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Kurokawa Hirofumi  黒河 博文

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KUROKAWA Hirofumi  黒河 博文

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Researcher Number 80359546
Other IDs
Affiliation (Current) 2020: 東北大学, 多元物質科学研究所, 講師
Affiliation (based on the past Project Information) *help 2015 – 2019: 東北大学, 多元物質科学研究所, 講師
2012 – 2014: 東北大学, 医学(系)研究科(研究院), 講師
2009 – 2011: Tohoku University, 大学院・医学系研究科, 講師
2009: 東北大学, 多元物質科学研究所, 助手
2003 – 2006: 東北大学, 多元物質科学研究所, 助手
Review Section/Research Field
Principal Investigator
Applied biochemistry / Biological Sciences / Risk sciences of radiation/Chemicals / Medium-sized Section 38:Agricultural chemistry and related fields
Except Principal Investigator
Risk sciences of radiation/Chemicals / 環境保全 / 環境影響評価(含放射線生物学)
Keywords
Principal Investigator
酸化ストレス / タンパク質 / X線結晶構造解析 / 分子センサー / 結晶構造解析 / ヘムセンサー / ホスホジエステラーゼ / PASドメイン / ヘム / 毒性化学物質 … More / Keap1 / Keapl / シグナル伝達 / オートファジー / Nrf2 / センサータンパク質 / 結晶構造 / センサー … More
Except Principal Investigator
シグナル伝達 / 環境汚染物質 / 蛋白合成 / NO / ヘム / 転写因子 / 赤血球 / 分子スイッチ / センサー酵素 / cAMP / 一酸化窒素 / 遺伝子工学 / Sensor enzyme / Signal transduction / NO,ヘム / 時計遺伝子 / ヘム鉄 / 環境汚染 / 水銀 / ヘム制御酵素・蛋白質 / ガスセンサー / 蛋白質合成 / 体内時計 / DNA結合 / 水銀イオン / NOガス / PASドメイン / プロテインマイクロアレイ / 環境応答 / Nrf2 / Keap1 / 酸化ストレス / 低酸素 / エリスロポエチン / 発現制御 / 環境 / ストレス / 遺伝子 / ヘムセンサー / プロテイン・マイクロアレイ / タンパク-タンパク相互作用 / 酸化還元状態 / 蛋白-蛋白相互作用 / シグナル / シトクロムP450 / 環境ホルモン / 電子移動 / キメラ / 薬物代謝 / チトクロムP450 / Molecular switch / Heme sensor / Protein microarray / Protein-protein interaction / redox states / 蛋白質翻訳・合成 / NO合成酵素 / 生物時計 / センサー / 蛋白翻訳・合成 / 部位特異的変異 / 軸配位子 / キナーゼ / ポルフィリン / Heme / Protein translation / synthesis / NO synthase / Environmental pollutants / Circadian rhythms / Transcription factor Less
  • Research Projects

    (11 results)
  • Research Products

    (35 results)
  • Co-Researchers

    (13 People)
  •  食品成分に制御されるセンサータンパクの構造アンサンブルPrincipal InvestigatorOngoing

    • Principal Investigator
      黒河 博文
    • Project Period (FY)
      2018 – 2020
    • Research Category
      Grant-in-Aid for Challenging Research (Exploratory)
    • Review Section
      Medium-sized Section 38:Agricultural chemistry and related fields
    • Research Institution
      Tohoku University
  •  Three-dimensional Structure of Chemical Sensor Keap1Principal Investigator

    • Principal Investigator
      Kurokawa Hirofumi
    • Project Period (FY)
      2014 – 2017
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      Applied biochemistry
    • Research Institution
      Tohoku University
  •  酸化ストレスセンサーと選択的蛋白質分解系によるシグナリング複合体の構造と機能Principal Investigator

    • Principal Investigator
      黒河 博文
    • Project Period (FY)
      2013 – 2014
    • Research Category
      Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
    • Review Section
      Biological Sciences
    • Research Institution
      Tohoku University
  •  酸化ストレスセンサーKeap1と選択的蛋白質分解系による新規シグナリング複合体Principal Investigator

    • Principal Investigator
      黒河 博文
    • Project Period (FY)
      2011 – 2012
    • Research Category
      Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
    • Review Section
      Biological Sciences
    • Research Institution
      Tohoku University
  •  hree-dimensional Structure of Nrf2-Maf transcription factor complex mediating the induction of phase II detoxifying enzymesPrincipal Investigator

    • Principal Investigator
      KUROKAWA Hirofumi
    • Project Period (FY)
      2009 – 2011
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      Risk sciences of radiation/Chemicals
    • Research Institution
      Tohoku University
  •  Molecular Mechanisms of Adaptive Responses to Food and Oxygen

    • Principal Investigator
      YAMAMOTO Masayuki
    • Project Period (FY)
      2007 – 2011
    • Research Category
      Grant-in-Aid for Creative Scientific Research
    • Research Institution
      Tohoku University
  •  Destruction of the biological clock system by environmental contaminants : Crosstalk between heme, NO, protein synthesis and clock genes

    • Principal Investigator
      SHIMIZU Toru
    • Project Period (FY)
      2005 – 2009
    • Research Category
      Grant-in-Aid for Scientific Research (S)
    • Research Field
      Risk sciences of radiation/Chemicals
    • Research Institution
      Tohoku University
  •  レドックスをセンスする新規ヘム制御ホスホジエステラーゼの結晶構造Principal Investigator

    • Principal Investigator
      黒河 博文
    • Project Period (FY)
      2005 – 2006
    • Research Category
      Grant-in-Aid for Young Scientists (B)
    • Research Field
      Applied biochemistry
    • Research Institution
      Tohoku University
  •  蛋白質生合成を翻訳レベルで調節するヘム関連キナーゼのX線結晶構造解析Principal Investigator

    • Principal Investigator
      黒河 博文
    • Project Period (FY)
      2003 – 2004
    • Research Category
      Grant-in-Aid for Young Scientists (B)
    • Research Field
      Applied biochemistry
    • Research Institution
      Tohoku University
  •  Analyses of Porphyria Caused by Environmental Pollutants : Concerted Reaction and Tempo of Heme and Porphyrin Syntheses

    • Principal Investigator
      SHIMIZU Toru
    • Project Period (FY)
      2002 – 2004
    • Research Category
      Grant-in-Aid for Scientific Research (A)
    • Research Field
      環境影響評価(含放射線生物学)
    • Research Institution
      Tohoku University
  •  Construction of Environmental Biremediation Enzymes Whose Catalysis is Regulated by Light and Molecular Switches

    • Principal Investigator
      SHIMIZU Toru
    • Project Period (FY)
      2001 – 2004
    • Research Category
      Grant-in-Aid for Scientific Research (B)
    • Research Field
      環境保全
    • Research Institution
      Tohoku University

All 2019 2018 2012 2011 2010 2009 2008 2007 2006 2005 2004

All Journal Article Presentation

  • [Journal Article] 酸化ストレスセンサー分子KEAP1阻害剤2011

    • Author(s)
      黒河博文、山本雅之
    • Journal Title

      血管医学

      Volume: 12 Pages: 23-30

    • Data Source
      KAKENHI-PUBLICLY-23121502
  • [Journal Article] 酸化ストレスセンサー分子KEAP1阻害剤2011

    • Author(s)
      黒河博文、山本雅之
    • Journal Title

      血管医学

      Volume: 12 Pages: 23-30

    • Data Source
      KAKENHI-PROJECT-21510066
  • [Journal Article] The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keap12010

    • Author(s)
      Komatsu M, Kurokawa H, Waguri S, Taguchi K, Kobayashi A, Ichimura Y, Sou YS, Ueno I, Sakamoto A, Tong KI, Kim M, Nishito Y, Iemura S, Natsume T, Ueno T, Kominami E, Motohashi H, Tanaka K, and Yamamoto M
    • Journal Title

      Nature Cell Biol

      Volume: 12 Pages: 213-223

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19GS0312
  • [Journal Article] The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keap1.2010

    • Author(s)
      Komatsu, M., H.Kurokawa, et al.
    • Journal Title

      Nature Cell Biology 12

      Pages: 213-223

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-21510066
  • [Journal Article] The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keap12010

    • Author(s)
      Komatsu, M., Kurokawa, H., Waguri, S., Taguchi, K., Kobayashi, A., Ichimura, Y., Sou, Y. S., Ueno, I., Sakamoto, A., Tong, K. I., Kim, M., Nishito, Y., Iemura, S., Natsume, T., Ueno, T., Kominami, E., Motohashi, H., Tanaka, K., Yamamoto, M.
    • Journal Title

      Nature Cell Biol 12

      Pages: 213-223

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19GS0312
  • [Journal Article] Keap 1 homodimer is a forked-stem structure with two large spheres en closing the intervening, double glycine repeat, and C-terminal domains2010

    • Author(s)
      Ogura T, Tong KI, Mio K, Maruyama Y, Kurokawa H, Sato C, Yamamoto M.
    • Journal Title

      Proc Natl Acad Sci USA 107

      Pages: 2842-2847

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19GS0312
  • [Journal Article] Keap1 homodimer is a forked-stem structure with two large spheres enclosing the intervening, double glycine repeat, and C-terminal domains2010

    • Author(s)
      Ogura T, Tong KI, Mio K, Maruyama Y, Kurokawa H, Sato C, Yamamoto M.
    • Journal Title

      Proc Natl Acad Sci USA 285

      Pages: 5417-5427

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19GS0312
  • [Journal Article] Keap1 is a forked-stem dimer structure with two large spheres enclosing the intervening, double glycine repeat, and C-terminal domains.2010

    • Author(s)
      Ogura, T., Tong, K.I., Mio, K., Maruyama, Y., Kurokawa, H., Sato, C., Yamamoto, M.
    • Journal Title

      Proc Natl Acad Sci U S A 107

      Pages: 2842-2847

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-21510066
  • [Journal Article] Keap1 homodimer is a forked-stem structure with two large spheres enclosing the intervening, double glycine repeat, and C-terminal domains2010

    • Author(s)
      Ogura T, Tong KI, Mio K, Maruyama Y, Kurokawa H, Sato C, and Yamamoto M
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 285 Pages: 5417-5427

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19GS0312
  • [Journal Article] Keap1 is a forked-stem dimer structure with two large spheres enclosing the intervening, double glycine repeat, and C-terminal domains2010

    • Author(s)
      Ogura, T., Tong, K. I., Mio, K., Maruyama, Y., Kurokawa, H., Sato, C., and Yamamoto, M.
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 107巻 Pages: 2842-2847

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-21510066
  • [Journal Article] The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keap12010

    • Author(s)
      Komatsu, M., Kurokawa, H., Waguri, S., Taguchi, K., Kobayashi, A., Ichimura, Y., Sou, Y.S., Ueno, I., Sakamoto, A., Tong, K.I., Kim, M., Nishito, Y., Iemura, S., Natsume, T., Ueno, T., Kominami, E., Motohashi, H., Tanaka, K., Yamamoto, M
    • Journal Title

      Nat Cell Biol. 12

      Pages: 213-223

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19GS0312
  • [Journal Article] The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keap12010

    • Author(s)
      Komatsu, M., Kurokawa, H., Waguri, S., Taguchi, K., Kobayashi, A., Ichimura, Y., Sou, Y. S., Ueno, I., Sakamoto, A., Tong, K. I., Kim, M., Nishito, Y., Iemura, S., Natsume, T., Ueno, T., Kominami, E., Motohashi, H., Tanaka, K., and Yamamoto, M
    • Journal Title

      Nature Cell Biology

      Volume: 12巻 Pages: 213-223

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-21510066
  • [Journal Article] Structural basis of alternative DNA recognition by Maf transcription factors2009

    • Author(s)
      Kurokawa, H., Motohashi, H., Sueno, S., Kimura, M., Takagawa, H., Kanno, Y., Yamamoto, M., and Tanaka, T
    • Journal Title

      Molecular and Cellular Biology

      Volume: 29巻 Pages: 6232-6244

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-21510066
  • [Journal Article] Structural basis of alternative DNA recognition by Maf transcription factors2009

    • Author(s)
      Kurokawa, H., Motohashi, H., Sueno, S., Kimura, M., Takagawa, H., Kanno, Y., Yamamoto, M., Tanaka, T
    • Journal Title

      Molecular and Cellular Biology 29

      Pages: 6232-6244

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-21510066
  • [Journal Article] Structural basis of alternative DNA recognition by Maf transcription factors2009

    • Author(s)
      Kurokawa H, Motohashi H, Sueno S, Kimura M, Takagawa H, Kanno Y, Yamamoto M, Tanaka T.
    • Journal Title

      Mol.Cell.Biol. 29

      Pages: 6232-6244

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19GS0312
  • [Journal Article] Resonance Raman investigation of redox-induced structural changes of protein and heme in the sensor domain of Ec DOS protein2008

    • Author(s)
      S. F. El-Mashtoly, H. Takahashi, H. Kurokawa, A. Sato, T. Shimizu, T. Kitagawa
    • Journal Title

      J.Raman Spectrosc. 39

      Pages: 1614-1626

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-17101002
  • [Journal Article] Molecular basis distinguishing the DNA binding profile of NRF2-MAF heterodimer from that of MAF homodimer.2007

    • Author(s)
      Kimura M, Yamamoto T, Zhang J, Itoh K, Kyo M, Kamiya T, Aburatani H, Katsuoka F, Kurokawa H, Tanaka T, Motohashi H, and Yamamoto M.
    • Journal Title

      J Biol Chem. 282

      Pages: 33681-33690

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19GS0312
  • [Journal Article] Structure-function relationships of EcDOS, a heme-regulated phosphodiesterase from Escherichia coli2006

    • Author(s)
      Y. Sasakura, T. Yoshimura-Suzuki, H. Kurokawa, T. Shimizu
    • Journal Title

      Acc.Chem.Res. 39

      Pages: 37-43

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-17101002
  • [Journal Article] Characterization of heme-regulated eIF2 alpha kinase : Roles of the N-terminal domain in the oligomeric state, heme binding, catalysis, and inhibition2006

    • Author(s)
      M. Miksanova, J. Igarashi, M. Minami, I. Sagami, S. Yamauchi, H. Kurokawa, T. Shimizu
    • Journal Title

      Biochemistry 45

      Pages: 9894-9905

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-17101002
  • [Journal Article] Critical roles of Leu99 and Leu115 at the heme distal side in auto-oxidation and the redox potential of a heme-regulated phosphodiesterase from Escherichia coli2006

    • Author(s)
      N. Yokota, Y. Araki, H. Kurokawa, O. Ito, J. Igarashi, T. Shimizu
    • Journal Title

      FEBS J. 273

      Pages: 1210-1223

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-17101002
  • [Journal Article] Spectroscopic and DNA-binding characterization of the isolated heme-bound basic helix-loop-helix-PAS-A domain of neuronal PAS protein 2 (NPAS2), a transcription activator protein associated with circadian rhythms2006

    • Author(s)
      Y. Mukaiyama, T. Uchida, E. Sato, A. Sasaki, Y. Sato, J. Igarashi, H. Kurokawa, I. Sagami, T. Kitagawa, T. Shimizu
    • Journal Title

      FEBS J. 273

      Pages: 2528-2539

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-17101002
  • [Journal Article] Spectroscopic characterization of the isolated heme-bound PAS-B domain of neuronal PAS domain protein 2 associated with circadian rhythms2005

    • Author(s)
      R. Koudo, H. Kurokawa, E. Sato, J. Igarashi, T. Uchida, I. Sagami, T. Kitagawa, T. Shimizu
    • Journal Title

      FEBS J. 272

      Pages: 4153-4162

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-17101002
  • [Journal Article] A Redox-controlled Molecular Switch Revealed by the Crystal Structure of Bacterial Heme PAS Sensor2004

    • Author(s)
      H.Kurokawa
    • Journal Title

      Journal of Biological Chemistry 279(19)

      Pages: 20186-20193

    • Data Source
      KAKENHI-PROJECT-13558069
  • [Journal Article] A Redox-controlled Molecular Switch Revealed y the Crystal Structure of a Bacterial Heme PAS Sensor2004

    • Author(s)
      H.Kurokawa
    • Journal Title

      Journal of Biological Chemistry 279

      Pages: 20186-20193

    • Data Source
      KAKENHI-PROJECT-15780066
  • [Presentation] cis型プレニル基転移酵素の構造ダイナミクス2019

    • Author(s)
      黒河博文
    • Organizer
      第29回イソプレノイド研究会例会
    • Data Source
      KAKENHI-PROJECT-18K19161
  • [Presentation] 結晶構造解析によるcis型プレニル基転移酵素のダイナミクスと活性制御2018

    • Author(s)
      黒河博文、安保貴永、古山種俊
    • Organizer
      第28回イソプレノイド研究会例会
    • Data Source
      KAKENHI-PROJECT-18K19161
  • [Presentation] 発癌性物質や酸化ストレスに応答する生体防御系センサーの構造基盤2012

    • Author(s)
      黒河博文, 本橋ほづみ, 佐藤主税、山本雅之
    • Organizer
      平成23年度ターゲットタンパク研究プログラム公開シンポジウム
    • Place of Presentation
      学術総合センター
    • Year and Date
      2012-03-12
    • Data Source
      KAKENHI-PROJECT-21510066
  • [Presentation] 発癌性物質や酸化ストレスに応答する生体防御系センサーの構造基盤2011

    • Author(s)
      黒河博文, 本橋ほづみ, 佐藤主税、山本雅之
    • Organizer
      平成22年度ターゲットタンパク研究プログラム公開シンポジウム
    • Place of Presentation
      東京大学安田講堂
    • Year and Date
      2011-03-11
    • Data Source
      KAKENHI-PROJECT-21510066
  • [Presentation] 発癌性物質や酸化ストレスに応答する生体防御系センサーの構造基盤2010

    • Author(s)
      黒河博文、本橋ほづみ、佐藤主税、山本雅之
    • Organizer
      ターゲットタンパク研究プログラム平成22年度成果発表会
    • Place of Presentation
      東京
    • Year and Date
      2010-10-20
    • Data Source
      KAKENHI-PROJECT-19GS0312
  • [Presentation] Structural Basis of Sensor System for Cytoprotective Gene Expression Responding to Carcinogens and Oxidative Stress2009

    • Author(s)
      黒河博文
    • Organizer
      Cancer Science Institute of Singapore(CSI)-NMGCOE Joint Workshop
    • Place of Presentation
      Cancer Science Institute of Singapore
    • Year and Date
      2009-09-09
    • Data Source
      KAKENHI-PROJECT-19GS0312
  • [Presentation] 発癌性物質や酸化ストレスに応答する生体防御系センサーの構造基盤2009

    • Author(s)
      黒河博文
    • Organizer
      ターゲットタンパク研究プログラム 第3回全体交流会
    • Place of Presentation
      京都
    • Year and Date
      2009-08-31
    • Data Source
      KAKENHI-PROJECT-19GS0312
  • [Presentation] Structural basis of alternative DNA recognition by Maf transcription factors2009

    • Author(s)
      黒河博文
    • Organizer
      The 1st International Symposium "Challenge to Medical Innovation"
    • Place of Presentation
      仙台
    • Year and Date
      2009-12-07
    • Data Source
      KAKENHI-PROJECT-19GS0312
  • [Presentation] Crystal Structure of MafG in complex with its cognate DNA2008

    • Author(s)
      黒河博文, 他
    • Organizer
      The 21st NAITO conference on Nuclear Dynamics and RNA[I]
    • Place of Presentation
      山梨
    • Data Source
      KAKENHI-PROJECT-19GS0312
  • [Presentation] Maf転写因子-DNA複合体の結晶構造2008

    • Author(s)
      黒河博文, 他
    • Organizer
      第5回特定領域研究「生体超分子の構造形成と機能制御の原子機構」公開シンポジウム
    • Place of Presentation
      つくば
    • Data Source
      KAKENHI-PROJECT-19GS0312
  • [Presentation] Role of the N-terminal Domain in the Structure and Catalysis of Heme-regualted Eukaryotic Initiation Factor 2α Kinase, a Heme-sensor Enzyme2006

    • Author(s)
      Marketa Miksanova, Hirofumi Kurokawa, Jotaro Igarashi, Toru Shimizu
    • Organizer
      Biochemistry and Molecular Biology of Sensor Enzymes and Proteins : Satellite Meeting of the 20th IUBMB International Congress of Biochemistry and Molecular Biology
    • Place of Presentation
      Japan, Sendai
    • Data Source
      KAKENHI-PROJECT-17101002
  • 1.  SHIMIZU Toru (40118956)
    # of Collaborated Projects: 3 results
    # of Collaborated Products: 7 results
  • 2.  IGARASHI Jotaro (80375162)
    # of Collaborated Projects: 3 results
    # of Collaborated Products: 5 results
  • 3.  佐上 郁子 (10143033)
    # of Collaborated Projects: 2 results
    # of Collaborated Products: 0 results
  • 4.  TANAKA Atsunari (20431471)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 5.  YAMAMOTO Masayuki (50166823)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 9 results
  • 6.  KATSUOKA Fumiki (30447255)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 1 results
  • 7.  MINEGISHI Naoko (40271895)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 8.  MOTOHASHI Hozumi (00282351)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 6 results
  • 9.  SUZUKI Norio (20447254)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 10.  MORIGUCHI Takashi (10447253)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 11.  SUZUKI Takafumi (70508308)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 12.  TAGUCHI Keiko (20466527)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 3 results
  • 13.  松井 敏高 (90323120)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results

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