Tsunekawa Yuji 恒川雄二

… Alternative Names

Yuji Tsunekawa 恒川雄二

Less

Researcher Number	80733352
Other IDs
Affiliation (Current)	2026: 東京大学, 医科学研究所, 助教
Affiliation (based on the past Project Information) *help	2020 – 2025: 東京大学, 医科学研究所, 助教 2018 – 2019: 国立研究開発法人理化学研究所, 生命機能科学研究センター, 研究員 2016 – 2017: 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, 研究員
Review Section/Research Field	Principal Investigator Basic Section 43060:System genome science-related / Basic Section 42040:Laboratory animal science-related / Medium-sized Section 43:Biology at molecular to cellular levels, and related fields / Nerve anatomy/Neuropathology Except Principal Investigator Medium-sized Section 56:Surgery related to the biological and sensory functions and related fields
Keywords	Principal Investigator Cre de novo ノックイン / HBoV vector / リコンビネース / ノックイン / Creノックイン / LsCre / AAV / LsCreの開発 / LeaklessCre / de novo Cre knock-in … More / 複雑脳の発生 / de novoノックイン技術の開発 / 複雑脳におけるAscl1の機能解析 / de novo Cre KI / 複雑脳発生 … More Except Principal Investigator 能動免疫療法 / DMD / 色素性乾皮症 / 増幅型MSC / 自殺遺伝子療法 / 神経変性疾患 / AAVベクター / MSC / AAV / 遺伝子治療 Less

組織特異的ヒトボカウイルスベクターの開発Principal Investigator
- Principal Investigator
  
  恒川雄二
- Project Period (FY)
  2025 – 2027
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 43060:System genome science-related
- Research Institution
  The University of Tokyo
アデノ随伴ウイルスベクター基盤技術開発と脳神経疾患に対する遺伝子細胞療法
- Principal Investigator
  
  岡田尚巳
- Project Period (FY)
  2024 – 2028
- Research Category
  
  Grant-in-Aid for Scientific Research (A)
- Review Section
  
  Medium-sized Section 56:Surgery related to the biological and sensory functions and related fields
- Research Institution
  The University of Tokyo
Development of novel recombinant enzyme for understanding complex brain developmentPrincipal Investigator
- Principal Investigator
  
  Tsunekawa Yuji
- Project Period (FY)
  2020 – 2023
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 42040:Laboratory animal science-related
- Research Institution
  The University of Tokyo
Developing new Cre-loxp system for all organismPrincipal Investigator
- Principal Investigator
  
  Tsunekawa Yuji
- Project Period (FY)
  2018 – 2019
- Research Category
  
  Grant-in-Aid for Challenging Research (Exploratory)
- Review Section
  
  Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
- Research Institution
  Institute of Physical and Chemical Research
Study of molecular mechanism of complex brain by ferretPrincipal Investigator
- Principal Investigator
  
  Yuji Tsunekawa
- Project Period (FY)
  2016 – 2017
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Nerve anatomy/Neuropathology
- Research Institution
  Institute of Physical and Chemical Research

All 2025 2024 2023 2022 2019 2017 2016

All Journal Article Presentation Patent

[Journal Article] Enhanced Discriminability of Viral Vectors in Viscous Nanopores2025
- Author(s)
  Tsutsui Makusu、Tsunekawa Yuji、Wada Mikako、Arima Akihide、Onodera Azusa、Nishina Masumi、Nagoya Miho、Baba Yoshinobu、Kawai Tomoji、Okada Takashi
- Journal Title
  
  Small Methods
  
  Volume: - Issue: 7 Pages: 2401321-2401321
- DOI
  10.1002/smtd.202401321
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-24H00646, KAKENHI-PROJECT-23K23194, KAKENHI-PROJECT-24K01511
[Journal Article] Characterization of disease-specific alterations in metabolites and effects of mesenchymal stromal cells on dystrophic muscles2024
- Author(s)
  Nitahara-Kasahara Yuko、Posadas-Herrera Guillermo、Hirai Kunio、Oda Yuki、Snagu-Miyamoto Noriko、Yamanashi Yuji、Okada Takashi
- Journal Title
  
  Frontiers in Cell and Developmental Biology
  
  Volume: 12
- DOI
  10.3389/fcell.2024.1363541
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-24H00646
[Journal Article] Purification of Adeno‐Associated Viral Vector Serotype 9 Using Ceramic Hydroxyapatite Chromatography and its Analysis2024
- Author(s)
  Kurosawa Yae、Tsunekawa Yuji、Wada Mikako、Aizen Yuko、Nitahara‐Kasahara Yuko、Okada Takashi
- Journal Title
  
  Current Protocols
  
  Volume: 4 Issue: 6
- DOI
  10.1002/cpz1.1068
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-24H00646
[Journal Article] Identifying Viral Vector Characteristics by Nanopore Sensing2024
- Author(s)
  Tsutsui Makusu、Wada Mikako、Arima Akihide、Tsunekawa Yuji、Sasaki Takako、Sakamoto Kenji、Yokota Kazumichi、Baba Yoshinobu、Kawai Tomoji、Okada Takashi
- Journal Title
  
  ACS Nano
  
  Volume: 18 Issue: 24 Pages: 15695-15704
- DOI
  10.1021/acsnano.4c01888
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-24H00646, KAKENHI-PROJECT-23K23194, KAKENHI-PROJECT-22K04893
[Journal Article] Subcellular mRNA localization and local translation of Arhgap11a in radial glial progenitors regulates cortical development2023
- Author(s)
  Pilaz LJ, Liu J, Joshi K, Tsunekawa Y, Musso CM, D'Arcy BR, Suzuki IK, Alsina FC, Kc P, Sethi S, Vanderhaeghen P, Polleux F, Silver DL.
- Journal Title
  
  Neuron .
  
  Volume: 111(6) Issue: 6 Pages: 839-856
- DOI
  10.1016/j.neuron.2023.02.023
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-20K06464, KAKENHI-PROJECT-23K23891
[Journal Article] Engineering a highly durable adeno-associated virus receptor for analytical applications2023
- Author(s)
  Yoshida Kouhei、Tsunekawa Yuji、Kurihara Kento、Watanabe Kazuya、Makino-Manabe Yuriko、Wada Mikako、Tanaka Toru、Ide Teruhiko、Okada Takashi
- Journal Title
  
  Molecular Therapy - Methods & Clinical Development
  
  Volume: 31 Pages: 101157-101157
- DOI
  10.1016/j.omtm.2023.101157
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-20K06464, KAKENHI-PROJECT-23K20328
[Journal Article] Large-scale purification of functional AAV particles packaging the full genome using short-term ultracentrifugation with a zonal rotor2023
- Author(s)
  Wada M, Uchida N, Posadas-Herrera G, Hayashita-Kinoh H, Tsunekawa Y, Hirai Y, Okada T.
- Journal Title
  
  Gene Therapy
  
  Volume: Mar 28 Issue: 7-8 Pages: 641-648
- DOI
  10.1038/s41434-023-00398-x
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-23K20328, KAKENHI-PROJECT-22K06064, KAKENHI-PROJECT-20K06464
[Journal Article] Development of a novel purification method for AAV vectors using tangential flow filtration2023
- Author(s)
  Miyaoka Rimi、Tsunekawa Yuji、Kurosawa Yae、Sasaki Takako、Onodera Azusa、Sakamoto Kenji、Kakiuchi Yuko、Wada Mikako、Nitahara‐Kasahara Yuko、Hayashita‐Kinoh Hiromi、Okada Takashi
- Journal Title
  
  Biotechnology and Bioengineering
  
  Volume: 120 Issue: 11 Pages: 3311-3321
- DOI
  10.1002/bit.28524
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-22K06064, KAKENHI-PROJECT-22K06921, KAKENHI-PROJECT-20K06464, KAKENHI-PROJECT-23K20328
[Journal Article] In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration.2016
- Author(s)
  Suzuki K, Tsunekawa Y, Hernandez-Benitez R, Wu J, Zhu J, Kim EJ, Hatanaka F, Yamamoto M, Araoka T, Li Z, Kurita M, Hishida T, Li M, Aizawa E, Guo S, Chen S, Goebl A, Soligalla RD, Qu J, Jiang T, Fu X, ---, Belmonte JC.
- Journal Title
  
  Nature
  
  Volume: 540(7631) Issue: 7631 Pages: 144-149
- DOI
  10.1038/nature20565
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16K18382
[Journal Article] Developing a De Novo Targeted Knock-in Method Based on in Utero Electroporation Into the Mammalian Brain2016
- Author(s)
  Tsunekawa, Yuji, Raymond Kunikane Terhune, Ikumi Fujita, Atsunori Shitamukai, Taeko Suetsugu, Fumio Matsuzaki
- Journal Title
  
  Development
  
  Volume: 143 Issue: 17 Pages: 3216-3222
- DOI
  10.1242/dev.136325
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-16K18381, KAKENHI-PROJECT-16K18382, KAKENHI-PROJECT-16K21627
[Patent] 接着性細胞の浮遊培養用基材2025
- Inventor(s)
  恒川雄二、岡田尚巳、佐々木皓平、小島理恵
- Industrial Property Rights Holder
  恒川雄二、岡田尚巳、佐々木皓平、小島理恵
- Industrial Property Rights Type
  特許
- Industrial Property Number
  2024-044951
- Filing Date
  2025
- Data Source
  KAKENHI-PROJECT-24H00646
[Patent] ウイルスの精製方法2022
- Inventor(s)
  恒川雄二
- Industrial Property Rights Holder
  恒川雄二
- Industrial Property Rights Type
  特許
- Industrial Property Number
  2022-047745
- Filing Date
  2022
- Data Source
  KAKENHI-PROJECT-20K06464
[Patent] ポリヌクレオチドおよびその利用2019
- Inventor(s)
  恒川雄二
- Industrial Property Rights Holder
  恒川雄二
- Industrial Property Rights Type
  特許
- Filing Date
  2019
- Data Source
  KAKENHI-PROJECT-18K19311
[Patent] US provisional application 62/363,1642016
- Inventor(s)
  Yuji Tsunekawa
- Industrial Property Rights Holder
  Yuji Tsunekawa
- Industrial Property Rights Type
  特許
- Filing Date
  2016-07-15
- Overseas
- Data Source
  KAKENHI-PROJECT-16K18382
[Presentation] AAV 中空粒子にパッケージングされた AD- 発現カセットは、in vivo において長期発現が可能である2024
- Author(s)
  喜納裕美、ポサダス・エレラ・ギジェルモ、長野あゆ美、恒川雄二、岡田尚巳
- Organizer
  第30回日本遺伝子細胞治療学会学術集会
- Data Source
  KAKENHI-PROJECT-24H00646
[Presentation] 遺伝子治療用製品製造に向けた大規模超遠心による組換えアデノ随伴ウイルス完全体分離法の開発2024
- Author(s)
  和田美加子、恒川雄二、菅生健、平井幸彦、ポサダス・エレラ・ギジェルモ、喜納（早下）裕美、岡田尚巳
- Organizer
  第30回日本遺伝子細胞治療学会学術集会
- Data Source
  KAKENHI-PROJECT-24H00646
[Presentation] Development of nucleic acid vaccine with AAV empty capsids and exosomes（AAV関連技術を活用した次世代ワクチンの開発）2024
- Author(s)
  Tsunekawa Y, Sugo K, Matsuzaka Y, Kinoh H, Ueki H, Yamayoshi S, Okada T.
- Organizer
  第2回AMED SCARDA「ワクチン開発のための世界トップレベル研究開発拠点の形成事業」合同シンポジウム
- Data Source
  KAKENHI-PROJECT-24H00646
[Presentation] Improvement of Transduction Efficiency of Adeno-Associated Vector (AAV) by Extracellular Vesicles (EVs).2024
- Author(s)
  Matsuzaka Y, Wada M, Kobari Y, Sugo K, Tsunekawa Y, Posadas-Herrera G, Kodera H, Hayashita-Kinoh H, Okada T.
- Organizer
  27th ASGCT Annual Meeting
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-24H00646
[Presentation] Development of Novel HBoV vector vaccine（次世代ヒトボカウイルスベクターワクチンの開発に関する基礎研究）2024
- Author(s)
  Tsunekawa Y, Ito J, Okada T.
- Organizer
  第2回AMED SCARDA「ワクチン開発のための世界トップレベル研究開発拠点の形成事業」合同シンポジウム
- Data Source
  KAKENHI-PROJECT-24H00646
[Presentation] Amplifying AAV vector transduction efficacy with transgenic cell-driven compound discovery2024
- Author(s)
  Posadas-Herrera G, Tsunekawa Y, Okada T.
- Organizer
  The 30th Annual Meeting of Japan Society of Gene and Cell Therapy
- Data Source
  KAKENHI-PROJECT-24H00646
[Presentation] Short-Term and Large-Scale Production of High-Quality Recombinant Adeno-Associated Virus Using a Zonal Ultracentrifugation.2024
- Author(s)
  Wada M, Hirai Y, Sugo, K Tsunekawa Y, Posadas-Herrera G, Hayashita-Kinoh H, Nagoya M, Nishina M, Okada T.
- Organizer
  27th ASGCT Annual Meeting
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-24H00646
[Presentation] ベクソソームを用いた新規ウイルスベクターワクチンの開発2024
- Author(s)
  松坂恭成、菅生健、古寺紘人、和田美加子、喜納裕美、恒川雄二、岡田尚巳
- Organizer
  30回日本遺伝子細胞治療学会学術集会
- Data Source
  KAKENHI-PROJECT-24H00646
[Presentation] 効果的に免疫を誘導する新規ウイルスベクターワクチンの規格検証2024
- Author(s)
  菅生健、恒川雄二、喜納（早下）裕美、岡田尚巳
- Organizer
  第30回日本遺伝子細胞治療学会学術集会
- Data Source
  KAKENHI-PROJECT-24H00646
[Presentation] AAV ベクター感染増強剤の開発に向けた基礎研究2024
- Author(s)
  早川准平、恒川雄二、岡田尚巳
- Organizer
  第30回日本遺伝子細胞治療学会学術集会
- Data Source
  KAKENHI-PROJECT-24H00646
[Presentation] Enhancing the efficacy of AAV-based gene therapy and vaccines: Use of transgenic cells for high-throughput screening of compounds that enhance transgene expression.2024
- Author(s)
  Posadas-Herrera G, Tsunekawa Y, Nagano A, Onodera A, Hayakawa J, Okada T.
- Organizer
  The 2nd UTOPIA Symposium
- Data Source
  KAKENHI-PROJECT-24H00646
[Presentation] Developing de novo genome editing meyhods2017
- Author(s)
  恒川雄二
- Organizer
  Conbio2017
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16K18382

1. 宮道和成 (30612577)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
2. 岡田尚巳 (00326828)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
3. 曽田泰 (00361618)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
4. 喜納裕美 (60532728)

# of Collaborated Projects: 1 results

# of Collaborated Products: 8 results
5. 笠原優子 (90391911)

# of Collaborated Projects: 1 results

# of Collaborated Products: 2 results
6. 松坂恭成 (60420949)

# of Collaborated Projects: 1 results

# of Collaborated Products: 3 results
7. 伊東潤平 (20835540)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
8. 山口貴世志 (50466843)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
9. 岡田尚已

# of Collaborated Projects: 0 results

# of Collaborated Products: 16 results
10. 下向敦範

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results

Tsunekawa Yuji 恒川 雄二

Yuji Tsunekawa 恒川 雄二

Research Projects

Research Products

Co-Researchers

組織特異的ヒトボカウイルスベクターの開発Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

アデノ随伴ウイルスベクター基盤技術開発と脳神経疾患に対する遺伝子細胞療法

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Development of novel recombinant enzyme for understanding complex brain developmentPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Developing new Cre-loxp system for all organismPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Study of molecular mechanism of complex brain by ferretPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

[Journal Article] Enhanced Discriminability of Viral Vectors in Viscous Nanopores2025

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Characterization of disease-specific alterations in metabolites and effects of mesenchymal stromal cells on dystrophic muscles2024

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Purification of Adeno‐Associated Viral Vector Serotype 9 Using Ceramic Hydroxyapatite Chromatography and its Analysis2024

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Identifying Viral Vector Characteristics by Nanopore Sensing2024

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Subcellular mRNA localization and local translation of Arhgap11a in radial glial progenitors regulates cortical development2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Engineering a highly durable adeno-associated virus receptor for analytical applications2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Large-scale purification of functional AAV particles packaging the full genome using short-term ultracentrifugation with a zonal rotor2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Development of a novel purification method for AAV vectors using tangential flow filtration2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration.2016

Author(s)

Journal Title

DOI

Data Source

Tsunekawa Yuji 恒川雄二

Yuji Tsunekawa 恒川雄二