YOSHIHARA Eisaku 良原栄策

… Alternative Names

石井純子イシイジュンコ

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Researcher Number	70167063
Other IDs
Affiliation (based on the past Project Information) *help	2011: 東海大学, 医学部, 准教授 2007 – 2008: Tokai University, 医学部, 准教授 1993 – 2006: 東海大学, 医学部, 助教授 2005: Tokai University, Molecular Life Science, Associate Professor, 総合医学研究所, 助教授 1993: 東海大学, 医学部, 研究員 … More 1990 – 1992: 東海大学, 医学部, 講師 1988: 東海大学, 医学部・細胞情報科学, 助手 1986 – 1988: 東海大学, 医学部, 助手 Less
Review Section/Research Field	Principal Investigator Bacteriology (including Mycology) / 細菌学 / Bacteriology (including Mycology) Except Principal Investigator Bacteriology (including Mycology) / 細菌学 / Biological Sciences / Structural biochemistry / Applied genomics
Keywords	Principal Investigator 緑膿菌 / 外膜 / Pseudomonas aeruginosa / protein D2 / 多剤耐性 / MexAB-OprM / ポーリン / 薬剤排出ポンプ / protease / porin … More / プロテアーゼ / モノクローナル抗体 / 阻害剤 / 多剤排出ポンプ / チャンネル蛋白 / inhibitors / 院内感染 / FRET / multidrug efflux pump / multidrug resistance / OprM / OprE3 / OprD / 触媒基 / 部位特異的変異 / チャネル / 細胞外ループ / ポ-リン / monoclonal antibodies / extracellular loops / oligomeric structure / outer membrane channel / 薬剤感受性 / 阻害物質 / 外服チャネル / オリゴマー構造 / 外膜チャネル / protein-protein interaction / subunit / MexAB-OprM pump / drug efflux pump / HlyBD-TolC / アフィニティカラム / ヒスチジンタグ / タンパク間相互作用 / サブユニット / MexAB-OprMポンプ / maltoporin / membrane topology / α-helix / channel / tolC / トポロジー決定法 / マルトポーリン / 膜トポロジー / α-ヘリックス / チャンネル / protein crystal / nucleotide sequence / catalytic residues / 部位特異的変異導入 / 塩基配列 / タンパク質結晶 / 全塩基配列 / mutifunctional protein / catalytic residue / site-directed mutagenesis / outer membrane protein / 塩基性アミノ酸 / ptoteinD2 / 多機能タンパク質 / proteinD2 / 外膜タンパク質 / imipenem / gating / channel protein / outer membrane / 調節因子 / Ca^<2+>イオン / OprD2 / イミペネム / ゲート開閉 / 薬剤耐性 / ポーリン蛋白 / 物質透過性 / ポンプ阻害剤 / ペプチド抗原 / 多剤耐性化 / 感染症 / ペプチド / 抗体 / 開閉調節 / カルシウム / ゲート / 孔開閉 / ドメイン構造 / チャンネル蛋白質 / ドメイン / 分子集合 … More Except Principal Investigator 薬剤耐性 / 緑膿菌 / 抗生物質 / 膜蛋白 / 外膜 / 排出 / Membrane protein / 細菌 / 耐性 / 異物 / ポンプ / 人工膜 / 透過性 / 透過孔 / bacteria / antibiotic / transporter / RND / MexB / 膜貫通領域 / 排出ポンプ / トランスポーター / 薬物 / 院内感染 / Efflux / Pump / 異物排出 / 細胞膜 / 輸送 / トポロジー / Antibiotic resistance / Pseudomonas / 膜蛋白質 / ポーリン / Reconstitution / Drug resistance / Outer membrane / ポ-リン / 日和見感染 / 排除限界 / pore / barrier / permeability / opportunistic infection / gram-negative pathogen / outer membrane / 孔 / 細菌膜 / cell membrane / membrane protein / Psudomonas / xenobiotic antibiotic / efflux pump / drug resistance / 変異体 / エネルギー / X線解析 / 結晶構造 / 抗生物質・異物 / membrane proteins / drug / resistance / pump / efflux / 多剤耐性 / RND-ファミリー / MexAB-OprM / 基質認識 / Infection / X-ray diffraction / Crystal / Antibiotics / X線回析 / X-線結晶回析 / X-線結晶解析 / 結晶 / Transport / Membrane / Membrane topology / 生体防御 / Channel / Porin / Lipid bilayr / Pseudomonas aeruginosa / 脂質平面膜 / イミペネム / OprD2 / チャンネル / Cloning / Planar membrane / Liposome / Permeability / リポソ-ム / 再構成 / クロ-ニング / 平面膜 / エンドサイトーシス / 神経伝達物質 / シナプス小胞 / ゲノムワイド相関解析(GWAS) / 酵母ツーハイブリッド法 / シンタキシン / シナプトガミン / NETO / シナプトタグミン / コンタクチン / 疾患モデルマウス / 感受性遺伝子 / GWAS / ネットワーク解析 / 相関解析 / マイクロサテライト / 摂食障害 Less

Genome-wide identification of genes for anorexia and their cascade analysis on disease development by functional study
- Principal Investigator
  
  INOKO Hidetoshi
- Project Period (FY)
  2007 – 2011
- Research Category
  
  Grant-in-Aid for Scientific Research (S)
- Research Field
  
  Applied genomics
- Research Institution
  Tokai University
Development of antibodies and small molecules as inhibitors ofPseudomonas aeruginosa multidrug efflux pumpsPrincipal Investigator
- Principal Investigator
  
  YOSHIHARA Eisaku
- Project Period (FY)
  2007 – 2008
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Bacteriology (including Mycology)
- Research Institution
  Tokai University
Discovery of the extracellular loops being essential for the proper function of the multidrug efflux pump suggests that the loops may be an excellent target for the pump inhibitorPrincipal Investigator
- Principal Investigator
  
  YOSHIHARA Eisaku
- Project Period (FY)
  2005 – 2006
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Bacteriology (including Mycology)
- Research Institution
  Tokai University
The elucidation of the molecular assembly mechanism of the multidrug efflux pump and the development of the screening system for pump inhibitorsPrincipal Investigator
- Principal Investigator
  
  YOSHIHARA Eisaku
- Project Period (FY)
  2002 – 2003
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Bacteriology (including Mycology)
- Research Institution
  Tokai University
Structure and function of the xenobiotic-antibiotic extrusion transporters
- Principal Investigator
  
  NAKAE Taiji
- Project Period (FY)
  2001 – 2005
- Research Category
  
  Grant-in-Aid for Scientific Research on Priority Areas
- Review Section
  
  Biological Sciences
- Research Institution
  Tokai University
Structure and function of the xenobiotic-multidrug efflux pump
- Principal Investigator
  
  NAKAE Taiji
- Project Period (FY)
  2000 – 2002
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  Bacteriology (including Mycology)
- Research Institution
  Tokai University
The outer membrane components of xenobiotic efflux pumps are discovered to be members of a novel channel family with the unique structurePrincipal Investigator
- Principal Investigator
  
  YOSHIHARA Eisaku
- Project Period (FY)
  1999 – 2000
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Bacteriology (including Mycology)
- Research Institution
  Tokai University
Structure and Function of the Antibiotic-Specific Channel
- Principal Investigator
  
  NAKAE Taiji
- Project Period (FY)
  1998 – 1999
- Research Category
  
  Grant-in-Aid for Scientific Research (B).
- Research Field
  
  Structural biochemistry
- Research Institution
  Tokai University
The xenobiotic efflux pumps and multiantibiotic resistance in Pseudomonas aeruginosa
- Principal Investigator
  
  NAKAE Taiji
- Project Period (FY)
  1997 – 1999
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  Bacteriology (including Mycology)
- Research Institution
  Tokai University
Elucidation of the molecular structure of OprD porin bearing the protease activity and the discovery of theporin homologous with OprDPrincipal Investigator
- Principal Investigator
  
  YOSHIHARA Eisaku
- Project Period (FY)
  1997 – 1998
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Bacteriology (including Mycology)
- Research Institution
  Tokai University
Porin bearing the protease activity : its structure and functionPrincipal Investigator
- Principal Investigator
  
  YOSHIHARA Eisaku
- Project Period (FY)
  1995 – 1996
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Bacteriology (including Mycology)
- Research Institution
  Tokai University
The permeability specificity of the imipenem-specific channel of OprD2 in Pseudomonas aeruginosa
- Principal Investigator
  
  NAKAE Taiji
- Project Period (FY)
  1993 – 1994
- Research Category
  
  Grant-in-Aid for General Scientific Research (B)
- Research Field
  
  Bacteriology (including Mycology)
- Research Institution
  Tokai University School of Medicine
Molecular and structural mechanism of the gating of the porin channelPrincipal Investigator
- Principal Investigator
  
  YOSHIHARA Eisaku
- Project Period (FY)
  1993 – 1994
- Research Category
  
  Grant-in-Aid for General Scientific Research (C)
- Research Field
  
  Bacteriology (including Mycology)
- Research Institution
  Tokai University
ポーリンチャンネル開閉の分子機構Principal Investigator
- Principal Investigator
  
  良原栄策
- Project Period (FY)
  1992
- Research Category
  
  Grant-in-Aid for General Scientific Research (C)
- Research Field
  
  細菌学
- Research Institution
  Tokai University
緑膿菌ポ-リン蛋白質のドメイン構造とその機能Principal Investigator
- Principal Investigator
  
  良原栄策
- Project Period (FY)
  1991
- Research Category
  
  Grant-in-Aid for General Scientific Research (C)
- Research Institution
  Tokai University
緑膿菌ポ-リン蛋白質の分子集合と機能発現Principal Investigator
- Principal Investigator
  
  良原栄策
- Project Period (FY)
  1990
- Research Category
  
  Grant-in-Aid for General Scientific Research (C)
- Research Field
  
  細菌学
- Research Institution
  Tokai University
Role of the outer membrane permeability in the antibiotic resistance in Pseudomonas aerugionsa
- Principal Investigator
  
  NAKAE Taiji
- Project Period (FY)
  1990 – 1991
- Research Category
  
  Grant-in-Aid for General Scientific Research (B)
- Research Field
  
  細菌学
- Research Institution
  Tokai University
新しく同定した緑膿菌外膜のポーリン群の構造、物質透過機序及びその生物学的意義Principal Investigator
- Principal Investigator
  
  良原栄策
- Project Period (FY)
  1988
- Research Category
  
  Grant-in-Aid for General Scientific Research (C)
- Research Field
  
  細菌学
- Research Institution
  Tokai University
Role of the outer membrane of gram-negativebacteria in the intrinsic drug resistance
- Principal Investigator
  
  NAKAE Taiji
- Project Period (FY)
  1987 – 1988
- Research Category
  
  Grant-in-Aid for General Scientific Research (C)
- Research Field
  
  細菌学
- Research Institution
  Tokai University School of Medicine
緑膿菌外膜の透過孔と抗生物質透過性の関連
- Principal Investigator
  
  NAKAE Taiji
- Project Period (FY)
  1986
- Research Category
  
  Grant-in-Aid for General Scientific Research (C)
- Research Field
  
  細菌学
- Research Institution
  Tokai University

All 2009 2008 2007 2006 2005 2004 2003 2002

All Journal Article Presentation Patent

[Journal Article] Modification of a human monoclonal antibody Fab fragment specific for Plasmodium falciparum 19-kDa C-terminal merozoite surface protein 1 by site-directed mutagenesis2008
- Author(s)
  Tao,Y.-L., Chen, X.-J., Fu, Y.-F., Tsukamoto, H., Yoshihara, E.Tachibana, H.
- Journal Title
  
  Parasitol. Res. 103
  
  Pages: 429-433
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19590458
[Journal Article] Diversity in the oligomeric channel structure of the multidrug efflux pumps in Pseudomonas aeruginosa2007
- Author(s)
  Yoshihara, E.
- Journal Title
  
  Microbiol. Immunol. 51・1
  
  Pages: 47-52
- NAID
  10020281830
- Description
  「研究成果報告書概要(和文)」より
- Data Source
  KAKENHI-PROJECT-17590402
[Journal Article] Diversity in the oligomeric channel structure of the multidrug efflux pumps in Pseudomonas aeruginosa2007
- Author(s)
  Yoshihara, E., Eda, S.
- Journal Title
  
  Microbiol.Immunol. 51(1)
  
  Pages: 47-52
- NAID
  10020281830
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-17590402
[Journal Article] Diversity in the oligomeric channel structure of the multidrug efflux pumps in Pseudomonas aeruginosa2007
- Author(s)
  Yoshihara, E., Eda, S.
- Journal Title
  
  Microbiol. Immunol. 51
  
  Pages: 47-52
- NAID
  10020281830
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19590458
[Journal Article] Diversity in the oligomeric channel structure of the multidrug efflux pumps in Pseudomonas aeruginosa2007
- Author(s)
  Yoshihara, E.
- Journal Title
  
  Microbiol.Immunol. 51.1
  
  Pages: 47-52
- NAID
  10020281830
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19590458
[Journal Article] Assignment of the outer-membrane-subunit-selective domain of the membrane fusion protein in the tripartite xenobiotic efflux pump of Pseudomonas aeruginosa2006
- Author(s)
  Eda, S., Maseda, H., Yoshihara, E., Nakae T.
- Journal Title
  
  FEMS Microbiol. Letts. 254
  
  Pages: 101-107
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-13142209
[Journal Article] Assignment of the outer-membrane-subunit- selective domain of the membrane fusion protein in the tripartite xenobiotic efflux pump of Pseudomonas aeruginosa2006
- Author(s)
  Eda, S., Maseda, H., Yoshihara, E., Nalae, T.
- Journal Title
  
  FEMS Microbiol.Lett. 254
  
  Pages: 101-107
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-17590402
[Journal Article] Multidrug transporter MexB of Pseudomonas aeruginosa : overexpression, purification, and initial structural characterization 20062005
- Author(s)
  Mokhonov, v., Mokhonova, E., Yoshihara,....Akama, H., Nakae, T.
- Journal Title
  
  Protein Expression and Purification 40
  
  Pages: 91-100
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-13142209
[Journal Article] Multidrug transporter MexB of Pseudomonas aeruginosa : overexpression, purification, and initial structural characterization2005
- Author(s)
  Mokohonov, V., Mokohonova, E, Yoshihara, E., Masui, R., Sakai, M., Akama, H., Nakae, T.
- Journal Title
  
  Protein Expr.Purif. 40
  
  Pages: 91-100
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-17590402
[Journal Article] MexZ-mediated regulation of mexXY multidrug efflux pumpexpression in Pseudomonas aeruginosa by binding on the mexZ-MexX intergenic DNA2004
- Author(s)
  Matsuo, Y., Eda, S., Gotoh, N., Yoshihara, E., Nakae, T.
- Journal Title
  
  FEMS Microbiol. Lett. 238
  
  Pages: 23-28
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-13142209
[Journal Article] Linkage of the efflux-pump expression level with substrate extrusion rate in the MexAB-OprM efflux pump of Pseudomonas aeruginosa2003
- Author(s)
  Narita, S., Eda, S., Yoshihara, E., Nakae, T.
- Journal Title
  
  Biochem. Biophys. Res. Commun. 308
  
  Pages: 922-926
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-13142209
[Journal Article] Mutations affecting DNA-binding activity of the MexR repressor of mexR-mexA-mexB-oprM operon expression 20042003
- Author(s)
  Saito, K., Akama, H., Yoshihara, E., Nakae, T.
- Journal Title
  
  J. Bacterio1. 183
  
  Pages: 6195-6198
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-13142209
[Journal Article] The outer membrane component of the multiddrug efflux poump from Pseudomonas aeruginosa may be a gated channel. 20032002
- Author(s)
  Yoshihara, E., Maseda, H., Saito, K.
- Journal Title
  
  Eur. J. Biochem. 269
  
  Pages: 4738-4745
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-13142209
[Journal Article] The outer membrane component of the multiddrug efflux poump from Pseudomonas aeruginosa may be a gated channel.2002
- Author(s)
  Yoshihara, E.
- Journal Title
  
  Eur.J.Biochem. 269
  
  Pages: 4738-4745
- Description
  「研究成果報告書概要(和文)」より
- Data Source
  KAKENHI-PROJECT-13142209
[Journal Article] A novel assembly process of the multicomponent xenobiotic efflux pump in Pseudomonas aeruginosa2002
- Author(s)
  Maseda, H., Kitao, M., Eda, S., Yoshihara, E, Nakae, T.
- Journal Title
  
  Molec. Microbiol. 46
  
  Pages: 677-686
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-13142209
[Patent]2009
- Inventor(s)
  猪子英俊、良原栄策、光永滋樹
- Industrial Property Rights Holder
  東海大学
- Filing Date
  2009-06-16
- Data Source
  KAKENHI-PROJECT-19101008
[Patent] 緑膿菌の薬剤排出ポンプの機能を阻害する方法及び薬剤2006
- Inventor(s)
  良原栄策, 猪子英俊
- Industrial Property Rights Holder
  学校法人東海大学
- Industrial Property Number
  2006-033522
- Filing Date
  2006-02-10
- Data Source
  KAKENHI-PROJECT-17590402
[Patent] 緑膿菌の薬剤排出ポンプの機能を阻害する方法及び薬剤2006
- Inventor(s)
  良原栄策, 猪子英俊
- Industrial Property Rights Holder
  学校法人東海大学
- Industrial Property Number
  2006-033522
- Filing Date
  2006-02-10
- Description
  「研究成果報告書概要(和文)」より
- Data Source
  KAKENHI-PROJECT-17590402
[Presentation] Novel peptidyl inhibitor of MexAB-OprM, a multidrug efflux pump in Pseudomonas aeruginosa2009
- Author(s)
  良原栄策
- Organizer
  第82回日本細菌学会総会
- Place of Presentation
  名古屋国際会議場
- Year and Date
  2009-03-12
- Data Source
  KAKENHI-PROJECT-19590458
[Presentation] Novel peptidyl inhibitor of MexAB-OprM, a multidrug efflux pump in Pseudomonas aeruginosa2009
- Author(s)
  良原栄策
- Organizer
  日本細菌学会総会
- Place of Presentation
  名古屋国際会議場
- Data Source
  KAKENHI-PROJECT-19590458
[Presentation] 緑膿菌多剤排出ポンプMexAB-OprMの外膜チャネルであるOprMが関与する基質認識2008
- Author(s)
  良原栄策
- Organizer
  第80回日本細菌学会総会
- Place of Presentation
  大阪アジア太平洋トレードセンター
- Year and Date
  2008-03-27
- Data Source
  KAKENHI-PROJECT-19590458
[Presentation] 緑膿菌多剤排出ポンプMexAB-OprMの外膜チャネルであるOprMが関与する基質認識2007
- Author(s)
  良原栄策
- Organizer
  日本細菌学会総会
- Place of Presentation
  アジア太平洋トレードセンタ
- Data Source
  KAKENHI-PROJECT-19590458

1. NAKAE Taiji (50102851)

# of Collaborated Projects: 8 results

# of Collaborated Products: 6 results
2. YONEYAMA Hiroshi (10220774)

# of Collaborated Projects: 6 results

# of Collaborated Products: 0 results
3. ISHII Junko (20212813)

# of Collaborated Projects: 2 results

# of Collaborated Products: 0 results
4. NARITA Shin-ichiro (30338751)

# of Collaborated Projects: 2 results

# of Collaborated Products: 1 results
5. KOBAYASHI Yoriko

# of Collaborated Projects: 2 results

# of Collaborated Products: 0 results
6. INOKO Hidetoshi (10101932)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
7. OKA Akira (80384866)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
8. ENDOH Takaho (40384862)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
9. OHTSUKA Masato (90372945)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
10. HIRAYAMA Noriaki (70238393)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
11. TANI Ayako (70284867)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
12. SATAKE Sachiko

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
13. GABRIEL Rumm

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
14. TILMAN Schir

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
15. JURG P Rosen

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results

YOSHIHARA Eisaku 良原 栄策

石井 純子 イシイ ジュンコ

Research Projects

Research Products

Co-Researchers

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Development of antibodies and small molecules as inhibitors ofPseudomonas aeruginosa multidrug efflux pumpsPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Discovery of the extracellular loops being essential for the proper function of the multidrug efflux pump suggests that the loops may be an excellent target for the pump inhibitorPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

The elucidation of the molecular assembly mechanism of the multidrug efflux pump and the development of the screening system for pump inhibitorsPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

The outer membrane components of xenobiotic efflux pumps are discovered to be members of a novel channel family with the unique structurePrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Elucidation of the molecular structure of OprD porin bearing the protease activity and the discovery of theporin homologous with OprDPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Porin bearing the protease activity : its structure and functionPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Molecular and structural mechanism of the gating of the porin channelPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

ポーリンチャンネル開閉の分子機構Principal Investigator

Principal Investigator

Project Period (FY)

YOSHIHARA Eisaku 良原栄策

石井純子イシイジュンコ