Nishii Wataru 西井亘

… Alternative Names

西井亘ニシイワタル

NISHII Wataru 西井亘

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Researcher Number	30287461
Other IDs
External Links
Affiliation (based on the past Project Information) *help	2018: 国立研究開発法人理化学研究所, 生命機能科学研究センター, 客員主管研究員 2015 – 2017: 国立研究開発法人理化学研究所, 横山構造生物学研究室, 研究員 2007: The Institute of Physical and Chemical Research, タンパク質基盤研究グループ, 研究員 2006: 独立行政法人理化学研究所, タンパク質構造研究チーム, 研究員 2002 – 2005: 東京薬科大学, 生命科学部, 助手 1997 – 2000: 東京薬科大学, 生命科学部, 助手
Review Section/Research Field	Principal Investigator Functional biochemistry / Functional biochemistry / Basic Section 43030:Functional biochemistry-related Except Principal Investigator Structural biochemistry / Cell biology
Keywords	Principal Investigator プロテオリシス / 細胞内タンパク質分解 / レドックス / X線結晶構造 / 基質特異性 / ATP依存性プロテアーゼ / 活性制御 / ４次構造 / アロステリックジスルフィド結合 / レドックス制御 … More / 結晶構造 / 腸内細菌 / X線小角散乱解析 / X線結晶解析 / 腸内細菌科 / AAA+プロテアーゼ / 細胞内プロテオリシス / タンパク質分解 / リボソームタンパク質 / 部位特異的変異 / 大量発現 / リボソーム / プロセッシブ分解 / 大腸菌 / ポリリン酸 / アミノ酸飢餓 / 細胞内イメージング / ラット海馬 / 細胞死 / 可視化解析 / 蛍光基質 … More Except Principal Investigator CAAXモチーフ特異的プロテアーゼ / Proteinase / Membrane-bound proteinase / Cathepsin E / Colopsin / Hepsin / CAAX-motif-specific proteinase / Progastrin-cleaving proteinase / Brain-gut proteinase / 金属プロテアーゼ / 基質特異性 / α-キモトリプシン / トリプシン / CAAX-モチーフ特異的プロテアーゼ / セリンプロテアーゼ / 膜結合性プロテアーゼ / カテプシンE遺伝子 / エンテロペプチダーゼ / プロテアーゼ / 膜結合プロテアーゼ / カテプシンE / コロプシン / ヘプシン / プロガストリン分解プロテアーゼ / 脳・腸管系プロテアーゼ / FRET(蛍光共鳴エネルギー転移) / 細胞内プロテアーゼ / 二重蛍光標識基質 / カスパーゼ / コラゲナーゼ / 細胞内注入 / アクリロダン標識基質 / 蛍光基質 / グルタミン酸刺激 / 細胞死 / カスパーゼ1 / 海馬スライス培養 / FRET / 二重蛍光基質 / 可視化解析 / 細胞内プロテオリシス Less

Regulatory mechanisms of the AAA+ Lon proteasePrincipal Investigator
- Principal Investigator
  
  西井亘
- Project Period (FY)
  2018 – 2020
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 43030:Functional biochemistry-related
- Research Institution
  Institute of Physical and Chemical Research
Redox regulation of proteolysis in the Enterobacteriacea cellsPrincipal Investigator
- Principal Investigator
  
  Nishii Wataru
- Project Period (FY)
  2015 – 2017
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Functional biochemistry
- Research Institution
  Institute of Physical and Chemical Research
大腸菌ATP依存性プロテアーゼLonおよびHslVUの基質切断機構Principal Investigator
- Principal Investigator
  
  西井亘
- Project Period (FY)
  2005 – 2007
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Functional biochemistry
- Research Institution
  The Institute of Physical and Chemical Research
  Tokyo University of Pharmacy and Life Science
ATP依存性プロテアーゼによる生理基質機能部位の優先切断機構Principal Investigator
- Principal Investigator
  
  西井亘
- Project Period (FY)
  2002 – 2004
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Functional biochemistry
- Research Institution
  Tokyo University of Pharmacy and Life Science
蛍光基質を用いた細胞内プロテオリシスの可視化解析Principal Investigator
- Principal Investigator
  
  西井亘
- Project Period (FY)
  1999 – 2000
- Research Category
  
  Grant-in-Aid for Encouragement of Young Scientists (A)
- Research Field
  
  Functional biochemistry
- Research Institution
  Tokyo University of Pharmacy and Life Science
生細胞における蛋白質分解過程の可視化解析
- Principal Investigator
  
  TAKAHASHI Kenji
- Project Period (FY)
  1998 – 2000
- Research Category
  
  Grant-in-Aid for Exploratory Research
- Research Field
  
  Cell biology
- Research Institution
  Tokyo University of Pharmacy and Life Science
Structural, Functional and Physiological Characterization of Novel Brain-Gut Proteinases
- Principal Investigator
  
  TAKAHASHI Kenji
- Project Period (FY)
  1997 – 1998
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  Structural biochemistry
- Research Institution
  Tokyo University of Pharmacy and Life Science

All 2017 2016 2015 2008 2007 2005 2004

All Journal Article Presentation Patent

[Journal Article] SARS-CoV 3CL protease cleaves its C-terminal autoprocessing site by novel subsite cooperativity2016
- Author(s)
  Muramatsu, T., Takemoto, C., Kim, Y.-T., Wang, H., Nishii, W., Terada, T. , Shirouzu, M., Yokoyama, S.
- Journal Title
  
  Proc. Natl Acad. Sci. USA
  
  Volume: 113 Pages: 12997-13002
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-15K07019
[Journal Article] Substrate Specificities of Porcine and Bovine Enterpeptidases toward the Peptide Val-(Asp)4-Lys-Ile-Val-Gly and Its Analogs2008
- Author(s)
  Kim, Y.-T., Nishii, W., Matsushima, S., Inoue, H. Ito, H. Park S.-J. and Takahashi, K.
- Journal Title
  
  Bioscience, Biotechnology, and Biochemistry 印刷中
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17770116
[Journal Article] The effects of mutations in the carboxyl-teminal region on the catalytic activity of Escherichi a coli signal peptidase I.2008
- Author(s)
  Kim YT, Yoshida H, Kojima M, Kurita R, Nishii W, Muramatsu T, Ito H, Park SJ, Takahashi K.
- Journal Title
  
  Journal of Biochemistry 143
  
  Pages: 237-242
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17770116
[Journal Article] Inhibition and stabilization of Aspergilloglutamic peptidase by the propeptide : Identication of critical sequences and residues in the propeptide.2005
- Author(s)
  Kubota, K., Nishii, W., Kojima, M., Takahashi, K.
- Journal Title
  
  Journal of Biological Chemistry 280
  
  Pages: 999-1006
- Data Source
  KAKENHI-PROJECT-14780495
[Journal Article] Cleavage mechanism of ATP-dependent Lon protease toward ribosomal S2 protein2005
- Author(s)
  Nishii, W., Suzuki, T., Nakada, M., Kim, Y.-T., Muramatsu, T., Takahashi, K.
- Journal Title
  
  FEBS Letters 579
  
  Pages: 6846-6850
- Data Source
  KAKENHI-PROJECT-17770116
[Journal Article] Identification of arginine residues important for the activity of Eschericha coli signal peptidase I2004
- Author(s)
  Kim, Y.-T., Kurita, R., Kojima, M., Nishii, W., Tnokura, M., Muramatsu, T., Ito, H., Takahashi K.
- Journal Title
  
  Biological Chemistry 386
  
  Pages: 381-388
- Data Source
  KAKENHI-PROJECT-14780495
[Patent] ウィルスプロテアーゼ生産方法2007
- Inventor(s)
  横山茂之, 村松知成, 金龍泰, 西井亘
- Industrial Property Rights Holder
  独立行政法人理化学研究所
- Industrial Property Number
  2007-003242
- Filing Date
  2007-11-22
- Data Source
  KAKENHI-PROJECT-17770116
[Presentation] ダイナミックな構造変化を介したAAA+プロテアーゼLonの制御メカニズム2017
- Author(s)
  西井亘、藤井佳史、松村義隆、仙石徹、小島正樹、横山茂之
- Organizer
  生物学会年会・第90回日本生化学会大会　合同大会（ConBio2017）
- Data Source
  KAKENHI-PROJECT-15K07019
[Presentation] Redox regulation of Lon protease activity by a disulfide bond in facultative anaerobes2016
- Author(s)
  Nishii, W., Seki, E., Yanagisawa, T., Sehngoku, T., Terada, T., Murmatsu, T., Niino-Kukimoto, M, Shirouzu, M., Kojima, M., Kihara, H. & Yokoyama, S.
- Organizer
  FASEB Science Research Conference
- Place of Presentation
  コロラド（USA）
- Year and Date
  2016-08-17
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K07019
[Presentation] AAA+プロテアーゼLonの分子メカニズム2016
- Author(s)
  西井亘，新野陸子，寺田貴帆，白水美香子，村松知成，横山茂之 
- Organizer
  第39回日本分子生物学会年会・公募シンポジウム
- Place of Presentation
  パシフィコ横浜（横浜市）
- Year and Date
  2016-12-01
- Data Source
  KAKENHI-PROJECT-15K07019
[Presentation] A redox switch shapes the exit pore of Enterobacteriaceal Lon protease to facultatively regulate proteolysis2015
- Author(s)
  Nishii, W., Niino-Kukimoto, M, Terada, T., Shirouzu, M. Muramatsu, T., & Yokoyama, S. 
- Organizer
  9th General Meeting of the International Proteolysis Society
- Place of Presentation
  ペナン（マレーシア）
- Year and Date
  2015-10-06
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K07019
[Presentation] Production of hepatitis C virus NS2-3 protease by the Escherichia coli cell-free protein synthesis method and control of its activity2015
- Author(s)
  西井亘，松本武久，村松知成，横山茂之
- Organizer
  第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
- Place of Presentation
  神戸ポートアイランド（兵庫県神戸市）
- Year and Date
  2015-12-01
- Data Source
  KAKENHI-PROJECT-15K07019
[Presentation] アロステリックジスルフィド結合による細胞内プロテオリシスのレドックス制御2015
- Author(s)
  西井亘，横山茂之
- Organizer
  第15回日本蛋白質科学会年会公募型シンポジウム
- Place of Presentation
  あわぎんホール（徳島）
- Year and Date
  2015-06-26
- Data Source
  KAKENHI-PROJECT-15K07019
[Presentation] SARSコロナウィルス3CLプロテアーゼの基質認識メカニズム2007
- Author(s)
  金龍泰, 王宏飛, 竹本千重, 西井亘, 村松知成, 横山茂之
- Organizer
  第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
- Place of Presentation
  横浜
- Data Source
  KAKENHI-PROJECT-17770116
[Presentation] Cleavage mehcanisms of ATP-dependent Lon and HslVU proteases2007
- Author(s)
  Nishii, W., Muramatsu, T., Kim, Y.-T., Yokoyama, S. and Takahashi, K.
- Organizer
  5th General meeting of the International Proteolysis Society
- Place of Presentation
  Patras, Greece
- Data Source
  KAKENHI-PROJECT-17770116
[Presentation] SARSコロナウィルス3CLプロテアーゼプロ体の活性化メカニズム2007
- Author(s)
  金龍泰, 王宏飛, 竹本千重, 西井亘, 村松知成, 横山茂之
- Organizer
  第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
- Place of Presentation
  横浜
- Data Source
  KAKENHI-PROJECT-17770116
[Presentation] ATP依存性プロテアーゼLonおよびHslVUの基質分解機構2007
- Author(s)
  西井亘, 村松知成, 金龍泰, 横山茂之, 高橋健治
- Organizer
  第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
- Place of Presentation
  横浜
- Data Source
  KAKENHI-PROJECT-17770116

1. TAKAHASHI Kenji (70011533)

# of Collaborated Projects: 2 results

# of Collaborated Products: 0 results
2. 工藤佳久 (20080179)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
3. 中村健 (50227906)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
4. KOJIMA Masaki (90277252)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
5. INOUE Hideshi (20184765)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results

Nishii Wataru 西井 亘

西井 亘 ニシイ ワタル

NISHII Wataru 西井 亘

Research Projects

Research Products

Co-Researchers

Regulatory mechanisms of the AAA+ Lon proteasePrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Redox regulation of proteolysis in the Enterobacteriacea cellsPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

大腸菌ATP依存性プロテアーゼLonおよびHslVUの基質切断機構Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

ATP依存性プロテアーゼによる生理基質機能部位の優先切断機構Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

蛍光基質を用いた細胞内プロテオリシスの可視化解析Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

生細胞における蛋白質分解過程の可視化解析

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Structural, Functional and Physiological Characterization of Novel Brain-Gut Proteinases

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

[Journal Article] SARS-CoV 3CL protease cleaves its C-terminal autoprocessing site by novel subsite cooperativity2016

Author(s)

Journal Title

Data Source

[Journal Article] Substrate Specificities of Porcine and Bovine Enterpeptidases toward the Peptide Val-(Asp)4-Lys-Ile-Val-Gly and Its Analogs2008

Author(s)

Journal Title

Data Source

[Journal Article] The effects of mutations in the carboxyl-teminal region on the catalytic activity of Escherichi a coli signal peptidase I.2008

Author(s)

Journal Title

Data Source

[Journal Article] Inhibition and stabilization of Aspergilloglutamic peptidase by the propeptide : Identication of critical sequences and residues in the propeptide.2005

Author(s)

Journal Title

Data Source

[Journal Article] Cleavage mechanism of ATP-dependent Lon protease toward ribosomal S2 protein2005

Author(s)

Journal Title

Data Source

[Journal Article] Identification of arginine residues important for the activity of Eschericha coli signal peptidase I2004

Author(s)

Journal Title

Data Source

[Patent] ウィルスプロテアーゼ生産方法2007

Inventor(s)

Industrial Property Rights Holder

Industrial Property Number

Filing Date

Data Source

[Presentation] ダイナミックな構造変化を介したAAA+プロテアーゼLonの制御メカニズム2017

Author(s)

Nishii Wataru 西井亘

西井亘ニシイワタル

NISHII Wataru 西井亘