Nishimura Kohei 西村浩平

… Alternative Names

NISHIMURA Kohei 西村浩平

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Researcher Number	80582709
Other IDs
Affiliation (Current)	2025: 名古屋大学, 理学研究科, 講師
Affiliation (based on the past Project Information) *help	2025: 名古屋大学, 理学研究科, 講師 2022 – 2023: 名古屋大学, 理学研究科, 講師 2019 – 2021: 名古屋大学, 理学研究科, 助教 2018: 大阪大学, 生命機能研究科, 特任助教(常勤) 2017: 大阪大学, 生命機能研究科, 特任助教
Review Section/Research Field	Principal Investigator Basic Section 38060:Applied molecular and cellular biology-related / Basic Section 43050:Genome biology-related / Genome biology Except Principal Investigator Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
Keywords	Principal Investigator ssAID法 / AID法 / オーキシン / 4C-seq / セントロメア / スクリーニング / ユビキチン / 標的タンパク質分解 / Auxin-Inuducible Degron法 / 短鎖型小分子抗体 … More / AlissAID / ナノボディ / ssAID / TIR1 / AID / タンパク質分解 / AlissAID法 / 細胞内タンパク質分解 / 動物培養細胞 / 出芽酵母 / Hi-C / ニワトリDT40 / ノックダウン / 核内構造 / セントロメア因子 / セントロメアタンパク質 / ネオセントロメア / DT40 / 染色体 / Auxin inducible degron / 4C法 / ヘテロクロマチン … More Except Principal Investigator 人為的制御 / 細胞周期 / Auxin Inducible Degron 法 / ユビキチンプロテアソーム / 動物培養細胞 / タンパク質分解 / CDK / 培養細胞 / 細胞周期制御 / AID法 / オーキシン Less

細胞内タンパク質分解に焦点を当てた小分子抗体スクリーニング技術の樹立Principal Investigator
- Principal Investigator
  
  西村浩平
- Project Period (FY)
  2025 – 2027
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 38060:Applied molecular and cellular biology-related
- Research Institution
  Nagoya University
タンパク質工学およびケミカルバイオロジーを駆使したタンパク質分解系の開発Principal Investigator
- Principal Investigator
  
  西村浩平
- Project Period (FY)
  2022 – 2024
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 38060:Applied molecular and cellular biology-related
- Research Institution
  Nagoya University
Artificial cell cycle control using the AID method, a low-toxicity and rapid proteolytic system
- Principal Investigator
  
  Kamura Takumi
- Project Period (FY)
  2020 – 2021
- Research Category
  
  Grant-in-Aid for Challenging Research (Exploratory)
- Review Section
  
  Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
- Research Institution
  Nagoya University
3D structural analysis of centromere in vertebrate cellsPrincipal Investigator
- Principal Investigator
  
  Nishimura Kohei
- Project Period (FY)
  2019 – 2022
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 43050:Genome biology-related
- Research Institution
  Nagoya University
3D structural analysis of centromere in vertebrate cellsPrincipal Investigator
- Principal Investigator
  
  NISHIMURA Kohei
- Project Period (FY)
  2017 – 2018
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Genome biology
- Research Institution
  Osaka University

All 2024 2023 2022 2021 2020 2019 2018 2017

All Journal Article Presentation

[Journal Article] E3 Ligases Regulate Organelle Inheritance in Yeast2024
- Author(s)
  Obara Keisuke、Nishimura Kohei、Kamura Takumi
- Journal Title
  
  Cells
  
  Volume: 13 Issue: 4 Pages: 292-292
- DOI
  10.3390/cells13040292
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22K05558, KAKENHI-PROJECT-23K27126
[Journal Article] Targeted Protein Degradation Systems: Controlling Protein Stability Using E3 Ubiquitin Ligases in Eukaryotic Species2024
- Author(s)
  Ogawa Yoshitaka、Ueda Taisei P.、Obara Keisuke、Nishimura Kohei、Kamura Takumi
- Journal Title
  
  Cells
  
  Volume: 13 Issue: 2 Pages: 175-175
- DOI
  10.3390/cells13020175
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22K05558, KAKENHI-PROJECT-23K27126
[Journal Article] Development of AlissAID system targeting GFP or mCherry fusion protein2023
- Author(s)
  Ogawa Yoshitaka、Nishimura Kohei、Obara Keisuke、Kamura Takumi
- Journal Title
  
  PLOS Genetics
  
  Volume: 19 Issue: 6 Pages: 1010731-1010747
- DOI
  10.1371/journal.pgen.1010731
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22K05558, KAKENHI-PROJECT-23K27126
[Journal Article] Development of AlissAID system targeting GFP or mCherry fusion protein2022
- Author(s)
  Yoshitaka Ogawa, Kohei Nishimura, Keisuke Obara, Takumi Kamura
- Journal Title
  
  BioRxiv
  
  Volume: なし
- DOI
  10.1101/2022.12.12.520189
- Open Access
- Data Source
  KAKENHI-PROJECT-22K05558
[Journal Article] Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase2022
- Author(s)
  Haraguchi Tokuko、Koujin Takako、Shindo Tomoko、Sukriye Bilir、Osakada Hiroko、Nishimura Kohei、Hirano Yasuhiro、Asakawa Haruhiko、Mori Chie、Kobayashi Shouhei、Okada Yasushi、Chikashige Yuji、Fukagawa Tatsuo、Shibata Shinsuke、Hiraoka Yasushi
- Journal Title
  
  Communications Biology
  
  Volume: 5 Issue: 1 Pages: 78-88
- DOI
  10.1038/s42003-022-03021-8
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-20K21423, KAKENHI-PLANNED-18H05528, KAKENHI-PLANNED-18H05533, KAKENHI-PUBLICLY-20H05389, KAKENHI-PLANNED-20H05891, KAKENHI-PUBLICLY-21H05752, KAKENHI-PROJECT-20H00454, KAKENHI-PROJECT-19H03394, KAKENHI-PROJECT-19K06611, KAKENHI-PROJECT-20K21395, KAKENHI-PROJECT-16H06280, KAKENHI-PROJECT-17H06167, KAKENHI-PLANNED-19H05795, KAKENHI-PROJECT-19K06489
[Journal Article] AID法と4C解析法を組み合わせた核内セントロメア構造の解析2022
- Author(s)
  西村浩平
- Journal Title
  
  月刊細胞
  
  Volume: 11 Pages: 55-58
- Data Source
  KAKENHI-PROJECT-19K06611
[Journal Article] 植物ホルモン・オーキシンによるタンパク質分解系(AID法)を利用した核内セントロメア構造の解析2022
- Author(s)
  西村浩平
- Journal Title
  
  アグリバイオ
  
  Volume: 6 Pages: 77-81
- Data Source
  KAKENHI-PROJECT-19K06611
[Journal Article] A Simple Method that Combines CRISPR and AID to Quickly Generate Conditional Knockouts for Essential Genes in Various Vertebrate Cell Lines2021
- Author(s)
  Nishimura Kohei、Fukagawa Tatsuo
- Journal Title
  
  Methods in molecular biology (Clifton, N.J.)
  
  Volume: 2377 Pages: 109-122
- DOI
  10.1007/978-1-0716-1720-5_6
- ISBN
  9781071617199, 9781071617205
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-20K21423, KAKENHI-PUBLICLY-20H05389, KAKENHI-PUBLICLY-21H05752, KAKENHI-PROJECT-19K06611, KAKENHI-PROJECT-20K21395, KAKENHI-PROJECT-17H06167
[Journal Article] A Simple Method to Generate Super-sensitive AID (ssAID)-based Conditional Knockouts using CRISPR-based Gene Knockout in Various Vertebrate Cell Lines2021
- Author(s)
  Nishimura Kohei、Fukagawa Tatsuo
- Journal Title
  
  BIO-PROTOCOL
  
  Volume: 11 Issue: 14
- DOI
  10.21769/bioprotoc.4092
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-20K21423, KAKENHI-PUBLICLY-20H05389, KAKENHI-PUBLICLY-21H05752, KAKENHI-PROJECT-19K06611, KAKENHI-PROJECT-20K21395, KAKENHI-PROJECT-17H06167
[Journal Article] CUL2LRR1 , TRAIP and p97 control CMG helicase disassembly in the mammalian cell cycle.2021
- Author(s)
  Fabrizio Villa , Ryo Fujisawa , Johanna Ainsworth , Kohei Nishimura , Michael Lie-A-Ling , Georges Lacaud , Karim Pm Labib
- Journal Title
  
  EMBO reports
  
  Volume: 5 Issue: 3
- DOI
  10.15252/embr.202052164
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K06611, KAKENHI-PROJECT-20K21423
[Journal Article] A super sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair2020
- Author(s)
  Kohei Nishimura, Ryotaro Yamada, Shinya Hagihara, Rie Iwasaki, Naoyuki Uchida, Takumi Kamura, Koji Takahashi, Keiko U. Torii, Tatsuo Fukagawa
- Journal Title
  
  BioRxiv
  
  Volume: 0 Pages: 0-0
- DOI
  10.1101/2020.01.20.912113
- Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K06611
[Journal Article] A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair.2020
- Author(s)
  Kohei Nishimura , Ryotaro Yamada , Shinya Hagihara , Rie Iwasaki , Naoyuki Uchida , Takumi Kamura , Koji Takahashi , Keiko U Torii , Tatsuo Fukagawa
- Journal Title
  
  Nucleic acids research
  
  Volume: 48 Issue: 18 Pages: e108-e108
- DOI
  10.1093/nar/gkaa748
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K06611, KAKENHI-PROJECT-20K21395, KAKENHI-PROJECT-20K21422, KAKENHI-PROJECT-20K21423, KAKENHI-PROJECT-17H06167, KAKENHI-PLANNED-17H06350, KAKENHI-PLANNED-17H06476, KAKENHI-PUBLICLY-20H04883, KAKENHI-PUBLICLY-20H05389, KAKENHI-PUBLICLY-20H05409, KAKENHI-PROJECT-19H00990, KAKENHI-PROJECT-17H03695, KAKENHI-PROJECT-18H02085, KAKENHI-PROJECT-20H03208, KAKENHI-ORGANIZER-19H05670, KAKENHI-PROJECT-17KT0017
[Journal Article] Essentiality of CENP-A Depends on Its Binding Mode to HJURP.2020
- Author(s)
  Tetsuya Hori , JingHui Cao , Kohei Nishimura , Mariko Ariyoshi , Yasuhiro Arimura , Hitoshi Kurumizaka , Tatsuo Fukagawa
- Journal Title
  
  Cell reports
  
  Volume: 33 Issue: 7 Pages: 108388-108388
- DOI
  10.1016/j.celrep.2020.108388
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K06611, KAKENHI-PROJECT-20K21395, KAKENHI-PROJECT-20K21423, KAKENHI-PROJECT-17H06167, KAKENHI-PLANNED-18H05534, KAKENHI-PUBLICLY-20H05389, KAKENHI-PROJECT-20H03236, KAKENHI-PROJECT-18K06084, KAKENHI-PROJECT-16H06279
[Journal Article] 3D genomic architecture reveals that neocentromeres associate with heterochromatin regions2018
- Author(s)
  Nishimura Kohei、Komiya Masataka、Hori Tetsuya、Itoh Takehiko、Fukagawa Tatsuo
- Journal Title
  
  The Journal of Cell Biology
  
  Volume: 218 Issue: 1 Pages: 134-149
- DOI
  10.1083/jcb.201805003
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K07501, KAKENHI-PLANNED-15H05972, KAKENHI-PLANNED-15H05979, KAKENHI-PROJECT-17H06167, KAKENHI-PROJECT-17K15041, KAKENHI-PROJECT-16H06279
[Journal Article] Acute Inactivation of the Replicative Helicase in Human Cells Triggers MCM8-9-dependent DNA Synthesis2017
- Author(s)
  Natsume T, Nishimura K, Minocherhomji S, Bhwmick R, Hickson ID, Kanemaki MT
- Journal Title
  
  Genes & Development
  
  Volume: advanced online Issue: 8 Pages: 816-829
- DOI
  10.1101/gad.297663.117
- Peer Reviewed / Acknowledgement Compliant / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16K15095, KAKENHI-PROJECT-17K15041, KAKENHI-PROJECT-17K15068
[Journal Article] An efficient method to generate conditional knockout cell lines for essential genes by combination of auxin-inducible degron tag and CRISPR/Cas9.2017
- Author(s)
  Nishimura K, Fukagawa T
- Journal Title
  
  Chromosome Res.
  
  Volume: 25 Issue: 3-4 Pages: 816-829
- DOI
  10.1007/s10577-017-9559-7
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-17K15041, KAKENHI-PLANNED-15H05972, KAKENHI-PROJECT-17H06167, KAKENHI-PROJECT-16H06279
[Presentation] Auxin-Inducible Degron（AID）法を用いた細胞内タンパク質分解コントロール2023
- Author(s)
  西村浩平
- Organizer
  日本プロテオーム学会2023年大会
- Invited
- Data Source
  KAKENHI-PROJECT-22K05558
[Presentation] 小分子抗体を用いて標的タンパク質を分解する「AlissAID法」の開発2023
- Author(s)
  小川佳孝, 西村浩平, 小原圭介, 嘉村巧
- Organizer
  日本遺伝学会　第95回大会
- Data Source
  KAKENHI-PROJECT-22K05558
[Presentation] Snf1/AMPK依存性糖シグナル経路におけるHxk2のリン酸化機構2023
- Author(s)
  野中一輝, 深瀬文奈子, 小原圭介, 西村浩平, 嘉村巧
- Organizer
  第46回日本分子生物学会
- Data Source
  KAKENHI-PROJECT-22K05558
[Presentation] 小分子抗体を利用した標的タンパク質分解系「AlissAID」システムの開発2023
- Author(s)
  小川佳孝, 西村浩平, 小原圭介, 嘉村巧
- Organizer
  第46回日本分子生物学会
- Data Source
  KAKENHI-PROJECT-22K05558
[Presentation] 第45回日本分子生物学会年会2022
- Author(s)
  西村浩平、小川佳孝、福原佳乃、小原圭介、嘉村巧
- Organizer
  真核生物種におけるオーキシン依存的なタンパク質分解系の利用法
- Data Source
  KAKENHI-PROJECT-22K05558
[Presentation] 第45回日本分子生物学会年会2022
- Author(s)
  小川佳孝, 西村浩平, 小原圭介, 嘉村巧
- Organizer
  迅速かつ低毒性に標的タンパク質を分解する AlissAIDシステムの開発
- Data Source
  KAKENHI-PROJECT-22K05558
[Presentation] Auxin Inducible Degron (AID) 株を利用したオーキシン分子のスクリーニング2021
- Author(s)
  福原佳乃、中島優希、角房直哉、佐藤綾人、打田直行、小原圭介、西村浩平、嘉村巧
- Organizer
  第62回日本植物整理学会年会
- Data Source
  KAKENHI-PROJECT-20K21423
[Presentation] 改良型オーキシンと受容体を用いた高感度タンパク質分解法2021
- Author(s)
  西村浩平
- Organizer
  日本遺伝学会第93回大会
- Data Source
  KAKENHI-PROJECT-20K21423
[Presentation] 出芽酵母Tsk1はSnf1依存的低グルコース応答シグナルの負の制御因子である2021
- Author(s)
  深瀨文奈子、佐々木怜菜、小原圭介、西村浩平、嘉村巧
- Organizer
  第44回日本分子生物学会年会
- Data Source
  KAKENHI-PROJECT-20K21423
[Presentation] Auxin Inducible Degron (AID) 株を利用したオーキシン分子のスクリーニング2021
- Author(s)
  福原佳乃、中島優希、角房直哉、佐藤綾人、打田直行、小原圭介、嘉村巧、西村浩平
- Organizer
  第62回日本植物整理学会年会
- Data Source
  KAKENHI-PROJECT-19K06611
[Presentation] 改良型オーキシンと受容体を用いた高感度タンパク質分解法2021
- Author(s)
  西村浩平
- Organizer
  日本遺伝学会第93回大会
- Data Source
  KAKENHI-PROJECT-19K06611
[Presentation] A super-sensitive Auxin Inducible Degron (ssAID) system for conditional knockdown in various vertebrate cell lines2021
- Author(s)
  Kohei Nishimura
- Organizer
  第44回日本分子生物学会年会
- Data Source
  KAKENHI-PROJECT-20K21423
[Presentation] A super-sensitive Auxin-Inducible Degron (ssAID) system for conditional knockdown in various vertebrate cell lines2021
- Author(s)
  Nishimura K
- Organizer
  2nd Webinar on Nucleic Acid and CRISPR
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-20K21423
[Presentation] A super-sensitive Auxin-Inducible Degron (ssAID) system for conditional knockdown in various vertebrate cell lines2021
- Author(s)
  Kohei Nishimura
- Organizer
  2nd Webinar on Nucleic Acid and CRISPR
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K06611
[Presentation] 人工オーキシンとTIR1のペアを用いた高感受性AID法2020
- Author(s)
  西村浩平、山田遼太郎、萩原伸也、岩崎理恵、打田直行、嘉村巧、高橋宏二、鳥居恵子、深川竜郎
- Organizer
  第43回日本分子生物学会
- Data Source
  KAKENHI-PROJECT-20K21423
[Presentation] A super-sensitive Auxin-Inducible Degron system for conditional knockdown in various vertebrate cell lines2020
- Author(s)
  Kohei Nishimura , Ryotaro Yamada , Shinya Hagihara , Rie Iwasaki , Naoyuki Uchida , Takumi Kamura , Koji Takahashi , Keiko U Torii , Tatsuo Fukagawa
- Organizer
  第43回日本分子生物学会年会
- Data Source
  KAKENHI-PROJECT-19K06611
[Presentation] 脊椎動物細胞におけるセントロメアの3次元構造解析2019
- Author(s)
  西村浩平, 堀哲也, 豊田敦, 古宮正隆, 伊藤武彦, 深川竜郎
- Organizer
  日本遺伝学会大会
- Data Source
  KAKENHI-PROJECT-19K06611
[Presentation] 4C解析による核内セントロメア構造分子基盤とその役割の解明2019
- Author(s)
  西村浩平
- Organizer
  第36回　染色体ワークショップ
- Data Source
  KAKENHI-PROJECT-17K15041
[Presentation] Easy construction of Auxin Inducible Degron mutants in vertebrate cells2018
- Author(s)
  Kohei Nishimura
- Organizer
  Genome Editing and Functional Genomics 2018
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K15041
[Presentation] ネオセントロメアから迫るセントロメア領域における染色体構造の解析2018
- Author(s)
  西村浩平
- Organizer
  第41回　日本分子生物学会年会
- Data Source
  KAKENHI-PROJECT-17K15041
[Presentation] ネオセントロメアから迫るセントロメア領域における染色体構造の解析2017
- Author(s)
  Kohei Nishimura
- Organizer
  日本分子生物学会年会
- Data Source
  KAKENHI-PROJECT-17K15041
[Presentation] 3D structural analysis of neo-centromere region in vertebrate cell2017
- Author(s)
  Kohei Nishimura
- Organizer
  EMBP Workshop Dynamic Kinetochore
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K15041

1. Kamura Takumi (40333455)

# of Collaborated Projects: 1 results

# of Collaborated Products: 3 results
2. HORI Tetsuya

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
3. 鐘巻将人

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results

Nishimura Kohei 西村 浩平

NISHIMURA Kohei 西村 浩平

Research Projects

Research Products

Co-Researchers

細胞内タンパク質分解に焦点を当てた小分子抗体スクリーニング技術の樹立Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

タンパク質工学およびケミカルバイオロジーを駆使したタンパク質分解系の開発Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Artificial cell cycle control using the AID method, a low-toxicity and rapid proteolytic system

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

3D structural analysis of centromere in vertebrate cellsPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

3D structural analysis of centromere in vertebrate cellsPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

[Journal Article] E3 Ligases Regulate Organelle Inheritance in Yeast2024

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Targeted Protein Degradation Systems: Controlling Protein Stability Using E3 Ubiquitin Ligases in Eukaryotic Species2024

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Development of AlissAID system targeting GFP or mCherry fusion protein2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Development of AlissAID system targeting GFP or mCherry fusion protein2022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase2022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] AID法と4C解析法を組み合わせた核内セントロメア構造の解析2022

Author(s)

Journal Title

Data Source

[Journal Article] 植物ホルモン・オーキシンによるタンパク質分解系(AID法)を利用した核内セントロメア構造の解析2022

Author(s)

Journal Title

Data Source

[Journal Article] A Simple Method that Combines CRISPR and AID to Quickly Generate Conditional Knockouts for Essential Genes in Various Vertebrate Cell Lines2021

Author(s)

Journal Title

DOI

ISBN

Data Source

[Journal Article] A Simple Method to Generate Super-sensitive AID (ssAID)-based Conditional Knockouts using CRISPR-based Gene Knockout in Various Vertebrate Cell Lines2021

Author(s)

Journal Title

DOI

Data Source

[Journal Article] CUL2LRR1 , TRAIP and p97 control CMG helicase disassembly in the mammalian cell cycle.2021

Nishimura Kohei 西村浩平

NISHIMURA Kohei 西村浩平

[Presentation] Auxin Inducible Degron (AID) 株を利用したオーキシン分子のスクリーニング2021