shirahata akira 白幡晶

… Alternative Names

SHIRAHATA Akira 白幡晶

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Researcher Number	50150107
Other IDs
External Links
Affiliation (Current)	2025: 城西短期大学, ビジネス総合学科, 特任教授
Affiliation (based on the past Project Information) *help	2009 – 2011: Josai University, 薬学部, 教授 2004 – 2006: 城西大学, 薬学部, 教授 2002: Josai University, Department of Biochemistry, Professor, 薬学部, 教授 1996 – 2001: Josai University, Faculty of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 1993 – 1995: Josai Univ., Fac. of Pharm. Sci., Docent, 薬学部, 講師 1988 – 1992: 城西大学, 薬学部, 助手
Review Section/Research Field	Principal Investigator 医薬分子機能学 / Physical pharmacy Except Principal Investigator Physical pharmacy / Biological pharmacy / 医薬分子機能学
Keywords	Principal Investigator ポリアミン / PSD / polyamine oxidase / spermine-N^1-acetyltransferase / spermidine / 細胞容積 / Polyamine / 細胞死 / スペルミジン / スペルミン … More / MALDI-TOFHS / C-terminal labeling / Fluorescent labeling agent for sulfhydryl groups / Conformational change / アミノ酸配列分析 / 蛍光検出HPLC / グアニジン / MALDI-TOF MS / C末端標識法 / 蛍光SH基標識試薬 / コンホメーション変化 / cell proliferation / accumulation of polyamine / Dansylorspermine / inhibitor / fluorescent substrate / assay method / 遷移状態アナログ、 / pyridylamino誘導体、 / 蛍光基質、 / 活性測定法、 / 細胞増殖 / ポリアミン蓄積 / Dansylnorspermine / 阻害剤 / 蛍光基質 / 活性測定法 / NMDA receptor / histon / cell death / arginyltransferase / biotin / photo- affinity labeling / transglutaminase / polyamine / ポリアミントランスポーター / フォトアフィニティーラベル / メランスグルタミナーゼ / アジド / NMDA / アルギニルトランスフェラーゼ / TFO / 蛍光標識 / 遊離型濃度 / NMDA受容体 / ヒストン / アルギニルトランスファーゼ / ビオチン / 光標識 / トランスグルタミナーゼ / Diacetylpolyamine / Polyamine transport / Polyamine oxidase / Cell death / Spermidine / アポトーシス / ジアセチルポリアミン / ポリアミントランスポート / ポリアミン酸化酵素 / ポリアミンアナログ / ポイアミンアナログ / N-acyltransferase(NAT) / アシルアミン / N-acyltransferase (NAT) / アシルスペルミン / アシル基転移反応 / 腸管吸収 / 分子修飾 … More Except Principal Investigator ポリアミン / 活性部位構造 / スペルミジン合成酵素 / アンチザイム / MALDI-TOF-MS / Channel blocker / NMDA receptor / Spermine / Polyamine / NMDA受容体 / スペルミン / Spermidine synthase / スペルミン合成酵素 / 脱炭酸化S-アデノシルメチオニン / ポリアミンアナログ / ポリアミン輸送 / 抗がん効果 / ビスエチルポリアミン誘導体 / Purification of enzyme / Polyamine binding protein / Affinity adsorbent / Substrate specificity / Inhibitor / Active site structure / Spermine synthase / 阻害剤のデザイン / アミノプロピルトランスフェラーゼ / アフィニティ-吸着体 / 拮抗阻害剤 / スペルミジン結合部位 / アミノプロピルトランスフェラ-ゼ / アミノプロピル基転移反応 / ポリアミン結合部位 / 酵素の精製 / ポリアミン親和性蛋白 / アフィニティ吸着体 / 基質特異性 / 阻害剤 / Anthraquinon derivative / Ototoxicity / Aminoglycoside / Regulatory domain / イフェンプロジル / アントラキノン誘導体 / チャネルブロッカー / 難聴 / アミノグリコシド / 調節領域 / decarboxylated S-adenosylmethionine / two functional reagent / SH-reagent / active site structure / amino acid sequence / spermidine synthase / 二機能性試験薬 / 二機能性試薬 / SH試薬 / アミノ酸配列 / Antagonist / Agonist / ビスエチルポリアミン / ポリアンミン / チャンネルブロッカー / アンタゴニスト / アゴニスト / DEcarboxylated S-adnosylmethionine / spermidine / Apoptosis / Hypusine / Polyamine analogues / HTC cells / 5-Carboxyspermine / 標識透導体 / 標識誘導体 / 細胞の増殖 / ジアステレオマーの分離 / スペルミジン / アポトーシス / ハイプシン / HTC細胞 / 5-カルボキシスペルミン / ポリアミン輸送蛋白質 / フレームシフト / デオキシスパーガリン / eIF5A Less

Analysis of Modification with polyamines and investigation of the physiological significancePrincipal Investigator
- Principal Investigator
  
  SHIRAHATA Akira
- Project Period (FY)
  2009 – 2011
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Physical pharmacy
- Research Institution
  Josai University
Proteome analysis as an index of conformational change of proteinPrincipal Investigator
- Principal Investigator
  
  SHIRAHATA Akira
- Project Period (FY)
  2004 – 2006
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Physical pharmacy
- Research Institution
  Josai University
Development of inhibitors for polyamine metabolism including cell deathPrincipal Investigator
- Principal Investigator
  
  SHIRAHATA Akira
- Project Period (FY)
  2000 – 2002
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  医薬分子機能学
- Research Institution
  Josai University
Polyamines as biomodulators - regulation of their contents and their physiological functions
- Principal Investigator
  
  IGARASHI Kazuei
- Project Period (FY)
  1999 – 2000
- Research Category
  
  Grant-in-Aid for Scientific Research (B).
- Research Field
  
  Biological pharmacy
- Research Institution
  Chiba University
ポリアミン代謝拮抗に基づく抗がん剤の分子設計
- Principal Investigator
  
  五十嵐一衛
- Project Period (FY)
  1998
- Research Category
  
  Grant-in-Aid for Scientific Research on Priority Areas (A)
- Research Institution
  Chiba University
Search for proteins as target sof cellular polyaminesPrincipal Investigator
- Principal Investigator
  
  SHIRAHATA Akira
- Project Period (FY)
  1997 – 1999
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  医薬分子機能学
- Research Institution
  Josai University
ビスエチルポリアミン誘導体の抗がん効果
- Principal Investigator
  
  五十嵐一衛
- Project Period (FY)
  1996
- Research Category
  
  Grant-in-Aid for Scientific Research on Priority Areas
- Research Institution
  Chiba University
Search for polyamine agonists and antagonists acting on NMDA receptor.
- Principal Investigator
  
  IGARASHI Kazuei
- Project Period (FY)
  1995 – 1996
- Research Category
  
  Grant-in-Aid for Scientific Research (A)
- Research Field
  
  医薬分子機能学
- Research Institution
  Chiba University
Studies on steric structure of substrate binding site of rat spermidine synthase
- Principal Investigator
  
  SAMEJIMA Keijiro
- Project Period (FY)
  1995 – 1997
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Physical pharmacy
- Research Institution
  Josai University
ビスエチルポリアミン誘導体の抗がん効果
- Principal Investigator
  
  IGARASHI Kazuei
- Project Period (FY)
  1994
- Research Category
  
  Grant-in-Aid for Scientific Research on Priority Areas
- Research Institution
  Chiba University
CELL DEATH AND POLYAMINEPrincipal Investigator
- Principal Investigator
  
  SHIRAHATA Akira
- Project Period (FY)
  1994 – 1995
- Research Category
  
  Grant-in-Aid for General Scientific Research (C)
- Research Field
  
  医薬分子機能学
- Research Institution
  JOSAI UNIVERSITY
Development of methodology for investigation of polyamine-macromolecule interaction in living cells
- Principal Investigator
  
  SAMEJIMA Keijiro
- Project Period (FY)
  1991 – 1993
- Research Category
  
  Grant-in-Aid for General Scientific Research (C)
- Research Field
  
  Physical pharmacy
- Research Institution
  JOSAI UNIVERSITY
Study on Binding Site of Polyamine Binding Protein
- Principal Investigator
  
  SAMEJIMA Keijiro
- Project Period (FY)
  1988 – 1990
- Research Category
  
  Grant-in-Aid for General Scientific Research (C)
- Research Field
  
  Physical pharmacy
- Research Institution
  Josai University

All 2012 2011 2010 2009

All Journal Article Presentation

[Journal Article] A potential estrogen mimetic effect of a bis(ethyl)polyamine analogue on estrogen receptor positive MCF-7 breast cancer cells2012
- Author(s)
  Nayvelt I, John S, Hsu HC, Yang P, Liu W, Das G, Hyvonen MT, Alhonen L, Keinanen TA, Shirahata A, Patel R, Thomas T, Thomas TJ
- Journal Title
  
  Amino Acids
  
  Volume: (in press) Issue: 2-3 Pages: 899-911
- DOI
  10.1007/s00726-011-1005-0
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21590044
[Journal Article] Polyamine metabolism is involved in adipogenesis of 3T3-L1 cells2012
- Author(s)
  Ikumi Ishii, Yoshihiko Ikeguchi, Hiroshi Mano, Masahiro Wada, Anthoby E. Pegg, Akira Shirahata
- Journal Title
  
  Amino Acids
  
  Volume: 42 Issue: 2-3 Pages: 619-626
- DOI
  10.1007/s00726-011-1037-5
- NAID
  120005520696
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21590044
[Journal Article] Enhancement of Gastrointestinal Absorption of Ovalbumin Caused by Spermine Induces an Increase in Plasma Histamine Levels in Mice Sensitized to Ovalbumin2011
- Author(s)
  Yoshiaki Sugita, Koichi Takao, Yoshihiko Sugino, Ryoko Kuwabara, Akira Shirahara
- Journal Title
  
  Biological and Pharmaceutical Bulletin
  
  Volume: 34 Issue: 3 Pages: 415-419
- DOI
  10.1248/bpb.34.415
- NAID
  130000657822
- ISSN
  0918-6158, 1347-5215
- Language
  English
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21590044
[Journal Article] Fluorescent substrates for polyamine catabolic enzymes and transport2011
- Author(s)
  Koichi Takao, Akira Shirahara
- Journal Title
  
  Methods Mol. Biol.
  
  Volume: 720 Pages: 463-474
- DOI
  10.1007/978-1-61779-034-8_30
- ISBN
  9781617790331, 9781617790348
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21590044
[Journal Article] Conformational Stabilization of Rat S-Adenosylmethionine Decarboxylase by Putrescine2010
- Author(s)
  Makiko Wada, Akira Shirahata
- Journal Title
  
  Biological and Pharmaceutical Bulletin
  
  Volume: 33 Issue: 11 Pages: 1800-1805
- DOI
  10.1248/bpb.33.1800
- NAID
  130000402299
- ISSN
  0918-6158, 1347-5215
- Language
  English
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21590044
[Journal Article] Evaluation method for polyamine uptake by N(1)-dansylspermine2010
- Author(s)
  Koichi Takao, Yoshiaki Sugita, Akira Shirahata
- Journal Title
  
  Amino Acid
  
  Volume: 38 Issue: 2 Pages: 533-539
- DOI
  10.1007/s00726-009-0414-9
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21590044
[Journal Article] Identification of the Primary Structure and Post-translational Modification of Rat S-Adenosylmethionine Decarboxylase2010
- Author(s)
  Makiko Wada, Akira Shirahata
- Journal Title
  
  Biological and Pharmaceutical Bulletin
  
  Volume: 33 Issue: 5 Pages: 891-894
- DOI
  10.1248/bpb.33.891
- NAID
  130000248054
- ISSN
  0918-6158, 1347-5215
- Language
  English
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21590044
[Journal Article] Assay of N1-Acetylpolyamine Oxidase Activity with N1,N11-Didansylnorspermine as the Substrate by Ion-Pair Reversed Phase High Performance Liquid Chromatography2010
- Author(s)
  Koichi Takao, Yoshiaki Sugita, Akira Shirahara
- Journal Title
  
  Biological and Pharmaceutical Bulletin
  
  Volume: 33 Issue: 7 Pages: 1089-1094
- DOI
  10.1248/bpb.33.1089
- NAID
  130000300268
- ISSN
  0918-6158, 1347-5215
- Language
  English
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21590044
[Journal Article] A conceptual model of the polyamine binding site of N1-acetylpolyamine oxidase developed from a study of polyamine derivatives2009
- Author(s)
  Koichi Takao, Satoko Shibata, Tomohiro Ozawa, Makiko Wada, Yoshiaki Sugita, Keijiro Samejima, and Akira Shirahata
- Journal Title
  
  Amino Acids
  
  Volume: 37 Issue: 2 Pages: 401-405
- DOI
  10.1007/s00726-008-0168-9
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21590044
[Presentation] スペルミン合成酵素遺伝子改変マウスにおける骨代謝・軟骨代謝異常の解析2011
- Author(s)
  田島彩沙、池口文彦、齋田麻実、中谷祥恵、平澤恵理、石島旨章、和田政裕、真野博、白幡晶
- Organizer
  日本薬学会第131年会
- Place of Presentation
  静岡
- Data Source
  KAKENHI-PROJECT-21590044
[Presentation] エテノ蛍光誘導体化法を用いるS-アデノシルメチオニン脱炭酸化酵素活性測定法の生体試料への応用2011
- Author(s)
  山嵜健一、池口文彦、丹波卓也、白幡晶
- Organizer
  日本薬学会第131年会
- Place of Presentation
  静岡
- Data Source
  KAKENHI-PROJECT-21590044
[Presentation] Electrophoresis analysis for the enhancement in DNA strand-exchange activity of Rad51 by polyamines2011
- Author(s)
  Yoshihiko Ikeguchi, Masaki Sugawara, Motoki Takaku, Kenichi Yamazaki, Anthony E. Pegg, Hitoshi Kurumizaka, Akira Shirahata
- Organizer
  2011 Gordon Research Conference on Polyamines
- Place of Presentation
  米国
- Data Source
  KAKENHI-PROJECT-21590044
[Presentation] 電気泳動によるスペルミンの遺伝子相同組換えに及ぼす影響の解析2011
- Author(s)
  菅原将希、池口文彦、神沼路子、山嵜健一、高久誉大、胡桃坂仁1、白幡晶
- Organizer
  日本薬学会第131年会
- Place of Presentation
  静岡
- Data Source
  KAKENHI-PROJECT-21590044
[Presentation] エテノ蛍光誘導体化法を用いるS-アデノシルメチオニン脱炭酸酵素活性測定法の開発2011
- Author(s)
  山嵜健一、池口文彦、丹波卓也、A. E. Pegg、白幡晶
- Organizer
  日本ポリアミン学会第2回年会
- Place of Presentation
  宇都宮
- Data Source
  KAKENHI-PROJECT-21590044
[Presentation] Histochemistry study on abbrant metabolism in bone and chondrocyte of a spermine-deficient Gy mouse2011
- Author(s)
  Ayasa Tajima, Yoshihiko Ikeguchi, Mami Saita, Sachie Nakatani, Eri Arikawa-Hirasawa, Muneaki Ishijima, Anthony E. Pegg, Masahiro Wada, Hiroshi Mano and Akira Shirahata
- Organizer
  2011 Gordon Research Conference on Polyamines
- Place of Presentation
  米国
- Data Source
  KAKENHI-PROJECT-21590044
[Presentation] マウス及びHepG2細胞を用いる肝臓における脂質蓄積に及ぼすポリアミンの影響2011
- Author(s)
  片倉美佳、下川宗也、岩城孝宏、石井育美、山嵜健一、池口文彦、白幡晶
- Organizer
  日本薬学会第131年会
- Place of Presentation
  静岡
- Data Source
  KAKENHI-PROJECT-21590044
[Presentation] 蛍光基質を用いるHPLC法によるデオキシハイプシン合成酵素活性測定法の開発と応用2010
- Author(s)
  日下部真彦, 池口文彦, 金子宜令, 渡邊亮介, 山嵜健一, 五十嵐一衛, 白幡晶
- Organizer
  日本薬学会第130年会
- Place of Presentation
  岡山
- Data Source
  KAKENHI-PROJECT-21590044
[Presentation] ヒト皮膚線維芽細胞におけるポリアミンの薬物取り込みに及ぼす影響2010
- Author(s)
  橋本崇, 池口文彦, 山嵜健一, 藤堂浩明, 杉林堅次, 白幡晶
- Organizer
  日本薬学会第130年会
- Place of Presentation
  岡山
- Data Source
  KAKENHI-PROJECT-21590044
[Presentation] Spermine SynthaseとGlyceraldehyde-3-phosphate dehydrogenaseとの相互作用解析2010
- Author(s)
  神沼路子, 池口文彦, 高橋幸子, 山嵜健一, 白幡晶
- Organizer
  日本薬学会第130年会
- Place of Presentation
  岡山
- Data Source
  KAKENHI-PROJECT-21590044
[Presentation] Spermine synthase interacts with Glyceraldehyde-3-phosphate dehydrogenase and regulates its activity2010
- Author(s)
  Yoshihiko Ikeguchi, Michiko Kanuma, Ikumi Ishii, Sachiko Takahashi, Kenichi Yamazaki, Anthony E. Pegg and Akira Shirahata
- Organizer
  2010 INTERNATIONAL POLYAMINE CONFERENCE
- Place of Presentation
  御殿
- Data Source
  KAKENHI-PROJECT-21590044
[Presentation] ポリアミンの神経細胞モデルPC12の分化および細胞応答に及ぼす影響2010
- Author(s)
  猿谷幸展, 田代大介, 池口文彦, 田中亨, 山嵜健一, 岡崎真理, 谷覺, 日比野康英, 白幡晶
- Organizer
  日本薬学会第130年会
- Place of Presentation
  岡山
- Data Source
  KAKENHI-PROJECT-21590044
[Presentation] エテノ蛍光誘導体化法を用いるS-アデノシルメチオニン脱炭酸化酵素活性測定法の開発2010
- Author(s)
  山嵜健一, 丹波卓也, 池口文彦, 白幡晶
- Organizer
  日本薬学会第130年会
- Place of Presentation
  岡山
- Data Source
  KAKENHI-PROJECT-21590044
[Presentation] SH標識量に基づくタンパク質のコンホメーション変化のMALDI-TOF MS検出法2010
- Author(s)
  山口直紀, 住吉亜由里, 上田登茂子, 山嵜健一, 池口文彦, 白幡晶
- Organizer
  日本薬学会第130年会
- Place of Presentation
  岡山
- Data Source
  KAKENHI-PROJECT-21590044
[Presentation] HepG2細胞における脂質蓄積に及ぼすポリアミンの影響2010
- Author(s)
  岩城孝宏, 石井育美, 山嵜健一, 池口彦, 白幡晶
- Organizer
  日本薬学会第130年会
- Place of Presentation
  岡山
- Data Source
  KAKENHI-PROJECT-21590044
[Presentation] 疎水性化合物のスペルミン酸化酵素活性に及ぼす影響2010
- Author(s)
  渡邉巧, 池口文彦, 山嵜健一, 五十嵐一衛, 白幡晶
- Organizer
  日本薬学会第130年会
- Place of Presentation
  岡山
- Data Source
  KAKENHI-PROJECT-21590044
[Presentation] 筋肉細胞におけるポリアミンの脂質蓄積に及ぼす影響2010
- Author(s)
  上杉粋世, 石井育美, 坂口花織, 山嵜健一, 池口文彦, 白幡晶
- Organizer
  日本薬学会第130年会
- Place of Presentation
  岡山
- Data Source
  KAKENHI-PROJECT-21590044
[Presentation] HepG2細胞の脂質蓄積におけるポリアミンの役割の解明2009
- Author(s)
  岩城孝宏, 石井育美, 山嵜健一, 池口文彦, 白幡晶
- Organizer
  第53回日本薬学会関東支部会
- Place of Presentation
  埼玉
- Data Source
  KAKENHI-PROJECT-21590044
[Presentation] Spermine Synthaseと相互作用を示すタンパク質の探索2009
- Author(s)
  神沼路子, 池口文彦, 高橋幸子, 山嵜健一, 白幡晶
- Organizer
  第3回トランスグルタミナーゼ研究会 & ポリアミン研究会合同学術集会(同時開催)第82回日本生化学会大会
- Place of Presentation
  神戸
- Data Source
  KAKENHI-PROJECT-21590044
[Presentation] Polyamine analogs affect neurite outgrowth and cellular response of PC12 cells2009
- Author(s)
  Ikeguchi, Y., Tashiro, D., Okazaki, M., Tani, S., Tanaka, T., Hibino, Y. and Shirahata, A.
- Organizer
  Gordon Research Conference on Polyamines 2009
- Place of Presentation
  米国
- Data Source
  KAKENHI-PROJECT-21590044
[Presentation] Effect of Polyamine Synthase Inhibitor on Adipogenesis of 3T3-L1 cells2009
- Author(s)
  Ishii, I., Ikeguchi, Y., Yamazaki, K., Wada, M., Mano, H., Pegg, A. E. and Shirahata, A.
- Organizer
  2009 Gordon Research Seminar on Polyamines及びGordon Research Conference on Polyamines
- Place of Presentation
  米国
- Data Source
  KAKENHI-PROJECT-21590044
[Presentation] ポリアミン合成酵素阻害剤による脂質代謝への影響2009
- Author(s)
  石井育美、池口文彦、山嵜健一、和田政裕、真野博、白幡晶
- Organizer
  第3回トランスグルタミナーゼ研究会 & ポリアミン研究会合同学術集会(同時開催)第82回日本生化学会大会
- Place of Presentation
  神戸
- Data Source
  KAKENHI-PROJECT-21590044

1. IGARASHI Kazuei (60089597)

# of Collaborated Projects: 5 results

# of Collaborated Products: 0 results
2. KASHIWAGI Keiko (80169424)

# of Collaborated Projects: 5 results

# of Collaborated Products: 0 results
3. SAMEJIMA Keijiro (00072413)

# of Collaborated Projects: 3 results

# of Collaborated Products: 0 results
4. NIITSU Masaru (00077976)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
5. KAKINUMA Yoshimi (80134394)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
6. SUGITA Yoshiaki (20255029)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
7. IKEGUCHI Yoshihiko (00364711)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
8. TAKAO Koichi (70337484)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results

shirahata akira 白幡 晶

SHIRAHATA Akira 白幡 晶

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Analysis of Modification with polyamines and investigation of the physiological significancePrincipal Investigator

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Proteome analysis as an index of conformational change of proteinPrincipal Investigator

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Development of inhibitors for polyamine metabolism including cell deathPrincipal Investigator

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Search for proteins as target sof cellular polyaminesPrincipal Investigator

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CELL DEATH AND POLYAMINEPrincipal Investigator

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[Journal Article] A potential estrogen mimetic effect of a bis(ethyl)polyamine analogue on estrogen receptor positive MCF-7 breast cancer cells2012

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[Journal Article] Polyamine metabolism is involved in adipogenesis of 3T3-L1 cells2012

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shirahata akira 白幡晶

SHIRAHATA Akira 白幡晶