 / 色素膜タンパク質 / 光捕集反応中心複合体 / バクテリクロロフィル / 光捕集系 / 膜蛋白質 / 酵素活性 / オキシゲナーゼ / カルボキシラーゼ / NMR / 炭酸固定 / ルビスコ / CO_2固定反応 / 膜顆粒 / 反応中心 / アンテナ / 電気化学反応 Less

Molecular mechanism of the functional regulation of the bacterial photosynthetic light-harvesting-reaction center complexes
- Principal Investigator
  
  OTOMO Seiu, 野澤庸則
- Project Period (FY)
  2004 – 2009
- Research Category
  
  Grant-in-Aid for Scientific Research on Priority Areas
- Review Section
  
  Biological Sciences
- Research Institution
  Ibaraki University
  Tohoku University
Structures and Functions of Membrane Antenna Proteins with High Resolution Solution and Solid State NMR
- Principal Investigator
  
  NOZAWA Tsunenori
- Project Period (FY)
  2003 – 2004
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  Chemistry related to living body
- Research Institution
  Tohoku University
戦略的変異導入による耐熱性向上膜タンパク質の創製Principal Investigator
- Principal Investigator
  
  小林正幸
- Project Period (FY)
  2002 – 2003
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  生物・生体工学
- Research Institution
  Tohoku University
Supramolecular structural analysis of photosynthetic light-harvesting machinery
- Principal Investigator
  
  OTOMO Seiu
- Project Period (FY)
  2002 – 2004
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  工業物理化学
- Research Institution
  Tohoku University
溶液核磁気共鳴法による光合成色素膜蛋白質複合体の構造と機能解析
- Principal Investigator
  
  大友征宇
- Project Period (FY)
  2000 – 2001
- Research Category
  
  Grant-in-Aid for Exploratory Research
- Research Field
  
  Structural biochemistry
- Research Institution
  Tohoku University
Structural Analysis of Intact Biomolecules by Solid State High Resolution Nuclear Magnetic Resonance with Homogenously Isotope Labeled Samples
- Principal Investigator
  
  NOZAWA Tsunenori
- Project Period (FY)
  2000 – 2001
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  工業物理化学
- Research Institution
  Tohoku University
光合成膜内アンテナの微細会合構造解析とアンテナ機能発現のための色素会合要因の決定Principal Investigator
- Principal Investigator
  
  小林正幸
- Project Period (FY)
  1998 – 1999
- Research Category
  
  Grant-in-Aid for Encouragement of Young Scientists (A)
- Research Field
  
  Biophysics
- Research Institution
  Tohoku University
核磁気共鳴法による生体系CO_2固定反応追跡手法の開発
- Principal Investigator
  
  王征宇
- Project Period (FY)
  1997 – 1998
- Research Category
  
  Grant-in-Aid for Exploratory Research
- Research Field
  
  生物・生体工学
- Research Institution
  Tohoku University
光合成反応中心の器の構造解析とその工学的応用Principal Investigator
- Principal Investigator
  
  小林正幸
- Project Period (FY)
  1996
- Research Category
  
  Grant-in-Aid for Encouragement of Young Scientists (A)
- Research Field
  
  生物・生体工学
- Research Institution
  Tohoku University
STRUCTURAL AND FUNCTIONAL ANALYSES OF PHOTOSYNITHETIC ANTENNA COMPLEXES BY SCATTERING METHODS
- Principal Investigator
  
  WANG Zheng-Yu
- Project Period (FY)
  1996 – 1997
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  生物・生体工学
- Research Institution
  TOHOKU UNIVERSITY
光合成機能に電気化学反応を組合せたNADHの再生と二酸化炭素の固定反応
- Principal Investigator
  
  NOZAWA Tsunenori
- Project Period (FY)
  1995
- Research Category
  
  Grant-in-Aid for Scientific Research on Priority Areas
- Research Institution
  Tohoku University
MECHANISM AND STRUCTURE OF PHOTOSYNTHETIC REACTION AND APPRATUS FOR APPLICATION OF ITS MECHANISM TO ENERGY PRODUCING BIOMEMBRANE
- Principal Investigator
  
  NOZAWA Tsunenori
- Project Period (FY)
  1995 – 1996
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  生物・生体工学
- Research Institution
  TOHOKU UNIVERSITY
DEVELOPMENT OF 2D LINEAR AND CIRCULAR DICHROMETER AND ITS APPLLICATION FOR BIOLOGICAL MOLECULAR SYSTEMS
- Principal Investigator
  
  NOZAWA Tsunenori
- Project Period (FY)
  1994 – 1995
- Research Category
  
  Grant-in-Aid for Developmental Scientific Research (B)
- Research Field
  
  工業物理化学
- Research Institution
  TOHOKU UNIVERSITY

All 2009 2008 2007 2005 2004

All Journal Article Presentation

[Journal Article] Calcium ions are involved in the unusual red-shift of the light-harvesting 1 Qy transition of the core complex in thermophilic purple sulfur bacterium Thermochromatium tepidum.2008
- Author(s)
  Kimura, Y., Hirano, Y., Yu, L. -J., Suzuki, H., Kobayashi, M., Wang, Z. -Y.
- Journal Title
  
  J. Biol. Chem. 283
  
  Pages: 13867-13873
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16087201
[Journal Article] Overexpression and characterization of the Rhodobacter sphaeroides PufX membrane protein in Escherichia coli.2007
- Author(s)
  Onodera, S., Suzuki, H., Shimada, Y., Kobayashi, M., Nozawa, T., Wang, Z. -Y.
- Journal Title
  
  Photochem. Photobiol. 83
  
  Pages: 139-144
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16087201
[Journal Article] Solution structure of the Rhodobacter sphaeroides PufX membrane protein: Implications for the quinone exchange and protein-protein interactions.2007
- Author(s)
  Wang, Z. -Y., Suzuki, H., Kobayashi, M., Nozawa, T.
- Journal Title
  
  Biochemistry 46
  
  Pages: 3635-3642
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16087201
[Journal Article] Purification, characterization and crystallization of the core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum.2007
- Author(s)
  Suzuki, H., Hirano, Y., Kimura, Y., Tataich, S., Kobayashi, M., Miki, K., Wang, Z. -Y.
- Journal Title
  
  Biochim. Biophys. Acta 1767
  
  Pages: 1057-1063
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16087201
[Journal Article] Isotopic labeling of proteins by utilizing photosynthetic bacteria.2005
- Author(s)
  Suzuki, H., Shimada, Y., Kobayashi, M., Kudo, M., Nozawa, T., Wang, Z. -Y.
- Journal Title
  
  Anal. Biochem. 347
  
  Pages: 324-326
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16087201
[Journal Article] Reconstitution of photosynthetic reaction centers and core antenna-reaction center complexes in liposomes and their thermal stability.2005
- Author(s)
  Kobayashi, M., Fujioka, Y., Mori, T., Terashima, M., Suzuki, H., Shimada, Y., Saito, T., Wang, Z. -Y., Nozawa, T.
- Journal Title
  
  Biosci. Biotechnol. Biovhem. 69
  
  Pages: 1130-1136
- NAID
  130000030298
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16087201
[Journal Article] Electronic properties and thermal stability of soluble redox proteins from a thermophilic sulfur photosynthetic bacterium, Thermochromatium tepidum2005
- Author(s)
  Kobayashi, et al.
- Journal Title
  
  Bull.Chem.Soc.Jpn. 78
  
  Pages: 2164-2170
- Data Source
  KAKENHI-PROJECT-16087201
[Journal Article] Reconstitution of photosynthetic reaction centers and core antenna-reaction center complexes in liposomes and their thermal stability2005
- Author(s)
  Kobayashi, et al.
- Journal Title
  
  Biosci.Biotechnol.Biochem. 69
  
  Pages: 1130-1136
- NAID
  130000030298
- Data Source
  KAKENHI-PROJECT-16087201
[Journal Article] Solution structures of the core light-harvesting a and b polypeptides from Rhodospirillum rubrum: Implications for the pigment-protein and protein-protein interactions.2005
- Author(s)
  Wang, Z. -Y., Gokan., Kobayashi, M., Nozawa, T.
- Journal Title
  
  J. Mol. Biol. 347
  
  Pages: 465-477
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16087201
[Journal Article] Electronic properties and thermal stability of soluble redox proteins from a thermophilic purple sulfur photosynthetic bacterium, Thermochromatium tepidum.2005
- Author(s)
  Kobayashi, M., Saito, T., Takahashi, K., Wang, Z. -Y., Nozawa, T.
- Journal Title
  
  Bull. Chem. Soc. Jpn. 78
  
  Pages: 2164-2170
- NAID
  10017074556
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16087201
[Journal Article] Reconstitution and replacement of bacteriochlorophyll a molecules in photosynthetic reaction center2004
- Author(s)
  Masayuki Kobayashi et al.
- Journal Title
  
  J.Biochemistry 136
  
  Pages: 363-369
- NAID
  10016202243
- Data Source
  KAKENHI-PROJECT-16087201
[Journal Article] Reconstitution and replacement of bacteriochlorophyll a molecules in photosynthetic reaction centers2004
- Author(s)
  Kobayashi, M., et al.
- Journal Title
  
  J.Biochem. 136
  
  Pages: 363-369
- NAID
  10016202243
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-14550784
[Journal Article] Reconstitution and replacement of bacteriochlorophyll a molecules in photosynthetic reaction centers2004
- Author(s)
  M.Kobayashi, et al
- Journal Title
  
  J.Biochem. 136
  
  Pages: 363-369
- NAID
  10016202243
- Data Source
  KAKENHI-PROJECT-14550784
[Journal Article] Reconstitution and replacement of bacteriochlorophyll α molecules in photosynthetic reaction centers2004
- Author(s)
  Kobayashi, M., et al.
- Journal Title
  
  J.Biochem. 136
  
  Pages: 363-369
- NAID
  130003534731
- Description
  「研究成果報告書概要(和文)」より
- Data Source
  KAKENHI-PROJECT-14550784
[Presentation] 好熱性光合成細菌由来の光捕集光電変換タンパク質の単離精製と結晶化2009
- Author(s)
  小林美穂、清水佑記、堀口健太郎、大畠史一、干龍江、平野優、大友征宇
- Organizer
  茨城地区交流会
- Place of Presentation
  日立
- Year and Date
  2009-11-06
- Data Source
  KAKENHI-PROJECT-16087201

1. OTOMO Seiu (10213612)

# of Collaborated Projects: 11 results

# of Collaborated Products: 9 results
2. NOZAWA Tsunenori (10006322)

# of Collaborated Projects: 10 results

# of Collaborated Products: 6 results
3. SHIKAMA Yuko (80250710)

# of Collaborated Projects: 2 results

# of Collaborated Products: 0 results
4. SAKAYANAGI Nobuyuki

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results

KOBAYASHI Masayuki 小林 正幸

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戦略的変異導入による耐熱性向上膜タンパク質の創製Principal Investigator

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光合成膜内アンテナの微細会合構造解析とアンテナ機能発現のための色素会合要因の決定Principal Investigator

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光合成反応中心の器の構造解析とその工学的応用Principal Investigator

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[Journal Article] Calcium ions are involved in the unusual red-shift of the light-harvesting 1 Qy transition of the core complex in thermophilic purple sulfur bacterium Thermochromatium tepidum.2008

Author(s)

Journal Title

Data Source

[Journal Article] Overexpression and characterization of the Rhodobacter sphaeroides PufX membrane protein in Escherichia coli.2007

Author(s)

Journal Title

Data Source

[Journal Article] Solution structure of the Rhodobacter sphaeroides PufX membrane protein: Implications for the quinone exchange and protein-protein interactions.2007

KOBAYASHI Masayuki 小林正幸