OHTSUKA MASATO 大塚正人

… Alternative Names

OHTSUKA Masato 大塚正人

大塚正人オオツカマサト

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Researcher Number	90372945
Other IDs	https://orcid.org/0000-0002-6952-4238
Affiliation (Current)	2025: 東海大学, 医学部, 教授
Affiliation (based on the past Project Information) *help	2019 – 2024: 東海大学, 医学部, 教授 2014 – 2018: 東海大学, 医学部, 准教授 2016: 東海大学, 医学部, 講師 2016: 東海大学, 歯学部, 准教授 2010 – 2013: Tokai University, 医学部, 講師 … More 2009: Tokai University, 総合医学研究所, 講師 2008: Tokai University, 総合医学研究所, 助教 2007: 東海大学, 医学部, 助教 2004 – 2005: 東海大学, 医学部, 助手 Less
Review Section/Research Field	Principal Investigator Laboratory animal science / Laboratory animal science / Basic Section 42040:Laboratory animal science-related / Medium-sized Section 42:Veterinary medical science, animal science, and related fields / Medium-sized Section 50:Oncology and related fields / Applied genomics Except Principal Investigator Basic Section 57050:Prosthodontics-related / Basic Section 59040:Nutrition science and health science-related / Kidney internal medicine / Medium-sized Section 57:Oral science and related fields … More / Basic Section 50020:Tumor diagnostics and therapeutics-related / Basic Section 58020:Hygiene and public health-related: including laboratory approach / Medium-sized Section 59:Sports sciences, physical education, health sciences, and related fields / Basic Section 52020:Neurology-related / Basic Section 42040:Laboratory animal science-related / General physiology / Surgical dentistry / Neurochemistry/Neuropharmacology / Hematology / Prosthodontics/ Dental materials science and / Functional basic dentistry / Integrative animal science / Risk sciences of radiation/Chemicals / Ophthalmology / Dental engineering/Regenerative dentistry / Biological Sciences / Applied genomics / Human genetics Less
Keywords	Principal Investigator ノックダウン / 遺伝子治療 / ゲノム編集 / トランスジェニックマウス / 発生工学 / イントロン / 人工miRNA / マウス / トランスジェニック / PITT … More / Cre-loxP / 顕微注入法 / 糖尿病 / SEND / mRNA転送 / ウイルス様粒子 / 白血病 / マウスモデル / miRNA / インスリン抵抗性 / 生活習慣病 / ハイドロダイナミクス法 / piggyBac / 白内障 / CRISPR/Cas9 / i-GONAD / GONAD / in vivo / エレクトロポレーション / CRISPR / C57BL/6N / ターゲットトランスジェネシス / 組織特異的プロモーター / PITT法 / Rosa26 / PhiC31 / 顕微注入 / 部位特異的遺伝子挿入 / コンディショナル / コンディショナル発現 / 疾患感受性遺伝子 / Cre-loxpシステム / Rosa26遺伝子座位 / 蛍光遺伝子 / RMCE法 / Cre-loxPシステム / haplotype / ET cloning / homologous recombination / Bacterial Artificial Chromosome(BAC) / RMCE / recombineering / ES cell / chosen locus / single copy Tg / association study / Transgenic mouse / gene targeting … More Except Principal Investigator ゲノム編集 / 薬剤関連顎骨壊死 / デノスマブ / 転写因子 / 幹細胞 / マクロファージ / 心筋症 / 細胞療法 / DRONJ / BRONJ / ビスホスホネート製剤 / 次世代シーケンス解析 / ゲノム編集マウス / 全遺伝子解析 / 老化 / ポドサイト / 動物モデル / 病因 / 細胞治療 / ビスフォスフォネート製剤 / 遺伝子 / 創傷治癒 / 遺伝子改変マウス / 発生工学 / ホーミング / 間葉系幹細胞 / 疾患モデルマウス / 相関解析 / 骨配向性 / 難治性疾患 / Osteomacs / 慢性疾患 / ハイドロダイナミック遺伝子導入法 / 難治癌 / in vivo / 遺伝子治療 / 学習行動 / バイオマーカー / THAラット / 病態形成機構 / 極性変化 / 1細胞解析 / 階層構造 / MRONJ / 全ゲノム / メチル化 / 空間トランスクリプトーム / 非コードDNA / DUX4 / FSHD / 筋ジストロフィー / 分泌小胞 / エクソソーム / ゲノム解析 / 拡張型心筋症 / in situ遺伝子導入 / in situ 遺伝子導入 / ソノポーレーション / 経胎盤的遺伝子導入（TPGD） / 酸塩基調節 / 腎 / 低リン食 / ペンドリン / 間在細胞type-B / 腎集合管 / アルカリ分泌 / 集合管 / CaSR / カルシウム感知受容体 / 細胞移植 / 免疫病理 / 分子標的治療法 / ロモソズマブ / 分子標的治療 / リンパ管 / 治癒関連分子 / 病因関連分子 / 薬剤関連顎骨壊死（MRONJ） / 血管新生抑制薬 / 抗RANKL抗体製剤 / マウス / 次世代シーケンサー / 排卵 / 卵巣 / 不妊 / レポーター / ネフリン / 腎臓学 / レポーターアッセイ / スクリーニング / 化合物ライブラリー / 糸球体 / 腎臓内科学 / 腎発生 / Foxc2 / Foxc1 / ポドサイト傷害 / 尿タンパク / フォークヘッド転写因子 / フォークヘッド / 糸球体硬化 / 脂肪組織由来幹細胞 / 抗RANKL抗体 / 顎骨壊死 / 抗癌剤 / 抗RANKL抗体製剤関連顎骨壊死 / ビスフォスフォネート製剤関連顎骨壊死 / 治療法 / 非培養SVF細胞 / 骨吸収抑制薬関連顎骨壊死 / 脳神経疾患 / 発現制御 / 神経科学 / 3'UTR / 神経細胞 / RNA結合タンパク質 / 選択的スプライシング / 血液免疫学 / miRNA / 細胞運命 / EBF1 / B細胞分化 / microRNA / 細胞分化 / 歯肉上皮細胞 / 陽極酸化・水熱処理チタン / 純チタン / 口腔インプラント / 骨髄由来間葉系幹細胞 / 歯学 / シグナル伝達 / ホーミング機構 / 歯髄幹細胞 / 表面・界面物性 / 口腔インプラント表面性状 / 傾斜機能型ナノハイブリッドインプラント / 歯科補綴学 / CCR2 / MSC / 口腔癌細胞 / M2マクロファージ / フィブロサイト / 口腔癌 / 接着因子 / 共培養 / 骨髄由来細胞 / M2-マクロファージ / マクロファージコロニー刺激因子 / 接着 / 免疫抑制 / ニッチ / 癌細胞 / 遺伝子導入 / 化学物質過敏症 / OPIDN / モデルマウス / 神経障害エステラーゼ / 遺伝子改変 / トランスジェニックマウス / CRISPR / ROSA26 / esterase / PNPLA6 gene / 遺伝子発現 / 有機リン / PNPLA6 / 遺伝子操作マウス / 疾患感受性遺伝子 / シックハウス症候群 / 自己免疫疾患 / 眼遺伝学 / 眼免疫学 / 臓器幹細胞 / tdTomato / 前駆細胞 / 分化 / 増殖 / 蛍光トレース / ジェノタイピング / リシークエンシング / ゲノム / データベース / 多型 / 疾患感受性 / ゲノタイピング / ゲノム多様性 / 次世代シークエンサー / HLA / エンドサイトーシス / 神経伝達物質 / シナプス小胞 / ゲノムワイド相関解析(GWAS) / 酵母ツーハイブリッド法 / シンタキシン / シナプトガミン / NETO / シナプトタグミン / コンタクチン / 感受性遺伝子 / GWAS / ネットワーク解析 / マイクロサテライト / 摂食障害 / 巨大DNA領域 / II型制限酵素 / 微量DNA / 相同組換え / 血液 / BAC / 疾患感受性領域 / TAR法 / 制限酵素 / ETクローニング / DNA抽出 Less

DUX4-flによる非遺伝子領域からの翻訳に着目したFSHDの病態解明
- Principal Investigator
  
  三橋弘明
- Project Period (FY)
  2024 – 2026
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 52020:Neurology-related
- Research Institution
  Tokai University
Analysis of genomic DNA in aging heart
- Principal Investigator
  
  水上洋一
- Project Period (FY)
  2024 – 2027
- Research Category
  
  Grant-in-Aid for Scientific Research (A)
- Review Section
  
  Medium-sized Section 59:Sports sciences, physical education, health sciences, and related fields
- Research Institution
  Yamaguchi University
内在性分子で構築されたウイルス様粒子による細胞間mRNA転送の特性の解明と応用Principal Investigator
- Principal Investigator
  
  大塚正人
- Project Period (FY)
  2023 – 2025
- Research Category
  
  Grant-in-Aid for Challenging Research (Exploratory)
- Review Section
  
  Medium-sized Section 42:Veterinary medical science, animal science, and related fields
- Research Institution
  Tokai University
Development of Organ- and Cell-specific In Vivo Genome Editing Technique as a Novel Approach for Cancer Gene Therapy
- Principal Investigator
  
  上村顕也
- Project Period (FY)
  2023 – 2026
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 50020:Tumor diagnostics and therapeutics-related
- Research Institution
  Niigata University
Determination of chronic or intractable mechanisms induced by low bone quality with osteomacs and bone marrow derived stem cells interaction
- Principal Investigator
  
  黒嶋伸一郎
- Project Period (FY)
  2023 – 2025
- Research Category
  
  Grant-in-Aid for Challenging Research (Exploratory)
- Review Section
  
  Medium-sized Section 57:Oral science and related fields
- Research Institution
  Nagasaki University
Understanding of pathophysiology and development of treatment strategy for MRONJ based on the hierarchical structure of macrophages
- Principal Investigator
  
  黒嶋伸一郎
- Project Period (FY)
  2022 – 2025
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 57050:Prosthodontics-related
- Research Institution
  Hokkaido University
  Nagasaki University
Establishment of a next-generation impact assessment tool for low-concentration chemical exposure using THA rats
- Principal Investigator
  
  遠藤整
- Project Period (FY)
  2022 – 2025
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 58020:Hygiene and public health-related: including laboratory approach
- Research Institution
  Tokai University
Whlole genome analysis in exosome from dilated cardiomyopathy occurred by aging
- Principal Investigator
  
  Mizukami Yoichi
- Project Period (FY)
  2021 – 2023
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 59040:Nutrition science and health science-related
- Research Institution
  Yamaguchi University
Establishment of gene expression control system by modification of introns and its application to analysis of diabetes-related gene functionsPrincipal Investigator
- Principal Investigator
  
  大塚正人
- Project Period (FY)
  2021 – 2024
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 42040:Laboratory animal science-related
- Research Institution
  Tokai University
Development of novel genome editing tool for gene therapy and its application to cancer therapyPrincipal Investigator
- Principal Investigator
  
  Ohtsuka Masato
- Project Period (FY)
  2020 – 2021
- Research Category
  
  Grant-in-Aid for Challenging Research (Exploratory)
- Review Section
  
  Medium-sized Section 50:Oncology and related fields
- Research Institution
  Tokai University
Generation of gene modified mice using in situ genome editing system without microinjection
- Principal Investigator
  
  Nakamura Shingo
- Project Period (FY)
  2019 – 2021
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 42040:Laboratory animal science-related
- Research Institution
  National Defense Medical College
Identification of infertile genes in aging mice using genome editing method
- Principal Investigator
  
  Mizukami Yoichi
- Project Period (FY)
  2018 – 2022
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 59040:Nutrition science and health science-related
- Research Institution
  Yamaguchi University
Molecular targeted therapy for MRONJ
- Principal Investigator
  
  KUROSHIMA Shinichiro
- Project Period (FY)
  2018 – 2021
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 57050:Prosthodontics-related
- Research Institution
  Nagasaki University
Development of novel gene therapy strategy by insertion of artificial miRNA sequence into intronic region of endogenous gene(Fostering Joint International Research)Principal Investigator
- Principal Investigator
  
  OHTSUKA Masato
- Project Period (FY)
  2017 – 2019
- Research Category
  
  Fund for the Promotion of Joint International Research (Fostering Joint International Research)
- Research Field
  
  Laboratory animal science
- Research Institution
  Tokai University
Identification of miRNA which rescues EBF1 deficiency indispensable for B cell lineage commitment
- Principal Investigator
  
  KOTANI Ai
- Project Period (FY)
  2017 – 2019
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  Hematology
- Research Institution
  Tokai University
The proposal of novel experimental system using original knockdown methods in micePrincipal Investigator
- Principal Investigator
  
  OHTSUKA Masato
- Project Period (FY)
  2016 – 2018
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  Laboratory animal science
- Research Institution
  Tokai University
Development of microinjection-independent animal genome manipulation method
- Principal Investigator
  
  NAKAMURA Shingo
- Project Period (FY)
  2016 – 2018
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  Integrative animal science
- Research Institution
  National Defense Medical College
Screening of novel podocyte protective reagents using nephrin reporter mice
- Principal Investigator
  
  Mori Kiyoshi
- Project Period (FY)
  2016 – 2017
- Research Category
  
  Grant-in-Aid for Challenging Exploratory Research
- Research Field
  
  Kidney internal medicine
- Research Institution
  University of Shizuoka
Role of calcium sensing receptor in Kidney Type B Intercalated Cells
- Principal Investigator
  
  Yasuoka Yukiko
- Project Period (FY)
  2016 – 2018
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  General physiology
- Research Institution
  Kitasato University
Development of novel method for generation of genome editing mice without ex vivo handling of embryosPrincipal Investigator
- Principal Investigator
  
  OHTSUKA Masato
- Project Period (FY)
  2015 – 2017
- Research Category
  
  Grant-in-Aid for Challenging Exploratory Research
- Research Field
  
  Laboratory animal science
- Research Institution
  Tokai University
The RNA-based mechanism and function underlying molecular diversity in the nervous system
- Principal Investigator
  
  IIJIMA Takatoshi
- Project Period (FY)
  2015 – 2017
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  Neurochemistry/Neuropharmacology
- Research Institution
  Tokai University
Transplantation effect of ADSC and SVF cells on ARONJ-like lesions in mice
- Principal Investigator
  
  KUROSHIMA Shinichiro
- Project Period (FY)
  2015 – 2017
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Surgical dentistry
- Research Institution
  Nagasaki University
Bone marrow-derived fibrocytes and/or macrophages interact with oral cancer cells and stimulate the malignant progression of cancer cells
- Principal Investigator
  
  Kyakumoto Seiko
- Project Period (FY)
  2014 – 2016
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Functional basic dentistry
- Research Institution
  Iwate Medical University
Development and verification of the next generation transgenesis toolsPrincipal Investigator
- Principal Investigator
  
  OHTSUKA Masato
- Project Period (FY)
  2013 – 2015
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  Laboratory animal science
- Research Institution
  Tokai University
Analysis for homing mechanism of mesenchymal stem cells on functionally graded nanohybrid titanium dental implant
- Principal Investigator
  
  Takebe Jun
- Project Period (FY)
  2013 – 2015
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Prosthodontics/ Dental materials science and
- Research Institution
  Aichi Gakuin University
  Iwate Medical University
Foxc1/2 transcription factors and glomerulosclerosis
- Principal Investigator
  
  MOTOJIMA Masaru
- Project Period (FY)
  2013 – 2017
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Kidney internal medicine
- Research Institution
  Tokai University
Analysis for the function of the candidate gene of Sick House Syndrome and development of disease model mouse
- Principal Investigator
  
  KIMURA Minoru
- Project Period (FY)
  2012 – 2014
- Research Category
  
  Grant-in-Aid for Challenging Exploratory Research
- Research Field
  
  Risk sciences of radiation/Chemicals
- Research Institution
  Tokai University
Elucidation of molecular mechanisms underlying homing and mobilization of bone marrow-derived mesenchymal stem cells into oral and maxillofacial tissues
- Principal Investigator
  
  ISHISAKI AKIRA
- Project Period (FY)
  2011 – 2013
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Dental engineering/Regenerative dentistry
- Research Institution
  Iwate Medical University
Development of conditional expression system in mice generated by site-specific gene integration methodPrincipal Investigator
- Principal Investigator
  
  OHTSUKA Masato
- Project Period (FY)
  2011 – 2012
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Laboratory animal science
- Research Institution
  Tokai University
Research for the molecular mechanism of Behcet's disease
- Principal Investigator
  
  KAWAGOE Tatsukata
- Project Period (FY)
  2011 – 2012
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Ophthalmology
- Research Institution
  Yokohama City University
HLA genome diversity analyses by resequencing
- Principal Investigator
  
  SHIINA Takashi
- Project Period (FY)
  2010 – 2014
- Research Category
  
  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
- Review Section
  
  Biological Sciences
- Research Institution
  Tokai University
Development and application of novel transgenic technology by in vivo RMCEPrincipal Investigator
- Principal Investigator
  
  OHTSUKA Masato
- Project Period (FY)
  2008 – 2010
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Laboratory animal science
- Research Institution
  Tokai University
Genome-wide identification of genes for anorexia and their cascade analysis on disease development by functional study
- Principal Investigator
  
  INOKO Hidetoshi
- Project Period (FY)
  2007 – 2011
- Research Category
  
  Grant-in-Aid for Scientific Research (S)
- Research Field
  
  Applied genomics
- Research Institution
  Tokai University
疾患感受性ハプロタイプを検証する為の新規BACトランスジェニック手法の開発Principal Investigator
- Principal Investigator
  
  大塚正人
- Project Period (FY)
  2004 – 2005
- Research Category
  
  Grant-in-Aid for Exploratory Research
- Research Field
  
  Applied genomics
- Research Institution
  Tokai University
ヒト少量血液からの対象ゲノム領域クローニング新手法の開発
- Principal Investigator
  
  木村穣
- Project Period (FY)
  2004 – 2005
- Research Category
  
  Grant-in-Aid for Exploratory Research
- Research Field
  
  Human genetics
- Research Institution
  Tokai University

[Book] New Insights into Theriogenology2018
- Author(s)
  Sato Masahiro、Ohtsuka Masato、Nakamura Shingo
- Publisher
  IntechOpen
- ISBN
  9781789847734
- Data Source
  KAKENHI-PROJECT-16H05049
[Book] PITT法によるターゲットトランスジェニックマウス作製　in 動物／疾患モデルの作製技術・病態解析・評価手法2017
- Author(s)
  大塚正人、三浦浩美
- Total Pages
  428
- Publisher
  技術情報協会
- ISBN
  9784861046650
- Data Source
  KAKENHI-PROJECT-16H04685
[Book] CRISPR/Cas9 and the Paradigm Shift in Mouse Genome Manipulation Technologies. In: Turksen K (ed) Genome editing2016
- Author(s)
  Gurumurthy C.B., Quadros R., Sato M., Mashimo T., Lloyd K.C.K. and Ohtsuka M
- Total Pages
  13
- Publisher
  Springer International Publishing
- Data Source
  KAKENHI-PROJECT-15K14371
[Book] 遺伝子改変法①部位特異的組換え酵素システム．ES iPS細胞実験スタンダード2014
- Author(s)
  大塚正人、角田茂
- Publisher
  羊土社
- Data Source
  KAKENHI-PROJECT-25290035
[Book] Development and recent progress in the pronuclear injection-based targeted transgenesis method by site-specific recombination in mice. in Proceedings of the Genetic Transformation Technologies (plants and animals)2013
- Author(s)
  Ohtsuka M.
- Publisher
  MEDIMOND
- Data Source
  KAKENHI-PROJECT-25290035
[Book] a medaka mutant exhibiting a mirror-image pattern duplication of the dorsal-ventral axis. in Medaka: Model for Organogesis, Human Diseases and Evolution2011
- Author(s)
  Ohtsuka M., Takeda H., and Shimada A.
- Total Pages
  15
- Publisher
  Springer press
- Data Source
  KAKENHI-PROJECT-23700514
[Book] Human Diseases and Evolution. Chapter 132011
- Author(s)
  OhtsukaM., TakedaH., and Shimada A
- Publisher
  Double anal fin (Da): a medaka mutant exhibiting a mirror-image pattern duplication of the dorsal-ventral axis. in Medaka: Model for Organogesi
- Data Source
  KAKENHI-PROJECT-23700514
[Journal Article] Designing and generating a mouse model: frequently asked questions2021
- Author(s)
  Gurumurthy Channabasavaiah B.、Saunders Thomas L.、Ohtsuka Masato
- Journal Title
  
  The Journal of Biomedical Research
  
  Volume: 35 Issue: 2 Pages: 76-76
- DOI
  10.7555/jbr.35.20200197
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18K11128
[Journal Article] Novel Reporter Mouse Models Useful for Evaluating In Vivo Somatic Cell Gene Editing and for Optimization of Methods of Delivering Genome Editing Tools.2021
- Author(s)
  Miura H., Imafuku J., Kurosaki A., Sato M., Ma Y., Zhang G., Mizutani A., Kamimura K., Gurumurthy CB., Liu D., Ohtsuka M.
- Journal Title
  
  Molecular Therapy-Nucleic Acids.
  
  Volume: 24 Pages: 325-336
- DOI
  10.1016/j.omtn.2021.03.003
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18K06040, KAKENHI-PROJECT-18K11128, KAKENHI-PROJECT-20K08379, KAKENHI-PROJECT-19K06372
[Journal Article] Modification of i-GONAD Suitable for Production of Genome-Edited C57BL/6 Inbred Mouse Strain2020
- Author(s)
  Kobayashi Yukari、Aoshima Takuya、Ito Ryota、Shinmura Ryota、Ohtsuka Masato、Akasaka Eri、Sato Masahiro、Takabayashi Shuji
- Journal Title
  
  Cells
  
  Volume: 9 Issue: 4 Pages: 957-957
- DOI
  10.3390/cells9040957
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18K11128, KAKENHI-PROJECT-19K06372
[Journal Article] Genetically modified mouse models to help fight COVID-192020
- Author(s)
  Gurumurthy Channabasavaiah B.、Quadros Rolen M.、Richardson Guy P.、Poluektova Larisa Y.、Mansour Suzanne L.、Ohtsuka Masato
- Journal Title
  
  Nature Protocols
  
  Volume: 15 Issue: 12 Pages: 3777-3787
- DOI
  10.1038/s41596-020-00403-2
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18K11128
[Journal Article] Sequential i-GONAD: An Improved In Vivo Technique for CRISPR/Cas9-Based Genetic Manipulations in Mice2020
- Author(s)
  Sato Masahiro、Miyagasako Rico、Takabayashi Shuji、Ohtsuka Masato、Hatada Izuho、Horii Takuro
- Journal Title
  
  Cells
  
  Volume: 9 Issue: 3 Pages: 546-546
- DOI
  10.3390/cells9030546
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18K11128, KAKENHI-PROJECT-19K06372, KAKENHI-PROJECT-19H03143
[Journal Article] Effect of Diphtheria Toxin-Based Gene Therapy for Hepatocellular Carcinoma2020
- Author(s)
  Kamimura Kenya、Yokoo Takeshi、Abe Hiroyuki、Sakai Norihiro、Nagoya Takuro、Kobayashi Yuji、Ohtsuka Masato、Miura Hiromi、Sakamaki Akira、Kamimura Hiroteru、Miyamura Norio、Nishina Hiroshi、Terai Shuji
- Journal Title
  
  Cancers
  
  Volume: 12 Issue: 2 Pages: 472-472
- DOI
  10.3390/cancers12020472
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-17K09408, KAKENHI-PROJECT-18K19392, KAKENHI-PROJECT-18K19537, KAKENHI-PROJECT-20K08379, KAKENHI-PROJECT-20H03381, KAKENHI-PROJECT-16KK0189
[Journal Article] Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation2019
- Author(s)
  Gurumurthy Channabasavaiah B.、O’Brien Aidan R.、Quadros Rolen M.、Adams John、Alcaide Pilar、Ayabe Shinya、Ballard Johnathan、Batra Surinder K.、Beauchamp Marie-Claude、Becker Kathleen A.、Bernas Guillaume、Ohtsuka Masato他
- Journal Title
  
  Genome Biology
  
  Volume: 20 Issue: 1 Pages: 171-171
- DOI
  10.1186/s13059-019-1776-2
- NAID
  120007127906
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18K11128, KAKENHI-PROJECT-19K09031, KAKENHI-PROJECT-18K15255, KAKENHI-PROJECT-19K16025, KAKENHI-PROJECT-18KK0251, KAKENHI-PROJECT-18H03974, KAKENHI-PROJECT-17H04282, KAKENHI-PROJECT-17KT0126
[Journal Article] SAM68-Specific Splicing Is Required for Proper Selection of Alternative 3' UTR Isoforms in the Nervous System2019
- Author(s)
  Iijima Y, Tanaka M, Suzuki S, Hauser D, Tanaka M, Okada C, Ito M, Ayukawa N, Sato Y, Ohtsuka M, Scheiffele P, Iijima T.
- Journal Title
  
  iScience
  
  Volume: 22 Pages: 318-335
- DOI
  10.1016/j.isci.2019.11.028
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18K11128, KAKENHI-PROJECT-19H00299
[Journal Article] Creation of CRISPR-based germline-genome-engineered mice without ex vivo handling of zygotes by i-GONAD2019
- Author(s)
  Gurumurthy Channabasavaiah B.、Sato Masahiro、Nakamura Ayaka、Inui Masafumi、Kawano Natsuko、Islam Md Atiqul、Ogiwara Sanae、Takabayashi Shuji、Matsuyama Makoto、Nakagawa Shinichi、Miura Hiromi、Ohtsuka Masato
- Journal Title
  
  Nature Protocols
  
  Volume: 14 Issue: 8 Pages: 2452-2482
- DOI
  10.1038/s41596-019-0187-x
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18K11128, KAKENHI-PROJECT-19K06372, KAKENHI-PROJECT-19K06474
[Journal Article] i-GONAD: A method for generating genome‐edited animals without ex vivo handling of embryos2019
- Author(s)
  Ohtsuka Masato、Sato Masahiro
- Journal Title
  
  Development, Growth & Differentiation
  
  Volume: 61 Issue: 5 Pages: 306-315
- DOI
  10.1111/dgd.12620
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18K11128, KAKENHI-PROJECT-19K06372
[Journal Article] i-GONAD (improved genome-editing via oviductal nucleic acids delivery), a convenient in vivo tool to produce genome-edited rats2018
- Author(s)
  Takabayashi Shuji、Aoshima Takuya、Kabashima Katsuya、Aoto Kazushi、Ohtsuka Masato、Sato Masahiro
- Journal Title
  
  Scientific Reports
  
  Volume: 8 Issue: 1 Pages: 12059-12059
- DOI
  10.1038/s41598-018-30137-x
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-17K08513, KAKENHI-PROJECT-18K06028, KAKENHI-PROJECT-18K11128
[Journal Article] Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors2018
- Author(s)
  Miura H, Quadros RM, Gurumurthy CB, Ohtsuka M
- Journal Title
  
  Nat Protoc.
  
  Volume: 13 Issue: 1 Pages: 195-215
- DOI
  10.1038/nprot.2017.153
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16H04685, KAKENHI-PROJECT-16K18821
[Journal Article] Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line2018
- Author(s)
  Sakurai Takayuki、Kamiyoshi Akiko、Ohtsuka Masato、Gurumurthy Channabasavaiah B.、Sato Masahiro、Shindo Takayuki
- Journal Title
  
  Methods in Molecular Biology
  
  Volume: 1874 Pages: 257-271
- DOI
  10.1007/978-1-4939-8831-0_15
- ISBN
  9781493988303, 9781493988310
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18K11128, KAKENHI-PROJECT-16H04685
[Journal Article] Intravenous Delivery of piggyBac Transposons as a Useful Tool for Liver-Specific Gene-Switching2018
- Author(s)
  Nakamura Shingo、Ishihara Masayuki、Watanabe Satoshi、Ando Naoko、Ohtsuka Masato、Sato Masahiro
- Journal Title
  
  International Journal of Molecular Sciences
  
  Volume: 19 Issue: 11 Pages: 3452-3452
- DOI
  10.3390/ijms19113452
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18K11128, KAKENHI-PROJECT-16H04685, KAKENHI-PROJECT-16H05049, KAKENHI-PROJECT-16K15063
[Journal Article] Timing of CRISPR/Cas9-related mRNA microinjection after activation as an important factor affecting genome editing efficiency in porcine oocytes2018
- Author(s)
  Sato M, Maeda K, Koriyama M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai, T, Watanabe S, Miyoshi K
- Journal Title
  
  Theriogenology
  
  Volume: 108 Pages: 29-38
- DOI
  10.1016/j.theriogenology.2017.11.030
- NAID
  120007099790
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-16K08085, KAKENHI-PROJECT-16K15063, KAKENHI-PROJECT-16H05049, KAKENHI-PROJECT-16K15233, KAKENHI-PROJECT-18K09839, KAKENHI-PROJECT-16H05176, KAKENHI-PROJECT-16K11810
[Journal Article] Cell?cell interactions between monocytes/macrophages and synoviocyte-like cells promote inflammatory cell infiltration mediated by augmentation of MCP-1 production in temporomandibular joint2018
- Author(s)
  Ibi Miho、Horie Sawa、Kyakumoto Seiko、Chosa Naoyuki、Yoshida Mariko、Kamo Masaharu、Ohtsuka Masato、Ishisaki Akira
- Journal Title
  
  Bioscience Reports
  
  Volume: 38 Issue: 2 Pages: 20171217-20171217
- DOI
  10.1042/bsr20171217
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-16K11654, KAKENHI-PROJECT-17K11851, KAKENHI-PROJECT-18K11128, KAKENHI-PROJECT-17K19774, KAKENHI-PROJECT-26293426
[Journal Article] Synchrotron radiation microtomography of brain hemisphere and spinal cord of a mouse model of multiple sclerosis revealed a correlation between capillary dilation and clinical score2018
- Author(s)
  Saiga Rino、Hoshino Masato、Takeuchi Akihisa、Uesugi Kentaro、Naitou Katsuko、Kamijo Akemi、Kawabe Noboru、Ohtsuka Masato、Takizawa Shunya、Mizutani Ryuta
- Journal Title
  
  Journal of Comparative Neurology
  
  Volume: - Issue: 13 Pages: 2091-2100
- DOI
  10.1002/cne.24544
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18K11128
[Journal Article] In vivo genome editing targeted towards the female reproductive system2018
- Author(s)
  Sato Masahiro、Ohtsuka Masato、Nakamura Shingo、Sakurai Takayuki、Watanabe Satoshi、Gurumurthy Channabasavaiah B.
- Journal Title
  
  Archives of Pharmacal Research
  
  Volume: 41 Issue: 9 Pages: 898-910
- DOI
  10.1007/s12272-018-1053-z
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18K11128, KAKENHI-PROJECT-16H05049
[Journal Article] Successful production of genome-edited rats by the rGONAD method2018
- Author(s)
  Kobayashi T, Namba M, Koyano T, Fukushima M, Sato M, Ohtsuka M, Matsuyama M.
- Journal Title
  
  BMC Biotechnol
  
  Volume: 18 Issue: 1 Pages: 19-19
- DOI
  10.1186/s12896-018-0430-5
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-15K14371, KAKENHI-PROJECT-18K11128
[Journal Article] i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases2018
- Author(s)
  Ohtsuka Masato、Sato Masahiro、Miura Hiromi、Takabayashi Shuji、Matsuyama Makoto、Koyano Takayuki、Arifin Naomi、Nakamura Shingo、Wada Kenta、Gurumurthy Channabasavaiah B.
- Journal Title
  
  Genome Biology
  
  Volume: 19 Issue: 1 Pages: 1-15
- DOI
  10.1186/s13059-018-1400-x
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16K15063, KAKENHI-PROJECT-16H05049, KAKENHI-PROJECT-15K14371, KAKENHI-PROJECT-16K18821, KAKENHI-PROJECT-16K07087
[Journal Article] Efficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System.2017
- Author(s)
  Sato M, Miyoshi K, Nakamura S, Ohtsuka M, Sakurai T, Watanabe S, Kawaguchi H, Tanimoto A.
- Journal Title
  
  Int J Mol Sci
  
  Volume: 18 Issue: 12 Pages: 2610-2610
- DOI
  10.3390/ijms18122610
- NAID
  120007099791
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-16K08023, KAKENHI-PROJECT-16K08085, KAKENHI-PROJECT-16K11810, KAKENHI-PROJECT-16K15063, KAKENHI-PROJECT-16H05049, KAKENHI-PROJECT-16H05176, KAKENHI-PROJECT-15K07695, KAKENHI-PROJECT-16K15233
[Journal Article] Bone marrow-derived mesenchymal stem cells propagate immunosuppressive/anti-inflammatory macrophages in cell-to-cell contact-independent and -dependent manners under hypoxic culture2017
- Author(s)
  Takizawa N, Okubo N, Kamo M, Chosa N, Mikami T, Suzuki K, Yokota S, Ibi M, Ohtsuka M, Taira M, Sasaki D, Yaegashi T, Ishisaki A, Kyakumoto S
- Journal Title
  
  Experimental Cell Res.
  
  Volume: 358 Issue: 2 Pages: 411-420
- DOI
  10.1016/j.yexcr.2017.07.014
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16K11630, KAKENHI-PROJECT-16K11654, KAKENHI-PROJECT-17K11851, KAKENHI-PROJECT-17K11917, KAKENHI-PROJECT-16H04685, KAKENHI-PROJECT-15K11234
[Journal Article] Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins2017
- Author(s)
  Quadros RM, Miura H, Harms DW, Akatsuka H, Sato T, Aida T, Redder R, Richardson GP, Inagaki Y, Sakai D, Buckley SM, Seshacharyulu P, Batra SK, Behlke MA, Zeiner SA, Jacobi AM, Izu Y, Thoreson WB, Urness LD, Mansour SL, Ohtsuka M, Gurumurthy CB
- Journal Title
  
  Genome Biology
  
  Volume: 18 Issue: 1 Pages: 92-92
- DOI
  10.1186/s13059-017-1220-4
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16K07085, KAKENHI-PROJECT-16H04685, KAKENHI-PROJECT-16K18821, KAKENHI-PROJECT-17H04166
[Journal Article] Nucleic acids delivery methods for genome editing in zygotes and embryos: The old, the new, and the old-new.2016
- Author(s)
  Sato M, Ohtsuka M, Watanabe W, Gurumurthy CB
- Journal Title
  
  Biology Direct
  
  Volume: 11 Issue: 1 Pages: 16-16
- DOI
  10.1186/s13062-016-0115-8
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K07695, KAKENHI-PROJECT-15K14371
[Journal Article] Characterization of kidney and skeleton phenotypes of mice double-heterozygous for Foxc1 and Foxc22016
- Author(s)
  Masaru Motojima, Sho Tanimoto, Masato Otsuka, Taiji Matsusaka, Tsutomu Kume , Koichiro Abe
- Journal Title
  
  Cells Tissues Organs
  
  Volume: In Press
- Peer Reviewed / Acknowledgement Compliant / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-25461234
[Journal Article] The piggyBac-Based Gene Delivery System Can Confer Successful Production of Cloned Porcine Blastocysts with Multigene Constructs.2016
- Author(s)
  Sato M, Maeda K, Koriyama M, Inada E, Saitoh I, Miura H, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Miyoshi K.
- Journal Title
  
  Int J Mol Sci.
  
  Volume: 17 Issue: 9 Pages: 1424-1424
- DOI
  10.3390/ijms17091424
- NAID
  130007023583
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-16H05049, KAKENHI-PROJECT-25293418, KAKENHI-PROJECT-25463192, KAKENHI-PROJECT-15K07695, KAKENHI-PROJECT-16K08085, KAKENHI-PROJECT-16K11810, KAKENHI-PROJECT-15K14371, KAKENHI-PROJECT-16K15063
[Journal Article] Characterization of Kidney and Skeleton Phenotypes of Mice Double Heterozygous for Foxc1 and Foxc22016
- Author(s)
  Motojima M, Tanimoto S, Ohtsuka M, Matsusaka T, Kume T, Abe K
- Journal Title
  
  Cells Tissues Organs
  
  Volume: 201 Issue: 5 Pages: 380-389
- DOI
  10.1159/000445027
- Peer Reviewed / Acknowledgement Compliant / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-25461234
[Journal Article] GONAD: a novel CRISPR/Cas9 genome editing method that does not require ex vivo handling of embryos2016
- Author(s)
  Gurumurthy C.B., Takahashi G., Wada K., Miura H., Sato M., and Ohtsuka M.
- Journal Title
  
  Curr. Protoc. Hum. Genet.
  
  Volume: 88 Issue: 1
- DOI
  10.1002/0471142905.hg1508s88
- Peer Reviewed / Acknowledgement Compliant / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K14371, KAKENHI-PROJECT-15K07695
[Journal Article] Pronuclear Injection-based Targeted Transgenesis (PITT)2016
- Author(s)
  Schilit S.L.P., Ohtsuka M., Quadros R. and Gurumurthy C.B.
- Journal Title
  
  Curr. Protoc. Hum. Genet.
  
  Volume: 91 Issue: 1
- DOI
  10.1002/cphg.23
- Peer Reviewed / Acknowledgement Compliant / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16H04685
[Journal Article] Conditional knockout of Foxc2 gene in kidney: efficient generation of conditional alleles of single-exon gene by double-selection system2016
- Author(s)
  Motojima, Masaru; Ogiwara, Sanae; Matsusaka, Taiji; Kim, Sang Yong; Sagawa, Nobuho; Abe, Koichiro; Ohtsuka, Masato
- Journal Title
  
  Mamm Genome
  
  Volume: 27 Issue: 1-2 Pages: 62-69
- DOI
  10.1007/s00335-015-9610-y
- Peer Reviewed / Acknowledgement Compliant / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-25461234, KAKENHI-PROJECT-23249047, KAKENHI-PROJECT-25290035
[Journal Article] PiggyBac transposon-mediated gene delivery efficiently generates stable transfectants derived from cultured primary human deciduous tooth dental pulp cells (HDDPCs) and HDDPC-derived iPS cells.2015
- Author(s)
  Inada E, Saito I, Watanabe S, Aoki R, Miura H, Ohtsuka M, Murakami T, Sawami T, Yamasaki Y, Sato M
- Journal Title
  
  International Journal of Oral Science
  
  Volume: 7 Issue: 3 Pages: 144-145
- DOI
  10.1038/ijos.2015.18
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-15K07695, KAKENHI-PROJECT-25463192, KAKENHI-PROJECT-15K14371, KAKENHI-PROJECT-15J03924, KAKENHI-PROJECT-25293418
[Journal Article] Insertion of sequences at the original provirus integration site of mouse ROSA26 locus using the CRISPR/Cas9 system2015
- Author(s)
  Quadros R., Harms D., Ohtsuka M. and Gurumurthy C. B.
- Journal Title
  
  FEBS Open Bio
  
  Volume: 5 Issue: 1 Pages: 191-197
- DOI
  10.1016/j.fob.2015.03.003
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-25290035
[Journal Article] Assessment of artificial miRNA architectures for higher knockdown efficiencies without the undesired effects in mice2015
- Author(s)
  Miura H., Inoko H., Tanaka M., Nakaoka H., Kimura M., Gurumurthy C. B., Sato M. and Ohtsuka M.
- Journal Title
  
  PLoS ONE
  
  Volume: 10 Issue: 8 Pages: e0135919-e0135919
- DOI
  10.1371/journal.pone.0135919
- Peer Reviewed / Acknowledgement Compliant / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-25290035
[Journal Article] Establishment of immortalized mesenchymal stem cells derived from the submandibular glands of tdTomato transgenic mice2015
- Author(s)
  Furukawa S., Kuwajima Y., Chosa N., Satoh K., Ohtsuka M., Miura H., Kimura M., Inoko H., Ishisaki A., Fujimura A. and Miura H.
- Journal Title
  
  Exp. Ther. Med.
  
  Volume: 10 Pages: 1380-1386
- NAID
  120006334507
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-25290035
[Journal Article] One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT)2015
- Author(s)
  Ohtsuka M., Miura H., Mochida K., Hirose M., Hasegawa A., Ogura A., Mizutani R., Kimura M., Isotani A., Ikawa M., Sato M. and Gurumurthy C. B.
- Journal Title
  
  BMC Genomics
  
  Volume: 16 Issue: 1 Pages: 274-274
- DOI
  10.1186/s12864-015-1432-5
- NAID
  120006957412
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-24700435, KAKENHI-PROJECT-15K07695, KAKENHI-INTERNATIONAL-15K21737, KAKENHI-ORGANIZER-25112001, KAKENHI-PROJECT-25250014, KAKENHI-PROJECT-25290035
[Journal Article] GONAD: Genome-editing via Oviductal Nucleic Acids Delivery system: a novel microinjection independent genome engineering method in mice.2015
- Author(s)
  Takahashi G, Gurumurthy CB, Wada K, Miura H, Sato M, Ohtsuka M
- Journal Title
  
  Scientific Reports
  
  Volume: 5 Issue: 1 Pages: 11406-11406
- DOI
  10.1038/srep11406
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K07695, KAKENHI-PROJECT-15K14371
[Journal Article] トランスジェニックマウスの基礎と応用2014
- Author(s)
  大塚正人、佐藤正宏
- Journal Title
  
  小児外科
  
  Volume: 46 Pages: 571-574
- Data Source
  KAKENHI-PROJECT-25290035
[Journal Article] Neuropathy Target Esterase (神経障害標的エステラーゼ）遺伝子導入マウスの作製2014
- Author(s)
  加賀谷徹、割田貴之、三浦浩美、大塚正人、大久保朋一、坂部貢、木村穰
- Journal Title
  
  臨床環境医学
  
  Volume: 23(1)
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-24651064
[Journal Article] Mouse Genome Editing Using the CRISPR/Cas System2014
- Author(s)
  Harms DW.*, Quadros RM., Seruggia D., Ohtsuka M., Takahashi G., Montoliu L., and Gurumurthy CB
- Journal Title
  
  Curr Protoc Hum Genet
  
  Volume: 83 Issue: 1
- DOI
  10.1002/0471142905.hg1507s83
- Peer Reviewed / Acknowledgement Compliant
- Data Source
  KAKENHI-PROJECT-25290035
[Journal Article] piggyBac-mediated generation of stable transfectants with surface human leukocyte antigen expression from a small number of cells2013
- Author(s)
  Miura H, Inoko H, Inoue I, Okada Y, Tanaka M, Sato M, Ohtsuka M
- Journal Title
  
  Anal Biochem
  
  Volume: 437(1) Pages: 29-31
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23791997
[Journal Article] PiggyBac-mediated generation of stable transfectants with surface HLA expression from a small number of cells.2013
- Author(s)
  Miura H., Inoko H., Inoue I., Okada Y., Tanaka M., Sato M. and Ohtsuka M.
- Journal Title
  
  Anal. Biochem
  
  Volume: 437 Issue: 1 Pages: 29-31
- DOI
  10.1016/j.ab.2013.02.003
- Peer Reviewed
- Data Source
  KAKENHI-PLANNED-22133002, KAKENHI-PROJECT-23592892, KAKENHI-PROJECT-23593038, KAKENHI-PROJECT-23700514, KAKENHI-PROJECT-23700562, KAKENHI-PROJECT-24580411, KAKENHI-PROJECT-24700435, KAKENHI-PROJECT-25290035
[Journal Article] Improvement of pronuclear injection-based targeted transgenesis (PITT) by iCre mRNA-mediated site-specific recombination2013
- Author(s)
  Ohtsuka M, Miura H, Hayashi H, Nakaoka H, Kimura M, Sato M, Gurumurthy CB, Inoko H
- Journal Title
  
  Transgenic Research
  
  Volume: 22 Issue: 4 Pages: 873-875
- DOI
  10.1007/s11248-013-9703-x
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23592892, KAKENHI-PROJECT-23593038, KAKENHI-PROJECT-23700514, KAKENHI-PROJECT-23700562, KAKENHI-PROJECT-24700435, KAKENHI-PROJECT-25290035, KAKENHI-PROJECT-24651064
[Journal Article] Targeted Toxin-Based Selectable Drug-Free Enrichment of Mammalian Cells with High Transgene Expression2013
- Author(s)
  Sato M, Akasaka E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S
- Journal Title
  
  Biology
  
  Volume: 2 Issue: 1 Pages: 341-355
- DOI
  10.3390/biology2010341
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22780274, KAKENHI-PROJECT-23592892, KAKENHI-PROJECT-23593038, KAKENHI-PROJECT-23700514, KAKENHI-PROJECT-23700562, KAKENHI-PROJECT-24580411, KAKENHI-PLANNED-22133002
[Journal Article] X-ray microtomographic visualization of Escherichia coli by metalloprotein overexpression2013
- Author(s)
  Mizutani, R., Taguchi, K., Ohtsuka, M., Kimura, M., Takeuchi, A., Uesugi, K. and Suzuki, Y
- Journal Title
  
  Journal of Synchrotron Radiation
  
  Volume: 20 Pages: 581-586
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-24651064
[Journal Article] Improvement of pronuclear injection-based targeted transgenesis (PITT) by iCre mRNA-mediated site-specific recombination2013
- Author(s)
  Ohtsuka, M., Miura, H., Hayashi, H., Nakaoka, H., Kimura, M.., Sato, M. and Gurumurthy , CB
- Journal Title
  
  Transgenic Research
  
  Volume: 111 Pages: 111-222
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-24651064
[Journal Article] cells consist of two cell types, as evidenced by cytochemical staining for alkaline phosphatase activity: A possible model for cancer stem cell study2013
- Author(s)
  Sato M., Kubota N, Inada E, Saitoh I., Ohtsuka M., Nakamura S., Sakurai T., Watanabe S. HeLa
- Journal Title
  
  Advances in Stem Cells
  
  Pages: 208514-208514
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23700514
[Journal Article] Site-targeted non-viral gene delivery by direct DNA injection into the pancreatic parenchyma and subsequent in vivo electroporation in mice.2013
- Author(s)
  Sato M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T and Watanabe S
- Journal Title
  
  Biotechnology Journal
  
  Volume: 8 Issue: 11 Pages: 1355-1361
- DOI
  10.1002/biot.201300169
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23592892, KAKENHI-PROJECT-23593038, KAKENHI-PROJECT-23700562, KAKENHI-PROJECT-24501317, KAKENHI-PROJECT-24580411, KAKENHI-PROJECT-25290035, KAKENHI-PROJECT-25463192
[Journal Article] Functional Recovery of a Whole Ovary Transplanted Into Syngenic Testis in Mice2012
- Author(s)
  Sato M, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Yasuoka Y
- Journal Title
  
  Clon Transgen
  
  Volume: 1 Issue: 01 Pages: 102-102
- DOI
  10.4172/2168-9849.1000102
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22780274, KAKENHI-PROJECT-23700514, KAKENHI-PROJECT-24580411, KAKENHI-PROJECT-24790222, KAKENHI-PLANNED-22133002
[Journal Article] PITT: pronuclear injection-based targeted transgenesis, a reliable transgene expression method in mice2012
- Author(s)
  Ohtsuka M, Miura H, Sato M, Kimura M, Inoko H, Gurumurthy CB
- Journal Title
  
  Exp Anim
  
  Volume: 61(5) Pages: 489-502
- NAID
  10031121853
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23791997
[Journal Article] In vivo gene transfer in mouse preimplantation embryos after intraoviductal injection of plasmid DNA and subsequent in vivo electroporation2012
- Author(s)
  Sato M
- Journal Title
  
  Syst Biol Reprod Med
  
  Volume: 58 Issue: 5 Pages: 278-287
- DOI
  10.3109/19396368.2012.688088
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22592050, KAKENHI-PROJECT-23592892, KAKENHI-PROJECT-23593038, KAKENHI-PROJECT-23700514, KAKENHI-PROJECT-23700562, KAKENHI-PROJECT-23792439, KAKENHI-PROJECT-24580411, KAKENHI-PLANNED-22133002
[Journal Article] A simplified protocol for the semi-large scale recovery of plasmids from Escherichia coli grown on agar plates2012
- Author(s)
  Sato M, Akasaka E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S
- Journal Title
  
  J Biomed Sci Eng.
  
  Volume: 5 Issue: 07 Pages: 406-408
- DOI
  10.4236/jbise.2012.57051
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22780274, KAKENHI-PROJECT-23592892, KAKENHI-PROJECT-23593038, KAKENHI-PROJECT-23700514, KAKENHI-PROJECT-23700562, KAKENHI-PROJECT-23792439, KAKENHI-PROJECT-24580411, KAKENHI-PLANNED-22133002
[Journal Article] PITT: Pronuclear Injection-Based Targeted Transgenesis, a Reliable Transgene Expression Method in Mice2012
- Author(s)
  Ohtsuka, M., Miura, H., Sato, M., Kimura, M., Inoko H., Gurumurthy, CB.
- Journal Title
  
  Exp Anim.
  
  Volume: 61 Pages: 489-502
- NAID
  10031121853
- Data Source
  KAKENHI-PROJECT-24651064
[Journal Article] Development of a technique for efficient gene transfer to antral follicular cells in the mouse ovary2012
- Author(s)
  Sato M, Akasaka E, Saitoh I, Ohtsuka M, Watanabe S
- Journal Title
  
  Syst Biol Reprod Med
  
  Volume: 58(3) Pages: 136-41
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23791997
[Journal Article] Targeted transgenesis through pronuclear injection of improved vectors into in vitro fertilized eggs2012
- Author(s)
  Ohtsuka M, Miura H, Nakaoka H, Kimura M, Sato M, Inoko H
- Journal Title
  
  Transgenic Res
  
  Volume: 21(1) Pages: 225-6
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23791997
[Journal Article] Development of a technique for efficient gene transfer to antral follicular cells in the mouse ovary2012
- Author(s)
  Sato M, Akasaka E, Saitoh I, Ohtsuka M, Watanabe S
- Journal Title
  
  Systems Biology in Reproductive Medicine
  
  Volume: 58 Issue: 3 Pages: 136-141
- DOI
  10.3109/19396368.2012.656796
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23592892, KAKENHI-PROJECT-23593038, KAKENHI-PROJECT-23700514, KAKENHI-PROJECT-23700562, KAKENHI-PROJECT-23792439, KAKENHI-PROJECT-24580411, KAKENHI-PLANNED-22133002
[Journal Article] 赤色蛍光強発現遺伝子導入マウスにおける蛍光発現部位の形態学的検討2012
- Author(s)
  桑島幸紀、大塚正人、石崎明、藤村朗
- Journal Title
  
  岩手医科大学歯学雑誌
  
  Volume: 37 Pages: 24-37
- NAID
  110009445046
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23592896
[Journal Article] Production of miRNA-based knockdown mice showing reproducible knockdown of the gene of interest2012
- Author(s)
  Miura H., Inoko H., Kimura M. and Ohtsuka M.
- Journal Title
  
  J. Reprod. Develop.
  
  Volume: 58
- NAID
  130007024276
- Data Source
  KAKENHI-PROJECT-23700514
[Journal Article] Fluorescent transgenic mice suitable for multi-color aggregation chimera studies2012
- Author(s)
  Ohtsuka M, Miura H, Gurumurthy CB, Kimura M, Inoko H, Yoshimura S, Sato M
- Journal Title
  
  Cell and Tissue Research
  
  Volume: 350 Issue: 2 Pages: 251-260
- DOI
  10.1007/s00441-012-1470-0
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23592892, KAKENHI-PROJECT-23593038, KAKENHI-PROJECT-23700514, KAKENHI-PROJECT-23700562, KAKENHI-PROJECT-24580411, KAKENHI-PROJECT-24700435, KAKENHI-PROJECT-24651064, KAKENHI-PLANNED-22133002
[Journal Article] Determination of the Optimal Concentration of Several Selective Drugs Useful for Generating Multi-Transgenic Porcine Embryonic Fibroblasts2012
- Author(s)
  Sato M, Ohtsuka M, Miura H, Miyoshi K, Watanabe S.
- Journal Title
  
  Reproduction in Domestic Animals/John Wiley & Sons, Inc.
  
  Volume: 47 Issue: 5 Pages: 759-765
- DOI
  10.1111/j.1439-0531.2011.01964.x
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350, KAKENHI-PROJECT-22580321, KAKENHI-PROJECT-23592892, KAKENHI-PROJECT-23593038, KAKENHI-PROJECT-23700514, KAKENHI-PROJECT-23700562, KAKENHI-PROJECT-24580411, KAKENHI-PROJECT-24700435, KAKENHI-PLANNED-22133002
[Journal Article] PITT: Pronuclear Injection-Based Targeted Transgenesis, a Reliable Transgene Expression Method in Mice2012
- Author(s)
  Ohtsuka M, Miura H, Sato M, Kimura M, Inoko H, Gurumurthy CB
- Journal Title
  
  Exp. Anim.
  
  Volume: 61 Issue: 5 Pages: 489-502
- DOI
  10.1538/expanim.61.489
- NAID
  10031121853
- ISSN
  0007-5124, 1341-1357, 1881-7122
- Language
  English
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23592892, KAKENHI-PROJECT-23593038, KAKENHI-PROJECT-23700514, KAKENHI-PROJECT-23700562, KAKENHI-PROJECT-24700435, KAKENHI-PLANNED-22133002
[Journal Article] Targeted transgenesis through pronuclear injection of improved vectors into in vitro fertilized eggs2011
- Author(s)
  Ohtsuka M, Miura H, Nakaoka H, Kimura M, Sato M, Inoko H
- Journal Title
  
  Transgenic Res
  
  Volume: 21 Pages: 225-226
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19101008
[Journal Article] Targeted transgenesis through pronuclear injection of improved vectors into in vitro fertilized eggs2011
- Author(s)
  Ohtsuka, M., Miura, H., Nakaoka, H., Kimura, M., Sato, M., Inoko, H.
- Journal Title
  
  Transgenic Research
  
  Volume: 21:1 Issue: 1 Pages: 225-226
- DOI
  10.1007/s11248-011-9505-y
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19101008, KAKENHI-PROJECT-23390191, KAKENHI-PROJECT-23592892, KAKENHI-PROJECT-23593038, KAKENHI-PROJECT-23700514, KAKENHI-PROJECT-23700562, KAKENHI-PROJECT-24580411
[Journal Article] PITT法――マウス受精卵での部位特異的遺伝子導入法2011
- Author(s)
  大塚正人
- Journal Title
  
  実験医学
  
  Volume: 29 Pages: 2687-2692
- Data Source
  KAKENHI-PROJECT-23700514
[Journal Article] Simple cloning strategy using GFPuv gene as positive/negative indicator2011
- Author(s)
  Miura Hiromi; Inoko Hidetoshi; Inoue Ituro, et al
- Journal Title
  
  ANALYTICAL BIOCHEMISTRY
  
  Volume: 416:2 Issue: 2 Pages: 237-239
- DOI
  10.1016/j.ab.2011.04.040
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19101008, KAKENHI-PROJECT-23390191, KAKENHI-PROJECT-23700514
[Journal Article] Loss of glial fibrillary acidic protein marginally accelerates disease progression in a SOD1(H46R) transgenic mouse model of ALS2011
- Author(s)
  Yasuhiro Yoshii
- Journal Title
  
  Neuroscience Research
  
  Volume: 30巻 Issue: 3 Pages: 321-329
- DOI
  10.1016/j.neures.2011.03.006
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21700397, KAKENHI-PROJECT-23300129, KAKENHI-PROJECT-23700514
[Journal Article] Targeted transgenesis through pronuclear injection of improved vectors into in vitro fertilized eggs.2011
- Author(s)
  Ohtsuka M, Miura H, Nakaoka H, Kimura M, Sato M, Inoko H.
- Journal Title
  
  Transgenic Res
  
  Volume: Mar 25 Pages: 1-2
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19101008
[Journal Article] Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression.2010
- Author(s)
  Ohtsuka M, Ogiwara S, Miura H, Mizutani A, Warita T, Sato M, Imai K, Hozumi K, Sato T, Tanaka M, Kimura M, Inoko H
- Journal Title
  
  Nucleic Acids Res
  
  Volume: 38
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19101008
[Journal Article] Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression.2010
- Author(s)
  Ohtsuka M., Ogiwara S., Miura H., Mizutani A., Warita T., Sato M., Imai K., Hozumi K., Sato T., Tanaka M., Kimura M., Inoko H.
- Journal Title
  
  Nucleic Acids Res. 38
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20700368
[Journal Article] Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression2010
- Author(s)
  Ohtsuka M, Ogiwara S, Miura H, Mizutani A, Warita T, Sato M, Imai K, Hozumi K, Sato T, Tanaka M, Kimura M, Inoko H
- Journal Title
  
  Nucleic Acids Res
  
  Volume: 38
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19101008
[Journal Article] Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression.2010
- Author(s)
  Ohtsuka M., et al.
- Journal Title
  
  Nucleic Acids Res.
  
  Volume: 38
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20700368
[Journal Article] Recombinant DNA technologies for construction of precisely designed transgene constructs2009
- Author(s)
  Ohtsuka M, Kimura M, Tanaka M, Inoko H
- Journal Title
  
  Curr Pharm Biotechnol
  
  Volume: 10 Pages: 244-251
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19101008
[Journal Article] Recombinant DNA technologies for construction of precisely designed transgene constructs2009
- Author(s)
  Ohtsuka M., et.al.
- Journal Title
  
  Curr. Pharm. Biotechnol. 10
  
  Pages: 244-251
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20700368
[Journal Article] Development of CRTEIL and CETRIZ, Cre-loxP-based systems, which allow change of expression of red to green or green to red fluorescence upon transfection with a Cre expression vector2009
- Author(s)
  Ohtsuka M., et al.
- Journal Title
  
  J Biomed Biotechnol.
  
  Pages: 985140-985140
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20700368
[Journal Article] A transposon-based chromosomal engineering method to survey a large cis-regulatory landscape in the mouse genome.2009
- Author(s)
  Kokubu C., Horie K., Abe K., Ikeda R., Mizuno S., Uno Y., Ogiwara S., Ohtsuka M., Isotani A., Okabe M., Imai K., Takeda J.
- Journal Title
  
  Nat.Genet. 41
  
  Pages: 946-952
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20700368
[Journal Article] Recombinant DNA technologies for construction of precisely designed transgene constructs.2009
- Author(s)
  Ohtsuka M, Kimura M, Tanaka M, Inoko H
- Journal Title
  
  Curr Pharm Biotechnol 10
  
  Pages: 244-251
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19101008
[Journal Article] Development of CRTEIL and CETRIZ, Cre-loxP-based systems, which allow change of expression of red to green or green to red fluorescence upon transfection with a Cre expression vector2009
- Author(s)
  Ohtsuka M., et.al.
- Journal Title
  
  J Biomed Biotechnol (in press)
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20700368
[Journal Article] Development of CRTEIL and CETRIZ, Cre-loxP-based systems, which allow change of expression of red to green or green to red fluorescence upon transfection with a Cre expression vector.2009
- Author(s)
  Ohtsuka M., Warita T., Sakurai T., Watanabe S., Inoko H., Sato M.
- Journal Title
  
  J Biomed Biotechnol. 985140
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20700368
[Journal Article] Recombinant DNA technologies for construction of precisely designed transgene constructs.2009
- Author(s)
  Ohtsuka M., Kimura, M., Tanaka, M., Inoko, H.
- Journal Title
  
  Curr.Pharm.Biotechnol. 10
  
  Pages: 244-251
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20700368
[Journal Article] Cre-loxP System As a Versatile Tool for Conferring Increased Levels of Tissue-Specific Gene Expression from a Weak Promoter.2008
- Author(s)
  Nakamura S., Watanabe S., Ohtsuka M., Maehara T., Ishihara M., Yokomine T., Sato M.
- Journal Title
  
  Mol.Reprod.Dev. 75
  
  Pages: 1085-1093
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20700368
[Journal Article] Major histocompatibility complex (Mhc) class lb gene duplications, organization and expression patterns in mouse strain C57BL/6.2008
- Author(s)
  Ohtsuka M. lnoko H. Kulski J K, Yoshimura S
- Journal Title
  
  BMC Genomics 9
  
  Pages: 178-192
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19101008
[Journal Article] Major histocompatibility complex(Mhc)class Ib gene duplications, organization and expression patterns in mouse strain C57BL/62008
- Author(s)
  Ohtsuka M., et.al.
- Journal Title
  
  BMC Genomics 9
  
  Pages: 178-178
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20700368
[Journal Article] One-step generation of recombineering constructs by asymmetric-end ligation and negative selection2007
- Author(s)
  Ohtsuka M, Mizutani A, Kikuti YY, Knlski JK, Sato M, Kimura M, Tanaka M, Inoko H
- Journal Title
  
  Anal Biochem 360
  
  Pages: 306-308
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19101008
[Journal Article] One-step generation of recombineering constructs by asymmetric-end ligation and negative selection2007
- Author(s)
  Ohtsuka M, Mizutani A, Kikuti YY, Kulski JK, Sato M, Kimura M, Tanaka M, Inoko H
- Journal Title
  
  Anal Biochem
  
  Volume: 360 Pages: 306-308
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19101008
[Journal Article] An EYFP insertion mutant containing a modified lox sequence for potential use as a recombination indicator2005
- Author(s)
  Mizutani A., Ohtsuka M.et al.
- Journal Title
  
  Nucleic Acids Symp Ser. 49
  
  Pages: 297-298
- Data Source
  KAKENHI-PROJECT-16651102
[Journal Article] Usefulness of repeated GenomiPhi, a phi29 DNA polymerase-based rolling circle amplification kit, for generation of large amounts of plasmid DNA.2005
- Author(s)
  Sato M., Ohtsuka M., Ohmi Y.
- Journal Title
  
  Biomol Eng. 22
  
  Pages: 129-132
- Data Source
  KAKENHI-PROJECT-16651102
[Journal Article] Possible roles of zic1 and zic4, identified within the medaka Double anal fin(Da)locus, in dorsoventral patterning of the trunk-tail region(related to the phenotypes of the Da mutant).2004
- Author(s)
  Otsuka, M., et al.
- Journal Title
  
  Mechanism of Development 121
  
  Pages: 873-882
- Data Source
  KAKENHI-PROJECT-16659091
[Journal Article] CHOP : visualization of ‘wobbling' and isolation of highly conserved regions from aligned DNA sequences.2004
- Author(s)
  Otsuka, M., et al.
- Journal Title
  
  Nucleic Acid Research 32
- Data Source
  KAKENHI-PROJECT-16659091
[Journal Article] CHOP : visualization of 'wobbling' and isolation of highly conserved regions from aligned DNA sequences2004
- Author(s)
  Ohtsuka M. et al.
- Journal Title
  
  Nucleic Acids Res. 32
- Data Source
  KAKENHI-PROJECT-16651102
[Journal Article] Direct subcloning of target region from BAC insert using restriction enzymes that produce non-identical cohesive ends2004
- Author(s)
  Ohmi Y., Sato M., Ohtsuka M
- Journal Title
  
  Nucleic Acids Symp Ser. 48
  
  Pages: 141-142
- Data Source
  KAKENHI-PROJECT-16651102
[Journal Article] Construction of mouse 129/Ola BAC library for the targeting experiments using E14 embryonic stem cells.
- Author(s)
  Ohtsuka M. et al.
- Journal Title
  
  Genes Genet Syst. (in press)
- NAID
  10019162105
- Data Source
  KAKENHI-PROJECT-16651102
[Journal Article] Targeted transgenesis through pronuclear injection of improved vectors into in vitro fertilized eggs.
- Author(s)
  Ohtsuka M., et al.
- Journal Title
  
  Transgenic Res.
  
  Volume: (in press)
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20700368
[Journal Article] Targeted transgenesis through pronuclear injection of improved vectors into in vitro fertilized eggs.
- Author(s)
  Ohtsuka M., Miura H., Nakaoka H., Kimura M., Sato M., Inoko H.
- Journal Title
  
  Transgenic Res. in press
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20700368
[Patent] METHODS AND COMPOSITIONS FOR IN SITU GERMLINE GENOME ENGINEERING2018
- Inventor(s)
  OHTSUKA Masato 他2名
- Industrial Property Rights Holder
  OHTSUKA Masato 他2名
- Industrial Property Rights Type
  特許
- Filing Date
  2018
- Acquisition Date
  2018
- Data Source
  KAKENHI-PROJECT-18K11128
[Patent] 哺乳動物の受精卵に物質を導入する方法2017
- Inventor(s)
  大塚　正人、森泉　俊幸、森泉　康裕
- Industrial Property Rights Holder
  株式会社ベックス、学校法人東海大学
- Industrial Property Rights Type
  特許
- Filing Date
  2017
- Data Source
  KAKENHI-PROJECT-15K14371
[Patent] Dna editing using single-stranded dna2016
- Inventor(s)
  C B GURU, H Miura, M Ohtsuka
- Industrial Property Rights Holder
  The Univ. Nebraska, Tokai Univ.
- Industrial Property Rights Type
  特許
- Filing Date
  2016-06-03
- Overseas
- Data Source
  KAKENHI-PROJECT-16H04685
[Patent] 部位特異的組換え系を利用した遺伝子導入方法およびそのためのキット2012
- Inventor(s)
  大塚正人
- Industrial Property Rights Holder
  東海大学
- Filing Date
  2012-11-28
- Data Source
  KAKENHI-PROJECT-23700514
[Patent] 標的遺伝子のノックダウン方法およびそのためのＲＮＡｉ用発現ベクター2012
- Inventor(s)
  大塚正人、三浦浩美
- Industrial Property Rights Holder
  東海大学
- Industrial Property Rights Type
  特許
- Industrial Property Number
  2012-259950
- Filing Date
  2012-11-28
- Data Source
  KAKENHI-PLANNED-22133002
[Patent] 部位特異的組換え系を利用した遺伝子導入方法およびそのためのキット2012
- Inventor(s)
  大塚正人
- Industrial Property Rights Holder
  東海大学
- Industrial Property Rights Type
  特許
- Industrial Property Number
  2012-259951
- Filing Date
  2012-11-28
- Data Source
  KAKENHI-PLANNED-22133002
[Patent] 標的遺伝子のノックダウン方法およびそのためのＲＮＡｉ用発現ベクター2012
- Inventor(s)
  大塚正人、三浦浩美
- Industrial Property Rights Holder
  東海大学
- Industrial Property Rights Type
  特許
- Industrial Property Number
  2012-259950
- Filing Date
  2012-11-28
- Data Source
  KAKENHI-PROJECT-23700514
[Patent] 部位特異的組換え系を利用した遺伝子導入方法およびそのためのキット2012
- Inventor(s)
  大塚正人
- Industrial Property Rights Holder
  東海大学
- Industrial Property Rights Type
  特許
- Industrial Property Number
  2012-259951
- Filing Date
  2012-11-28
- Data Source
  KAKENHI-PROJECT-23700514
[Patent] 標的遺伝子のノックダウン方法およびそのためのRNAi用発現ベクター2012
- Inventor(s)
  大塚正人、三浦浩美
- Industrial Property Rights Holder
  東海大学
- Filing Date
  2012-11-28
- Data Source
  KAKENHI-PROJECT-23700514
[Patent] リコンビニアリングコンストラクト、及びジーンターゲティングコンストラクト作製用ベクター2005
- Inventor(s)
  大塚正人, 水谷晃子, 田中正史, 猪子英俊
- Industrial Property Rights Holder
  学校法人東海大学
- Industrial Property Number
  2005-318209
- Filing Date
  2005-11-01
- Data Source
  KAKENHI-PROJECT-16651102
[Presentation] i-GONAD: A Method for Generation of Genome Edited Animals without Ex vivo Handling of Embryos2021
- Author(s)
  大塚正人
- Organizer
  第85回日本循環器学会学術集会、ISHR U45企画セッション
- Invited
- Data Source
  KAKENHI-PROJECT-18K11128
[Presentation] 加齢卵巣の低排卵応答に関与する遺伝子ネットワーク2019
- Author(s)
  諌山慧士朗、渡邉健司、村田智昭、大塚正人、水上洋一
- Organizer
  山口大学生命医工学シンポジウム
- Data Source
  KAKENHI-PROJECT-18K11128
[Presentation] Development of a Reporter Mouse Model For In Vivo Evaluation of Genome Editing2019
- Author(s)
  Masato Ohtsuka
- Organizer
  the American Society of Gene & Cell Therapy (ASGCT) 2019
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16KK0189
[Presentation] 次世代シーケンスとGONAD法を用いた卵巣老化における排卵機能低下の原因遺伝子の解明2019
- Author(s)
  諌山慧士朗、渡邉健司、坂口修一、大塚正人、水上洋一
- Organizer
  日本生化学会中国四国支部例会
- Data Source
  KAKENHI-PROJECT-18K11128
[Presentation] in vivoゲノム編集効率評価系モデルマウスの作製と検証2019
- Author(s)
  大塚正人
- Organizer
  第4回日本遺伝子細胞治療学会若手研究会セミナー2019
- Data Source
  KAKENHI-PROJECT-16KK0189
[Presentation] piggyBac系ベクターのハイドロダイナミクス遺伝子導入法はマウス肝臓での導入遺伝子の持続発現と肝細胞でのgene switchingを可能とする2018
- Author(s)
  中村伸吾、渡部聡、石原雅之、安藤尚子、大塚正人、佐藤正宏
- Organizer
  第41回日本分子生物学会年会
- Data Source
  KAKENHI-PROJECT-16H04685
[Presentation] i-GONAD: A Method for Genome Editing Rodents without ex vivo Handling of Embryos2018
- Author(s)
  Ohtsuka M
- Organizer
  New Frontiers in CRISPR-based Gene Editing (Part of the 25th International Molecular Medicine Tri-Conference)
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] 卵管内注入によるゲノム編集動物作製法(GONAD)の改良:性腺刺激ホルモン投与による実験時間の制御2018
- Author(s)
  佐藤正宏, 大塚正人, 中村伸吾
- Organizer
  第111回日本繁殖生物学会大会
- Data Source
  KAKENHI-PROJECT-16H05049
[Presentation] ゲノム編集効率評価系モデルマウスの開発と評価2018
- Author(s)
  三浦浩美、佐藤正宏、水谷晃子、大塚正人
- Organizer
  第41回日本分子生物学会年会
- Data Source
  KAKENHI-PROJECT-16H04685
[Presentation] ex vivo胚操作が不要なゲノム編集動物作製法「GONAD」の開発とラットへの応用を目指して2018
- Author(s)
  大塚正人
- Organizer
  第11回ラットリソースリサーチ研究会
- Invited
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] Development of a reporter mouse model suitable for evaluation of in vivo genome editing efficiency2018
- Author(s)
  H Miura, CB Gurumurthy, M Ohtsuka
- Organizer
  2018 Cold Spring Harbor meeting: Genome Engineering: The CRISPR/Cas Revolution
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16H04685
[Presentation] 加齢に伴って低下する排卵機能に関与する遺伝子の解明-GONAD法によるゲノム編集マウスの作製-2018
- Author(s)
  水上洋一、諌山慧士朗、渡邉健司、村田智昭、大塚正人
- Organizer
  日本生化学会中四国支部会
- Data Source
  KAKENHI-PROJECT-18K11128
[Presentation] 新規遺伝子改変マウス作製方法：PITT, Easi-CRISPR, GONAD2017
- Author(s)
  大塚正人
- Organizer
  第３回川島カンファレンス
- Invited
- Data Source
  KAKENHI-PROJECT-16H04685
[Presentation] 新規遺伝子改変マウス作製方法：PITT, Easi-CRISPR, GONAD2017
- Author(s)
  大塚正人
- Organizer
  第３回川島カンファレンス
- Invited
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] Development of a mouse model suitable for in vivo genome editing efficiency studies2017
- Author(s)
  Hiromi Miura, Yutaka Inagaki, Channabasavaiah B Gurumurthy, Masahiro Sato, Masato Ohtsuka
- Organizer
  14th Transgenic Technology Meeting (TT2017)
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16H04685
[Presentation] Improved GONAD (i-GONAD) (II): usefulness of rhodamine-dextran for monitoring the success of the GONAD and of gonadotrophin-based regulation of the timing for the GONAD2017
- Author(s)
  Masahiro Sato, Shingo Nakamura, Channabasavaiah B. Gurumurthy, Satoshi Watanabe, Masato Ohtsuka
- Organizer
  14th Transgenic Technology Meeting (TT2017)
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] 遺伝子改変マウス作製の簡便化を実現する新しい手法: GONAD（Genome-editing via Oviductal Nucleic Acids Delivery）法2017
- Author(s)
  大塚正人
- Organizer
  徳島大学「ゲノム編集事業」キックオフシンポジウム
- Invited
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] Improved GONAD (i-GONAD) (I) as ex vivo manipulation-free genome-editing system allowing efficient knock-out, large deletion, and knock-in2017
- Author(s)
  Masato Ohtsuka, Hiromi Miura, Channabasavaiah B. Gurumurthy, Masahiro Sato
- Organizer
  14th Transgenic Technology Meeting (TT2017)
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] 採卵、顕微注入、胚移植のステップが不要なゲノム編集マウス作製法GONADによるノックインマウス作製2017
- Author(s)
  大塚正人, 三浦浩美, 佐藤正宏
- Organizer
  日本ゲノム編集学会第2回大会
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] GONAD: a simple method to generate genome-edited mice without ex vivo handling of embryos. Mouse genome engineering2017
- Author(s)
  Ohtsuka M
- Organizer
  Mouse genome engineering in EMBO Practical Course
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] 簡便なゲノム編集マウス作製法GONAD法を用いたアルビノマウスのレスキュー実験2017
- Author(s)
  高林秀次、青島拓也、佐藤正宏、大塚正人
- Organizer
  第40回日本分子生物学会年会
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] In vivoゲノム編集効率評価系モデルマウスの開発2017
- Author(s)
  三浦浩美、稲垣豊、佐藤正宏、大塚正人
- Organizer
  第40回日本分子生物学会年会
- Data Source
  KAKENHI-PROJECT-16H04685
[Presentation] Development of a mouse model suitable for in vivo genome editing efficiency studies2017
- Author(s)
  Hiromi Miura, Yutaka Inagaki,Channabasavaiah B Gurumurthy, Masahiro Sato, Masato Ohtsuka
- Organizer
  14th Transgenic Technology Meeting (TT2017)
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] 卵管内受精卵を標的とした生体内ゲノム編集法(GONAD)習得のための2日間プロトコール2017
- Author(s)
  大塚正人,中村伸吾,CB. Gurumurthy,Naomi Arifin,Md Atiqul Islam,三浦浩美,佐藤正宏.
- Organizer
  第51回日本実験動物技術者協会総会
- Data Source
  KAKENHI-PROJECT-16H05049
[Presentation] Rescue of the albino phenotype in mice using GONAD method2017
- Author(s)
  Hiromi Miura, Naomi Arifin, Masahiro Sato, Masato Ohtsuka
- Organizer
  International Pigment Cell Conference 2017(IPCC2017)
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] GONAD: ex vivo胚操作を要しないゲノム編集マウス作製手法2017
- Author(s)
  大塚正人
- Organizer
  第40回日本分子生物学会年会　ランチョンセミナー
- Invited
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] 新たなゲノム編集技術GONAD法を用いた遺伝子改変ラットの作製法2017
- Author(s)
  小林朋絵、難波真澄、古家野孝行、佐藤正宏、大塚正人、松山誠
- Organizer
  第40回日本分子生物学会年会
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] In vivoゲノム編集効率評価系モデルマウスの開発2017
- Author(s)
  三浦浩美、稲垣豊、佐藤正宏、大塚正人
- Organizer
  第40回日本分子生物学会年会
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] CRISPR solution for creating Cre-LoxP genetics resources2017
- Author(s)
  Rolen M Quadros, Hiromi Miura, Masato Ohtsuka, Donald W Harms, Channabasavaiah B Gurumurthy
- Organizer
  Genome Engineering: The CRISPR-Cas Revolution（CSHL Meetings & Courses Program）
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16H04685
[Presentation] GONAD法：採卵、顕微注入、胚移植のステップが不要なゲノム編集マウス作製法2016
- Author(s)
  大塚正人、高橋剛、三浦浩美、和田健太、佐藤正宏
- Organizer
  第39回日本分子生物学会年会
- Place of Presentation
  パシフィコ横浜（神奈川県横浜市）
- Year and Date
  2016-11-30
- Invited
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] GONAD法：ex vivo 胚操作を要しないゲノム編集動物作製法2016
- Author(s)
  大塚正人
- Organizer
  SKIP×幹細胞若手の会　Symposium 2016 ～Integrative Stem Cell Biology for Regenerative Medicine～
- Place of Presentation
  御茶ノ水トライエッジカンファレンス（東京都千代田区）
- Invited
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] Decreased Renal Threshold for Urinary Excretion of Ammonia Underlies Proximal Renal Tubular Acidosis in TASK2 K Channel-Deficient Mice2016
- Author(s)
  Yukiko Yasuoka, Tomomi Oshima, Yuichi Sato, Masato Ohtsuka, Minoru Kimura, Hiroshi Nonoguchi, Katsumasa Kawahara.
- Organizer
  アメリカ腎臓学会
- Place of Presentation
  マコーミックプレイス（アメリカ、シカゴ）
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16K08505
[Presentation] GONAD and Easy (Isi)-CRISPR: novel mouse genome engineering tools2016
- Author(s)
  Masato Ohtsuka
- Organizer
  13th Transgenic Technology Meeting (TT2016)
- Place of Presentation
  Prague, Czech Republic
- Year and Date
  2016-03-20
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] Generation of knockdown mice using PITT and Easi-CRISPR2016
- Author(s)
  Ohtsuka M.
- Organizer
  MicroRNAs/Noncoding & Genome Engineering/Editing and Europe -2016
- Place of Presentation
  Peterhouse University of Cambridge（Cambridge, UK）
- Year and Date
  2016-11-01
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16H04685
[Presentation] 長い一本鎖DNAを用いたノックインマウス作製2016
- Author(s)
  三浦浩美、Channabasavaiah Gurumurthy、稲垣豊、大塚正人
- Organizer
  平成28年度新学術領域研究「学術研究支援基盤形成【先端モデル動物支援プラットフォーム】」若手支援技術講習会
- Place of Presentation
  蓼科グランドホテル滝の湯（長野県茅野市）
- Year and Date
  2016-09-14
- Data Source
  KAKENHI-PROJECT-16H04685
[Presentation] Improvement of GONAD (genome-editing via oviductal nucleic acids delivery), one of the zygote injection-free genome editing systems targeted to preimplantation embryos2016
- Author(s)
  Masahiro Sato, Channabasavaiah B. Gurumurthy, Satoshi Watanabe, Masato Ohtsuka
- Organizer
  13th Transgenic Technology Meeting (TT2016)
- Place of Presentation
  Prague, Czech Republic
- Year and Date
  2016-03-20
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] GONAD and Easy (Isi)-CRISPR: novel mouse genome engineering tools2016
- Author(s)
  Masato Ohtsuka
- Organizer
  13th Transgenic Technology Meeting
- Place of Presentation
  Prague, Czech Republic
- Year and Date
  2016-03-20
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] Easi-CRISPR：高効率なノックインマウス作製法2016
- Author(s)
  三浦浩美、Gurumurthy Channabasavaiah、相田知海、稲垣豊、佐藤正宏、佐藤健人、大塚正人
- Organizer
  第39回日本分子生物学会年会
- Place of Presentation
  パシフィコ横浜（神奈川県横浜市）
- Year and Date
  2016-11-30
- Data Source
  KAKENHI-PROJECT-16H04685
[Presentation] piggyBacベクターシステムとin vivo gene transferを用いた新たな肝細胞株樹立の試み2016
- Author(s)
  渡部聡, 中村伸吾, 桜井敬之, 大塚正人, 佐藤正宏.
- Organizer
  第39回日本分子生物学会年会
- Place of Presentation
  パシフィコ横浜
- Year and Date
  2016-11-30
- Data Source
  KAKENHI-PROJECT-16H05049
[Presentation] piggyBac遺伝子導入系により複数遺伝子が同時導入されたブタ細胞は核移植後のクローン胚の発生を保証する2016
- Author(s)
  佐藤正宏, 前田昂亮, 郡山実優, 稲田絵美, 齋藤一誠, 大塚正人, 中村伸吾, 桜井敬之, 渡部聡, 三好和睦.
- Organizer
  第109回日本繁殖生物学会大会
- Place of Presentation
  麻布大学
- Year and Date
  2016-09-11
- Data Source
  KAKENHI-PROJECT-16H05049
[Presentation] Easi-CRISPR：長鎖一本鎖DNAを用いた高効率ノックイン法2016
- Author(s)
  大塚正人，相田知海，三浦浩美，Channabasavaiah Gurumurthy
- Organizer
  日本ゲノム編集学会第1回大会
- Place of Presentation
  広島国際会議場（広島県広島市）
- Year and Date
  2016-09-06
- Data Source
  KAKENHI-PROJECT-16H04685
[Presentation] ex vivo胚操作が不要なゲノム編集マウス作製法2016
- Author(s)
  大塚正人, 高橋　剛, 和田　健太, 三浦浩美, 佐藤正宏
- Organizer
  平成28年度新学術領域研究「学術研究支援基盤形成【先端モデル動物支援プラットフォーム】」若手支援技術講習会
- Place of Presentation
  蓼科グランドホテル滝の湯（長野県茅野市）
- Year and Date
  2016-09-14
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] 医用ブタおける遺伝子組換え動物作製の実際とその可能性について2016
- Author(s)
  佐藤　正宏、渡部　聡、大塚　正人
- Organizer
  第50回日本実験動物技術者協会総会
- Place of Presentation
  ウェスタ川越（埼玉県川越市）
- Year and Date
  2016-09-29
- Invited
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] 採卵、顕微注入、胚移植を要しないゲノム編集マウス作製法GONADによるノックアウトマウス作製2016
- Author(s)
  大塚正人, 高橋　剛, 和田　健太, 三浦浩美, Channabasavaiah B Gurumurthy, 佐藤正宏
- Organizer
  第63回日本実験動物学会総会
- Place of Presentation
  ミューザ川崎シンフォニーホール（神奈川県川崎市）
- Year and Date
  2016-05-18
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] マウスすい臓内への piggyBac系を介した直接生体内遺伝子導入は外来遺伝子の長期発現を可能とする2016
- Author(s)
  佐藤正宏, 稲田絵美, 齋藤一誠, 三浦浩美, 大塚正人, 中村伸吾, 桜井敬之, 渡部聡.
- Organizer
  第39回日本分子生物学会年会
- Place of Presentation
  パシフィコ横浜
- Year and Date
  2016-11-30
- Data Source
  KAKENHI-PROJECT-16H05049
[Presentation] 人工 miRNA を用いたマウス遺伝子ノックダウンシステムの検証2015
- Author(s)
  三浦浩美、木村穣、佐藤正宏、大塚正人
- Organizer
  第62回日本実験動物学会総会
- Place of Presentation
  京都テルサ　（京都府京都市）
- Year and Date
  2015-05-28
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] GONAD法：卵管を介したマウス2細胞期胚へのin vivo RNA導入による新規ゲノム編集マウス作製法2015
- Author(s)
  大塚正人、佐藤正宏、高橋剛
- Organizer
  GE Life Sciences Day 2015
- Place of Presentation
  パシフィコ横浜会議センター　（神奈川県横浜市）
- Year and Date
  2015-07-24
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] GONAD: a novel CRISPR/Cas9 genome editing method that does not require ex vivo handling of fertilized eggs2015
- Author(s)
  Masato Ohtsuka, Gou Takahashi, Kenta Wada, Hiromi Miura, Channabasavaiah B Gurumurthy, Masahiro Sato
- Organizer
  29th International Mammalian Genome Conference (IMGC)
- Place of Presentation
  Yokohama Port Opening Memorial Hall, (Yokohama, Kanagawa, Japan)
- Year and Date
  2015-11-08
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] マウス受精卵への顕微注入を介したターゲットトランスジェネシス法の改良2015
- Author(s)
  大塚正人、三浦浩美、佐藤正宏、木村穣
- Organizer
  第62回日本実験動物学会総会
- Place of Presentation
  京都テルサ　（京都府京都市）
- Year and Date
  2015-05-28
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] 顕微注入法を介したターゲットトランスジェネシスによるノックダウンマウス作製2015
- Author(s)
  三浦浩美、木村穣、大塚正人
- Organizer
  第 29 回モロシヌス研究会
- Place of Presentation
  神戸　かんぽの宿　有馬　（兵庫県神戸市）
- Year and Date
  2015-07-03
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] 神経障害標的エステラーゼ遺伝子発現マウスの特性と有機リンの影響2015
- Author(s)
  本杉奈美、山村勇貴、三浦浩美、大塚正人、大久保朋一、吉野美千代、田中正史、畑中朋美、坂部貢、木村穣
- Organizer
  第62回日本実験動物学会総会
- Place of Presentation
  京都テルサ　（京都府京都市）
- Year and Date
  2015-05-28
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] 卵管を介したマウス 2 細胞期胚への RNA 導入法の開発とゲノム編集技術への応用2015
- Author(s)
  高橋剛、和田健太、三浦浩美、佐藤正宏、大塚正人
- Organizer
  第62回日本実験動物学会総会
- Place of Presentation
  京都テルサ（京都府京都市）
- Year and Date
  2015-05-28
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] zygote injectionに依らない生殖細胞、胚を標的とした遺伝子導入によるCRISPR/Cas9 genome editingの可能性2015
- Author(s)
  佐藤正宏、大塚正人、中村伸吾、桜井敬之、稲田絵美、齋藤一誠、渡部聡
- Organizer
  第38回日本分子生物学会年会（BMB2015）
- Place of Presentation
  神戸ポートアイランド　（兵庫県神戸市）
- Year and Date
  2015-12-01
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] ex vivo 処置を介さない CRISPR/Cas9 系による遺伝子改変マウス作製法「GONAD」の開発2015
- Author(s)
  高橋剛、Channabasavaiah B Gurumurthy、和田健太、三浦浩美、佐藤正宏、大塚正人
- Organizer
  第 12 回北海道実験動物研究会総会・学術集会
- Place of Presentation
  北海道大学大学院医学研究科臨床講義棟第三講堂　（北海道札幌市）
- Year and Date
  2015-07-11
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] 採卵、顕微注入、胚移植を要しない新規ゲノム編集マウス作製法2015
- Author(s)
  高橋剛、和田健太、三浦浩美、佐藤正宏、大塚正人
- Organizer
  第 29 回モロシヌス研究会
- Place of Presentation
  神戸かんぽの宿有馬　（兵庫県神戸市）
- Year and Date
  2015-07-03
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] GONAD：採卵、顕微注入、移植を要しない新規ゲノム編集マウス作製法2015
- Author(s)
  大塚正人、Channabasavaiah B Gurumurthy、三浦浩美、佐藤正宏、和田健太、高橋剛
- Organizer
  第108回日本繁殖生物学会大会
- Place of Presentation
  宮崎大学木花キャンパス　（宮崎県宮崎市）
- Year and Date
  2015-09-17
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] Development and recent progress in pronuclear injection-based mouse targeted transgenesis method by site-specific recombination2013
- Author(s)
  Masato Ohtsuka
- Organizer
  Genetic Transformation Technologies (plants and animals)
- Place of Presentation
  Vienna, Austria
- Year and Date
  2013-02-14
- Data Source
  KAKENHI-PLANNED-22133002
[Presentation] Neuropathy target esterase（神経障害標的エステラーゼ）遺伝子導入マウスの作成2013
- Author(s)
  加賀谷徹、割田貴之、三浦浩美、大塚正人、大久保朋一、坂部貢、木村穣
- Organizer
  第22回日本臨床環境医学会学術集会
- Place of Presentation
  北里大学白金キャンパス
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] 赤色蛍光強発現tgマウスの唾液腺細胞の蛍光発現第2報2013
- Author(s)
  古川真司、畠山慧、木村仁迪、青松恵美子、石崎明、大塚正人、藤村朗、金野吉晃、清野幸男、三浦廣行
- Organizer
  第72回日本矯正歯科学会大会
- Place of Presentation
  キッセイ文化ホール（長野県松本文化会館）
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] Development and recent progress in pronuclear injection-based mouse targeted transgenesis method by site-specific recombination2013
- Author(s)
  Masato Ohtsuka
- Organizer
  Genetic Transformation Technologies (plants and animals)
- Place of Presentation
  Vienna, Austria
- Invited
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] 受精卵へのiCre mRNA注入によるターゲットトランスジェネシス法（PITT法）の効率改善2013
- Author(s)
  大塚正人、三浦浩美、佐藤正宏、木村穣
- Organizer
  第60回日本実験動物学会総会
- Place of Presentation
  つくば国際会議場
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] Neuropathy target esterase(神経障害標的エステラーゼ)遺伝子導入マウスの作成2013
- Author(s)
  加賀谷徹、割田貴之、三浦浩美、大塚正人、大久保朋一、坂部貢、木村穣
- Organizer
  第22回日本臨床環境医学会学術集会
- Place of Presentation
  東京（日本歯科大学東京短期大学）
- Data Source
  KAKENHI-PROJECT-24651064
[Presentation] トランスポゾンPiggyBacシステムによる複数遺伝子のブタ細胞への同時導入2013
- Author(s)
  郡山美優、稲田絵美、齋藤一誠、三浦浩美、大塚正人、中村伸吾、桜井敬之、渡部聡、三好和睦、佐藤正宏
- Organizer
  第106回日本繁殖生物学会
- Place of Presentation
  東京農工大学農学部府中キャンパス
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] Generation of Knockdown Mice Using Pronuclear Injection-Based Targeted Transgenesis: Advantages and Limitations2013
- Author(s)
  Hiromi Miura, Masato Ohtsuka
- Organizer
  The 27th International Mammalian Genome Conference
- Place of Presentation
  Salamanca, Spain
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] 赤色蛍光強発現 tg マウス唾液腺細胞の蛍光発現に関する研究2013
- Author(s)
  古川真司、畠山慧、堀智樹、石崎明、大塚正人、藤村朗、金野吉晃、清野幸男、三浦廣行
- Organizer
  第55回歯科基礎医学会学術大会・総会
- Place of Presentation
  岡山コンベンションセンター
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] pnpla6遺伝子と有機リン感受性ートランスジェニックマウス作製と遺伝子導入細胞の性質ー2013
- Author(s)
  加賀谷徹,大塚正人,三浦浩美,大久保朋一,田中正史,割田貴之,本杉奈美,坂部貢,梶原景正,加藤明, 吉川枝里,木村穣
- Organizer
  第36 回日本分子生物学会
- Place of Presentation
  神戸
- Data Source
  KAKENHI-PROJECT-24651064
[Presentation] マウス成熟卵胞細胞への生体内遺伝子導入法の開発2012
- Author(s)
  佐藤正宏、赤坂恵理、斉藤一誠、大塚正人、渡部聡
- Organizer
  第35回日本分子生物学会年会
- Place of Presentation
  福岡
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] 腎近位尿細管Kチャネル(TASK2)の酸塩基調節における役割2012
- Author(s)
  安岡有紀子、大塚正人、佐藤雄一、木村穣、河原克雅
- Organizer
  第42回日本腎臓学会東部学術大会
- Place of Presentation
  新潟
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] iCre mRNA注入による次世代型トランスジェニックマウス作製法（PITT）の効率改善2012
- Author(s)
  大塚正人、佐藤正宏、三浦浩美
- Organizer
  第35回日本分子生物学会年会
- Place of Presentation
  福岡国際会議場・マリンメッセ福岡
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] 受精卵への顕微注入法を介したターゲットトランスジェニックマウス作製2012
- Author(s)
  大塚正人
- Organizer
  第14回REG部会
- Place of Presentation
  東京
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] プラスミドDNAの卵管内注入、続く電気穿孔によるマウス着床前胚への遺伝子導入法の開発2012
- Author(s)
  佐藤正宏、赤坂恵理、斉藤一誠、大塚正人、渡部聡
- Organizer
  第59回日本実験動物学会総会
- Place of Presentation
  別府
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] iCre mRNA注入による次世代型トランスジェニックマウス作製法(PITT)の効率改善2012
- Author(s)
  大塚正人、佐藤正宏、三浦浩美
- Organizer
  第35回日本分子生物学会年会
- Place of Presentation
  福岡
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] iCre mRNA注入による次世代型トランスジェニックマウス作製法（PITT）の効率改善2012
- Author(s)
  大塚正人
- Organizer
  第35回日本分子生物学会年会
- Place of Presentation
  福岡国際会議場・マリンメッセ福岡
- Year and Date
  2012-12-11
- Data Source
  KAKENHI-PLANNED-22133002
[Presentation] プラスミドDNAの卵管内注入、続く電気穿孔によるマウス着床前胚への遺伝子導入法の開発2012
- Author(s)
  大塚正人
- Organizer
  第59回日本実験動物学会総会
- Place of Presentation
  B-Con Plaza：ビーコンプラザ
- Year and Date
  2012-05-24
- Data Source
  KAKENHI-PLANNED-22133002
[Presentation] Recombinase及びIntegraseによる部位特異的遺伝子導入効率の比較検討2012
- Author(s)
  大塚正人、三浦浩美、木村穣、猪子英俊
- Organizer
  第59回日本実験動物学会総会
- Place of Presentation
  別府
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] 受精卵への顕微注入法を介したターゲットトランスジェニックマウス作製2012
- Author(s)
  大塚正人
- Organizer
  第14回REG部会
- Place of Presentation
  順天堂大学医学部
- Year and Date
  2012-11-23
- Data Source
  KAKENHI-PLANNED-22133002
[Presentation] 腎近位尿細管Kチャネル（TASK2）の酸塩基調節における役割2012
- Author(s)
  安岡有紀子、大塚正人、佐藤雄一、木村穣、河原克雅
- Organizer
  第４２回日本腎臓学会東部学術大会
- Place of Presentation
  朱鷺メッセ：新潟コンベンションセンター
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] PITT法を利用して作製したノックダウンマウスにおけるノックダウン効果の比較解析2012
- Author(s)
  三浦浩美、佐藤正宏、大塚正人
- Organizer
  第35回日本分子生物学会年会
- Place of Presentation
  福岡
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] プラスミドDNAの卵管内注入、続く電気穿孔によるマウス着床前胚への遺伝子導入法の開発2012
- Author(s)
  佐藤正宏、赤坂恵理、斉藤一誠、大塚正人、渡部聡
- Organizer
  第59回日本実験動物学会総会
- Place of Presentation
  B-Con Plaza：ビーコンプラザ（別府）
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] PITT法を利用して作製したノックダウンマウスにおけるノックダウン効果の比較解析2012
- Author(s)
  三浦浩美、佐藤正宏、大塚正人
- Organizer
  第35回日本分子生物学会年会
- Place of Presentation
  福岡国際会議場・マリンメッセ福岡
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] マウス成熟卵胞細胞への生体内遺伝子導入法の開発2012
- Author(s)
  佐藤正宏、赤坂恵理、斉藤一誠、大塚正人、渡部聡
- Organizer
  第35回日本分子生物学会年会
- Place of Presentation
  福岡国際会議場・マリンメッセ福岡
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] 受精卵への顕微注入法を介したターゲットトランスジェニックマウス作製2012
- Author(s)
  大塚正人
- Organizer
  第14回REG部会
- Place of Presentation
  順天堂大学医学部
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] 間葉系幹細胞の経鼻脳内ホーミングの解析2012
- Author(s)
  城戸直人、高橋沙世、菊池光太、鈴木綾、大久保直登、鄒鶤、田邉千晶、劉俊俊、劉株余、前田智司、大塚正人、藤村朗、佐原資謹、石崎明、駒野宏人
- Organizer
  第78回日本生化学会東北支部例会
- Place of Presentation
  山形
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] Recombinase及びIntegraseによる部位特異的遺伝子導入効率の比較検討2012
- Author(s)
  大塚正人、三浦浩美、木村穣、猪子英俊
- Organizer
  第59回日本実験動物学会総会
- Place of Presentation
  B-Con Plaza：ビーコンプラザ（別府）
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] 再現性良いノックダウン効率を示す、miRNA 発現トランスジェニックマウス作製法の確立2011
- Author(s)
  三浦浩美、猪子英俊、木村穣、大塚正人
- Organizer
  第104回日本繁殖生物学会大会
- Place of Presentation
  いわて県民情報交流センター・アイーナ
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] SOD1H46R遺伝子トランスジェニックALSマウスモデルにおいてグリア線維性酸性蛋白の欠損は疾患をわずかに進行させる2011
- Author(s)
  秦野伸二、吉井康裕、大友麻子、潘雷、大塚正人、岩崎泰雄
- Organizer
  第54回日本神経化学会大会
- Place of Presentation
  石川
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] SOD1H46R遺伝子トランスジェニックALSマウスモデルにおいてグリア線維性酸性蛋白の欠損は疾患をわずかに進行させる2011
- Author(s)
  秦野伸二、吉井康裕、大友麻子、潘雷、大塚正人、岩崎泰雄
- Organizer
  第54回日本神経化学会大会
- Place of Presentation
  石川県山代温泉瑠璃光
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] Vibratory Microinjection System Facilitates Injection of BAC Transgene2011
- Author(s)
  Dilidaer Kudereti, Fujio Miyawaki, Hiroki Haremaki, Tomohiro Yoshizawa, Masato Ohtsuka, Jun Hasegawa
- Organizer
  Mouse Molecular Genetics
- Place of Presentation
  Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] 赤色蛍光強発現Tgマウスの歯科領域研究における有用性2011
- Author(s)
  桑島幸紀、古川真司、安藤禎紀、佐原資謹、石崎明、大塚正人、藤村朗、清野幸男、三浦廣行
- Organizer
  第53回歯科基礎医学会学術大会・総会
- Place of Presentation
  岐阜
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] Pronuclear Injection-Based Mouse Targeted Transgenesis by Site-Specific Recombination2011
- Author(s)
  Masato Ohtsuka
- Organizer
  10th Transgenic Technology Meeting(招待講演)
- Place of Presentation
  TradeWinds Island Grand Resort, USA
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] 受精卵への顕微注入法を介した、部位特異的トランスジェニックマウス作製法2011
- Author(s)
  大塚正人、三浦浩美、木村穣、猪子英俊
- Organizer
  第58回日本実験動物学会総会
- Place of Presentation
  タワーホール船堀
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] 赤色蛍光強発現Tgマウスの歯科領域研究における有用性2011
- Author(s)
  桑島幸紀、古川真司、安藤禎紀、佐原資謹、石崎明、大塚正人、藤村朗、清野幸男、三浦廣行
- Organizer
  第53回歯科基礎医学会学術大会・総会
- Place of Presentation
  長良川国際会議場
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] 遺伝子トランスジェニックALSマウスモデルにおいてグリア線維性酸性蛋白の欠損は疾患進行に若干の影響を与える2011
- Author(s)
  吉井康裕、大友麻子、潘雷、大塚正人、岩崎泰雄、秦野伸二
- Organizer
  第34回日本分子生物学会年会
- Place of Presentation
  横浜
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] GFPuv遺伝子とTypeIIS制限酵素を用いた遺伝子発現ベクターの簡便な構築法とその応用2011
- Author(s)
  三浦浩美、佐藤正宏、猪子英俊、大塚正人
- Organizer
  第34回日本分子生物学会年会
- Place of Presentation
  パシフィコ横浜
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] SOD1H46R遺伝子組み換えALSマウスモデルにおいてグリア線維性産生蛋白の欠損は疾患をわずかに進行させる2011
- Author(s)
  吉井康裕、大友麻子、Pan L.、大塚正人、川邊清一、池田憲、岩崎泰雄、池田穣衛、秦野伸二
- Organizer
  第52回日本神経学会学術大会
- Place of Presentation
  名古屋
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] GFPuv遺伝子とTypeIIS制限酵素を用いた遺伝子発現ベクターの簡便な構築法とその応用2011
- Author(s)
  三浦浩美、佐藤正宏、猪子英俊、大塚正人
- Organizer
  第34回日本分子生物学会年会
- Place of Presentation
  横浜
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] 選択用薬剤を使わない簡便な遺伝子高発現細胞株の取得方法2011
- Author(s)
  佐藤正宏、大塚正人、中村伸吾、櫻井敬之、渡部聡
- Organizer
  第34回日本分子生物学会年会
- Place of Presentation
  パシフィコ横浜
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] 受精卵への顕微注入法を介した、部位特異的トランスジェニックマウス作製法2011
- Author(s)
  大塚正人、三浦浩美、木村穣、猪子英俊
- Organizer
  58回日本実験動物学会総会
- Place of Presentation
  東京
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] GFPuv遺伝子とTypellS制限酵素を用いた遺伝子発現ベクターの簡便な構築法とその応用2011
- Author(s)
  三浦浩美、佐藤正宏、猪子英俊、大塚正人
- Organizer
  第34回日本分子生物学会年会
- Place of Presentation
  パシフィコ横浜
- Data Source
  KAKENHI-PROJECT-19101008
[Presentation] 選択用薬剤を使わない簡便な遺伝子高発現細胞株の取得方法2011
- Author(s)
  佐藤正宏、大塚正人、中村伸吾、櫻井敬之、渡部聡
- Organizer
  第34回日本分子生物学会年会
- Place of Presentation
  横浜
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] 受精卵への顕微注入法を介した、部位特異的トランスジェニックマウス作製法2011
- Author(s)
  大塚正人、三浦浩美、木村穣、猪子英俊
- Organizer
  第104回日本繁殖生物学会大会
- Place of Presentation
  盛岡
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] Vibratory Microinjection System Facilitates Injection of BAC Transgene Mouse Molecular Genetics2011
- Author(s)
  Dilidaer Kudereti, Fujio Miyawaki, Hiroki Haremaki, Tomohiro Yoshizawa, Masato Ohtsuka, Jun Hasegawa
- Organizer
  Wellcome Trust Genome Campus
- Place of Presentation
  Hinxton, Cambridge, UK.
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] 遺伝子トランスジェニックALSマウスモデルにおいてグリア線維性酸性蛋白の欠損は疾患進行に若干の影響を与える2011
- Author(s)
  吉井康裕、大友麻子、潘雷、大塚正人、岩崎泰雄、秦野伸二
- Organizer
  第34回日本分子生物学会年会
- Place of Presentation
  パシフィコ横浜
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] 再現性良いノックダウン効率を示す、miRNA 発現トランスジェニックマウス作製法の確立2011
- Author(s)
  三浦浩美、猪子英俊、木村穣、大塚正人
- Organizer
  第104回日本繁殖生物学会大会
- Place of Presentation
  盛岡
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] SOD1H46R遺伝子組み換えALSマウスモデルにおいてグリア線維性産生蛋白の欠損は疾患をわずかに進行させる2011
- Author(s)
  吉井康裕、大友麻子、Pan L.、大塚正人、川邊清一、池田憲、岩崎泰雄、池田穣衛、秦野伸二
- Organizer
  第52回日本神経学会学術大会
- Place of Presentation
  名古屋国際会議場
- Data Source
  KAKENHI-PROJECT-23700514
[Presentation] 受精卵への顕微注入による、マウス部位特異的遺伝子導入法の開発2010
- Author(s)
  大塚正人、三浦浩美、木村穣、猪子英俊
- Organizer
  第57回日本実験動物学会総会
- Place of Presentation
  京都テルサ(京都)
- Year and Date
  2010-05-12
- Data Source
  KAKENHI-PROJECT-20700368
[Presentation] Pronuclear injection-based targeted transgenesis for in vivo RNAi in the mouse2010
- Author(s)
  三浦浩美、木村穣、猪子英俊、大塚正人
- Organizer
  第33回日本分子生物学会年会第83回日本生化学会大会合同大会
- Place of Presentation
  神戸ポートアイランド(兵庫)
- Year and Date
  2010-12-07
- Data Source
  KAKENHI-PROJECT-20700368
[Presentation] Mouse targeted transgenesis through pronuclear injection2010
- Author(s)
  Masato Ohtsuka
- Organizer
  Mouse Development, Genetics & Genomics
- Place of Presentation
  Cold Spring Harbor, USA
- Year and Date
  2010-10-28
- Data Source
  KAKENHI-PROJECT-20700368
[Presentation] Mouse targeted transgenesis throughpronuclear injection2010
- Author(s)
  Masato Ohtsuka Hiromi Miura, Minoru Kimura, Hidetoshi Inoko
- Organizer
  Mouse Development, Genetics & Genomics2010, CSHL Meetings
- Place of Presentation
  Cold Spring Harbor, USA
- Year and Date
  2010-10-28
- Data Source
  KAKENHI-PROJECT-20700368
[Presentation] マウスMHCクラスIb遺伝子のゲノム構造と発現制御2009
- Author(s)
  大塚正人
- Organizer
  第147回日本獣医学会学術集会
- Place of Presentation
  栃木県総合文化センター(栃木県)
- Year and Date
  2009-04-02
- Data Source
  KAKENHI-PROJECT-20700368
[Presentation] Recombinase-mediated cassette exchange法を用いた、各種蛍光遺伝子発現トランスジェニックマウスの作製2009
- Author(s)
  大塚正人
- Organizer
  第56回日本実験動物学会総会
- Place of Presentation
  大宮ソニックシティ(埼玉県)
- Year and Date
  2009-05-15
- Data Source
  KAKENHI-PROJECT-20700368
[Presentation] マウス受精卵への顕微注入を介した、部位特異的遺伝子導入法の開発2009
- Author(s)
  大塚正人、三浦浩美、荻原早苗、木村穣、猪子英俊
- Organizer
  第32回日本分子生物学会年会
- Place of Presentation
  パシフィコ横浜
- Year and Date
  2009-12-11
- Data Source
  KAKENHI-PROJECT-20700368
[Presentation] マウス受精卵への顕微注入を介した、部位特異的遺伝子導入法の開発2009
- Author(s)
  大塚正人
- Organizer
  第32回日本分子生物学会年会
- Place of Presentation
  パシフィコ横浜(神奈川県)
- Year and Date
  2009-12-11
- Data Source
  KAKENHI-PROJECT-20700368
[Presentation] 人工miRNA発現コンストラクトの作製、検証とノックダウンマウス作製2009
- Author(s)
  三浦浩美、猪子英俊、木村穣、大塚正人
- Organizer
  第32回日本分子生物学会年会
- Place of Presentation
  パシフィコ横浜
- Year and Date
  2009-12-11
- Data Source
  KAKENHI-PROJECT-20700368
[Presentation] Target transgenesis through pronuclear injection.2009
- Author(s)
  大塚正人、三浦浩美、荻原早苗、木村穣、猪子英俊
- Organizer
  第32回日本分子生物学会
- Place of Presentation
  横浜
- Data Source
  KAKENHI-PROJECT-19101008
[Presentation] Recombinase-mediated cassette exchange法を用いた、各種蛍光遺伝子発現トランスジェニックマウスの作製2009
- Author(s)
  大塚正人、荻原早苗、三浦浩美、安藤美貴子、木村穣、猪子英俊
- Organizer
  第56回日本実験動物学会総会
- Place of Presentation
  大宮ソミックシティ
- Year and Date
  2009-05-15
- Data Source
  KAKENHI-PROJECT-20700368
[Presentation] Validation of artificial miRNA expression vectors for generation of knockdown mice.2009
- Author(s)
  三浦浩美、猪子英俊、木村穣、大塚正人
- Organizer
  第32回日本分子生物学会
- Place of Presentation
  横浜
- Data Source
  KAKENHI-PROJECT-19101008
[Presentation] Recombinase-mediated cassette exchange法の確立と、それによるeGFPトランスジェニックマウスの作製2008
- Author(s)
  荻原早苗、木村穣、猪子英俊、大塚正人
- Organizer
  第55回日本実験動物学会総会
- Place of Presentation
  仙台
- Data Source
  KAKENHI-PROJECT-20700368
[Presentation] RMCE法を用いた、赤色蛍光遺伝子tdTomatoトランスジェニックマウスの作製2008
- Author(s)
  三浦浩美、荻原早苗、木村穣、猪子英俊、大塚正人
- Organizer
  第31回日本分子生物学会年会第81回日本生化学会大会合同大会
- Place of Presentation
  神戸
- Data Source
  KAKENHI-PROJECT-20700368
[Presentation] RMCE法を介入して最終的にmarker-freeな遺伝子導入を行う新手法の開発2007
- Author(s)
  大塚正人, 荻原早苗, 今井賢治, 猪子英俊
- Organizer
  第30回日本分子生物学会・第80回日本生化学会合同大会(Biochemistry and Molecular Biology)
- Place of Presentation
  横浜(パシフィコ横浜)
- Year and Date
  2007-12-11
- Data Source
  KAKENHI-PROJECT-19101008
[Presentation] ファイブロサイトに注目した顎関節領域疾患発症機構の解明
- Author(s)
  衣斐美歩、堀江沙和、帖佐直幸、吉田茉莉子、加茂政晴、客本斉子、大塚正人、佐原資謹、藤村朗、石崎明
- Organizer
  第56回歯科基礎医学会学術大会・総会
- Place of Presentation
  福岡国際会議場
- Year and Date
  2014-09-25 – 2014-09-27
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] 赤色蛍光強発現tgマウス唾液腺細胞の蛍光発現に関する研究
- Author(s)
  古川真司　畠山亘　堀智樹　石崎明　大塚正人　藤村朗　金野吉晃　清野幸男　三浦廣行
- Organizer
  第55回歯科基礎医学会学術大会・総会
- Place of Presentation
  岡山コンベンションセンター
- Data Source
  KAKENHI-PROJECT-23592896
[Presentation] Concurrent production of multiple targeted transgenic mouse lines with C57BL/6N genetic background by improved PITT
- Author(s)
  Masato Ohtsuka, Hiromi Miura, Minoru Kimura, Ayako Isotani, Masahito Ikawa, Masahiro Sato, Channabasavaiah Gurumurthy
- Organizer
  12th Transgenic Technology Meeting (TT2014)
- Place of Presentation
  Edinburgh, Scotland, United Kingdom
- Year and Date
  2014-10-06 – 2014-10-08
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] Neuropathy target esterase（神経障害標的エステラーゼ）遺伝子導入マウスの特徴
- Author(s)
  木村穣、加賀谷徹、本杉奈美、割田貴之、三浦浩美、大塚正人、大久保朋一、坂部貢
- Organizer
  第23回日本臨床環境医学会学術集会
- Place of Presentation
  京都大学百周年時計台記念館国際交流ホール
- Year and Date
  2014-06-14 – 2014-06-15
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] NTE(神経障害標的エステラーゼ)遺伝子発現マウスと有機リン代謝
- Author(s)
  山村勇貴、本杉奈美、三浦浩美、大塚正人、大久保朋一、吉野美千代、笹川昇、坂部貢、木村穣
- Organizer
  第37回日本分子生物学会年会
- Place of Presentation
  パシフィコ横浜
- Year and Date
  2014-11-25 – 2014-11-27
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] Efficient generation of conditional alleles in a single-exon gene locus by a double-selection system in mice.
- Author(s)
  Masaru Motojima, Iekuni Ichikawa, Taiji Matsusaka, Masato Ohtsuka.
- Organizer
  第37回日本分子生物学会年会
- Place of Presentation
  横浜
- Year and Date
  2014-11-25 – 2014-11-27
- Data Source
  KAKENHI-PROJECT-25461234
[Presentation] Rosa26遺伝子座位における組織特異的プロモーター活性の検証
- Author(s)
  田村沙亜希、大塚正人、三浦浩美、松阪泰二、安岡有紀子
- Organizer
  第37回日本分子生物学会年会
- Place of Presentation
  パシフィコ横浜
- Year and Date
  2014-11-25 – 2014-11-27
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] Presence/absence of a marker gene in artificial microrna expression constructs affects knockdown efficiencies in a targeted transgenic system
- Author(s)
  Hiromi Miura, Minoru Kimura, Masahiro Sato, Masato Ohtsuka
- Organizer
  12th Transgenic Technology Meeting (TT2014)
- Place of Presentation
  Edinburgh, Scotland, United Kingdom
- Year and Date
  2014-10-06 – 2014-10-08
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] Targeted insertion of short sequences in the mouse ROSA 26 locus using CRISPR/Cas9 system
- Author(s)
  Channabasavaiah Gurumurthy, Rolen Quadros, Donald Harms, Masato Ohtsuka
- Organizer
  12th Transgenic Technology Meeting (TT2014)
- Place of Presentation
  Edinburgh, Scotland, United Kingdom
- Year and Date
  2014-10-06 – 2014-10-08
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] 薬剤副作用の発症機序解明の為の、HLA遺伝子発現細胞とHLAトランスジェニックマウスの作製
- Author(s)
  三浦浩美、大塚正人
- Organizer
  第37回日本分子生物学会年会
- Place of Presentation
  パシフィコ横浜
- Year and Date
  2014-11-25 – 2014-11-27
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] tdTomato遺伝子導入マウス唾液腺由来間葉系幹細胞株の樹立
- Author(s)
  古川真司、桑島幸紀、畠山慧、帖佐直幸、佐藤和朗、大塚正人、石崎明、藤村朗、三浦廣行
- Organizer
  第56回歯科基礎医学会学術大会・総会
- Place of Presentation
  福岡国際会議場
- Year and Date
  2014-09-25 – 2014-09-27
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] マウス骨髄由来異種細胞共培養系の確立
- Author(s)
  滝沢尚希、客本斉子、大久保直登、帖佐直幸、衣斐美歩、加茂政晴、大塚正人、八重柏隆、石崎明
- Organizer
  第56回歯科基礎医学会学術大会・総会
- Place of Presentation
  福岡国際会議場
- Year and Date
  2014-09-25 – 2014-09-27
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] Establishment of a co-culture system for simultaneous proliferation of td-Tomato-expressing mouse bone marrow-derived mesenchymal stem cells and undifferentiated blood cells
- Author(s)
  滝沢尚希、客本斉子、大久保直登、帖佐直幸、衣斐美歩、藤原英明、加茂政晴、大塚正人、八重柏隆、石崎明
- Organizer
  第37回日本分子生物学会年会
- Place of Presentation
  パシフィコ横浜
- Year and Date
  2014-11-25 – 2014-11-27
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] マウス受精卵への顕微注入法を介したRosa26 遺伝子座位へのターゲットトランスジェネシス
- Author(s)
  大塚正人
- Organizer
  第28回モロシヌス研究会
- Place of Presentation
  修善寺総合会館
- Year and Date
  2014-06-27 – 2014-06-28
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] NTE(神経障害標的エステラーゼ)遺伝子発現マウスと有機リン代謝
- Author(s)
  山村勇貴、本杉奈美、三浦浩美、大塚正人、大久保朋一、吉野美千代、笹川昇、坂部貢、木村穰
- Organizer
  第３７回日本分子生物学会年会
- Place of Presentation
  横浜
- Year and Date
  2014-11-25 – 2014-11-27
- Data Source
  KAKENHI-PROJECT-24651064
[Presentation] RepLiCre: Reporter-linked-Cre transgenic mice for efficient use of conditional knockout mouse resources
- Author(s)
  Channabasavaiah Gurumurthy, Masato Ohtsuka, Rolen Quadros, Donald Harms, Andras Nagy, Kent Lloyd
- Organizer
  12th Transgenic Technology Meeting (TT2014)
- Place of Presentation
  Edinburgh, Scotland, United Kingdom
- Year and Date
  2014-10-06 – 2014-10-08
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] オートファジー動態の可視化を目的とした新規トランスジェニックマウスの作出
- Author(s)
  秦野伸二、王、田中政之、林英樹、荻原早苗、岡田千沙、伊藤誠敏、福西菜穂子、飯田裕美、中村彩花、大友麻子、大塚正人
- Organizer
  第37回日本分子生物学会年会
- Place of Presentation
  パシフィコ横浜
- Year and Date
  2014-11-25 – 2014-11-27
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] C57BL/6Nを遺伝的背景に持つターゲットトランスジェネシス用種マウスの作製と評価
- Author(s)
  大塚正人, 三浦浩美, 磯谷綾子, 伊川正人, 佐藤正宏, 木村穣
- Organizer
  第61回日本実験動物学会総会
- Place of Presentation
  札幌コンベンションセンター
- Year and Date
  2014-05-15 – 2014-05-17
- Data Source
  KAKENHI-PROJECT-25290035
[Presentation] マウス骨髄由来異種細胞共培養系の確立.
- Author(s)
  滝沢尚希, 客本斉子, 大久保直登, 帖佐直幸, 衣斐美歩, 加茂政晴, 大塚正人, 八重柏隆, 石崎明
- Organizer
  第５６回歯科基礎医学会学術大会
- Place of Presentation
  福岡国際会議場（福岡市）
- Year and Date
  2014-09-25 – 2014-09-27
- Data Source
  KAKENHI-PROJECT-26462823

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70. 坂牧僚 (40792289)

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71. 仁科博史 (60212122)

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72. 横尾健 (80750629)

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73. ITO Shigeki

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74. MIURA Shingo

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75. MIYATA Kyohei

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76. OZAWA Shogo

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77. KOJIMA Norinaga

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78. HATA Masaki

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79. OMI Maiko

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80. SCHEIFFELE Peter

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81. ULLICH Muller

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82. CHUNHA Shane

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83. TANIMOTO Akihide

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84. KAWAGUCHI Hiroaki

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85. AIDA Tomomi

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86. INADA Emi

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87. 青戸一司

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88. 三好和睦

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89. 平雅之

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90. 齊藤一誠

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91. 齊藤陽子

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92. 客本斉子

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OHTSUKA MASATO 大塚 正人

OHTSUKA Masato 大塚 正人

大塚 正人 オオツカ マサト

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内在性分子で構築されたウイルス様粒子による細胞間mRNA転送の特性の解明と応用Principal Investigator

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Establishment of gene expression control system by modification of introns and its application to analysis of diabetes-related gene functionsPrincipal Investigator

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Development of novel genome editing tool for gene therapy and its application to cancer therapyPrincipal Investigator

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Development of novel gene therapy strategy by insertion of artificial miRNA sequence into intronic region of endogenous gene(Fostering Joint International Research)Principal Investigator

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OHTSUKA MASATO 大塚正人

OHTSUKA Masato 大塚正人

大塚正人オオツカマサト