TSUBOUCHI HIDEO 坪内英生

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TSUBOUCHI Hideo 坪内英生

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Researcher Number	20283822
Other IDs	https://orcid.org/0000-0003-0814-8432
External Links
Affiliation (Current)	2025: 東京科学大学, 総合研究院, 助教
Affiliation (based on the past Project Information) *help	2017 – 2024: 東京工業大学, 科学技術創成研究院, 助教 2016: 基礎生物学研究所, 幹細胞生物学研究室, 特別協力研究員 1996 – 1998: 大阪大学, 大学院・理学研究科, 助手
Review Section/Research Field	Principal Investigator Basic Section 43010:Molecular biology-related / Molecular biology Except Principal Investigator Biological Sciences
Keywords	Principal Investigator 相同組換え / DNA傷害修復 / Rad51 / 減数分裂 / ゲノム恒常性 / 担子菌 / 乳がん・卵巣がん症候群 / ナガニシア酵母 / BRCA2 / 遺伝性乳がん・卵巣がん … More / ゲノム安定性 / ＲｅｃＡ / ＤＮＡ傷害修復 / RecA / DNA修復 / Dmc1 / RecAホモログ / 分裂酵母 / DNA二重鎖切断 / 遺伝学 / 出芽酵母 / 染色体構造 / 細胞周期 / 染色体 / シナプトネマ複合体 … More Except Principal Investigator processing from DSB ends / initiation of meiotic recombination / REP protein / Rad52 protein / strand-transfer activity of Rad51 / RecA protein / nuclease activity of Mre11 protein / non-homologous end joining / キメラRecA蛋白質 / RecA蛋白のDNA結合領域 / DNA二重鎖切断 / 染色体構造 / 組換え開始機構 / Lim15(Dmc1)蛋白質 / Rad51蛋白質 / Rad51,Lim15の染色体上の局在 / DNA鎖移行反応 / Rad52-RPA複合体 / 減数分裂期二重鎖切断 / Rad50-Mre11-Xrs2複合体 / クロマチン構造変化 / 組喚えの開始機構 / 遺伝的相同組喚え / テロメア領域の組換え / Rad51とLim15の染色体上の局在 / Rad51-Rad52-RPA複合体 / 交叉型組換え体形成MER3遺伝子 / 減数分裂期特異的スプライシング / MRE2遺伝子の機能 / 二本鎖DNA結合"gateway"モデル / RecA蛋白質のC末ドメイン / MRE2とスプライシング / MRE11,RAD50 & XRS2遺伝子 / MRE11,RAD50&XRS2遺伝子 / RPA / Rad51とRad52の相互作用 / 非相同組換え / MER3遺伝子 / MRE11, RAD50 & XRS2遺伝子 / DNA二重鎖切断修復 / 減数分裂期組換え / 出芽酵母 Less

ナガニシア酵母で解き明かす相同組換え制御機構Principal Investigator
- Principal Investigator
  
  坪内英生
- Project Period (FY)
  2023 – 2027
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 43010:Molecular biology-related
- Research Institution
  Institute of Science Tokyo
Similarity and difference between Rad51 and Dmc1, two RecA homologsPrincipal Investigator
- Principal Investigator
  
  Tsubouchi Hideo
- Project Period (FY)
  2018 – 2021
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 43010:Molecular biology-related
- Research Institution
  Tokyo Institute of Technology
Understanding the mechanisms for ensuring timely destruction of the synaptonemal complex during meiosisPrincipal Investigator
- Principal Investigator
  
  TSUBOUCHI Hideo
- Project Period (FY)
  2016 – 2017
- Research Category
  
  Grant-in-Aid for Research Activity Start-up
- Research Field
  
  Molecular biology
- Research Institution
  Tokyo Institute of Technology
  National Institute for Basic Biology
Study on Genetic Recombination Systems in Eukaryotes
- Principal Investigator
  
  OGAWA Hideyuki
- Project Period (FY)
  1994 – 1998
- Research Category
  
  Grant-in-Aid for Specially Promoted Research
- Review Section
  
  Biological Sciences
- Research Institution
  Iwate College of Nursing
  Osaka University

All 2023 2022 2021 2020 2018 2017

All Journal Article Presentation

[Journal Article] Hop2-Mnd1 and Swi5-Sfr1 stimulate Dmc1 filament assembly using distinct mechanisms2023
- Author(s)
  Lee Wei、Iwasaki Hiroshi、Tsubouchi Hideo、Li Hung-Wen
- Journal Title
  
  Nucleic Acids Research
  
  Volume: 51 Issue: 16 Pages: 8550-8562
- DOI
  10.1093/nar/gkad561
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-22H00404, KAKENHI-PROJECT-23K27102
[Journal Article] Analysis of the indispensable RAD51 cofactor BRCA2 in<i>Naganishia liquefaciens</i>, a Basidiomycota yeast2023
- Author(s)
  Palihati Maierdan、Iwasaki Hiroshi、Tsubouchi Hideo
- Journal Title
  
  Life Science Alliance
  
  Volume: 7 Issue: 2 Pages: e202302342-e202302342
- DOI
  10.26508/lsa.202302342
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-22H00404, KAKENHI-PROJECT-23K27102
[Journal Article] The Hop2-Mnd1 Complex and Its Regulation of Homologous Recombination2023
- Author(s)
  Tsubouchi Hideo
- Journal Title
  
  Biomolecules
  
  Volume: 13 Issue: 4 Pages: 662-662
- DOI
  10.3390/biom13040662
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23K27102
[Journal Article] Euchromatin factors HULC and Set1C affect heterochromatin organization and mating-type switching in fission yeast <i>Schizosaccharomyces pombe</i>2022
- Author(s)
  Esquivel-Chavez Alfredo、Maki Takahisa、Tsubouchi Hideo、Handa Testuya、Kimura Hiroshi、Haber James E.、Thon Genevieve、Iwasaki Hiroshi
- Journal Title
  
  Genes Genet. Syst.
  
  Volume: 97 Issue: 3 Pages: 123-138
- DOI
  10.1266/ggs.22-00012
- ISSN
  1341-7568, 1880-5779
- Year and Date
  2022-06-01
- Language
  English
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18H02371, KAKENHI-PLANNED-18H05527, KAKENHI-PROJECT-22H00404
[Journal Article] A conserved Ctp1/CtIP C-terminal peptide stimulates Mre11 endonuclease activity2021
- Author(s)
  Zdravkovic Aleksandar、Daley James M.、Dutta Arijit、Niwa Tatsuya、Murayama Yasuto、Kanamaru Shuji、Ito Kentaro、Maki Takahisa、Argunhan Bilge、Takahashi Masayuki、Tsubouchi Hideo、Sung Patrick、Iwasaki Hiroshi
- Journal Title
  
  Proceedings of the National Academy of Sciences
  
  Volume: 118 Issue: 11 Pages: 1-7
- DOI
  10.1073/pnas.2016287118
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18H02371, KAKENHI-PROJECT-19H03160, KAKENHI-PROJECT-19K16039, KAKENHI-PROJECT-20K15713, KAKENHI-PROJECT-18K05314, KAKENHI-PROJECT-21K05268, KAKENHI-PROJECT-18H03985
[Journal Article] Post-translational modification of factors involved in homologous recombination2021
- Author(s)
  Argunhan Bilge、Iwasaki Hiroshi、Tsubouchi Hideo
- Journal Title
  
  DNA Repair
  
  Volume: 104 Pages: 103114-103114
- DOI
  10.1016/j.dnarep.2021.103114
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18H03985, KAKENHI-PROJECT-18H02371
[Journal Article] Rrp1 translocase and ubiquitin ligase activities restrict the genome destabilising effects of Rad51 in fission yeast2021
- Author(s)
  Muraszko Jakub、Kramarz Karol、Argunhan Bilge、Ito Kentaro、Baranowska Gabriela、Kurokawa Yumiko、Murayama Yasuto、Tsubouchi Hideo、Lambert Sarah、Iwasaki Hiroshi、Dziadkowiec Dorota
- Journal Title
  
  Nucleic Acids Research
  
  Volume: 49 Issue: 12 Pages: 6832-6848
- DOI
  10.1093/nar/gkab511
- NAID
  120007124423
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19H03160, KAKENHI-PROJECT-18H03985, KAKENHI-PROJECT-18H02371
[Journal Article] A novel motif of Rad51 serves as an interaction hub for recombination auxiliary factors2021
- Author(s)
  Afshar Negar、Argunhan Bilge、Palihati Maierdan、Taniguchi Goki、Tsubouchi Hideo、Iwasaki Hiroshi
- Journal Title
  
  eLife
  
  Volume: 10
- DOI
  10.7554/elife.64131
- NAID
  120007124436
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18H02371, KAKENHI-PROJECT-20K15713, KAKENHI-PROJECT-18H03985
[Journal Article] Homology length dictates the requirement for Rad51 and Rad52 in gene targeting in the Basidiomycota yeast Naganishia liquefaciens2021
- Author(s)
  Palihati Maierdan、Tsubouchi Hideo、Argunhan Bilge、Kajitani Rei、Bakenova Omirgul、Han Yong-Woon、Murayama Yasuto、Itoh Takehiko、Iwasaki Hiroshi
- Journal Title
  
  Current Genetics
  
  Volume: 67 Issue: 6 Pages: 919-936
- DOI
  10.1007/s00294-021-01201-3
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-19H03160, KAKENHI-PROJECT-18H03985, KAKENHI-PROJECT-18H02371
[Journal Article] Biochemical properties of fission yeast homologous recombination enzymes2021
- Author(s)
  Tsubouchi Hideo、Argunhan Bilge、Iwasaki Hiroshi
- Journal Title
  
  Current Opinion in Genetics & Development
  
  Volume: 71 Pages: 19-26
- DOI
  10.1016/j.gde.2021.06.006
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18H03985, KAKENHI-PROJECT-18H02371
[Journal Article] Cooperative interactions facilitate stimulation of Rad51 by the Swi5-Sfr1 auxiliary factor complex.2020
- Author(s)
  Argunhan B, Sakakura M, Afshar N, Kurihara M, Ito K, Maki T, Kanamaru S, Murayama Y, Tsubouchi H, Takahashi M, Takahashi H, Iwasaki H.
- Journal Title
  
  eLife
  
  Volume: 9
- DOI
  10.7554/elife.52566
- NAID
  120007117502
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18K05314, KAKENHI-PROJECT-19K16039, KAKENHI-PLANNED-15H05974, KAKENHI-PUBLICLY-18H04626, KAKENHI-PLANNED-18H05426, KAKENHI-PROJECT-18H03985, KAKENHI-PROJECT-18H02393, KAKENHI-PROJECT-19H03160, KAKENHI-PROJECT-17K15061, KAKENHI-PROJECT-18H02371, KAKENHI-PROJECT-20K15713
[Journal Article] Two Auxiliary Factors Promote Dmc1-Driven DNA Strand Exchange via Stepwise Mechanisms2020
- Author(s)
  Hideo Tsubouchi1, Bilge Argunhan, Kentaro Ito, Masayuki Takahashi, Hiroshi Iwasaki
- Journal Title
  
  PNAS
  
  Volume: -
- NAID
  120007117440
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-18H02371
[Journal Article] Draft Genome Sequence of Naganishia liquefaciens Strain N6, Isolated from the Japan Trench2020
- Author(s)
  Han Yong-Woon、Kajitani Rei、Morimoto Hiroya、Palihati Maierdan、Kurokawa Yumiko、Ryusui Rie、Argunhan Bilge、Tsubouchi Hideo、Abe Fumiyoshi、Kajiwara Susumu、Iwasaki Hiroshi、Itoh Takehiko
- Journal Title
  
  Microbiology Resource Announcements
  
  Volume: 9 Issue: 47
- DOI
  10.1128/mra.00827-20
- NAID
  120007124437
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18H02371, KAKENHI-PROJECT-18K05397, KAKENHI-PROJECT-18H03985, KAKENHI-PROJECT-19H03206, KAKENHI-PROJECT-20K15713
[Journal Article] Polo is not solo in meiosis2018
- Author(s)
  Argunhan Bilge、Tsubouchi Tomomi、Tsubouchi Hideo
- Journal Title
  
  Cell Cycle
  
  Volume: (17) 3 Issue: 3 Pages: 1-2
- DOI
  10.1080/15384101.2017.1411435
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16H07422
[Journal Article] Fundamental cell cycle kinases collaborate to ensure timely destruction of the synaptonemal complex during meiosis.2017
- Author(s)
  Argunhan B, Leung WK, Afshar N, Terentyev Y, Subramanian VV, Murayama Y, Hochwagen A, Iwasaki H, Tsubouchi T, Tsubouchi H.
- Journal Title
  
  EMBO J
  
  Volume: 36 Issue: 17 Pages: 2488-2509
- DOI
  10.15252/embj.201695895
- NAID
  120007117670
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-INTERNATIONAL-15K21761, KAKENHI-PUBLICLY-16H01404, KAKENHI-PROJECT-16H06160, KAKENHI-PROJECT-16H07422, KAKENHI-PLANNED-15H05974
[Journal Article] Exiting prophase I: no clear boundary2017
- Author(s)
  Tsubouchi Hideo、Argunhan Bilge、Tsubouchi Tomomi
- Journal Title
  
  Current Genetics
  
  Volume: 64 Issue: 2 Pages: 423-427
- DOI
  10.1007/s00294-017-0771-y
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16H07422
[Presentation] Post-Replicative Accumulation of Rad51 Is Resolved by Rad54 in S. pombe2023
- Author(s)
  Goki Taniguchi, Hiroshi Iwasaki, Hideo Tsubouchi
- Organizer
  Cold Spring Harbor Asia Conference
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K27102
[Presentation] Exploiting Basidiomycota Yeast as a Model to Study BRCA22023
- Author(s)
  Maierdan Palihati, Hiroshi Iwasaki, Hideo Tsubouchi
- Organizer
  第４６回日本分子生物学会年会・神戸
- Data Source
  KAKENHI-PROJECT-23K27102
[Presentation] BRCA2とRad52の機能的相違：ナガニシア酵母をモデルとして2022
- Author(s)
  坪内英生、Palihati Maierdan、岩﨑博史
- Organizer
  日本遺伝学会第９４回大会
- Data Source
  KAKENHI-PROJECT-18H02371
[Presentation] BRCA2 を持つ担子菌 Naganishia liquefaciens （ナガニシア酵母）を用いた相同組換え機構の解析2021
- Author(s)
  坪内英生、Palihati Maierdan、青木陸登、岩﨑博史
- Organizer
  日本遺伝学会第９３回大会
- Data Source
  KAKENHI-PROJECT-18H02371
[Presentation] BRCA2 を持つ担子菌 Naganishia liquefaciens（ナガニシア酵母）を用いた相同組換え機構の生化学的理解2021
- Author(s)
  坪内英生、Palihati Maierdan、青木陸登、岩﨑博史
- Organizer
  第５４回酵母遺伝学フォーラム
- Data Source
  KAKENHI-PROJECT-18H02371
[Presentation] クリプトコッカス酵母をモデルとした相同組換え研究の新展開2020
- Author(s)
  坪内英生
- Organizer
  日本遺伝学会第91回大会
- Invited
- Data Source
  KAKENHI-PROJECT-18H02371
[Presentation] BRCA2を持つ担子菌Naganishia liquefaciens(ナガニシア酵母)を用いた相同組換え機構の解析2020
- Author(s)
  坪内英生、Palihati Maierdan、Bakenova Omirgul、青木陸登、今井健人、梶谷嶺、韓龍雲、伊藤武彦、岩﨑博史
- Organizer
  酵母遺伝学フォーラム　第 53 回研究報告会
- Data Source
  KAKENHI-PROJECT-18H02371
[Presentation] 減数分裂期特異的に形成される高次染色体構造を解消するメカニズム2017
- Author(s)
  坪内英生
- Organizer
  第24回DNA複製・組換え・修復ワークショップ
- Data Source
  KAKENHI-PROJECT-16H07422

1. OGAWA Hideyuki (70028207)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
2. SHINOHARA Akira (00252578)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
3. SHIBATA Takehiko (70087550)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
4. OGAWA Tomoko (80028208)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
5. ARGUNHAN Bilge (30792759)

# of Collaborated Projects: 1 results

# of Collaborated Products: 2 results
6. TSUBOUCHI Tomomi (70754505)

# of Collaborated Projects: 1 results

# of Collaborated Products: 3 results
7. MURAYAMA Yasuto

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
8. 金丸周司

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
9. 岩崎博史

# of Collaborated Projects: 0 results

# of Collaborated Products: 5 results

TSUBOUCHI HIDEO 坪内 英生

TSUBOUCHI Hideo 坪内 英生

Research Projects

Research Products

Co-Researchers

ナガニシア酵母で解き明かす相同組換え制御機構Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Similarity and difference between Rad51 and Dmc1, two RecA homologsPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Understanding the mechanisms for ensuring timely destruction of the synaptonemal complex during meiosisPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Study on Genetic Recombination Systems in Eukaryotes

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

[Journal Article] Hop2-Mnd1 and Swi5-Sfr1 stimulate Dmc1 filament assembly using distinct mechanisms2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Analysis of the indispensable RAD51 cofactor BRCA2 in<i>Naganishia liquefaciens</i>, a Basidiomycota yeast2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] The Hop2-Mnd1 Complex and Its Regulation of Homologous Recombination2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Euchromatin factors HULC and Set1C affect heterochromatin organization and mating-type switching in fission yeast <i>Schizosaccharomyces pombe</i>2022

Author(s)

Journal Title

DOI

ISSN

Year and Date

Language

Data Source

[Journal Article] A conserved Ctp1/CtIP C-terminal peptide stimulates Mre11 endonuclease activity2021

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Post-translational modification of factors involved in homologous recombination2021

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Rrp1 translocase and ubiquitin ligase activities restrict the genome destabilising effects of Rad51 in fission yeast2021

Author(s)

Journal Title

DOI

NAID

Data Source

[Journal Article] A novel motif of Rad51 serves as an interaction hub for recombination auxiliary factors2021

Author(s)

Journal Title

DOI

NAID

Data Source

[Journal Article] Homology length dictates the requirement for Rad51 and Rad52 in gene targeting in the Basidiomycota yeast Naganishia liquefaciens2021

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Biochemical properties of fission yeast homologous recombination enzymes2021

TSUBOUCHI HIDEO 坪内英生

TSUBOUCHI Hideo 坪内英生

[Presentation] BRCA2を持つ担子菌Naganishia liquefaciens(ナガニシア酵母)を用いた相同組換え機構の解析2020