Danev Radostin ＤａｎｅｖＲａｄｏｓｔｉｎ

Researcher Number	50415931
Other IDs
Affiliation (Current)	2026: 東京大学, 大学院医学系研究科(医学部), 教授
Affiliation (based on the past Project Information) *help	2026: 東京大学, 大学院医学系研究科(医学部), 教授 2021 – 2024: 東京大学, 大学院医学系研究科(医学部), 教授 2018 – 2019: 東京大学, 大学院医学系研究科(医学部), 教授
Review Section/Research Field	Principal Investigator Basic Section 43020:Structural biochemistry-related / Basic Section 43020:Structural biochemistry-related / 0701:Biology at molecular to cellular levels, and related fields Except Principal Investigator Biological Sciences
Keywords	Principal Investigator GPCR / cryo-EM / data acquisition / single particle analysis / cryo-electron microscopy / deep learning / sample screening / machine learning / automation / structure … More / cryo-tomography / cryo-electron tomography / visual proteomics / in situ / cryo-FIB / cryo-ET / molecular structure … More Except Principal Investigator TJ-MAPs / TJ-ストランド / アピカル細胞骨格 / TJ-アピカル複合体 / 細胞間バリア / TJストランド / クライオ電顕 / アピカル骨格 / TJMAP / 液-液相分離（LLPS） / アピカル膜 / クライオ電顕トモグラフィー / バリア病 / 細胞骨格 / 上皮細胞 / クローディン / タイトジャンクション / 上皮バリア Less

Enhancing cryo-EM performance through real-time beam modulationPrincipal Investigator
- Principal Investigator
  
  Danev Radostin
- Project Period (FY)
  2026 – 2028
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 43020:Structural biochemistry-related
- Research Institution
  The University of Tokyo
Intelligent cryo-electron microscopy of G protein-coupled receptorsPrincipal Investigator
- Principal Investigator
  
  Danev Radostin
- Project Period (FY)
  2024
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 43020:Structural biochemistry-related
- Research Institution
  The University of Tokyo
New cryo-electron microscopy method for elucidation of G-protein coupled receptor supercomplexesPrincipal Investigator
- Principal Investigator
  
  Danev Radostin
- Project Period (FY)
  2021 – 2022
- Research Category
  
  Grant-in-Aid for JSPS Fellows
- Review Section
  
  Basic Section 43020:Structural biochemistry-related
- Research Institution
  The University of Tokyo
Novel approach focusing on the tight junction-based paracellular barrier combined with the apical barrier toward understanding and manipulating epithelial barriers
- Principal Investigator
  
  Tsukita Sachiko
- Project Period (FY)
  2019 – 2023
- Research Category
  
  Grant-in-Aid for Specially Promoted Research
- Review Section
  
  Biological Sciences
- Research Institution
  Teikyo University
  Osaka University
Adaptive optics approach for high-throughput, high-resolution cryo-electron microscopy of biological macromolecules and complexesPrincipal Investigator
- Principal Investigator
  
  Danev Radostin
- Project Period (FY)
  2018 – 2019
- Research Category
  
  Grant-in-Aid for Research Activity Start-up
- Review Section
  
  0701:Biology at molecular to cellular levels, and related fields
- Research Institution
  The University of Tokyo

All 2025 2024 2023 2022 2021 2020 2019 2018

All Journal Article Presentation Book

[Book] Histones Methods and Protocols, Chapter 6, High-Resolution Cryo-EM Analyses of Nucleosomes2025
- Author(s)
  Yoshimasa Takizawa, Cheng-Han Ho, Shoko Sato, Radostin Danev, Hitoshi Kurumizaka
- Total Pages
  292
- Publisher
  Humana Press
- ISBN
  9781071644850
- Data Source
  KAKENHI-PROJECT-23K23818
[Journal Article] The Hitchdock Domain in Kinesin-2 Tail Enables Adaptor Assembly and Cargo Binding2025
- Author(s)
  Jiang Xuguang、Danev Radostin、Niu Baichun、Ohtsuki Sumio、Yanagisawa Haruaki、Hirokawa Nobutaka、Kikkawa Masahide
- Journal Title
  
  bioRxiv
  
  Volume: -
- DOI
  10.1101/2025.03.21.644525
- Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K23818, KAKENHI-PROJECT-24KF0141
[Journal Article] Cryo-EM Structure of the Human Amylin 1 Receptor in Complex with CGRP and Gs Protein2024
- Author(s)
  Cao Jianjun、Belousoff Matthew J.、Danev Radostin、Christopoulos Arthur、Wootten Denise、Sexton Patrick M.
- Journal Title
  
  Biochemistry
  
  Volume: 63 Issue: 9 Pages: 1089-1096
- DOI
  10.1021/acs.biochem.4c00114
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K23818
[Journal Article] Smart parallel automated cryo-electron tomography2024
- Author(s)
  Eisenstein Fabian、Fukuda Yoshiyuki、Danev Radostin
- Journal Title
  
  Nature Methods
  
  Volume: 21 Issue: 9 Pages: 1612-1615
- DOI
  10.1038/s41592-024-02373-9
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K23818
[Journal Article] Pharmacological hallmarks of allostery at the M4 muscarinic receptor elucidated through structure and dynamics2023
- Author(s)
  Vuckovic Z、Wang J、Pham V、Mobbs J、Belousoff M、Bhattarai A、Burger W、Thompson G、Yeasmin M、Nawaratne V、Leach K、van der Westhuizen E、Khajehali E、Liang Y、Glukhova A、Wootten D、Lindsley C、Tobin A、Sexton P、Danev R、Valant C、Miao Y、Christopoulos A、Thal David M
- Journal Title
  
  eLife
  
  Volume: 12 Pages: 83477-83477
- DOI
  10.7554/elife.83477
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K23818
[Journal Article] Structural insight into selectivity of amylin and calcitonin receptor agonists2023
- Author(s)
  Cao Jianjun、Belousoff Matthew J.、Gerrard Elliot、Danev Radostin、Fletcher Madeleine M.、Dal Maso Emma、Schreuder Herman、Lorenz Katrin、Evers Andreas、Tiwari Garima、Besenius Melissa、Li Ziyu、Johnson Rachel M.、Wootten Denise、Sexton Patrick M.
- Journal Title
  
  Nature Chemical Biology
  
  Volume: 20 Issue: 2 Pages: 162-169
- DOI
  10.1038/s41589-023-01393-4
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K23818
[Journal Article] Xanomeline displays concomitant orthosteric and allosteric binding modes at the M4 mAChR2023
- Author(s)
  Burger Wessel A. C.、Pham Vi、Vuckovic Ziva、Powers Alexander S.、Mobbs Jesse I.、Laloudakis Yianni、Glukhova Alisa、Wootten Denise、Tobin Andrew B.、Sexton Patrick M.、Paul Steven M.、Felder Christian C.、Danev Radostin、Dror Ron O.、Christopoulos Arthur、Valant Celine、Thal David M.
- Journal Title
  
  Nature Communications
  
  Volume: 14 Issue: 1 Pages: 5440-5440
- DOI
  10.1038/s41467-023-41199-5
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K23818
[Journal Article] Parallel cryo electron tomography on in situ lamellae2022
- Author(s)
  Eisenstein Fabian、Yanagisawa Haruaki、Kashihara Hiroka、Kikkawa Masahide、Tsukita Sachiko、Danev Radostin
- Journal Title
  
  Nature Methods
  
  Volume: 20 Issue: 1 Pages: 131-138
- DOI
  10.1038/s41592-022-01690-1
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-21F20764, KAKENHI-PROJECT-23K23818, KAKENHI-PROJECT-19H05468
[Journal Article] Routine sub-2.5A cryo-EM structure determination of GPCRs2021
- Author(s)
  Danev Radostin、Belousoff Matthew、Liang Yi-Lynn、Zhang Xin、Eisenstein Fabian、Wootten Denise、Sexton Patrick M.
- Journal Title
  
  Nature Communications
  
  Volume: 12 Issue: 1 Pages: 4333-4333
- DOI
  10.1038/s41467-021-24650-3
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-21F20764
[Journal Article] Structure and Dynamics of Adrenomedullin Receptors AM1 and AM2 Reveal Key Mechanisms in the Control of Receptor Phenotype by Receptor Activity-Modifying Proteins2020
- Author(s)
  Liang Yi-Lynn、Belousoff Matthew J.、Fletcher Madeleine M.、Zhang Xin、Khoshouei Maryam、Deganutti Giuseppe、Koole Cassandra、Furness Sebastian G. B.、Miller Laurence J.、Hay Debbie L.、Christopoulos Arthur、Reynolds Christopher A.、Danev Radostin、Wootten Denise、Sexton Patrick M.
- Journal Title
  
  ACS Pharmacology & Translational Science
  
  Volume: 3 Issue: 2 Pages: 263-284
- DOI
  10.1021/acsptsci.9b00080
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K21176
[Journal Article] Activation of the GLP-1 receptor by a non-peptidic agonist2020
- Author(s)
  Zhao Peishen、Liang Yi-Lynn、Belousoff Matthew J.、Deganutti Giuseppe、Fletcher Madeleine M.、Willard Francis S.、Bell Michael G.、Christe Michael E.、Sloop Kyle W.、Inoue Asuka、Truong Tin T.、Clydesdale Lachlan、Furness Sebastian G. B.、Christopoulos Arthur、Wang Ming-Wei、et al
- Journal Title
  
  Nature
  
  Volume: 577 Issue: 7790 Pages: 432-436
- DOI
  10.1038/s41586-019-1902-z
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K08264, KAKENHI-PROJECT-19K21176
[Journal Article] Toward a Structural Understanding of Class B GPCR Peptide Binding and Activation2020
- Author(s)
  Liang Yi-Lynn、Belousoff Matthew J.、Zhao Peishen、Koole Cassandra、Fletcher Madeleine M.、Truong Tin T.、Julita Villy、Christopoulos George、Xu H. Eric、Zhang Yan、Khoshouei Maryam、Christopoulos Arthur、Danev Radostin、Sexton Patrick M.、Wootten Denise
- Journal Title
  
  Molecular Cell
  
  Volume: 77 Issue: 3 Pages: 656-668.e5
- DOI
  10.1016/j.molcel.2020.01.012
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K21176
[Journal Article] Cryo-EM Methodology: Current Aspects and Future Directions2019
- Author(s)
  Radostin Danev, Haruaki Yanagisawa, Masahide Kikkawa
- Journal Title
  
  Trends in Biochemical Sciences
  
  Volume: 印刷中
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K21176
[Journal Article] Cryo-Electron Microscopy Methodology: Current Aspects and Future Directions2019
- Author(s)
  Danev Radostin、Yanagisawa Haruaki、Kikkawa Masahide
- Journal Title
  
  Trends in Biochemical Sciences
  
  Volume: 44 Issue: 10 Pages: 837-848
- DOI
  10.1016/j.tibs.2019.04.008
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K21176
[Journal Article] Improved applicability and robustness of fast cryo-electron tomography data acquisition2019
- Author(s)
  Eisenstein Fabian、Danev Radostin、Pilhofer Martin
- Journal Title
  
  Journal of Structural Biology
  
  Volume: 208 Issue: 2 Pages: 107-114
- DOI
  10.1016/j.jsb.2019.08.006
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K21176
[Presentation] Parallel cryo-electron tomography and beyond2025
- Author(s)
  Radostin Danev
- Organizer
  Tsinghua Spring School on Cryo-ET with Dynamo
- Invited
- Data Source
  KAKENHI-PROJECT-23K23818
[Presentation] Optimizing the performance and throughput of cryo-EM single particle analysis and tomography2024
- Author(s)
  Radostin Danev
- Organizer
  2024 International Symposium on Cryo‐Electron Microscopy Technology
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K23818
[Presentation] Optimizing the performance and throughput of cryo-EM single particle analysis and tomography2024
- Author(s)
  Radostin Danev
- Organizer
  DBS Colloquium, National University of Singapore
- Invited
- Data Source
  KAKENHI-PROJECT-23K23818
[Presentation] Performance and practical aspects of CryoARM 200HR electron cryo-microscope2024
- Author(s)
  Radostin Danev
- Organizer
  The 80th Annual Meeting of the Japanese Society of Microscopy
- Data Source
  KAKENHI-PROJECT-23K23818
[Presentation] Addressing Experimental Challenges in Single Particle Analysis and Cryo-Tomography2024
- Author(s)
  Radostin Danev
- Organizer
  2024 Challenges in Structural Biology Summit
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K23818
[Presentation] Performance and practical aspects of CryoARM 200HR electron cryo-microscope2024
- Author(s)
  Radostin Danev
- Organizer
  2024 NIPS-TARA Cryo-EM Workshop
- Data Source
  KAKENHI-PROJECT-23K23818
[Presentation] Optimizing the performance and throughput of cryo-EM2023
- Author(s)
  Radostin Danev
- Organizer
  The 20th International Microscopy Congress
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K23818
[Presentation] Optimizing the performance and throughput of cryo-EM2023
- Author(s)
  Radostin Danev
- Organizer
  2023 NIPS Cryo-EM Workshop
- Invited
- Data Source
  KAKENHI-PROJECT-23K23818
[Presentation] Advancing the performance and throughput of cryo-EM2023
- Author(s)
  Radostin Danev
- Organizer
  The 96th Annual Meeting of the Japanese Biochemical Society
- Invited
- Data Source
  KAKENHI-PROJECT-23K23818
[Presentation] Optimizing cryo-EM for GPCRs and first steps in cryo-tomography2022
- Author(s)
  Radostin Danev
- Organizer
  Frontier of Dynamic Structural Biology, IPR, Osaka
- Invited
- Data Source
  KAKENHI-PROJECT-23K23818
[Presentation] Pushing the performance limits of cryo-EM for membrane proteins2022
- Author(s)
  Radostin Danev
- Organizer
  THE 4TH INTERNATIONAL SYMPOSIUM OF STRUCTURE-BASED DRUG DISCOVERY
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K23818
[Presentation] Optimizing cryo-EM for GPCRs and Parallel cryo electron tomography on in situ lamellae2022
- Author(s)
  Radostin Danev
- Organizer
  ExCells seminar, Okazaki
- Invited
- Data Source
  KAKENHI-PROJECT-23K23818
[Presentation] Optimizing cryo-EM for GPCRs and first steps in cryo-tomography2022
- Author(s)
  Radostin Danev
- Organizer
  The 43rd Annual Meeting of the Japan Society for the Study of Obesity International Satellite Symposium
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K23818
[Presentation] Optimizing cryo-EM for GPCRs and first steps in cryo-tomography2022
- Author(s)
  Radostin Danev
- Organizer
  ARC CCeMMP Symposium, “Cryo-electron microscopy of membrane proteins”
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K23818
[Presentation] What makes a great cryo-EM?2019
- Author(s)
  Radostin Danev
- Organizer
  Future of Cryo-EM Developments Meeting
- Invited
- Data Source
  KAKENHI-PROJECT-19K21176
[Presentation] Phase Plates in Cryo-EM2019
- Author(s)
  Radostin Danev
- Organizer
  EMBL Cryo-EM in Industry and Academia Workshop
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K21176
[Presentation] Cryo-EM of Membrane Receptors2019
- Author(s)
  Radostin Danev
- Organizer
  92nd annual meeting of the Japanese biochemical society
- Invited
- Data Source
  KAKENHI-PROJECT-19K21176
[Presentation] Electron Optics and Image Formation2019
- Author(s)
  Radostin Danev
- Organizer
  Cryo-Electron Microscopy Course at OIST
- Invited
- Data Source
  KAKENHI-PROJECT-19K21176
[Presentation] Phase plates in cryo-EM2019
- Author(s)
  Radostin Danev
- Organizer
  EMBL Cryo-EM in Industry and Academia workshop
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K21176
[Presentation] Recent Cryo-EM Advancements2019
- Author(s)
  Radostin Danev
- Organizer
  1st Cryo-EM Network User Group Meeting
- Invited
- Data Source
  KAKENHI-PROJECT-19K21176
[Presentation] Cryo-EM of Membrane Proteins With and Without Phase Plates2019
- Author(s)
  Radostin Danev
- Organizer
  71st joint JSCB and PSSJ meeting
- Invited
- Data Source
  KAKENHI-PROJECT-19K21176
[Presentation] Practical Performance of the Volta Phase Plate2019
- Author(s)
  Radostin Danev
- Organizer
  Gordon Research Conference on Three Dimensional Electron Microscopy
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K21176
[Presentation] Performance Contributions of Energy Filtering and Phase Plates for Single Particle Analysis of Membrane Proteins2019
- Author(s)
  Radostin Danev
- Organizer
  NIPS EM Workshop 2019
- Invited
- Data Source
  KAKENHI-PROJECT-19K21176
[Presentation] Cryo-EM with and without phase plates2018
- Author(s)
  Radostin Danev
- Organizer
  Japanese Society of Microscopy Soft Materials Branch Meeting
- Invited
- Data Source
  KAKENHI-PROJECT-19K21176

1. Tsukita Sachiko (00188517)

# of Collaborated Projects: 1 results

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2. 中村駿 (80882779)

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3. 後藤慎平 (50747219)

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4. 近藤玄 (40243258)

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5. 田村淳 (00362525)

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6. 田中啓雄 (70795905)

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7. 柏原宏香 (20966692)

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8. 八木健 (10241241)

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9. 足澤悦子 (00446262)

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10. 足立誠 (30335244)

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11. 鈴木貴 (40114516)

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12. 矢野智樹 (20546121)

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13. 入江克雅 (20415087)

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14. EISENSTEIN FABIAN

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15. INOUE Asuka

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Danev Radostin Ｄａｎｅｖ Ｒａｄｏｓｔｉｎ

Research Projects

Research Products

Co-Researchers

Enhancing cryo-EM performance through real-time beam modulationPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Intelligent cryo-electron microscopy of G protein-coupled receptorsPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

New cryo-electron microscopy method for elucidation of G-protein coupled receptor supercomplexesPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Novel approach focusing on the tight junction-based paracellular barrier combined with the apical barrier toward understanding and manipulating epithelial barriers

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Adaptive optics approach for high-throughput, high-resolution cryo-electron microscopy of biological macromolecules and complexesPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

[Book] Histones Methods and Protocols, Chapter 6, High-Resolution Cryo-EM Analyses of Nucleosomes2025

Author(s)

Total Pages

Publisher

ISBN

Data Source

[Journal Article] The Hitchdock Domain in Kinesin-2 Tail Enables Adaptor Assembly and Cargo Binding2025

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Cryo-EM Structure of the Human Amylin 1 Receptor in Complex with CGRP and Gs Protein2024

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Smart parallel automated cryo-electron tomography2024

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Pharmacological hallmarks of allostery at the M4 muscarinic receptor elucidated through structure and dynamics2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Structural insight into selectivity of amylin and calcitonin receptor agonists2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Xanomeline displays concomitant orthosteric and allosteric binding modes at the M4 mAChR2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Parallel cryo electron tomography on in situ lamellae2022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Routine sub-2.5A cryo-EM structure determination of GPCRs2021

Author(s)

Journal Title

DOI

Data Source

Danev Radostin ＤａｎｅｖＲａｄｏｓｔｉｎ