Ohishi Hiroaki 大石裕晃

Researcher Number	70912434
Other IDs	https://orcid.org/0000-0001-6672-4093
Affiliation (Current)	2026: 獨協医科大学, 医学部, 准教授
Affiliation (based on the past Project Information) *help	2022 – 2025: 九州大学, 生体防御医学研究所, 助教
Review Section/Research Field	Principal Investigator Basic Section 43050:Genome biology-related
Keywords	Principal Investigator 高次ゲノム構造 / 空間オミクス / エピゲノム / ライブセルイメージング / DNAメチル化 / 多様性 / ライブイメージング / ゲノム刷り込み / 転写バースト / インプリント遺伝子の転写動態 … More / RNA-seqFISH / mRNA / 新生RNA / リピート配列 / クロマチン構造変化 / DNAメチル化差異 / アレル特異的DNA検出法 / ゲノムインプリンティング / 粘性 / 転写の不均一性 / アレルの区別 Less

時空間ライブオミクスを実現するエピゲノムと高次ゲノム構造の同時可視化技術の開発Principal Investigator
- Principal Investigator
  
  大石裕晃
- Project Period (FY)
  2025 – 2027
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 43050:Genome biology-related
- Research Institution
  Kyushu University
Elucidation of the spatiotemporal regulatory mechanisms of genomic imprinting by fluorescence imagingPrincipal Investigator
- Principal Investigator
  
  Ohishi Hiroaki
- Project Period (FY)
  2022 – 2024
- Research Category
  
  Grant-in-Aid for Early-Career Scientists
- Review Section
  
  Basic Section 43050:Genome biology-related
- Research Institution
  Kyushu University

All 2025 2024 2023 2022

All Journal Article Presentation

[Journal Article] Anti-Phase Clustering of Regulatory Factors Shapes Gene Transcription Burst2025
- Author(s)
  Li Bitong、Wong Yew Yan、Flores-Rodriguez Neftali、Davidson Tara、Graus Matthew S.、Smialkovska Valeriia、Ohishi Hiroaki、Feldmann Angelika、Ochiai Hiroshi、Francois Mathias
- Journal Title
  
  bioRxiv
  
  Volume: -
- DOI
  10.2139/ssrn.5157778
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22K15084
[Journal Article] Transcription-coupled changes in genomic region proximities during transcriptional bursting2024
- Author(s)
  Ohishi Hiroaki、Shinkai Soya、Owada Hitoshi、Fujii Takeru、Hosoda Kazufumi、Onami Shuichi、Yamamoto Takashi、Ohkawa Yasuyuki、Ochiai Hiroshi
- Journal Title
  
  Science Advances
  
  Volume: 10 Issue: 49
- DOI
  10.1126/sciadv.adn0020
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PUBLICLY-23H04297, KAKENHI-PLANNED-24H02326, KAKENHI-PROJECT-23K23872, KAKENHI-PROJECT-22K15084, KAKENHI-PROJECT-22KJ2462
[Journal Article] Combined and differential roles of ADD domains of DNMT3A and DNMT3L on DNA methylation landscapes in mouse germ cells2024
- Author(s)
  Kubo Naoki、Uehara Ryuji、Uemura Shuhei、Ohishi Hiroaki、Shirane Kenjiro、Sasaki Hiroyuki
- Journal Title
  
  Nature Communications
  
  Volume: 15 Issue: 1 Pages: 3266-3266
- DOI
  10.1038/s41467-024-47699-2
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-22K15037, KAKENHI-PROJECT-22K15084, KAKENHI-PROJECT-24K01938, KAKENHI-PROJECT-24K09414
[Journal Article] Image Analysis Protocol for DNA/RNA/Immunofluorescence (IF)-seqFISH Data2024
- Author(s)
  Ohishi Hiroaki、Ochiai Hiroshi
- Journal Title
  
  Methods Mol Biol
  
  Volume: 2856 Pages: 419-432
- DOI
  10.1007/978-1-0716-4136-1_24
- ISBN
  9781071641354, 9781071641361
- Peer Reviewed
- Data Source
  KAKENHI-PLANNED-24H02326, KAKENHI-PROJECT-23K23872, KAKENHI-PROJECT-22K15084
[Journal Article] Precise immunofluorescence canceling for highly multiplexed imaging to capture specific cell states2024
- Author(s)
  Tomimatsu Kosuke、Fujii Takeru、Bise Ryoma、Hosoda Kazufumi、Taniguchi Yosuke、Ochiai Hiroshi、Ohishi Hiroaki、Ando Kanta、Minami Ryoma、Tanaka Kaori、Tachibana Taro、Mori Seiichi、Harada Akihito、Maehara Kazumitsu、Nagasaki Masao、Uchida Seiichi、Kimura Hiroshi、Narita Masashi、Ohkawa Yasuyuki
- Journal Title
  
  Nature Communications
  
  Volume: 15 Issue: 1 Pages: 3657-3657
- DOI
  10.1038/s41467-024-47989-9
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PLANNED-21H05292, KAKENHI-PUBLICLY-23H04288, KAKENHI-PLANNED-24H02323, KAKENHI-PLANNED-24H02326, KAKENHI-PROJECT-23H00372, KAKENHI-PROJECT-23K24795, KAKENHI-PROJECT-22K15084, KAKENHI-PROJECT-23K27087, KAKENHI-PROJECT-24K02334, KAKENHI-PROJECT-24K21949
[Journal Article] Transcription-coupled changes in higher-order genomic structure and transcription hub viscosity prolong enhancer-promoter connectivity2023
- Author(s)
  Ohishi Hiroaki、Shinkai Soya、Owada Hitoshi、Fujii Takeru、Hosoda Kazufumi、Onami Shuichi、Yamamoto Takashi、Ohkawa Yasuyuki、Ochiai Hiroshi
- Journal Title
  
  bioRxiv
  
  Volume: - Pages: 568629-568629
- DOI
  10.1101/2023.11.27.568629
- Open Access
- Data Source
  KAKENHI-PROJECT-22K15084, KAKENHI-PUBLICLY-22H04694, KAKENHI-PUBLICLY-23H04286, KAKENHI-PUBLICLY-23H04297, KAKENHI-PROJECT-23K23872
[Journal Article] The DNMT3A ADD domain is required for efficient de novo DNA methylation and maternal imprinting in mouse oocytes2023
- Author(s)
  Uehara Ryuji、Au Yeung Wan Kin、Toriyama Keisuke、Ohishi Hiroaki、Kubo Naoki、Toh Hidehiro、Suetake Isao、Shirane Kenjiro、Sasaki Hiroyuki
- Journal Title
  
  PLOS Genetics
  
  Volume: 19 Issue: 8 Pages: e1010855-e1010855
- DOI
  10.1371/journal.pgen.1010855
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-22K15037, KAKENHI-PROJECT-22K15084, KAKENHI-PROJECT-22K06174, KAKENHI-PROJECT-18K06095, KAKENHI-PROJECT-22K15125, KAKENHI-PUBLICLY-22H04675
[Journal Article] Organization of transcription and 3D genome as revealed by live-cell imaging2023
- Author(s)
  Ochiai Hiroshi、Ohishi Hiroaki、Sato Yuko、Kimura Hiroshi
- Journal Title
  
  Current Opinion in Structural Biology
  
  Volume: 81 Pages: 102615-102615
- DOI
  10.1016/j.sbi.2023.102615
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-22K15084, KAKENHI-PROJECT-20K06484, KAKENHI-PROJECT-21H04764
[Journal Article] Combined and differential roles of ADD domains of DNMT3A and DNMT3L on DNA methylation landscapes in mouse germ cells2023
- Author(s)
  Kubo Naoki、Uehara Ryuji、Uemura Shuhei、Ohishi Hiroaki、Shirane Kenjiro、Sasaki Hiroyuki
- Journal Title
  
  bioRxiv
  
  Volume: -
- DOI
  10.1101/2023.09.07.556008
- Data Source
  KAKENHI-PROJECT-22K15084
[Journal Article] Precise immunofluorescence canceling for highly multiplexed imaging capturing specific cell state2023
- Author(s)
  Tomimatsu Kosuke、Fujii Takeru、Bise Ryoma、Hosoda Kazufumi、Taniguchi Yosuke、Ochiai Hiroshi、Ohishi Hiroaki、Ando Kanta、Minami Ryoma、Tanaka Kaori、Tachibana Taro、Mori Seiichi、Harada Akihito、Maehara Kazumitsu、Nagasaki Masao、Uchida Seiichi、Kimura Hiroshi、Narita Masashi、Ohkawa Yasuyuki
- Journal Title
  
  bioRxiv
  
  Volume: -
- DOI
  10.1101/2023.10.17.561810
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-22K15084, KAKENHI-PROJECT-22K19275, KAKENHI-PROJECT-22K19314, KAKENHI-PUBLICLY-22H04676, KAKENHI-PUBLICLY-22H04694, KAKENHI-PLANNED-21H05292, KAKENHI-PUBLICLY-23H04286, KAKENHI-PROJECT-23H00372, KAKENHI-PROJECT-23K23872, KAKENHI-PROJECT-23K24795, KAKENHI-PROJECT-23K27087
[Journal Article] STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity2022
- Author(s)
  Ohishi Hiroaki、Shimada Seiru、Uchino Satoshi、Li Jieru、Sato Yuko、Shintani Manabu、Owada Hitoshi、Ohkawa Yasuyuki、Pertsinidis Alexandros、Yamamoto Takashi、Kimura Hiroshi、Ochiai Hiroshi
- Journal Title
  
  Nature Communications
  
  Volume: 13 Issue: 1 Pages: 7672-7672
- DOI
  10.1038/s41467-022-35286-2
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-20K06484, KAKENHI-PROJECT-22K19275, KAKENHI-PROJECT-22K15084, KAKENHI-PUBLICLY-21H05753, KAKENHI-PROJECT-20H00456, KAKENHI-PROJECT-21H04764, KAKENHI-PLANNED-18H05527, KAKENHI-PLANNED-18H05531, KAKENHI-PUBLICLY-21H00232, KAKENHI-PUBLICLY-22H04676, KAKENHI-PUBLICLY-22H04694, KAKENHI-PROJECT-23K23872
[Journal Article] STREAMING-Tag System: Technology to Enable Visualization of Transcriptional Activity and Subnuclear Localization of Specific Endogenous Genes2022
- Author(s)
  Ohishi Hiroaki、Ochiai Hiroshi
- Journal Title
  
  Methods in molecular biology
  
  Volume: 2577 Pages: 103-122
- DOI
  10.1007/978-1-0716-2724-2_8
- ISBN
  9781071627235, 9781071627242
- Data Source
  KAKENHI-PROJECT-22K15084, KAKENHI-PUBLICLY-21H05753
[Presentation] 高深度な空間オミクス技術の開発によるゲノムインプリンティング維持機構の解明2024
- Author(s)
  大石裕晃
- Organizer
  日本分子生物学会
- Data Source
  KAKENHI-PROJECT-22K15084
[Presentation] 転写活性化状態における遺伝子周辺の粘性上昇がエンハンサーとプロモーターの接触時間を延長させる2024
- Author(s)
  大石裕晃
- Organizer
  第 41 回染色体ワークショップ・第 22 回核ダイナミクス研究会
- Data Source
  KAKENHI-PROJECT-22K15084
[Presentation] STREAMING-tagによる転写活性と転写制御関連因子の時空間的な関連性の解明2023
- Author(s)
  大石裕晃
- Organizer
  第75回日本細胞生物学会大会
- Data Source
  KAKENHI-PROJECT-22K15084
[Presentation] 転写バーストにおける高次ゲノム構造と転写制御関連因子クラスターの空間的関係2023
- Author(s)
  大石裕晃
- Organizer
  第46回日本分子生物学会年会
- Data Source
  KAKENHI-PROJECT-22K15084
[Presentation] Single-gene imaging elucidates spatiotemporal relationships between transcriptional activity and regulatory factor clustering2022
- Author(s)
  大石裕晃、島田聖瑠、内野哲志、Jieru Li、佐藤優子、新谷学文、大和田一志、大川恭行、Pertsinidis Alexandros、山本卓、木村宏、落合博
- Organizer
  日本ゲノム編集学会第７回大会
- Data Source
  KAKENHI-PROJECT-22K15084

1. SHINKAI Soya

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
2. 佐藤優子

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
3. 久保直樹

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
4. 落合博

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
5. 原田哲仁

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results

Ohishi Hiroaki 大石 裕晃

Research Projects

Research Products

Co-Researchers

時空間ライブオミクスを実現するエピゲノムと高次ゲノム構造の同時可視化技術の開発Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Elucidation of the spatiotemporal regulatory mechanisms of genomic imprinting by fluorescence imagingPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

[Journal Article] Anti-Phase Clustering of Regulatory Factors Shapes Gene Transcription Burst2025

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Transcription-coupled changes in genomic region proximities during transcriptional bursting2024

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Combined and differential roles of ADD domains of DNMT3A and DNMT3L on DNA methylation landscapes in mouse germ cells2024

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Image Analysis Protocol for DNA/RNA/Immunofluorescence (IF)-seqFISH Data2024

Author(s)

Journal Title

DOI

ISBN

Data Source

[Journal Article] Precise immunofluorescence canceling for highly multiplexed imaging to capture specific cell states2024

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Transcription-coupled changes in higher-order genomic structure and transcription hub viscosity prolong enhancer-promoter connectivity2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] The DNMT3A ADD domain is required for efficient de novo DNA methylation and maternal imprinting in mouse oocytes2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Organization of transcription and 3D genome as revealed by live-cell imaging2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Combined and differential roles of ADD domains of DNMT3A and DNMT3L on DNA methylation landscapes in mouse germ cells2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Precise immunofluorescence canceling for highly multiplexed imaging capturing specific cell state2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity2022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] STREAMING-Tag System: Technology to Enable Visualization of Transcriptional Activity and Subnuclear Localization of Specific Endogenous Genes2022

Author(s)

Journal Title

DOI

ISBN

Data Source

[Presentation] 高深度な空間オミクス技術の開発によるゲノムインプリンテ ィング維持機構の解明2024

Author(s)

Ohishi Hiroaki 大石裕晃

[Presentation] 高深度な空間オミクス技術の開発によるゲノムインプリンティング維持機構の解明2024

[Presentation] 転写活性化状態における遺伝子周辺の粘性上昇がエンハンサーとプロモーターの接触時間を延長させる2024