Miyazaki Ryoji 宮崎亮次

Researcher Number	30827564
Other IDs
Affiliation (Current)	2025: 奈良先端科学技術大学院大学, 先端科学技術研究科, 助教
Affiliation (based on the past Project Information) *help	2020 – 2025: 奈良先端科学技術大学院大学, 先端科学技術研究科, 助教 2018 – 2019: 京都大学, ウイルス・再生医科学研究所, 特定研究員
Review Section/Research Field	Principal Investigator Basic Section 43030:Functional biochemistry-related / Transformative Research Areas, Section (III) / Basic Section 43010:Molecular biology-related / 0701:Biology at molecular to cellular levels, and related fields Except Principal Investigator Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
Keywords	Principal Investigator in vivo光架橋法 / AlphaFold 2 / YfgM / PpiD / Secトランスロコン / VemP / 新生ポリペプチド鎖 / 翻訳途上ポリペプチド鎖 / 翻訳停止配列 / 大腸菌 … More / DsbA / YeeD / YeeE / チオ硫酸イオン / 硫黄 / in vivo光架橋解析 / X線結晶構造解析 / 硫黄転移酵素 / チオ硫酸トランスポーター / 翻訳伸長停止 / 部位特異的in vivo光架橋法 / 細胞内生化学 / フォールディング / タンパク質相互作用 … More Except Principal Investigator BAM複合体 / 中性子反射率法 / グラム陰性菌 / TOM複合体 / タンパク質輸送装置 / マグネットソーム / ベータバレル / 外膜 / マグネトソーム / 膜タンパク質 / 磁性細菌 / 大腸菌 Less

Analysis of super complex of protein translocators using multiple biophysical approaches
- Principal Investigator
  
  塩田拓也
- Project Period (FY)
  2024 – 2026
- Research Category
  
  Fund for the Promotion of Joint International Research (International Collaborative Research)
- Review Section
  
  Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
- Research Institution
  University of Miyazaki
高度化した光架橋法による細菌外膜タンパク質の細胞内成熟動態の解明Principal Investigator
- Principal Investigator
  
  宮崎亮次
- Project Period (FY)
  2024 – 2027
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 43030:Functional biochemistry-related
- Research Institution
  Nara Institute of Science and Technology
特殊な硫黄間相互作用が要となる新奇チオ硫酸トランスポーターの解析Principal Investigator
- Principal Investigator
  
  宮崎亮次
- Project Period (FY)
  2022 – 2023
- Research Category
  
  Grant-in-Aid for Transformative Research Areas (A)
- Review Section
  
  Transformative Research Areas, Section (III)
- Research Institution
  Nara Institute of Science and Technology
生細胞内での膜透過途上の新生ポリペプチド鎖の相互作用動態解析Principal Investigator
- Principal Investigator
  
  宮崎亮次
- Project Period (FY)
  2022 – 2024
- Research Category
  
  Grant-in-Aid for Early-Career Scientists
- Review Section
  
  Basic Section 43030:Functional biochemistry-related
- Research Institution
  Nara Institute of Science and Technology
Optimization of multiple method baced on neutron reflectometry for analysis of biogenesis of Gram-negative bacterial membrane.
- Principal Investigator
  
  Shiota Takuya
- Project Period (FY)
  2021 – 2023
- Research Category
  
  Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
- Review Section
  
  Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
- Research Institution
  University of Miyazaki
In vivo photo-crosslinking analysis of dynamic interactions of a nascent polypeptide in a living cellPrincipal Investigator
- Principal Investigator
  
  Miyazaki Ryoji
- Project Period (FY)
  2020 – 2022
- Research Category
  
  Grant-in-Aid for Early-Career Scientists
- Review Section
  
  Basic Section 43010:Molecular biology-related
- Research Institution
  Nara Institute of Science and Technology
Analysis of dynamic interaction and folding process of a nascent polypeptide in living cellsPrincipal Investigator
- Principal Investigator
  
  Miyazaki Ryoji
- Project Period (FY)
  2018 – 2019
- Research Category
  
  Grant-in-Aid for Research Activity Start-up
- Review Section
  
  0701:Biology at molecular to cellular levels, and related fields
- Research Institution
  Kyoto University

All 2024 2023 2022 2021 2020 2019 2018

All Journal Article Presentation

[Journal Article] YeeD is an essential partner for YeeE-mediated thiosulfate uptake in bacteria and regulates thiosulfate ion decomposition2024
- Author(s)
  Ikei Mai、Miyazaki Ryoji、Monden Keigo、Naito Yusuke、Takeuchi Azusa、Takahashi Yutaro S.、Tanaka Yoshiki、Murata Keina、Mori Takaharu、Ichikawa Muneyoshi、Tsukazaki Tomoya
- Journal Title
  
  PLOS Biology
  
  Volume: 22 Issue: 4 Pages: e3002601-e3002601
- DOI
  10.1371/journal.pbio.3002601
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-22K15061, KAKENHI-PROJECT-21KK0125, KAKENHI-PROJECT-23K23850, KAKENHI-PLANNED-21H05155, KAKENHI-PLANNED-21H05157
[Journal Article] Analyzing protein intermediate interactions in living E.?coli cells using site-specific photo-crosslinking combined with chemical crosslinking2023
- Author(s)
  Miyazaki Ryoji、Akiyama Yoshinori
- Journal Title
  
  STAR Protocols
  
  Volume: 4 Issue: 2 Pages: 102178-102178
- DOI
  10.1016/j.xpro.2023.102178
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-22K15061, KAKENHI-PROJECT-20K15715, KAKENHI-PROJECT-21KK0126, KAKENHI-PUBLICLY-22H05567, KAKENHI-PROJECT-23K23835
[Journal Article] A Photo-Crosslinking Approach to Monitoring the Assembly of an LptD Intermediate with LptE in a Living Cell2022
- Author(s)
  Miyazaki Ryoji、Mori Hiroyuki、Akiyama Yoshinori
- Journal Title
  
  Methods in Molecular Biology: Lipopolysaccharide Transport
  
  Volume: 2548 Pages: 97-107
- DOI
  10.1007/978-1-0716-2581-1_7
- ISBN
  9781071625804, 9781071625811
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-22K15061, KAKENHI-PROJECT-21KK0126, KAKENHI-PROJECT-20K15715, KAKENHI-PROJECT-23K23835
[Journal Article] Inner membrane YfgM-PpiD heterodimer acts as a functional unit that associates with the SecY/E/G translocon and promotes protein translocation2022
- Author(s)
  Miyazaki Ryoji、Ai Mengting、Tanaka Natsuko、Suzuki Takehiro、Dhomae Naoshi、Tsukazaki Tomoya、Akiyama Yoshinori、Mori Hiroyuki
- Journal Title
  
  Journal of Biological Chemistry
  
  Volume: 298 Issue: 11 Pages: 102572-102572
- DOI
  10.1016/j.jbc.2022.102572
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-22K15061, KAKENHI-PROJECT-21KK0125, KAKENHI-PROJECT-21KK0126, KAKENHI-PLANNED-21H05155, KAKENHI-PROJECT-20K06556, KAKENHI-PROJECT-21K19226, KAKENHI-PROJECT-20K15715, KAKENHI-PROJECT-23K23835, KAKENHI-PUBLICLY-22H05567, KAKENHI-PROJECT-23K23850
[Journal Article] Edge-strand of BepA interacts with immature LptD on the β-barrel assembly machine to direct it to on- and off-pathways2021
- Author(s)
  Miyazaki Ryoji、Watanabe Tetsuro、Yoshitani Kohei、Akiyama Yoshinori
- Journal Title
  
  eLife
  
  Volume: 10
- DOI
  10.7554/elife.70541
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-20K15715
[Journal Article] PiXie Analysis for Monitoring Dynamic Protein Interaction and Folding in a Living Cell2021
- Author(s)
  MIYAZAKI Ryoji、MORI Hiroyuki、AKIYAMA Yoshinori
- Journal Title
  
  Seibutsu Butsuri
  
  Volume: 61 Issue: 1 Pages: 036-039
- DOI
  10.2142/biophys.61.036
- NAID
  130007976685
- ISSN
  0582-4052, 1347-4219
- Language
  Japanese
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20K15715
[Journal Article] Reversible auto-inhibitory regulation of Escherichia coli metallopeptidase BepA for selective β-barrel protein degradation.2020
- Author(s)
  Daimon 他7名
- Journal Title
  
  Proc. Natl. Acad. Sci. USA.
  
  Volume: 117 Issue: 45 Pages: 27989-27996
- DOI
  10.1073/pnas.2010301117
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18K05396, KAKENHI-PROJECT-19K06562, KAKENHI-PROJECT-20K06556, KAKENHI-PROJECT-20K15715, KAKENHI-PROJECT-18H02386, KAKENHI-PROJECT-18H02404, KAKENHI-PROJECT-17K07334, KAKENHI-PROJECT-18K06136, KAKENHI-PROJECT-19K22395, KAKENHI-PROJECT-18KK0197, KAKENHI-PROJECT-18H02405
[Journal Article] Fine interaction profiling of VemP and mechanisms responsible for its translocation-coupled arrest-cancelation2020
- Author(s)
  Miyazaki Ryoji、Akiyama Yoshinori、Mori Hiroyuki
- Journal Title
  
  eLife
  
  Volume: 9
- DOI
  10.7554/elife.62623
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-20K06556, KAKENHI-PROJECT-20K15715, KAKENHI-PROJECT-18H02404, KAKENHI-PROJECT-17K07334
[Journal Article] A photo-cross-linking approach to monitor protein dynamics in living cells2019
- Author(s)
  Miyazaki Ryoji、Akiyama Yoshinori、Mori Hiroyuki
- Journal Title
  
  Biochimica et Biophysica Acta (BBA) - General Subjects
  
  Volume: 印刷中 Issue: 2 Pages: 129317-129317
- DOI
  10.1016/j.bbagen.2019.03.003
- Peer Reviewed
- Data Source
  KAKENHI-PUBLICLY-17H05666, KAKENHI-PUBLICLY-17H05879, KAKENHI-PROJECT-19K21179, KAKENHI-PROJECT-20K06556, KAKENHI-PROJECT-17K07334
[Presentation] PpiD/YfgM複合体はSec複合体とDsbAを連結する2023
- Author(s)
  宮崎亮次
- Organizer
  2022年度国立遺伝学研究所研究会「単細胞生物に見られる生体プロセスの恒常性維持システム」
- Invited
- Data Source
  KAKENHI-PROJECT-20K15715
[Presentation] PpiD/YfgM複合体はSec複合体とDsbAを連結する2023
- Author(s)
  宮崎亮次
- Organizer
  2022年度国立遺伝学研究所研究会「単細胞生物に見られる生体プロセスの恒常性維持システム」
- Invited
- Data Source
  KAKENHI-PROJECT-22K15061
[Presentation] 内膜タンパク質PpiDはSecトランスロコンとDsbAを連結し、膜透過基質タンパク質へのジスルフィド結合導入を助ける2023
- Author(s)
  宮崎亮次、鈴木健裕、堂前直、塚崎智也
- Organizer
  第96回日本生化学会大会
- Data Source
  KAKENHI-PROJECT-22K15061
[Presentation] タンパク質膜透過・輸送に関わるPpiD/YfgM複合体の in vivo 光架橋解析2022
- Author(s)
  宮崎亮次、鈴木健裕、堂前直、塚崎智也
- Organizer
  第22回日本蛋白質科学会年会
- Invited
- Data Source
  KAKENHI-PROJECT-22K15061
[Presentation] in vivo光架橋法によるタンパク質膜透過関連因子PpiDの相互作用解析2022
- Author(s)
  宮崎亮次、鈴木健裕、堂前直、塚崎智也
- Organizer
  第18回 21世紀大腸菌研究会
- Data Source
  KAKENHI-PROJECT-20K15715
[Presentation] in vivo光架橋法による内膜タンパク質複合体PpiD/YfgMの機能解析2022
- Author(s)
  宮崎亮次
- Organizer
  2021年度国立遺伝学研究所研究会「単細胞システムの複製と維持における生体高分子の機能」
- Invited
- Data Source
  KAKENHI-PROJECT-20K15715
[Presentation] PpiD/YfgM複合体はSec複合体とDsbAを連結する2022
- Author(s)
  宮崎亮次
- Organizer
  2022年度国立遺伝学研究所研究会「単細胞生物に見られる生体プロセスの恒常性維持システム」
- Invited
- Data Source
  KAKENHI-PROJECT-21KK0126
[Presentation] タンパク質膜透過・輸送に関わるPpiD/YfgM複合体の in vivo 光架橋解析2022
- Author(s)
  宮崎亮次、鈴木健裕、堂前直、塚崎智也
- Organizer
  第22回日本蛋白質科学会年会
- Invited
- Data Source
  KAKENHI-PROJECT-20K15715
[Presentation] in vivo光架橋法による内膜タンパク質複合体PpiD/YfgMの機能解析2022
- Author(s)
  宮崎亮次
- Organizer
  2021年度国立遺伝学研究所研究会「単細胞システムの複製と維持における生体高分子の機能」
- Data Source
  KAKENHI-PROJECT-21KK0126
[Presentation] Structural and functional analysis of a thiosulfate transporter complex2022
- Author(s)
  Ikei Mai、Takeuchi Azusa、Naito Yusuke、Ichikawa Muneyoshi、Miyazaki Ryoji、Tsukazaki Tomoya
- Organizer
  The 22nd Annual Scientific Meeting of the Nitric Oxide Society of Japan
- Int'l Joint Research
- Data Source
  KAKENHI-PUBLICLY-22H05567
[Presentation] タンパク質膜透過・輸送に関わるPpiD/YfgM複合体の in vivo 光架橋解析2022
- Author(s)
  宮崎亮次、鈴木健裕、堂前直、塚崎智也
- Organizer
  第22回日本蛋白質科学会年会
- Data Source
  KAKENHI-PROJECT-21KK0126
[Presentation] in vivo光架橋法によるタンパク質膜透過関連因子PpiDの相互作用解析2022
- Author(s)
  宮崎亮次、鈴木健裕、堂前直、塚崎智也
- Organizer
  第18回 21世紀大腸菌研究会
- Data Source
  KAKENHI-PROJECT-22K15061
[Presentation] in vivo光架橋法によるタンパク質膜透過関連因子PpiDの相互作用解析2022
- Author(s)
  宮崎亮次、鈴木健裕、堂前直、塚崎智也
- Organizer
  第18回 21世紀大腸菌研究会
- Data Source
  KAKENHI-PROJECT-21KK0126
[Presentation] 改良型部位特異的in vivo光架橋法による生細胞内での翻訳途上ポリペプチド鎖の相互作用動態解析2019
- Author(s)
  宮崎亮次、秋山芳展、森博幸
- Organizer
  第92回日本生化学会大会
- Data Source
  KAKENHI-PROJECT-19K21179
[Presentation] Analysis of cis and trans factors involved in export and translation arrest of VemP by an in vivo photo cross-linking approach2018
- Author(s)
  Ryoji Miyazaki, Yoshinori Akiyama, Hiroyuki Mori
- Organizer
  International symposium Proteins: From the Cradle to the Grave
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K21179

1. Shiota Takuya (20819304)

# of Collaborated Projects: 2 results

# of Collaborated Products: 0 results
2. 田岡東 (20401888)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
3. 竹田弘法 (80816588)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
4. 荒磯裕平 (20753726)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
5. 青木英莉子 (80822874)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
6. 森博幸

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results

Miyazaki Ryoji 宮崎 亮次

Research Projects

Research Products

Co-Researchers

Analysis of super complex of protein translocators using multiple biophysical approaches

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

高度化した光架橋法による細菌外膜タンパク質の細胞内成熟動態の解明Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

特殊な硫黄間相互作用が要となる新奇チオ硫酸トランスポーターの解析Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

生細胞内での膜透過途上の新生ポリペプチド鎖の相互作用動態解析Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Optimization of multiple method baced on neutron reflectometry for analysis of biogenesis of Gram-negative bacterial membrane.

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

In vivo photo-crosslinking analysis of dynamic interactions of a nascent polypeptide in a living cellPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Analysis of dynamic interaction and folding process of a nascent polypeptide in living cellsPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

[Journal Article] YeeD is an essential partner for YeeE-mediated thiosulfate uptake in bacteria and regulates thiosulfate ion decomposition2024

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Analyzing protein intermediate interactions in living E.?coli cells using site-specific photo-crosslinking combined with chemical crosslinking2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] A Photo-Crosslinking Approach to Monitoring the Assembly of an LptD Intermediate with LptE in a Living Cell2022

Author(s)

Journal Title

DOI

ISBN

Data Source

[Journal Article] Inner membrane YfgM-PpiD heterodimer acts as a functional unit that associates with the SecY/E/G translocon and promotes protein translocation2022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Edge-strand of BepA interacts with immature LptD on the β-barrel assembly machine to direct it to on- and off-pathways2021

Author(s)

Journal Title

DOI

Data Source

[Journal Article] PiXie Analysis for Monitoring Dynamic Protein Interaction and Folding in a Living Cell2021

Author(s)

Journal Title

DOI

NAID

ISSN

Language

Data Source

Miyazaki Ryoji 宮崎亮次