Takahashi Daisuke 高橋大輔

Researcher Number	70791523
Other IDs
Affiliation (Current)	2025: 崇城大学, 薬学部, 准教授
Affiliation (based on the past Project Information) *help	2023: 崇城大学, 薬学部, 准教授 2020 – 2022: 九州大学, 薬学研究院, 講師 2018 – 2020: 九州大学, 薬学研究院, 助教 2017: 千葉大学, 大学院理学研究院, 特任助教
Review Section/Research Field	Principal Investigator Basic Section 43020:Structural biochemistry-related / Biological pharmacy Except Principal Investigator Basic Section 43040:Biophysics-related
Keywords	Principal Investigator 脂質代謝酵素 / DGK / 構造解析 / ジアシルグリセロールキナーゼ / DGKα / EFハンド / 脂質キナーゼ / クライオ電顕単粒子解析 / 単粒子解析 / クライオ電顕 … More / 分子間相互作用 / 構造生物学 / 複数ドメイン蛋白質 / EF-hand / マルチドメイン酵素 / 溶液NMR / SEC-SAXS/MALS / X線小角散乱 / NMR / 相互作用ネットワーク / シグナル脂質 / 細胞内情報伝達 / シグナル伝達 / がん免疫 / EFハンドモチーフ / 立体構造解析 / 昆虫細胞・バキュロウイルス / マルチドメイン蛋白質 … More Except Principal Investigator mechanism / molecular interactions / Molecular interactios / Mechanism / protein expression / Antobody / fc receptor / antibody / HIV-1 / bnAbs / glycosylation / immunology / structure / hinge / Fc receptor / Antibody Less

Structural basis of diacylglycerol kinase alpha, a novel target for cancer immunotherapyPrincipal Investigator
- Principal Investigator
  
  高橋大輔
- Project Period (FY)
  2023 – 2025
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 43020:Structural biochemistry-related
- Research Institution
  Sojo University
Understanding the efficacy of therapeutic antibodies through their interaction with cellular receptors.
- Principal Investigator
  
  CAAVEIRO Jose
- Project Period (FY)
  2020 – 2022
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 43040:Biophysics-related
- Research Institution
  Kyushu University
Structural basis for enzymatic catalysis and regulation of diacylglycerol kinase alphaPrincipal Investigator
- Principal Investigator
  
  Takahashi Daisuke
- Project Period (FY)
  2019 – 2021
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 43020:Structural biochemistry-related
- Research Institution
  Kyushu University
Molecular basis underlying cancer cell proliferation and T-cell anergy induction by diacylglycerol kinase alphaPrincipal Investigator
- Principal Investigator
  
  Takahashi Daisuke
- Project Period (FY)
  2017 – 2018
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Biological pharmacy
- Research Institution
  Kyushu University
  Chiba University

All 2021 2020 2019 2018 2017

All Journal Article Presentation

[Journal Article] Membrane morphology determines diacylglycerol kinase alpha substrate acyl chain specificit2021
- Author(s)
  Jose Carlos Bozelli Junior, Jenny Yune, Daisuke Takahashi, Fumio Sakane, and Richard M. Epand
- Journal Title
  
  FASEB Journal
  
  Volume: Accepted
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K06527
[Journal Article] Membrane morphology determines diacylglycerol kinase alpha substrate acyl chain specificity.2021
- Author(s)
  Bozelli Jr., J.C., Jenny Yune, J., Takahashi, D., Sakane, F., and Epand, R.M.
- Journal Title
  
  FASEB Journal
  
  Volume: 35 Issue: 6
- DOI
  10.1096/fj.202100264r
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K06527, KAKENHI-PROJECT-20H03205
[Journal Article] Palmitic acid- and/or palmitoleic acid-containing phosphatidic acids are generated by diacylglycerol kinase α in starved Jurkat T cells2020
- Author(s)
  Murakami Y, Murakami C, Hoshino F, Lu Q, Akiyama R, Yamaki A, Takahashi D, Sakane F.
- Journal Title
  
  Biochemical and Biophysical Research Communications
  
  Volume: in press Issue: 4 Pages: 1054-1060
- DOI
  10.1016/j.bbrc.2020.02.162
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-19K06527, KAKENHI-PROJECT-17H03650, KAKENHI-PROJECT-18J20003, KAKENHI-PROJECT-20J21133, KAKENHI-PROJECT-20H03205
[Journal Article] Crystal structure and calcium-induced conformational changes of diacylglycerol kinase α EF-hand domains2019
- Author(s)
  Takahashi Daisuke、Suzuki Kano、Sakamoto Taiichi、Iwamoto Takeo、Murata Takeshi、Sakane Fumio
- Journal Title
  
  Protein Science
  
  Volume: 28 Issue: 4 Pages: 694-706
- DOI
  10.1002/pro.3572
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18K11536, KAKENHI-PROJECT-17H03650, KAKENHI-PROJECT-17K15444, KAKENHI-PROJECT-19K06527
[Journal Article] Characterization of α-synuclein N-terminal domain as a novel cellular phosphatidic acid sensor2019
- Author(s)
  Yamada H, Mizuno S, Honda S, Takahashi D, Sakane F
- Journal Title
  
  FEBS J
  
  Volume: in press Issue: 11 Pages: 2212-2234
- DOI
  10.1111/febs.15137
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19K06527, KAKENHI-PROJECT-17H03650
[Journal Article] Myristic acid specifically stabilizes diacylglycerol kinase delta protein in C2C12 skeletal muscle cells.2019
- Author(s)
  Iwata, K., Sakai, H., Takahashi, D. and Sakane F.
- Journal Title
  
  Biochim Biophys Acta - Mol Cell Biol Lipids
  
  Volume: In press Issue: 7 Pages: 1031-1038
- DOI
  10.1016/j.bbalip.2019.04.003
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K15444, KAKENHI-PROJECT-19K06527, KAKENHI-PROJECT-17H03650, KAKENHI-PROJECT-17K08271
[Journal Article] Microarray analysis of gene expression in the diacylglycerol kinase η knockout mouse brain2019
- Author(s)
  Komenoi S, Suzuki Y, Asami M, Murakami C, Hoshino F, Chiba S, Takahashi D, Kado S, Sakane F.
- Journal Title
  
  Biochemistry and Biophysics Reports
  
  Volume: 19 Pages: 100660-100660
- DOI
  10.1016/j.bbrep.2019.100660
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-19K06527, KAKENHI-PROJECT-17H03650, KAKENHI-PROJECT-18J20003
[Journal Article] Expression, purification and characterization of human diacylglycerol kinase zeta2019
- Author(s)
  Saito, T., Takahashi, D. and Sakane, F.
- Journal Title
  
  ACS Omega
  
  Volume: 4 Issue: 3 Pages: 5540-5546
- DOI
  10.1021/acsomega.9b00079
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-17K15444, KAKENHI-PROJECT-19K06527
[Journal Article] Diacylglycerol kinase β induces filopodium formation via its C1, catalytic and carboxy-terminal domains and interacts with the Rac1-GTPase-activating protein, β2-chimaerin2018
- Author(s)
  Maeda Yuki、Shibata Kosuke、Akiyama Rino、Murakami Yuki、Takao Saki、Murakami Chiaki、Takahashi Daisuke、Sakai Hiromichi、Sakane Fumio
- Journal Title
  
  Biochemical and Biophysical Research Communications
  
  Volume: 504 Issue: 1 Pages: 54-60
- DOI
  10.1016/j.bbrc.2018.08.119
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K08271, KAKENHI-PROJECT-17H03650, KAKENHI-PROJECT-18J20003, KAKENHI-PROJECT-17K15444
[Journal Article] Where do substrates of diacylglycerol kinases come from? Diacylglycerol kinases utilize diacylglycerol species supplied from phosphatidylinositol turnover-independent pathways.2018
- Author(s)
  Sakane, F., Mizuno, S., Takahashi, D., Sakai, H.
- Journal Title
  
  Adv. Biol. Regul.
  
  Volume: 67 Pages: 101-108
- DOI
  10.1016/j.jbior.2017.09.003
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-17K08271, KAKENHI-PROJECT-17K15444, KAKENHI-PROJECT-17H03650
[Journal Article] Expression and purification of human diacylglycerol kinase α from baculovirus-infected insect cells for structural studies2018
- Author(s)
  Takahashi Daisuke、Sakane Fumio
- Journal Title
  
  PeerJ
  
  Volume: 6 Pages: e5449-e5449
- DOI
  10.7717/peerj.5449
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-17H03650, KAKENHI-PROJECT-17K15444
[Journal Article] Diacylglycerol kinase α‐selective inhibitors induce apoptosis and reduce viability of melanoma and several other cancer cell lines2018
- Author(s)
  Yamaki Atsumi、Akiyama Rino、Murakami Chiaki、Takao Saki、Murakami Yuki、Mizuno Satoru、Takahashi Daisuke、Kado Sayaka、Taketomi Akinobu、Shirai Yasuhito、Goto Kaoru、Sakane Fumio
- Journal Title
  
  Journal of Cellular Biochemistry
  
  Volume: 120 Issue: 6 Pages: 10043-10056
- DOI
  10.1002/jcb.28288
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17H02175, KAKENHI-PROJECT-17H03650, KAKENHI-PROJECT-18J20003, KAKENHI-PROJECT-17K15444, KAKENHI-PROJECT-17K11581
[Journal Article] Abnormalities of the serotonergic system in diacylglycerol kinase δ-deficient mouse brain.2018
- Author(s)
  Lu, Q., Komenoi, S., Usuki, T., Takahashi, D., and Sakane, F.
- Journal Title
  
  Biochemical and Biophysical Research Communications
  
  Volume: 497 Issue: 4 Pages: 1031-1037
- DOI
  10.1016/j.bbrc.2018.02.165
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K15444, KAKENHI-PROJECT-17H03650, KAKENHI-PROJECT-16J06865
[Presentation] マルチドメイン蛋白質DGKαの活性を制御する N末端領域の構造変化の解析2021
- Author(s)
  高橋大輔，米澤健人，沖崎悠希，嶋田睦，Jose Caarveiro，阿部義人，植田正，清水伸隆，坂根郁夫
- Organizer
  第94回日本生化学会大会
- Data Source
  KAKENHI-PROJECT-19K06527
[Presentation] DGKalpha活性制御の分子基盤：N末端RVH領域へのCa2+結合の物理化学的解析2019
- Author(s)
  高橋大輔，嶋田睦，Jose Caaveiro, 阿部義人，植田正，坂根郁夫
- Organizer
  日本生化学会九州支部例会
- Data Source
  KAKENHI-PROJECT-17K15444
[Presentation] DGKα 活性制御の分子基盤：N末端RVH領域へのCa2+結合の物理化学的解析2019
- Author(s)
  高橋大輔，嶋田睦，Jose Caarveiro, 阿部義人，植田正，坂根郁夫
- Organizer
  2019年度日本生化学会九州支部例会
- Data Source
  KAKENHI-PROJECT-19K06527
[Presentation] DGKα 活性制御の分子基盤：N末端RVH領域へのCa2+結合の物理化学的解析2019
- Author(s)
  高橋大輔，嶋田睦，Jose Caarveiro, 阿部義人，植田正，坂根郁夫
- Organizer
  第43回蛋白質と酵素の構造と機能に関する九州シンポジウム
- Data Source
  KAKENHI-PROJECT-19K06527
[Presentation] ジアシルグリセロールキナーゼδの欠損はセロトニン神経系の機能低下を惹起する2018
- Author(s)
  陸強, 米野井優, 高橋大輔, 坂根郁夫
- Organizer
  第60回日本脂質生化学会
- Data Source
  KAKENHI-PROJECT-17K15444
[Presentation] Abnormalities of the serotonergic system in diacylglycerol kinase δ-deficient mouse brains.2018
- Author(s)
  Lu, Q., Komenoi, S., Takahashi, D. and Sakane, F.
- Organizer
  59th International Conference on the Bioscience of Lipids "Lipid Fluxes and Metabolism; From Fundamental Mechanisms to Human Disease":
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K15444
[Presentation] Molecular basis for the intramolecular regulation of DGKα: analysis of Ca2+ induced conformational changes in N-terminal EF-hand motifs2018
- Author(s)
  Takahashi, D., Suzuki, K., Sakamoto, T., Iwamoto, T., Murata, T., and Sakane, F.
- Organizer
  第18回日本蛋白質科学会年会
- Data Source
  KAKENHI-PROJECT-17K15444
[Presentation] α-シヌクレインの脂質結合能の解析とPAプローブへの応用2018
- Author(s)
  山田遥夢, 高橋大輔, 坂根郁夫
- Organizer
  第91回日本生化学会大会
- Data Source
  KAKENHI-PROJECT-17K15444
[Presentation] Molecular basis for the intramolecular regulation of DGKα: analysis of Ca2+ induced conformational changes in N-terminal EF-hand motifs2018
- Author(s)
  Daisuke Takahashi, Kano Suzuki, Taiichi Sakamoto, Takeo Iwamoto, Takeshi Murata, and Fumio Sakane
- Organizer
  第18回　日本蛋白質科学会年会
- Data Source
  KAKENHI-PROJECT-17K15444
[Presentation] バキュロウイルス昆虫細胞発現系を用いたジアシルグリセロールキナーゼζの発現精製と酵素学的解析2018
- Author(s)
  西藤巧, 高橋大輔, 坂根郁夫
- Organizer
  第91回日本生化学会大会
- Data Source
  KAKENHI-PROJECT-17K15444
[Presentation] 新規リン脂質プローブの開発を目指したジアシルグリセロールキナーゼηプレクストリン相同ドメインの脂質結合解析2017
- Author(s)
  川瀬功暉，高橋大輔，坂本泰一，坂根郁夫
- Organizer
  日本分子生物学会/日本生化学会　2017年度生命科学系学会合同年次大会
- Data Source
  KAKENHI-PROJECT-17K15444

1. CAAVEIRO Jose (00536732)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
2. Sakane Fumio

# of Collaborated Projects: 1 results

# of Collaborated Products: 12 results
3. SAKAMOTO Taiichi

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
4. 堺弘道

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
5. 白井康仁

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results

Takahashi Daisuke 高橋 大輔

Research Projects

Research Products

Co-Researchers

Structural basis of diacylglycerol kinase alpha, a novel target for cancer immunotherapyPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Understanding the efficacy of therapeutic antibodies through their interaction with cellular receptors.

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Structural basis for enzymatic catalysis and regulation of diacylglycerol kinase alphaPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Molecular basis underlying cancer cell proliferation and T-cell anergy induction by diacylglycerol kinase alphaPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

[Journal Article] Membrane morphology determines diacylglycerol kinase alpha substrate acyl chain specificit2021

Author(s)

Journal Title

Data Source

[Journal Article] Membrane morphology determines diacylglycerol kinase alpha substrate acyl chain specificity.2021

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Palmitic acid- and/or palmitoleic acid-containing phosphatidic acids are generated by diacylglycerol kinase α in starved Jurkat T cells2020

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Crystal structure and calcium-induced conformational changes of diacylglycerol kinase α EF-hand domains2019

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Characterization of α-synuclein N-terminal domain as a novel cellular phosphatidic acid sensor2019

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Myristic acid specifically stabilizes diacylglycerol kinase delta protein in C2C12 skeletal muscle cells.2019

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Microarray analysis of gene expression in the diacylglycerol kinase η knockout mouse brain2019

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Expression, purification and characterization of human diacylglycerol kinase zeta2019

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Diacylglycerol kinase β induces filopodium formation via its C1, catalytic and carboxy-terminal domains and interacts with the Rac1-GTPase-activating protein, β2-chimaerin2018

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Where do substrates of diacylglycerol kinases come from? Diacylglycerol kinases utilize diacylglycerol species supplied from phosphatidylinositol turnover-independent pathways.2018

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Expression and purification of human diacylglycerol kinase α from baculovirus-infected insect cells for structural studies2018

Author(s)

Journal Title

Takahashi Daisuke 高橋大輔

[Presentation] α-シヌクレインの脂質結合能の解析とPAプローブへの応用2018