HARAGUCHI Seiki 原口清輝

Researcher Number	10324576
Other IDs
Affiliation (Current)	2025: 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 上級研究員
Affiliation (based on the past Project Information) *help	2021 – 2024: 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 上級研究員 2013 – 2014: 独立行政法人農業・食品産業技術総合研究機構, 畜産草地研究所家畜育種繁殖研究領域, 主任研究員 2011: 千葉県がんセンター(研究所), 研究員 2001 – 2004: 滋賀医科大学, 医学部, 助手
Review Section/Research Field	Principal Investigator Laboratory animal science / Developmental biology Except Principal Investigator Basic Section 42010:Animal production science-related / Biological Sciences / Experimental pathology / Pediatrics
Keywords	Principal Investigator ES細胞 / マウス / ブタ / ニワトリ / テラトーマ / 異種間キメラ / トランスジェニックマウス / siRNA / dsRNA / RNAi … More Except Principal Investigator … More アポトーシス / ブタ / ゲノム編集 / ガラス化保存 / Drs / ノックアウトマウス / 癌抑制遺伝子 / 未成熟卵子 / DNA修復 / 卵子 / DNA修復機能 / 胚盤胞発生 / CD46 / OCT4 / DNA二本鎖切断 / ブタ接合体 / ブタ卵子 / Knockout mouse / Apoptosis / Tumor suppressor gene / UNC5D / 分化 / 依存性受容体 / 神経芽腫 / drs遺伝子 / 足場非依存性増殖 / がん抑制遺伝子 Less

Efficient production of genome edited pigs from cryopreserved immature oocytes
- Principal Investigator
  
  ソムファイタマス
- Project Period (FY)
  2024 – 2027
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 42010:Animal production science-related
- Research Institution
  National Agriculture and Food Research Organization
Study of DNA damage and repair mechanism in porcine embryos obtained from vitrified oocytes and zygotes, and its relevance to genome editing
- Principal Investigator
  
  Somfai Tamas
- Project Period (FY)
  2021 – 2023
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 42010:Animal production science-related
- Research Institution
  National Agriculture and Food Research Organization
Evaluation of stem cell multipotency by interspecific chimera productionPrincipal Investigator
- Principal Investigator
  
  HARAGUCHI Seiki
- Project Period (FY)
  2013 – 2014
- Research Category
  
  Grant-in-Aid for Challenging Exploratory Research
- Research Field
  
  Laboratory animal science
- Research Institution
  National Agriculture and Food Research Organization
Functional role of the dependence receptor UNC5D in regulation Of cell death in neuroblastoma
- Principal Investigator
  
  LI Yuanyuan
- Project Period (FY)
  2009 – 2011
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Pediatrics
- Research Institution
  Chiba Cancer Center (Research Institute)
Functional analyses of novel tumor suppressor genes by gene targeting
- Principal Investigator
  
  INOUE Hirokazu
- Project Period (FY)
  2003 – 2004
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Experimental pathology
- Research Institution
  Shiga University of Medical Science
Drs遺伝子機能と足場非依存性増殖の分子機構
- Principal Investigator
  
  井上寛一
- Project Period (FY)
  2002
- Research Category
  
  Grant-in-Aid for Scientific Research on Priority Areas
- Review Section
  
  Biological Sciences
- Research Institution
  Shiga University of Medical Science
dsRNAトランスジェニックマウスにおけるRNAi効果の検討Principal Investigator
- Principal Investigator
  
  原口清輝
- Project Period (FY)
  2002 – 2003
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Developmental biology
- Research Institution
  Shiga University of Medical Science
Drs遺伝子機能と足場非依存性増殖の分子機構
- Principal Investigator
  
  INOUE Hirokazu
- Project Period (FY)
  2001
- Research Category
  
  Grant-in-Aid for Scientific Research on Priority Areas (C)
- Review Section
  
  Biological Sciences
- Research Institution
  Shiga University of Medical Science

All 2023 2022 2021 2009 2004 2003 Other

All Journal Article Presentation

[Journal Article] Electroporation‐mediated genome editing in vitrified/warmed porcine zygotes obtained in vitro2023
- Author(s)
  Haraguchi Seiki、Dang‐Nguyen Thanh Q.、Kikuchi Kazuhiro、Somfai Tam?s
- Journal Title
  
  Molecular Reproduction and Development
  
  Volume: 91 Issue: 1
- DOI
  10.1002/mrd.23712
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-21K05912
[Journal Article] Vitrification of porcine immature oocytes and zygotes results in different levels of DNA damage which reflects developmental competence to the blastocyst stage2023
- Author(s)
  Somfai Tam?s、Haraguchi Seiki、Dang-Nguyen Thanh Quang、Kaneko Hiroyuki、Kikuchi Kazuhiro
- Journal Title
  
  PLOS ONE
  
  Volume: 18 Issue: 3 Pages: e0282959-e0282959
- DOI
  10.1371/journal.pone.0282959
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-21K05912
[Journal Article] Specific gene silencing in the pre-implantation stage mouse embryo by an siRNA expression vector system.2004
- Author(s)
  Haraguchi, S et al.
- Journal Title
  
  Mol.Reprod.Dev. 68
  
  Pages: 17-24
- NAID
  10012348758
- Data Source
  KAKENHI-PROJECT-15590335
[Journal Article] Specific gene silencing in the pre-implantation stage mouse embryo by a siRNA expression vector system2004
- Author(s)
  Haraguchi, S.et al.
- Journal Title
  
  Mol.Reprod.Dev 68
  
  Pages: 17-24
- NAID
  10012348758
- Description
  「研究成果報告書概要(和文)」より
- Data Source
  KAKENHI-PROJECT-15590335
[Journal Article] Specific gene silencing in the pre-implantation stage mouse embryo by an siRNA expression vector system.2004
- Author(s)
  Haraguchi, S., Saga, Y, Naito, K., Inoue, H., Seto, A.
- Journal Title
  
  Mol.Reprod.Dev. 68
  
  Pages: 17-24
- NAID
  10012348758
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-15590335
[Journal Article] A novel apoptotic pathway induced by the drs tumor suppressor gene.2004
- Author(s)
  Tambe, Y., Isono, T..Haraguchi, S., Yoshioka-Yamashita, A, Yutsudo, M.,, Inoue.H.
- Journal Title
  
  Oncogene 23
  
  Pages: 2977-2987
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-15590335
[Journal Article] Conserved role of nanos proteins in germ cell development.2003
- Author(s)
  Tsuda, M., Sasaoka, Y., Kiso, M., Abe, K., Haraguchi, S., Kobayashi, S., Saga, Y.
- Journal Title
  
  Science 301
  
  Pages: 1239-1241
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-15590335
[Journal Article] nanos1 : a mouse nanos gene expressed in the central nervous system is dispensable for normal development.2003
- Author(s)
  Haraguchi, S., Tsuda, M., Kitajima, S., Sasaoka, Y, Nomura-Kitabayashid, A., Kurokawa, K., Saga, Y.
- Journal Title
  
  Mech.Dev. 120
  
  Pages: 721-731
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-15590335
[Journal Article] Nanos 1 : a mouse nanos gene expressed in the central nerves system is dispensable for normal development2003
- Author(s)
  Haraguchi, S.et al.
- Journal Title
  
  Mech.Dev. 120
  
  Pages: 721-731
- Description
  「研究成果報告書概要(和文)」より
- Data Source
  KAKENHI-PROJECT-15590335
[Presentation] Vitrification of porcine immature oocytes and zygotes results in different levels of DNA damage in subsequently developing embryos.2022
- Author(s)
  Somfai T, Haraguchi S, Dang-Nguyen T.Q., Kikuchi K.
- Organizer
  International Congress on Animal Reproduction
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-21K05912
[Presentation] Application of Assisted Reproductive Techniques for the Preservation of Indigenous Pigs.2022
- Author(s)
  Somfai T, Haraguchi H, Dang-Nguyen T, Kikuchi K.
- Organizer
  The 6th Fatty Pig International Conference
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-21K05912
[Presentation] Vitrification of porcine immature oocytes causes DNA double-strand breaks in subsequently developing embryos2021
- Author(s)
  ソムファイタマス, 原口清輝, Dang-Nguyen TQ, 金子浩之, 菊地和弘
- Organizer
  Cryopreservation Conference 2021 (Online)
- Data Source
  KAKENHI-PROJECT-21K05912
[Presentation] Functional Role of Dependence Receptor UNC5D in Regulating Programmed Cell Death and Survival in Neuroblastoma2009
- Author(s)
  Yuanyuan Li, Yuyan Zhu, Miki Ohira, Seiki Haraguchi, Yohko Nakamura, Kiyoshi Asada, Toshikazu Ushijima, Atsuko Nakagawa, Hirofumi Arakawa, Akira Nakagawara
- Organizer
  第68回日本癌学会総会
- Place of Presentation
  パシフィコ横浜(横浜)
- Year and Date
  2009-10-01
- Data Source
  KAKENHI-PROJECT-21591378
[Presentation] ニワトリ胚を用いたマウスES細胞の多能性評価
- Author(s)
  松原悠子、細江実佐、原口清輝
- Organizer
  日本畜産学会第119会大会
- Place of Presentation
  宇都宮大学峰キャンパス（宇都宮市）
- Year and Date
  2015-03-28 – 2015-03-30
- Data Source
  KAKENHI-PROJECT-25640056
[Presentation] 異種間キメラによる幹細胞の多能性評価
- Author(s)
  原口清輝、松原悠子、細江実佐
- Organizer
  第62回日本実験動物学会総会
- Place of Presentation
  京都テルサ（京都府民総合交流プラザ）（京都市）
- Year and Date
  2015-05-28 – 2015-05-30
- Data Source
  KAKENHI-PROJECT-25640056

1. INOUE Hirokazu (30176440)

# of Collaborated Projects: 3 results

# of Collaborated Products: 2 results
2. Somfai Tamas (90547720)

# of Collaborated Projects: 2 results

# of Collaborated Products: 5 results
3. LI Yuanyuan (00392259)

# of Collaborated Projects: 1 results

# of Collaborated Products: 1 results
4. NAKAMURA Ohko (60260254)

# of Collaborated Projects: 1 results

# of Collaborated Products: 1 results
5. NAKAGAWARA Akira (50117181)

# of Collaborated Projects: 1 results

# of Collaborated Products: 1 results
6. MATSUBARA Yuko (90391573)

# of Collaborated Projects: 1 results

# of Collaborated Products: 2 results
7. 菊地和弘 (20360456)

# of Collaborated Projects: 1 results

# of Collaborated Products: 5 results
8. 千本正一郎 (60372661)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
9. 山下敦子

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
10. 吉岡敦子(山下)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results

HARAGUCHI Seiki 原口 清輝

Research Projects

Research Products

Co-Researchers

Efficient production of genome edited pigs from cryopreserved immature oocytes

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Study of DNA damage and repair mechanism in porcine embryos obtained from vitrified oocytes and zygotes, and its relevance to genome editing

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Evaluation of stem cell multipotency by interspecific chimera productionPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Functional role of the dependence receptor UNC5D in regulation Of cell death in neuroblastoma

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Functional analyses of novel tumor suppressor genes by gene targeting

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Drs遺伝子機能と足場非依存性増殖の分子機構

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

dsRNAトランスジェニックマウスにおけるRNAi効果の検討Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Drs遺伝子機能と足場非依存性増殖の分子機構

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

[Journal Article] Electroporation‐mediated genome editing in vitrified/warmed porcine zygotes obtained in vitro2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Vitrification of porcine immature oocytes and zygotes results in different levels of DNA damage which reflects developmental competence to the blastocyst stage2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Specific gene silencing in the pre-implantation stage mouse embryo by an siRNA expression vector system.2004

Author(s)

Journal Title

NAID

Data Source

[Journal Article] Specific gene silencing in the pre-implantation stage mouse embryo by a siRNA expression vector system2004

Author(s)

Journal Title

NAID

Description

Data Source

[Journal Article] Specific gene silencing in the pre-implantation stage mouse embryo by an siRNA expression vector system.2004

Author(s)

Journal Title

NAID

Description

Data Source

[Journal Article] A novel apoptotic pathway induced by the drs tumor suppressor gene.2004

HARAGUCHI Seiki 原口清輝