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Miyazaki Ryoji  宮崎 亮次

ORCIDConnect your ORCID iD *help
Researcher Number 30827564
Other IDs
Affiliation (Current) 2025: 奈良先端科学技術大学院大学, 先端科学技術研究科, 助教
Affiliation (based on the past Project Information) *help 2020 – 2025: 奈良先端科学技術大学院大学, 先端科学技術研究科, 助教
2018 – 2019: 京都大学, ウイルス・再生医科学研究所, 特定研究員
Review Section/Research Field
Principal Investigator
Basic Section 43030:Functional biochemistry-related / Transformative Research Areas, Section (III) / Basic Section 43010:Molecular biology-related / 0701:Biology at molecular to cellular levels, and related fields
Except Principal Investigator
Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
Keywords
Principal Investigator
in vivo光架橋法 / AlphaFold 2 / YfgM / PpiD / Secトランスロコン / VemP / 新生ポリペプチド鎖 / 翻訳途上ポリペプチド鎖 / 翻訳停止配列 / 大腸菌 … More / DsbA / YeeD / YeeE / チオ硫酸イオン / 硫黄 / in vivo光架橋解析 / X線結晶構造解析 / 硫黄転移酵素 / チオ硫酸トランスポーター / 翻訳伸長停止 / 部位特異的in vivo光架橋法 / 細胞内生化学 / フォールディング / タンパク質相互作用 … More
Except Principal Investigator
BAM複合体 / 中性子反射率法 / グラム陰性菌 / TOM複合体 / タンパク質輸送装置 / マグネットソーム / ベータバレル / 外膜 / マグネトソーム / 膜タンパク質 / 磁性細菌 / 大腸菌 Less
  • Research Projects

    (7 results)
  • Research Products

    (24 results)
  • Co-Researchers

    (6 People)
  •  Analysis of super complex of protein translocators using multiple biophysical approaches

    • Principal Investigator
      塩田 拓也
    • Project Period (FY)
      2024 – 2026
    • Research Category
      Fund for the Promotion of Joint International Research (International Collaborative Research)
    • Review Section
      Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
    • Research Institution
      University of Miyazaki
  •  高度化した光架橋法による細菌外膜タンパク質の細胞内成熟動態の解明Principal Investigator

    • Principal Investigator
      宮崎 亮次
    • Project Period (FY)
      2024 – 2027
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Review Section
      Basic Section 43030:Functional biochemistry-related
    • Research Institution
      Nara Institute of Science and Technology
  •  特殊な硫黄間相互作用が要となる新奇チオ硫酸トランスポーターの解析Principal Investigator

    • Principal Investigator
      宮崎 亮次
    • Project Period (FY)
      2022 – 2023
    • Research Category
      Grant-in-Aid for Transformative Research Areas (A)
    • Review Section
      Transformative Research Areas, Section (III)
    • Research Institution
      Nara Institute of Science and Technology
  •  生細胞内での膜透過途上の新生ポリペプチド鎖の相互作用動態解析Principal Investigator

    • Principal Investigator
      宮崎 亮次
    • Project Period (FY)
      2022 – 2024
    • Research Category
      Grant-in-Aid for Early-Career Scientists
    • Review Section
      Basic Section 43030:Functional biochemistry-related
    • Research Institution
      Nara Institute of Science and Technology
  •  Optimization of multiple method baced on neutron reflectometry for analysis of biogenesis of Gram-negative bacterial membrane.

    • Principal Investigator
      Shiota Takuya
    • Project Period (FY)
      2021 – 2023
    • Research Category
      Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
    • Review Section
      Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
    • Research Institution
      University of Miyazaki
  •  In vivo photo-crosslinking analysis of dynamic interactions of a nascent polypeptide in a living cellPrincipal Investigator

    • Principal Investigator
      Miyazaki Ryoji
    • Project Period (FY)
      2020 – 2022
    • Research Category
      Grant-in-Aid for Early-Career Scientists
    • Review Section
      Basic Section 43010:Molecular biology-related
    • Research Institution
      Nara Institute of Science and Technology
  •  Analysis of dynamic interaction and folding process of a nascent polypeptide in living cellsPrincipal Investigator

    • Principal Investigator
      Miyazaki Ryoji
    • Project Period (FY)
      2018 – 2019
    • Research Category
      Grant-in-Aid for Research Activity Start-up
    • Review Section
      0701:Biology at molecular to cellular levels, and related fields
    • Research Institution
      Kyoto University

All 2024 2023 2022 2021 2020 2019 2018

All Journal Article Presentation

  • [Journal Article] YeeD is an essential partner for YeeE-mediated thiosulfate uptake in bacteria and regulates thiosulfate ion decomposition2024

    • Author(s)
      Ikei Mai、Miyazaki Ryoji、Monden Keigo、Naito Yusuke、Takeuchi Azusa、Takahashi Yutaro S.、Tanaka Yoshiki、Murata Keina、Mori Takaharu、Ichikawa Muneyoshi、Tsukazaki Tomoya
    • Journal Title

      PLOS Biology

      Volume: 22 Issue: 4 Pages: e3002601-e3002601

    • DOI

      10.1371/journal.pbio.3002601

    • Peer Reviewed / Open Access / Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-22K15061, KAKENHI-PROJECT-21KK0125, KAKENHI-PROJECT-23K23850, KAKENHI-PLANNED-21H05155, KAKENHI-PLANNED-21H05157
  • [Journal Article] Analyzing protein intermediate interactions in living E.?coli cells using site-specific photo-crosslinking combined with chemical crosslinking2023

    • Author(s)
      Miyazaki Ryoji、Akiyama Yoshinori
    • Journal Title

      STAR Protocols

      Volume: 4 Issue: 2 Pages: 102178-102178

    • DOI

      10.1016/j.xpro.2023.102178

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-22K15061, KAKENHI-PROJECT-20K15715, KAKENHI-PROJECT-21KK0126, KAKENHI-PUBLICLY-22H05567, KAKENHI-PROJECT-23K23835
  • [Journal Article] A Photo-Crosslinking Approach to Monitoring the Assembly of an LptD Intermediate with LptE in a Living Cell2022

    • Author(s)
      Miyazaki Ryoji、Mori Hiroyuki、Akiyama Yoshinori
    • Journal Title

      Methods in Molecular Biology: Lipopolysaccharide Transport

      Volume: 2548 Pages: 97-107

    • DOI

      10.1007/978-1-0716-2581-1_7

    • ISBN
      9781071625804, 9781071625811
    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-22K15061, KAKENHI-PROJECT-21KK0126, KAKENHI-PROJECT-20K15715, KAKENHI-PROJECT-23K23835
  • [Journal Article] Inner membrane YfgM-PpiD heterodimer acts as a functional unit that associates with the SecY/E/G translocon and promotes protein translocation2022

    • Author(s)
      Miyazaki Ryoji、Ai Mengting、Tanaka Natsuko、Suzuki Takehiro、Dhomae Naoshi、Tsukazaki Tomoya、Akiyama Yoshinori、Mori Hiroyuki
    • Journal Title

      Journal of Biological Chemistry

      Volume: 298 Issue: 11 Pages: 102572-102572

    • DOI

      10.1016/j.jbc.2022.102572

    • Peer Reviewed / Open Access / Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-22K15061, KAKENHI-PROJECT-21KK0125, KAKENHI-PROJECT-21KK0126, KAKENHI-PLANNED-21H05155, KAKENHI-PROJECT-20K06556, KAKENHI-PROJECT-21K19226, KAKENHI-PROJECT-20K15715, KAKENHI-PROJECT-23K23835, KAKENHI-PUBLICLY-22H05567, KAKENHI-PROJECT-23K23850
  • [Journal Article] Edge-strand of BepA interacts with immature LptD on the β-barrel assembly machine to direct it to on- and off-pathways2021

    • Author(s)
      Miyazaki Ryoji、Watanabe Tetsuro、Yoshitani Kohei、Akiyama Yoshinori
    • Journal Title

      eLife

      Volume: 10

    • DOI

      10.7554/elife.70541

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-20K15715
  • [Journal Article] PiXie Analysis for Monitoring Dynamic Protein Interaction and Folding in a Living Cell2021

    • Author(s)
      MIYAZAKI Ryoji、MORI Hiroyuki、AKIYAMA Yoshinori
    • Journal Title

      Seibutsu Butsuri

      Volume: 61 Issue: 1 Pages: 036-039

    • DOI

      10.2142/biophys.61.036

    • NAID

      130007976685

    • ISSN
      0582-4052, 1347-4219
    • Language
      Japanese
    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-20K15715
  • [Journal Article] Reversible auto-inhibitory regulation of Escherichia coli metallopeptidase BepA for selective β-barrel protein degradation.2020

    • Author(s)
      Daimon 他7名
    • Journal Title

      Proc. Natl. Acad. Sci. USA.

      Volume: 117 Issue: 45 Pages: 27989-27996

    • DOI

      10.1073/pnas.2010301117

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-18K05396, KAKENHI-PROJECT-19K06562, KAKENHI-PROJECT-20K06556, KAKENHI-PROJECT-20K15715, KAKENHI-PROJECT-18H02386, KAKENHI-PROJECT-18H02404, KAKENHI-PROJECT-17K07334, KAKENHI-PROJECT-18K06136, KAKENHI-PROJECT-19K22395, KAKENHI-PROJECT-18KK0197, KAKENHI-PROJECT-18H02405
  • [Journal Article] Fine interaction profiling of VemP and mechanisms responsible for its translocation-coupled arrest-cancelation2020

    • Author(s)
      Miyazaki Ryoji、Akiyama Yoshinori、Mori Hiroyuki
    • Journal Title

      eLife

      Volume: 9

    • DOI

      10.7554/elife.62623

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-20K06556, KAKENHI-PROJECT-20K15715, KAKENHI-PROJECT-18H02404, KAKENHI-PROJECT-17K07334
  • [Journal Article] A photo-cross-linking approach to monitor protein dynamics in living cells2019

    • Author(s)
      Miyazaki Ryoji、Akiyama Yoshinori、Mori Hiroyuki
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - General Subjects

      Volume: 印刷中 Issue: 2 Pages: 129317-129317

    • DOI

      10.1016/j.bbagen.2019.03.003

    • Peer Reviewed
    • Data Source
      KAKENHI-PUBLICLY-17H05666, KAKENHI-PUBLICLY-17H05879, KAKENHI-PROJECT-19K21179, KAKENHI-PROJECT-20K06556, KAKENHI-PROJECT-17K07334
  • [Presentation] PpiD/YfgM複合体はSec複合体とDsbAを連結する2023

    • Author(s)
      宮崎 亮次
    • Organizer
      2022年度国立遺伝学研究所研究会「単細胞生物に見られる生体プロセスの恒常性維持システム」
    • Invited
    • Data Source
      KAKENHI-PROJECT-20K15715
  • [Presentation] PpiD/YfgM複合体はSec複合体とDsbAを連結する2023

    • Author(s)
      宮崎 亮次
    • Organizer
      2022年度国立遺伝学研究所研究会「単細胞生物に見られる生体プロセスの恒常性維持システム」
    • Invited
    • Data Source
      KAKENHI-PROJECT-22K15061
  • [Presentation] 内膜タンパク質PpiDはSecトランスロコンとDsbAを連結し、膜透過基質タンパク質へのジスルフィド結合導入を助ける2023

    • Author(s)
      宮崎亮次、鈴木健裕、堂前直、塚崎智也
    • Organizer
      第96回日本生化学会大会
    • Data Source
      KAKENHI-PROJECT-22K15061
  • [Presentation] タンパク質膜透過・輸送に関わるPpiD/YfgM複合体の in vivo 光架橋解析2022

    • Author(s)
      宮崎 亮次、鈴木 健裕、堂前 直、塚崎 智也
    • Organizer
      第22回 日本蛋白質科学会年会
    • Invited
    • Data Source
      KAKENHI-PROJECT-22K15061
  • [Presentation] in vivo光架橋法によるタンパク質膜透過関連因子PpiDの相互作用解析2022

    • Author(s)
      宮崎 亮次、鈴木 健裕、堂前 直、塚崎 智也
    • Organizer
      第18回 21世紀大腸菌研究会
    • Data Source
      KAKENHI-PROJECT-20K15715
  • [Presentation] in vivo光架橋法による内膜タンパク質複合体PpiD/YfgMの機能解析2022

    • Author(s)
      宮崎亮次
    • Organizer
      2021年度国立遺伝学研究所研究会「単細胞システムの複製と維持における生体高分子の機能」
    • Invited
    • Data Source
      KAKENHI-PROJECT-20K15715
  • [Presentation] PpiD/YfgM複合体はSec複合体とDsbAを連結する2022

    • Author(s)
      宮崎 亮次
    • Organizer
      2022年度国立遺伝学研究所研究会「単細胞生物に見られる生体プロセスの恒常性維持システム」
    • Invited
    • Data Source
      KAKENHI-PROJECT-21KK0126
  • [Presentation] タンパク質膜透過・輸送に関わるPpiD/YfgM複合体の in vivo 光架橋解析2022

    • Author(s)
      宮崎 亮次、鈴木 健裕、堂前 直、塚崎 智也
    • Organizer
      第22回 日本蛋白質科学会年会
    • Invited
    • Data Source
      KAKENHI-PROJECT-20K15715
  • [Presentation] in vivo光架橋法による内膜タンパク質複合体PpiD/YfgMの機能解析2022

    • Author(s)
      宮崎 亮次
    • Organizer
      2021年度国立遺伝学研究所研究会 「単細胞システムの複製と維持における生体高分子の機能」
    • Data Source
      KAKENHI-PROJECT-21KK0126
  • [Presentation] Structural and functional analysis of a thiosulfate transporter complex2022

    • Author(s)
      Ikei Mai、Takeuchi Azusa、Naito Yusuke、Ichikawa Muneyoshi、Miyazaki Ryoji、Tsukazaki Tomoya
    • Organizer
      The 22nd Annual Scientific Meeting of the Nitric Oxide Society of Japan
    • Int'l Joint Research
    • Data Source
      KAKENHI-PUBLICLY-22H05567
  • [Presentation] タンパク質膜透過・輸送に関わるPpiD/YfgM複合体の in vivo 光架橋解析2022

    • Author(s)
      宮崎 亮次、鈴木 健裕、堂前 直、塚崎 智也
    • Organizer
      第22回 日本蛋白質科学会年会
    • Data Source
      KAKENHI-PROJECT-21KK0126
  • [Presentation] in vivo光架橋法によるタンパク質膜透過関連因子PpiDの相互作用解析2022

    • Author(s)
      宮崎 亮次、鈴木 健裕、堂前 直、塚崎 智也
    • Organizer
      第18回 21世紀大腸菌研究会
    • Data Source
      KAKENHI-PROJECT-22K15061
  • [Presentation] in vivo光架橋法によるタンパク質膜透過関連因子PpiDの相互作用解析2022

    • Author(s)
      宮崎 亮次、鈴木 健裕、堂前 直、塚崎 智也
    • Organizer
      第18回 21世紀大腸菌研究会
    • Data Source
      KAKENHI-PROJECT-21KK0126
  • [Presentation] 改良型部位特異的in vivo光架橋法による生細胞内での翻訳途上ポリペプチド鎖の相互作用動態解析2019

    • Author(s)
      宮崎亮次、秋山芳展、森博幸
    • Organizer
      第92回 日本生化学会大会
    • Data Source
      KAKENHI-PROJECT-19K21179
  • [Presentation] Analysis of cis and trans factors involved in export and translation arrest of VemP by an in vivo photo cross-linking approach2018

    • Author(s)
      Ryoji Miyazaki, Yoshinori Akiyama, Hiroyuki Mori
    • Organizer
      International symposium Proteins: From the Cradle to the Grave
    • Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-19K21179
  • 1.  Shiota Takuya (20819304)
    # of Collaborated Projects: 2 results
    # of Collaborated Products: 0 results
  • 2.  田岡 東 (20401888)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 3.  竹田 弘法 (80816588)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 4.  荒磯 裕平 (20753726)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 5.  青木 英莉子 (80822874)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 6.  森 博幸
    # of Collaborated Projects: 0 results
    # of Collaborated Products: 2 results

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