Nishihara Ryo 西原諒

Researcher Number	50846988
Other IDs	https://orcid.org/0000-0002-9539-596X
Affiliation (Current)	2026: 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員
Affiliation (based on the past Project Information) *help	2023 – 2025: 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 2020 – 2022: 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究員
Review Section/Research Field	Principal Investigator Basic Section 37030:Chemical biology-related / Medium-sized Section 37:Biomolecular chemistry and related fields Except Principal Investigator Basic Section 34020:Analytical chemistry-related
Keywords	Principal Investigator ルシフェラーゼ / ルシフェリン / 生物発光 / 酵素 / 酵素反応 / 抗体 / ペプチド / ELISA / 血清アルブミン / セレンテラジン … More / 発光 / 化学センサー / タンパク質 / 液滴 / 基質 … More Except Principal Investigator 人工ルシフェリン / ウイルス / シアロ糖鎖 / ナノマイクロシステム / 生物発光 / マイクロデバイス / 分析試薬 / バイオ分析 / 新型コロナウイルス Less

Luminescent enzymatic reactions catalyzed by molecular assemblies and their bioimaging applicationsPrincipal Investigator
- Principal Investigator
  
  西原諒
- Project Period (FY)
  2025 – 2028
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 37030:Chemical biology-related
- Research Institution
  National Institute of Advanced Industrial Science and Technology
人工ルシフェリン含有小胞体への感染をミミックしたウイルス検知
- Principal Investigator
  
  栗田僚二
- Project Period (FY)
  2025 – 2027
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 34020:Analytical chemistry-related
- Research Institution
  National Institute of Advanced Industrial Science and Technology
Establishment of an enzyme-free bioluminescent immunoassayPrincipal Investigator
- Principal Investigator
  
  西原諒
- Project Period (FY)
  2025 – 2027
- Research Category
  
  Grant-in-Aid for Challenging Research (Exploratory)
- Review Section
  
  Medium-sized Section 37:Biomolecular chemistry and related fields
- Research Institution
  National Institute of Advanced Industrial Science and Technology
Development of artificial luciferin and its application to digital bioanalysis
- Principal Investigator
  
  Kurita Ryoji
- Project Period (FY)
  2024
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 34020:Analytical chemistry-related
- Research Institution
  National Institute of Advanced Industrial Science and Technology
Physicochemical control of enzymatic luminescence reactionPrincipal Investigator
- Principal Investigator
  
  Nishihara Ryo
- Project Period (FY)
  2022 – 2024
- Research Category
  
  Grant-in-Aid for Early-Career Scientists
- Review Section
  
  Basic Section 37030:Chemical biology-related
- Research Institution
  National Institute of Advanced Industrial Science and Technology
Development of luminescence probes for specific detection of human proteinsPrincipal Investigator
- Principal Investigator
  
  Nishihara Ryo
- Project Period (FY)
  2020 – 2021
- Research Category
  
  Grant-in-Aid for Early-Career Scientists
- Review Section
  
  Basic Section 37030:Chemical biology-related
- Research Institution
  National Institute of Advanced Industrial Science and Technology

All 2025 2024 2023 2021 2020

All Journal Article Presentation Book Patent

[Book] 「Design of Coelenterazine Analogue to Reveal Bioluminescent Reaction of Human Serum Albumin」Book chapter in Bioluminescence2021
- Author(s)
  Nishihara Ryo、Niwa Kazuki、Tomita Tatsunosuke、Kurita Ryoji
- Total Pages
  13
- Publisher
  IntechOpen
- Data Source
  KAKENHI-PROJECT-20K15421
[Journal Article] Alteration in the Sensitivity of Firefly Bioluminescence to pH Driven by Molecular Recruitment to Coacervate Droplets2025
- Author(s)
  Yoshiki Kihara, Shunsuke Tomita, Kazuki Niwa, Ryoji Kurita, and Ryo Nishihara
- Journal Title
  
  ACS Omega
  
  Volume: - Issue: 24 Pages: 25313-25321
- DOI
  10.1021/acsomega.4c11259
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-22K14802
[Journal Article] Dry chemistry utilizing artificial luciferin for human serum albumin quantification2025
- Author(s)
  Kurita Ryoji、Nishihara Ryo
- Journal Title
  
  Sensors and Actuators B: Chemical
  
  Volume: 423 Pages: 136700-136700
- DOI
  10.1016/j.snb.2024.136700
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23K23382
[Journal Article] Discovery of Pseudo-Luciferase Activity in Immunoglobulin G (IgG) and Its Application to the Detection of IgG Denaturation2025
- Author(s)
  Ryo Nishihara, Yoshiki Kihara, Eiji Yamamoto, Yoshinori Hirano, Ryoji Kurita
- Journal Title
  
  Analytical Chemistry
  
  Volume: 97 Issue: 18 Pages: 9935-9943
- DOI
  10.1021/acs.analchem.5c00646
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22K14802
[Journal Article] Pseudo-Luciferase Activity of the SARS-CoV-2 Spike Protein for Cypridina Luciferin2024
- Author(s)
  Nishihara Ryo、Dokainish Hisham M.、Kihara Yoshiki、Ashiba Hiroki、Sugita Yuji、Kurita Ryoji
- Journal Title
  
  ACS Central Science
  
  Volume: 10 Issue: 2 Pages: 283-290
- DOI
  10.1021/acscentsci.3c00887
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23K23382
[Journal Article] Coelenterazine Analogue with Human Serum Albumin-Specific Bioluminescence2020
- Author(s)
  Nishihara Ryo、Niwa Kazuki、Tomita Tatsunosuke、Kurita Ryoji
- Journal Title
  
  Bioconjugate Chemistry
  
  Volume: 31 Issue: 12 Pages: 2679-2684
- DOI
  10.1021/acs.bioconjchem.0c00536
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-20K05719, KAKENHI-PROJECT-20K15421
[Patent] 発光基質化合物2020
- Inventor(s)
  西原諒、栗田僚二
- Industrial Property Rights Holder
  国立研究開発法人産業技術総合研究所
- Industrial Property Rights Type
  特許
- Industrial Property Number
  2020-046137
- Filing Date
  2020
- Data Source
  KAKENHI-PROJECT-20K15421
[Presentation] SARS-CoV-2スパイクタンパク質の擬似ルシフェラーゼ活性2025
- Author(s)
  西原諒、Hisham M. Dokainish、木原良樹、杉田有治、栗田僚二
- Organizer
  つくば医工連携フォーラム2025
- Data Source
  KAKENHI-PROJECT-23K23382
[Presentation] 非発光タンパク質における擬似ルシフェラーゼ活性の発見と利用2024
- Author(s)
  西原諒、栗田僚二
- Organizer
  日本化学会第104春季年会
- Data Source
  KAKENHI-PROJECT-23K23382
[Presentation] SARS-CoV-2スパイクタンパク質の擬似ルシフェラーゼ活性2024
- Author(s)
  西原諒、Hisham M. Dokainish、木原良樹、杉田有治、栗田僚二
- Organizer
  LS-BT合同研究発表会
- Data Source
  KAKENHI-PROJECT-23K23382
[Presentation] Pseudoluciferase activity of the SARS-CoV-2 spike protein for Cypridina luciferin2024
- Author(s)
  西原諒、Hisham M. Dokainish、木原良樹、杉田有治、栗田僚二
- Organizer
  22nd ISBC & 20th ISLS
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K23382
[Presentation] 擬似ルシフェラーゼ活性の発見とタンパク質分析への応用2024
- Author(s)
  西原諒
- Organizer
  MA-T学会第2回年会
- Invited
- Data Source
  KAKENHI-PROJECT-22K14802
[Presentation] ウミホタルルシフェリンに対するSARS-CoV-2スパイクタンパク質の擬似ルシフェラーゼ活性2024
- Author(s)
  西原諒、Hisham M. Dokainish、木原良樹、杉田有治、栗田僚二
- Organizer
  第 18 回バイオ関連化学シンポジウム 2024
- Data Source
  KAKENHI-PROJECT-23K23382
[Presentation] Pseudo-luciferase activity of the SARS-CoV-2 spike protein2024
- Author(s)
  西原諒、Hisham M. Dokainish、木原良樹、杉田有治、栗田僚二
- Organizer
  21st IUPAB Congress 2024 (IUPAB2024)
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K23382
[Presentation] タンパク質に隠された発光機能2024
- Author(s)
  西原諒
- Organizer
  第36回高分子学会関東支部茨城地区若手の会交流会
- Invited
- Data Source
  KAKENHI-PROJECT-22K14802
[Presentation] ヒトタンパク質の疑似ルシフェラーゼ活性を利用した迅速分析2024
- Author(s)
  西原諒、栗田僚二
- Organizer
  第84回分析化学討論会
- Invited
- Data Source
  KAKENHI-PROJECT-23K23382
[Presentation] Discovery and utilization of pseudo-luciferase activities in non-bioluminescent proteins2023
- Author(s)
  西原諒、栗田僚二
- Organizer
  RSC Tokyo International Conference 2023
- Data Source
  KAKENHI-PROJECT-23K23382

1. Kurita Ryoji (50415676)

# of Collaborated Projects: 2 results

# of Collaborated Products: 10 results
2. 南木創 (40793980)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
3. 小島直 (30356985)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
4. 上野祐子 (30589627)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
5. 冨田辰之介

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results

Nishihara Ryo 西原 諒

Research Projects

Research Products

Co-Researchers

Luminescent enzymatic reactions catalyzed by molecular assemblies and their bioimaging applicationsPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

人工ルシフェリン含有小胞体への感染をミミックしたウイルス検知

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Establishment of an enzyme-free bioluminescent immunoassayPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Development of artificial luciferin and its application to digital bioanalysis

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Physicochemical control of enzymatic luminescence reactionPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Development of luminescence probes for specific detection of human proteinsPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

[Book] 「Design of Coelenterazine Analogue to Reveal Bioluminescent Reaction of Human Serum Albumin」Book chapter in Bioluminescence2021

Author(s)

Total Pages

Publisher

Data Source

[Journal Article] Alteration in the Sensitivity of Firefly Bioluminescence to pH Driven by Molecular Recruitment to Coacervate Droplets2025

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Dry chemistry utilizing artificial luciferin for human serum albumin quantification2025

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Discovery of Pseudo-Luciferase Activity in Immunoglobulin G (IgG) and Its Application to the Detection of IgG Denaturation2025

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Pseudo-Luciferase Activity of the SARS-CoV-2 Spike Protein for Cypridina Luciferin2024

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Coelenterazine Analogue with Human Serum Albumin-Specific Bioluminescence2020

Author(s)

Journal Title

DOI

Data Source

[Patent] 発光基質化合物2020

Inventor(s)

Industrial Property Rights Holder

Industrial Property Rights Type

Industrial Property Number

Filing Date

Data Source

[Presentation] SARS-CoV-2スパイクタンパク質の擬似ルシフェラーゼ活性2025

Author(s)

Organizer

Nishihara Ryo 西原諒