Danev Radostin ＤａｎｅｖＲａｄｏｓｔｉｎ

Researcher Number	50415931
Other IDs
Affiliation (Current)	2025: 東京大学, 大学院医学系研究科(医学部), 教授
Affiliation (based on the past Project Information) *help	2021 – 2024: 東京大学, 大学院医学系研究科(医学部), 教授 2018 – 2019: 東京大学, 大学院医学系研究科(医学部), 教授
Review Section/Research Field	Principal Investigator Basic Section 43020:Structural biochemistry-related / Basic Section 43020:Structural biochemistry-related / 0701:Biology at molecular to cellular levels, and related fields Except Principal Investigator Biological Sciences
Keywords	Principal Investigator cryo-EM / data acquisition / single particle analysis / cryo-electron microscopy / deep learning / sample screening / automation / cryo-electron tomography / GPCR / visual proteomics … More / in situ / cryo-FIB / cryo-ET / molecular structure … More Except Principal Investigator TJ-MAPs / TJ-ストランド / アピカル細胞骨格 / TJ-アピカル複合体 / 細胞間バリア / TJストランド / クライオ電顕 / アピカル骨格 / TJMAP / 液-液相分離（LLPS） / アピカル膜 / クライオ電顕トモグラフィー / 細胞骨格 / 上皮細胞 / クローディン / タイトジャンクション / 上皮バリア Less

Intelligent cryo-electron microscopy of G protein-coupled receptorsPrincipal Investigator
- Principal Investigator
  
  Danev Radostin
- Project Period (FY)
  2022 – 2024
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 43020:Structural biochemistry-related
- Research Institution
  The University of Tokyo
New cryo-electron microscopy method for elucidation of G-protein coupled receptor supercomplexesPrincipal Investigator
- Principal Investigator
  
  Danev Radostin
- Project Period (FY)
  2021 – 2022
- Research Category
  
  Grant-in-Aid for JSPS Fellows
- Review Section
  
  Basic Section 43020:Structural biochemistry-related
- Research Institution
  The University of Tokyo
Novel approach focusing on the tight junction-based paracellular barrier combined with the apical barrier toward understanding and manipulating epithelial barriers
- Principal Investigator
  
  月田早智子
- Project Period (FY)
  2019 – 2023
- Research Category
  
  Grant-in-Aid for Specially Promoted Research
- Review Section
  
  Biological Sciences
- Research Institution
  Teikyo University
  Osaka University
Adaptive optics approach for high-throughput, high-resolution cryo-electron microscopy of biological macromolecules and complexesPrincipal Investigator
- Principal Investigator
  
  Danev Radostin
- Project Period (FY)
  2018 – 2019
- Research Category
  
  Grant-in-Aid for Research Activity Start-up
- Review Section
  
  0701:Biology at molecular to cellular levels, and related fields
- Research Institution
  The University of Tokyo

All 2022 2021 2020 2019 2018

All Journal Article Presentation

[Journal Article] Parallel cryo electron tomography on in situ lamellae2022
- Author(s)
  Eisenstein Fabian、Yanagisawa Haruaki、Kashihara Hiroka、Kikkawa Masahide、Tsukita Sachiko、Danev Radostin
- Journal Title
  
  Nature Methods
  
  Volume: 20 Issue: 1 Pages: 131-138
- DOI
  10.1038/s41592-022-01690-1
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-21F20764, KAKENHI-PROJECT-23K23818, KAKENHI-PROJECT-19H05468
[Journal Article] Routine sub-2.5A cryo-EM structure determination of GPCRs2021
- Author(s)
  Danev Radostin、Belousoff Matthew、Liang Yi-Lynn、Zhang Xin、Eisenstein Fabian、Wootten Denise、Sexton Patrick M.
- Journal Title
  
  Nature Communications
  
  Volume: 12 Issue: 1 Pages: 4333-4333
- DOI
  10.1038/s41467-021-24650-3
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-21F20764
[Journal Article] Structure and Dynamics of Adrenomedullin Receptors AM1 and AM2 Reveal Key Mechanisms in the Control of Receptor Phenotype by Receptor Activity-Modifying Proteins2020
- Author(s)
  Liang Yi-Lynn、Belousoff Matthew J.、Fletcher Madeleine M.、Zhang Xin、Khoshouei Maryam、Deganutti Giuseppe、Koole Cassandra、Furness Sebastian G. B.、Miller Laurence J.、Hay Debbie L.、Christopoulos Arthur、Reynolds Christopher A.、Danev Radostin、Wootten Denise、Sexton Patrick M.
- Journal Title
  
  ACS Pharmacology & Translational Science
  
  Volume: 3 Issue: 2 Pages: 263-284
- DOI
  10.1021/acsptsci.9b00080
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K21176
[Journal Article] Activation of the GLP-1 receptor by a non-peptidic agonist2020
- Author(s)
  Zhao Peishen、Liang Yi-Lynn、Belousoff Matthew J.、Deganutti Giuseppe、Fletcher Madeleine M.、Willard Francis S.、Bell Michael G.、Christe Michael E.、Sloop Kyle W.、Inoue Asuka、Truong Tin T.、Clydesdale Lachlan、Furness Sebastian G. B.、Christopoulos Arthur、Wang Ming-Wei、et al
- Journal Title
  
  Nature
  
  Volume: 577 Issue: 7790 Pages: 432-436
- DOI
  10.1038/s41586-019-1902-z
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K08264, KAKENHI-PROJECT-19K21176
[Journal Article] Toward a Structural Understanding of Class B GPCR Peptide Binding and Activation2020
- Author(s)
  Liang Yi-Lynn、Belousoff Matthew J.、Zhao Peishen、Koole Cassandra、Fletcher Madeleine M.、Truong Tin T.、Julita Villy、Christopoulos George、Xu H. Eric、Zhang Yan、Khoshouei Maryam、Christopoulos Arthur、Danev Radostin、Sexton Patrick M.、Wootten Denise
- Journal Title
  
  Molecular Cell
  
  Volume: 77 Issue: 3 Pages: 656-668.e5
- DOI
  10.1016/j.molcel.2020.01.012
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K21176
[Journal Article] Cryo-EM Methodology: Current Aspects and Future Directions2019
- Author(s)
  Radostin Danev, Haruaki Yanagisawa, Masahide Kikkawa
- Journal Title
  
  Trends in Biochemical Sciences
  
  Volume: 印刷中
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K21176
[Journal Article] Cryo-Electron Microscopy Methodology: Current Aspects and Future Directions2019
- Author(s)
  Danev Radostin、Yanagisawa Haruaki、Kikkawa Masahide
- Journal Title
  
  Trends in Biochemical Sciences
  
  Volume: 44 Issue: 10 Pages: 837-848
- DOI
  10.1016/j.tibs.2019.04.008
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K21176
[Journal Article] Improved applicability and robustness of fast cryo-electron tomography data acquisition2019
- Author(s)
  Eisenstein Fabian、Danev Radostin、Pilhofer Martin
- Journal Title
  
  Journal of Structural Biology
  
  Volume: 208 Issue: 2 Pages: 107-114
- DOI
  10.1016/j.jsb.2019.08.006
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K21176
[Presentation] Optimizing cryo-EM for GPCRs and first steps in cryo-tomography2022
- Author(s)
  Radostin Danev
- Organizer
  Frontier of Dynamic Structural Biology, IPR, Osaka
- Invited
- Data Source
  KAKENHI-PROJECT-23K23818
[Presentation] Pushing the performance limits of cryo-EM for membrane proteins2022
- Author(s)
  Radostin Danev
- Organizer
  THE 4TH INTERNATIONAL SYMPOSIUM OF STRUCTURE-BASED DRUG DISCOVERY
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K23818
[Presentation] Optimizing cryo-EM for GPCRs and Parallel cryo electron tomography on in situ lamellae2022
- Author(s)
  Radostin Danev
- Organizer
  ExCells seminar, Okazaki
- Invited
- Data Source
  KAKENHI-PROJECT-23K23818
[Presentation] Optimizing cryo-EM for GPCRs and first steps in cryo-tomography2022
- Author(s)
  Radostin Danev
- Organizer
  The 43rd Annual Meeting of the Japan Society for the Study of Obesity International Satellite Symposium
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K23818
[Presentation] Optimizing cryo-EM for GPCRs and first steps in cryo-tomography2022
- Author(s)
  Radostin Danev
- Organizer
  ARC CCeMMP Symposium, “Cryo-electron microscopy of membrane proteins”
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K23818
[Presentation] What makes a great cryo-EM?2019
- Author(s)
  Radostin Danev
- Organizer
  Future of Cryo-EM Developments Meeting
- Invited
- Data Source
  KAKENHI-PROJECT-19K21176
[Presentation] Phase Plates in Cryo-EM2019
- Author(s)
  Radostin Danev
- Organizer
  EMBL Cryo-EM in Industry and Academia Workshop
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K21176
[Presentation] Cryo-EM of Membrane Receptors2019
- Author(s)
  Radostin Danev
- Organizer
  92nd annual meeting of the Japanese biochemical society
- Invited
- Data Source
  KAKENHI-PROJECT-19K21176
[Presentation] Electron Optics and Image Formation2019
- Author(s)
  Radostin Danev
- Organizer
  Cryo-Electron Microscopy Course at OIST
- Invited
- Data Source
  KAKENHI-PROJECT-19K21176
[Presentation] Phase plates in cryo-EM2019
- Author(s)
  Radostin Danev
- Organizer
  EMBL Cryo-EM in Industry and Academia workshop
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K21176
[Presentation] Recent Cryo-EM Advancements2019
- Author(s)
  Radostin Danev
- Organizer
  1st Cryo-EM Network User Group Meeting
- Invited
- Data Source
  KAKENHI-PROJECT-19K21176
[Presentation] Cryo-EM of Membrane Proteins With and Without Phase Plates2019
- Author(s)
  Radostin Danev
- Organizer
  71st joint JSCB and PSSJ meeting
- Invited
- Data Source
  KAKENHI-PROJECT-19K21176
[Presentation] Practical Performance of the Volta Phase Plate2019
- Author(s)
  Radostin Danev
- Organizer
  Gordon Research Conference on Three Dimensional Electron Microscopy
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K21176
[Presentation] Performance Contributions of Energy Filtering and Phase Plates for Single Particle Analysis of Membrane Proteins2019
- Author(s)
  Radostin Danev
- Organizer
  NIPS EM Workshop 2019
- Invited
- Data Source
  KAKENHI-PROJECT-19K21176
[Presentation] Cryo-EM with and without phase plates2018
- Author(s)
  Radostin Danev
- Organizer
  Japanese Society of Microscopy Soft Materials Branch Meeting
- Invited
- Data Source
  KAKENHI-PROJECT-19K21176

1. 月田早智子 (00188517)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
2. 中村駿 (80882779)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
3. 後藤慎平 (50747219)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
4. 近藤玄 (40243258)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
5. 田村淳 (00362525)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
6. 田中啓雄 (70795905)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
7. 柏原宏香 (20966692)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
8. 足澤悦子 (00446262)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
9. 足立誠 (30335244)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
10. 鈴木貴 (40114516)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
11. 矢野智樹 (20546121)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
12. 入江克雅 (20415087)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
13. EISENSTEIN FABIAN

# of Collaborated Projects: 1 results

# of Collaborated Products: 4 results
14. INOUE Asuka

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results

Danev Radostin Ｄａｎｅｖ Ｒａｄｏｓｔｉｎ

Research Projects

Research Products

Co-Researchers

Intelligent cryo-electron microscopy of G protein-coupled receptorsPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

New cryo-electron microscopy method for elucidation of G-protein coupled receptor supercomplexesPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Novel approach focusing on the tight junction-based paracellular barrier combined with the apical barrier toward understanding and manipulating epithelial barriers

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Adaptive optics approach for high-throughput, high-resolution cryo-electron microscopy of biological macromolecules and complexesPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

[Journal Article] Parallel cryo electron tomography on in situ lamellae2022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Routine sub-2.5A cryo-EM structure determination of GPCRs2021

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Structure and Dynamics of Adrenomedullin Receptors AM1 and AM2 Reveal Key Mechanisms in the Control of Receptor Phenotype by Receptor Activity-Modifying Proteins2020

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Activation of the GLP-1 receptor by a non-peptidic agonist2020

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Toward a Structural Understanding of Class B GPCR Peptide Binding and Activation2020

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Cryo-EM Methodology: Current Aspects and Future Directions2019

Author(s)

Journal Title

Data Source

[Journal Article] Cryo-Electron Microscopy Methodology: Current Aspects and Future Directions2019

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Improved applicability and robustness of fast cryo-electron tomography data acquisition2019

Author(s)

Journal Title

DOI

Data Source

[Presentation] Optimizing cryo-EM for GPCRs and first steps in cryo-tomography2022

Author(s)

Organizer

Data Source

[Presentation] Pushing the performance limits of cryo-EM for membrane proteins2022

Author(s)

Organizer

Data Source

[Presentation] Optimizing cryo-EM for GPCRs and Parallel cryo electron tomography on in situ lamellae2022

Author(s)

Organizer

Data Source

[Presentation] Optimizing cryo-EM for GPCRs and first steps in cryo-tomography2022

Danev Radostin ＤａｎｅｖＲａｄｏｓｔｉｎ